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All Journal Indonesian Journal of Tropical and Infectious Disease Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY Jurnal Kimia Riset Sari Pediatri Paediatrica Indonesiana Folia Medica Indonesiana

Soegeng Soegijanto

Universitas Airlangga

Author-ID : 480895

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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ANTIVIRAL ACTIVITY OF COPPER(II)CHLORIDE DIHYDRATE AGAINST DENGUE VIRUS TYPE-2 IN VERO CELL Sucipto, Teguh Hari; Churrotin, Siti; Setyawati, Harsasi; Kotaki, Tomohiro; Martak, Fahimah; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 4 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Infection of dengue virus (DENV) was number of globally significant emerging pathogen. Antiviral dengue therapies ar importantly needed to control emerging dengue. Dengue virus (DENV) is mosquito-borne arboviruses responsible for causing acute systemic diseases and grievous health conditions in humans. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Copper and copper compounds have more effective in inactivation viruses, likes an influenza virus and human immunodeficiency virus (HIV). Purpose in this project was investigated of Copper(II)chloride Dihydrate antiviral compound were further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measures by Enzyme linked Immunosorbent Assay (ELISA) with selectivity index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for compound. The maximal inhibitory concentration (IC50) of Copper(II)chloride Dihydrate against dengue virus type-2 was 0.13 Î¼g/ml. The cytotoxic concentration (CC50) of compound against Vero cell was 5.03 Î¼g/ml. The SI values for Copper(II)chloride Dihydrate 38.69. Result of this study suggest that Copper(II)chloride Dihydrate demonstated significant anti-DENV-2 inhibitory activities and not toxic in the Vero cells. Copper mechanisms play an important role in the prevention of copper toxicity, exposure to excessive levels of copper can result in a number of adverse health effects, as a result increased reactive oxygen species and oxidative damage to lipid, DNA, and proteins have been observed in human cell culture models or clinical syndromes of severe copper deficiency and inhibition was attributed to released cupric ions which react with cysteine residues on the surface of the protease.

FEVER AS INDICATOR TO SECONDARY INFECTION IN DENGUE VIRAL INFECTION Soegijanto, Soegeng; Nuryandari, Sufiandika; Churrotin, Siti; Sucipto, Teguh Hari
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 1 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue Virus Infections are distributed in tropical and sub-tropical regions and transmitted by the mosquitoes such as Aedes aegypti and Aedes albopictus. Dengue virus can cause dengue fever, dengue hemorrhagic fever and dengue shock syndrome or dengue and severe dengue classified by World Health Organization. Beside it concurrent infection virus salmonella had been found some cases who showed fever more than 7 days. Concurrent infection with two agents can result in an illness having overlapping symptoms creating a diagnostic dilemma for treating physician, such as dengue fever with typhoid fever. The aim of this research is detection of dengue virus and secondary infection with Salmonella typhi in patients suspected dengue virus infection. Detection of dengue virus and Salmonella typhi using immunochromatography test such as NS1, IgG/IgM for dengue virus infection, and IgM/IgG Salmonella and blood culture. The fifty children with dengue virus infection came to Soerya hospital and 17 cases suspected dengue virus infection, five cases showed a positive NS1 on the second day of fever and one case concurrent with clinical manifestation of convulsi on the third days of fever there were five cases only showed positive. It was showed in this study that on the fourth to six day of fever in dengue virus infection accompanied by antibody IgM & IgG dengue. There were 12 cases showed the clinical manifestation of concurrent dengue viral infection and Salmonella, all of them showed a mild clinical manifestation and did not show plasma leakage and shock. In this study we found the length of stay of concurrent Dengue Virus Infection and Salmonella infection is more than 10 days. These patients were also more likely to have co-existing haemodynamic disturbances and bacterial septicaemia which would have required treatment with inotropes and antibiotics. This idea is very important to make update dengue viral management to decrease mortality in outbreak try to gain new prevention method before the occurrence of outbreak.

INHIBITORY ACTIVITY OF COBALT(II)–MORIN COMPLEX AGAINST THE REPLICATION OF DENGUE VIRUS TYPE 2 Sucipto, Teguh Hari; Churrotin, Siti; Setyawati, Harsasi Setyawati; Mulyatno, Kris Cahyo; Amarullah, Ilham Harlan; Ueda, Shuhai; Kotaki, Tomohiro; Sumarsih, Sri; Wardhani, Puspa; Bendryman, Sri Subekti; Aryati, Aryati; Soegijanto, Soegeng; Kameoka, Masanori
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 6 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. Antidengue treatments are urgently required to control the emergence of dengue. DENV is a mosquito-borne disease responsible for acute systemic diseases and serious health conditions. DENVs were distributed in the tropical and sub-tropical areas and transmitted to humans by Aedes agypty and Aedes albopictus. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is an important alternative for the characterization and development of drugs. Metal–organic compounds were reported to exhibit fungicidal, bactericidal, and antiviral activities its inhibitory activity was not significant, at high concentration it was more toxic to replicating cells than to stationary cell monolayers of Vero cells. The aim of this study is to investigate the antiviral effects of Cobalt(II)–Morin complex. This compound was further investigated for its inhibitory effect on the replication of DENV-2 in Vero cells. The replication of DENV was measured by enzyme-linked immunosorbent assay and the value of selectivity index (SI). SI was determined as the ratio of the 50% cytotoxic concentration (CC50) to the 50% inhibitory concentration (IC50). The IC50 value of the Cobalt(II)–Morin complex for DENV-2 was 3.08 µg/ml, and the CC50 value of the complex for Vero cells was 3.36 µg/ml; thus, the SI value was 1.09. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of Cobalt(II)–Morin complex and its high toxicity in Vero cells. Further studies are not required before Co(II)–Morin can be applied in the treatment of DENV-2 infections.

RNA ISOLATION OF DENGUE VIRUS TYPE 1 WITH DIFFERENT PRECIPITATION SOLVENTS: DIMETHYL SULFOXIDE, ACETONE, AND ETHANOL 70% Maharani, Anisa; Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Wardhani, Puspa; Aryati, Aryati; Ueda, Shuhai; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 3 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue Hemorrhagic Fever (DHF) is caused by dengue viruses that belong to Flaviviridae. The disease is known to be caused by 4 types of dengue viruses, namely DENV-1, DENV-2, DENV-3, and DENV-4 associated with antigenic. Dengue virus is a virus RNA that causes illness with clinical manifestations of Dengue Fever, Dengue Hemorrhagic Fever and Dengue Shock Syndrome. The aim of research was to determine the effectiveness of dimethyl sulfoxide, acetone, and ethanol 70% as precipitation solvent in the process of RNA isolation. The method used was Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) with specific primers for dengue virus type 1 (DENV-1). RNA isolation can be done easily using an RNA Isolation Kit. Use of RNA Isolation Kit results in a purer RNA isolate from contaminants and from RNA degradation. In generally the isolation is using cold ethanol / alcohol with concentration 90-95%. Ethanol / Alcohol does not dissolve RNA and light density of alcohol lighter than water makes RNA rise and hover on the surface. In RNA isolation solvent precipitation that used are acetone, ethanol 70%, and DMSO. In qualitative RNA measurements using agarose gel electrophoresis and was examined under the UV light-illuminator and quantitative RNA measurements using Nanodrop spectrophotometry with absorbance ratio at 260/280 and 260/230 showed a good result indicated by the appearance of the band on electrophoresis results in PCR. While the measurement quantitatively is showed that there was still protein contamination but the results are quite good because it does not much different from the ratio set in the reference. Acetone, ethanol 70%, and DMSO can be used as a substitute of 96% ethanol in the process of RNA isolation in DENV-1 virus and can also be applied to other dengue virus because the structure of the 4th antigen serotype is very similar one with the other and no effect.

ANTI-DENGUE TYPE 2 VIRUS ACTIVITIES OF ZINC (II) COMPLEX COMPOUNDS WITH 2-(2,4 -DIHYDROXYPHENYL)-3,5,7-TRIHYDROXYCROMEN-4-ONE LIGANDS IN VERO CELLS Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Sumarsih, Sri; Wardhani, Puspa; Aryati, Aryati; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue virus (DENV) is a disease that is transmitted through Aedes aegypti and Aedes albopictus mosquitoes, and is spread in tropical and sub-tropical regions. Now, dengue or antiviral vaccines for humans do not yet exist, but there are great efforts to achieve this goal. Complex compounds are reported to fungicidal, bactericidal and antiviral activity. Antiviral activity against DENV is an important alternative to the characterization and development of drugs candidate. The purpose of this study was to study zinc(II) compounds with 2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxycromen-4-one ligand on DENV-2 replication in Vero cells. Vero cell lines (African green monkey kidney) was used in this study, maintained and propagated in Minimum Essential Eagle Medium containing 10% fetal bovine serum at 37°C in 5% CO2. The activity of dengue virus was carried out by enzyme-immunosorbent assay (ELISA) method and CellTiter96® Non-Radioactive Proliferation. The value of activity inhibition (IC50) of complex compounds with variations of mol metal: ligand 1:2, 1:3, and 1:4 against dengue virus type 2 (DENV2) was 2.44 Î¼g/ml, 2.75 Î¼g/ml, respectively and 2.00 Î¼g/ml, also the toxicity value (CC50) of complex compounds with variation mol metal: ligand 1:4 for Vero cells is 3.59 Î¼g/ml. The results of this study were indicate that these properties have been shown to inhibit anti-dengue type 2 virus (DENV-2), but are also toxic in Vero cells. Including previous study about complex compound interaction with dengue virus type 2 activity, Zn(II) more reactive compound then Cu(II), and Co(II). The comparison with Cu(II) complex compound, it has been revealed that Co(II) and Zn(II) is more toxic, was found to be nontoxic to human erythrocyte cells even at a concentration of 500 Î¼g/ml.

Eï¬€ect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell Sucipto, Teguh Hari; Wibrianto, Aswandi; Martak, Fahimah; Churrotin, Siti; Amarullah, Ilham Harlan; Setyawati, Harsasi; Wardhani, Puspa; Aryati, Aryati; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 8 No. 3 (2020)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v8i3.11776

Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many eï¬€orts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitory eï¬€ect on the replication of DENV-2 in cell culture. DENV replication was measured by antiviral activity assay and cytotoxicity assay. The inhibitory activity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by Viral ToxGloTM Assay. The cytotoxicity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by CellTiter96® AQuoeus assay. The inhibitory concentration (IC50) of Zinc(II)-2,4,5-triphenyl- 1H-imidazole against dengue virus type-2 was 34.42 Î¼g/ml. The cytotoxic concentration (CC50) of compound against Vero cell was <100 Î¼g/ml. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole complex and its high toxicity in Vero cells. Further studies are not required before investigated Zinc(II)-2,4,5-triphenylimidazole can be applied in the treatment of DENV-2 infections

Knowledge and Attitudes of Dengue Virus Infection Transmission and Its Relationship with Eradication Action Program in Surabaya, Indonesia Juliasih, Ni Njoman; Sucipto, Teguh Hari; Sari, Reny Mareta; Nuha, Zakiyathun; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 10 No. 2 (2022)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v10i2.36477

Dengue virus infection is caused by a dengue virus transmitted through mosquito bites from species Aedes albopictus and Aedes aegypti. The Ministry of Health takes action to reduce the prevalence of DHF by regulating the management of PSN 3M Plus. This study aimed to determine the knowledge, attitude, and compliance with the management of PSN 3M Plus strategies of those living in Surabaya. A cross-sectional population-based google form questionnaire was conducted in January 2022 for four weeks (January 3, 2022, to January 29, 2022). Based on the bivariate analysis, gender and age of respondents were no relationship between compliance with the PSN 3M Plus (p-value >0.05). The results also showed no relationship between education and adherence to PSN 3M Plus (p-value > 0.05). However, based on previous studies, people with higher education showed better compliance. Public knowledge and attitude about the dengue virus and its transmission process can be increased by developing, modifying, and intervening in the people controlling dengue virus infection. Most people of Surabaya believe that dengue prevention is the complete responsibility of every people. Based on the bivariate analysis, the characteristics of respondents had no relationship with the PSN 3M Plus compliance (p-value > 0.05). Knowledge and attitudes of the Surabaya people toward PSN 3M Plus are still good. However, the characteristics of the respondents did not significantly affect their knowledge and attitudes

Epidemiology Study of Dengue Virus In Surabaya, Bogor, and Bangkalan, Indonesia 2008-2018 Soegijanto, Soegeng; Sucipto, Teguh Hari; Mulyatno, Kris Cahyo; Churrotin, Siti
Folia Medica Indonesiana Vol. 56, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Dengue virus (DENV) is mosquito-borne viral diseases, transmitted by the vector mosquitoes such as Aedes sp. Infection with four serotypes of DENV-1 to 4. Indonesia, dengue haemorrhagic fever (DHF) was first recognized in 1968 in the cities of Jakarta and Surabaya. In 2007, we started DENV surveillance in Surabaya supported by the joined program of the Japan Initiative for Global Research Network on Infectious Disease (J-GRID) established the Indonesia-Kobe University Collaborative Research Center for Emerging and Reemerging Infectious Diseases (CRC-ERID). The results of serotype and genotype, in Surabaya and Bangkalan are similar with previous result in Indonesia, but especially in Bogor similar with Japan 2014. This study showed the importance of continuous virus surveillance in dengue endemic areas, in order to understand the dynamic of dengue infection disease in Indonesia.

Correlation Between Platelet and Hematocrite Values with Duration Fever in Dengue Hemorrhagic Fever (DHF) Patients at Dr. Soedomo General Hospital, Trenggalek Alelo, Richardo Reynaldi Sakka; Sucipto, Teguh Hari; Soegijanto, Soegeng; Wardhani, Puspa
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.73388

Dengue Hemorrhagic Fever (DHF) is an endemic disease in Indonesia with laboratory manifestations such as thrombocytopenia and hemoconcentration. The duration of fever in DHF can be an indicator of disease severity, but its relationship to laboratory parameters such as platelets and hematocrit requires further study. The purpose of this research is to determine the relationship between platelet and hematocrit values and the duration of fever in DHF patients at Dr. Soedomo Trenggalek Regional General Hospital. This study used a cross-sectional design with secondary data from the medical records of 71 dengue fever patients. Statistical analysis used the Spearman correlation test to assess the relationship between platelets and hematocrit with fever duration. 54.93 % of patients were male, with the largest age group being young adults (26–45 years, 39.44%). The maximum duration of fever was 5 days (54.93 %). There was a weakly significant relationship between decreased platelets and increased duration of fever (p = 0.025; r = 0.266). However, there was no significant relationship between hematocrit and duration of fever (p = 0.359; r = -0.111). Platelets can be used as an indicator to estimate the duration of fever in dengue fever patients, while hematocrit did not show a significant relationship. These findings support the importance of platelet monitoring for the clinical management of dengue fever, although factors such as fluid therapy and disease phase need to be considered in hematocrit interpretation.

Co-Authors Abdul Latief Azis Alelo, Richardo Reynaldi Sakka Amaliah Labiqah, Amaliah Amor Peraten Ginting, Amor Peraten Anisa Maharani Arif Ansori, Arif Arif Nur Muhammad Ansori Aryati Aryati Atsushi Yamanaka, Atsushi Atsusi Yamanaka, Atsusi Atushi Yamanaka, Atushi Budi Setiawan Budi Utomo Desiana W Sari, Desiana W Desiana W. Sari, Desiana W. Desiana Wikanesthi, Desiana Dwi Atmaji Soejono Dyah Wikanesthi, Dyah Eiji Konichi, Eiji Eiji Konishi, Eiji Eryk Hendrianto Eva Chilvia, Eva Fahimah Martak Fedik A Rantam Harsasi Setyawati Hartati, Edim Hebert Adrianto Helen Susilowati, Helen Ilham Harlan Amarullah Ismoedijanto Kris Cahyo Mulyanto, Kris Cahyo Kris Cahyo Mulyatno, Kris Cahyo Lely Kurniasari, Lely Maharani, Anisa Masa Nori Kamioka, Masa Nori Masanori Kameoka, Masanori Ni Njoman Juliasih Novia Faridatus Sholihah Nuha, Zakiyathun Nur Ahwanah, Nur Nuryandari, Sufiandika Oedojo Soedirham Parwati Setiono Basuki Pemta Deka, Pemta Puspa Wardhani Reny Mareta Sari, Reny Mareta Risky Vitria Prasetyo Rizqidhana Juliana Putri S.Pd. M Kes I Ketut Sudiana . Sary, Dian Dwi Shifa Fauziyah Shuhai Ueda Siti Churotin, Siti Siti Churrotin, Siti Soetjipto Soetjipto Sri Subekti Sri Sumarsih Teguh Hari Sucipto, Teguh Hari Teguh Sucipto, Teguh Tomohiro Kotaki, Tomohiro Ueda, Shuhai Wardhani, Puspa Wibrianto, Aswandi Widodo Darmowandowo, Widodo Yoes Priyatna Yovilianda Maulitiva Untoro

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