Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : Bioma : Jurnal Biologi Indonesia

INDUKSI MUTASI PADA PISANG (Musa sp. - ABB) cv. KEPOK DENGAN IRADIASI GAMMA SECARA IN VITRO Masykuroh, Luthfia; Adisyahputra, Adisyahputra; Indrayanti, Reni
Bioma Vol. 12 No. 1 (2016): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (534.258 KB) | DOI: 10.21009/Bioma12(1).3

Abstract

Banana (Musa sp. - ABB) cv. Kepok is one of type banana processed that have a very potential commodities fruit developed to support food survival. The purpose of this study was to knowing the effect of gamma irradiation on the growth of banana plants cv. Kepok in vitro. This study was conducted in October 2014 – October 2015 in Plant Tissue Culture Laboratory, Biological – Science UNJ. The methods used was experiment with fully randomized design. Factors that tested was 6 gamma irradiation doses (0, 20, 30, 40, 50, 60 Gy) with 10 repetition. Observation of phenotypic generate diverse characters on the growth of the number of shoots and leaves. Gamma irradiation dose of 50 Gy is doses most inhibits the growth of character. Mutations that occur in banana plantlets cv. Kepok generated by the treatment doses gamma irradiation induced mutation is random.
PERKECAMBAHAN 4 AKSESI JEWAWUT (Setaria italica (L.) P. Beauv) PADA KONDISI CEKAMAN KEKERINGAN ARTIFISIAL Mapikasari, Septiani; Adisyahputra, Adisyahputra; Indrayanti, Reni
Bioma Vol. 13 No. 1 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.192 KB) | DOI: 10.21009/Bioma13(1).6

Abstract

Developing of jewawut cultivation as an alternative source of carbohydrates is one of the efforts to prevent food insecurity. Drought conditions and the availability of drought-tolerant seeds became one of the problems in the development of jewawut cultivation. The purpose of these experiments were to evaluate jewawut response to drought stress simulations at germination phases and to obtain accessions tolerant to drought stress. Drought stress is performed indirectly (PEG 6000 selective media). The research was done in Laboratory of Physiology of Faculty of Mathematic and Science, UNJ from February until July 2017. The experiments were done with a completely randomized design. Parameters of germination were analyzed with Anova test and continued by Duncan Multiple Range Test (DMRT). Lethal doses of PEG reducing 50% of germination (LD50) were 23,25%, with the quadratic equation y = 1,12-2,72x. The results base on germination phase, Buru merah as drought tolerance accessions, Polman merah and Polman kuning as medium tolerance accession, and Buru kuning as susceptible accessions.
UJI KETAHANAN NILAM TERHADAP Synchytrium pogostemonis PENYEBAB PENYAKIT BUDOK DAN POTENSI PENGENDALIANNYA DENGAN PESTISIDA NABATI Yuliyanti, Tri; Hartati, Sri Yuni; Indrayanti, Reni
Bioma Vol. 13 No. 2 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.166 KB) | DOI: 10.21009/Bioma13(2).5

Abstract

Nilam (Pogostemon cablin Benth.) merupakan tanaman semak penghasil minyak atsiri yang memiliki nilai ekonomi yang tinggi. Budidaya tanaman nilam memiliki kendala yaitu adanya serangan kapang Synchytrium pogostemonis penyebab penyakit budok. Tujuan dari penelitian ini adalah untuk mengetahui ketahanan 3 varietas nilam terhadap penyakit budok, dan mengetahui efektivitas dari formula tunggal dan campuran minyak mimba dan seraiwangi terhadap kejadian penyakit budok dan pengaruhnya pada pertumbuhan tanaman. Penelitian ini terdiri dari dua percobaan: 1) Efektivitas metode infeksi dan uji ketahanan tiga varietas nilam terhadap penyakit budok di rumah kaca, dan 2) Uji efektivitas minyak atsiri formula tunggal maupun campuran mimba dan seraiwangi sebagai pestisida nabati terhadap penyakit budok. Hasil percobaan efektivitas metode infeksi menujukkan bahwa metode perendaman akar tanaman dalam inokulum budok selama satu jam lebih efektif menginfeksi penyakit budok dibandingkan dengan metode penyiraman ke media tanam. Hasil uji ketahanan nilam varietas Sidikalang, Patchoulina 1 dan Patchoulina 2 menunjukkan bahwa varietas Patchoulina 1 lebih rentan terhadap penyakit budok karena menimbulkan keterjadian penyakit (KeP) dan keparahan penyakit (KP) hingga 20%, dengan karekter pertumbuhan tinggi tanaman yang lebih rendah dibandingkan varietas lain yang diuji. Sedangkan diantara minyak atsiri yang diujikan, formula minyak atsiri campuran (mimba dan seraiwangi) konsentrasi 0.5% lebih efektif dalam mengurangi penyakit budok, namun efektivitasnya masih lebih rendah dibandingkan dengan perlakuan fungisida benomil 0,3% dan bubur bordo 0,1%. Aplikasi formula minyak atsiri secara signifikan tidak berpengaruh negatif terhadap pertumbuhan nilam, sehingga formula campuran (mimba dan seraiwangi) 0,5% dapat digunakan untuk mengontrol penyakit budok pada tanaman nilam.
MULTIPLICATION AND ACCLIMATIZATION OF BANANA VARIANT CV. AMPYANG (Musa acuminata, AAA) PUTATIVE RESISTANCE TO FUSARIUM WILT Indrayanti, Reni; Yanti, Fitri; Adisyahputra, Adisyahputra; Dinarti, D; Sudarsono, Sudarsono
Bioma Vol. 14 No. 1 (2018): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.564 KB) | DOI: 10.21009/Bioma14(1).3

Abstract

Abstract Plants resistance to Fusarium wilt can be developed by mutation breeding and in vitro selection techniques. The objectives of this research were to evaluated growth and development of banana plantlets cv. Ampyang (Musa acuminata, AAA) resistance to Fusarium wilt. The plant material used in this study was 9 (nine) code clones banana cv. Ampyang result from mutations induced by gamma irradiation (30, 45, and 50 Gy) and in vitro - in vivo selection, a collection of Plant Tissue Culture Laboratory Department of Biology UNJ. Plantlets multiplied for four months on Murashige and Skoog supplemented with2.25 mgL-1 Benzyl amino purine and 0.22 mgL-1 Thydiazuron and 0.175 mgL-1 Indole 3 acetyc-acid . Acclimatization and evaluation of plantlet growth and development in a greenhouse showed that percentage of survival rate of plantlet were ranging from 42.9 – 100%. There was phenotypic variation among those plantlet investigated in quantitative and qualitative character. Plantlet regenerated from clone A regenerated from gamma irradiation 30 Gy and after in vitro selection of Foc from isolate Medan showed significantly the highest number of leave and fresh weight. The less number of roots length and other phenotypic character were produced from clone B, D and F. All of the regenerated plantlets were successfully transferred into soil and they would be used to evaluate the existence of variants in a greenhouse. Keywords: phenotypic variation, induce mutation and in vitro selection, shoot multiplication, Foc isolates from Medan
Co-Authors A.A. Ketut Agung Cahyawan W Abd. Rasyid Syamsuri Abdul Wahid Abdul Wahid Achmad, Farhana Faridah Adisayhputra, Adisayhputra Adisyahputra Adisyahputra Adisyahputra Adisyahputra Adisyahputra Adisyahputra, Adisyahputra Adisyahputra, A AGUNG SEDAYU Amala, Azizatul Amanda, Debby Viola Amanda, Debby Viola Asep Ali Asep Ali Asep Ali, Asep Asharo, Rizak Koen Asiani, Nia Asiani, Nia Bambang Sukmadi Bambang Sukmadi Bedah Rupaedah Bedah Rupaedah Bedah Rupaedah, Bedah Damayanti, Aldira Putri Debby Viola Amanda Debby Viola Amanda Desnurvia, Riza Dinarti, D Dono Wahyuno Dwi Eldina Dwi Ningsih Susilowati Elsa Lisanti Ervan Nurkholis Febrian, Reyno Ahmad Firmansyah, Taufik Firmansyah, Taufik Fitri Yanti Handayani, Gina Ifa Manzila Khairatunnisa, Fauziah Mahmud Sugianto Mahmud Sugianto Mahmud Sugianto, Mahmud Mapikasari, Septiani Masykuroh, Luthfia Mieke Miarsyah Mieke Miarsyah Nabilah, Saskia NFN Sukamto Nia Asiani Nia Asiani Novi Listiana NOVITASARI, Ayu Nurhajati A. Mattjik Nurhajati A. Mattjik Pinta Omas Pasaribu Putri, Hilda Arsyah Eka Rafika Yuniawati Rahayu Rahayu Raihan, Eldrian Daffa Ridho Kurniati Rizal Koen Asharo Rizal Koen Asharo Rizkawati, Vina Rizky Priambodo Saputro, Sigit Setiawan, Asep S Siti Fatimah Sri Yuni Hartati Sudarsono Sudarsono Sukmadi, Bambang Sukmadi, Bambang Sutanto, Agus Tampanguma, Raymond Rayhand Taufik Firmansyah Taufiq Firmansyah Tri Puji Priyatno Tri Yuliyanti Utari, Raysita Vina Rizkawati Wardana, Nurul Assyifa Wulandari, Nadia Fitria YULIA IRNIDAYANTI