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Pelatihan Budidaya Pakcoy Dengan Sistem Hidroponik Rakit Apung Sebagai Upaya Memanfaatkan Pekarangan Sempit Di Rawamangun, Jakarta Timur Pinta Omas Pasaribu; Reni Indrayanti; Adisyahputra; Rizal Koen Asharo; Rizky Priambodo; Vina Rizkawati; Yulia Irnidayanti
Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Vol 1 (2020): PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT - SNPPM2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.456 KB)

Abstract

Abstract Until this day, the use of yard land is still not optimal, thus the development of various innovations related to home yards is also limited. The houses in the area of neighborhood (RT) 11 Hamlet (RW) 03 Rawamangun Urban Village East Jakarta have narrow yards. One alternative to increase limited space productivity is through the use of simple hydroponic techniques. The principle of floating raft hydroponics is growing plants with water as a medium while floating on a nutrient solution. Its simple implementation allows it to be applied to limited land space. The purpose of this community service is to convey information and training on the use of the floating raft hydroponic system which can be used as an alternative to increase the productivity of the pakcoy vegetable plant. Community service activities were carried out through lectures, discussions and direct demonstrations of cultivating pakcoy with a floating raft hydroponic system in the land of the residents of RT 11 RW 03. Presentations were carried out by explaining an easy and practical method to cultivate hydroponic pakcoy plants and provide an explanation of the benefits of hydroponic plants. The pakcoy cultivation consists of 4 stages, namely the stage of seeding, transfer to floating rafts, maintenance and harvesting. The implementation of the activity went smoothly and received very positive responses from the participants, seen from the number of questions received and the enthusiasm of the participants during the activity. The participants gave very positive response to the information provided. The activity ended with the provision of hydroponic pakcoy vegetables, floating raft kits, and transplanted seeds into floating rafts. Abstrak Pemanfaatan lahan pekarangan pada saat ini masih belum optimal, sehingga pengembangan berbagai inovasi yang terkait dengan lahan pekarangan juga terbatas. Wilayah Rt 11 Rw 03 Kelurahan Rawamangun, merupakan salah satu wilayah di Jakarta Timur yang memiliki lahan pekarangan yang sangat sempit. Salah satu alternatif yang dapat dilakukan dalam meningkatkan produktivitas lahan yang terbatas yaitu melalui pemanfaatan teknik hidroponik yang sederhana. Hidroponik rakit apung memiliki prinsip menanam tanaman dengan media air dalam keadaan diapungkan di atas larutan nutrisi. Implementasinya yang sederhana sangat memungkinkan untuk diterapkan pada lahan yang terbatas. Tujuan dari pengabdian masyarakat ini adalah menyampaikan informasi dan pelatihan penggunaan sistem hidroponik rakit apung yang dapat dijadikan sebagai alternative untuk meningkatkan produktivitas tanaman sayuran pakcoy. Kegiatan pengabdian dilakukan melalui ceramah, diskusi dan demonstrasi langsung budidaya tanaman pakcoy dengan sistem hidroponik rakit apung di lahan pekarangan warga Rt 11 Rw 03. Kegiatan presentasi dilakukan dengan penjelasan cara budidaya tanaman pakcoy hidroponik yang mudah dan praktis serta penjelasan akan manfaat dari tanaman hidroponik. Pembuatan budidaya tanaman pakcoy terdiri dari 4 tahapan yaitu tahap pembibitan, pemindahan ke bak rakit apung, perawatan dan panen. Pelaksanaan kegiatan berjalan lancar dan mendapat tanggapan yang sangat positif dari para peserta, dilihat dari banyaknya pertanyaan yang muncul serta antusiasme peserta selama kegiatan. Respon peserta terhadap informasi yang diberikan sangat baik. Kegiatan diakhiri dengan pemberian tanaman sayuran pakcoy hasil hidroponik, kit rakit apung, dan bibit yang sudah dipindah tanam kedalam bak rakit apung.
Induksi Mutasi Pisang Cv. Tanduk secara In Vitro dan Deteksi Awal Ketahanan Tunas Varian terhadap Fusarium oxysporum f.sp cubense Reni Indrayanti; Khairatunnisa, Fauziah; Adisyahputra; Sedayu, Agung; Asharo, Rizak Koen
Jurnal Hortikultura Indonesia (JHI) Vol. 12 No. 2 (2021): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.12.2.147-156

Abstract

Pisang Tanduk (AAB) merupakan jenis pisang olahan dengan ciri-ciri buah yang tidak cepat busuk, namun menghasilkan jumlah sisir relatif sedikit. Tujuan penelitian untuk menginduksi mutasi pisang Tanduk dan mengidentifikasi klon pisang yang resisten terhadap infeksi Fusarium oxysporum f.sp cubense (Foc). Pisang Tanduk ditanam pada media MS dengan penambahan 6.5 mg L-1 BAP, 1.175 mg L-1 IAA, dan 0.22 mg L-1 TDZ selama tiga bulan. Biakan tunas pisang kemudian di iradiasi gamma pada 0, 20, 30, 40, 50, dan 60 Gy (Co60). Hasil analisis dengan CurveExpert 1.4 diketahui bahwa reduksi pertumbuhan tunas pisang sebesar 20-50% (LD20-50) berada pada kisaran 30.64-68.66 Gy. Hasil multiplikasi tunas setelah 6 bulan menunjukkan bahwa jumlah tunas dan daun terbanyak dihasilkan oleh eksplan yang tidak di iradiasi (0 Gy) dan jumlah nodul meristem dan tunas terendah dihasilkan oleh eksplan hasil iradiasi gamma 60 Gy. Skrining awal ketahanan tunas varian melalui teknik kultur ganda secara in vitro menunjukkan bahwa sebagian besar varian bersifat rentan terhadap cendawan Foc. Tunas varian pisang putatif agak tahan terhadap Foc berasal dari hasil iradiasi gamma 30 Gy. Kata kunci: dosis letal, iradiasi gamma, kultur ganda, pisang olahan.
Cultivation of Family Medicinal Plants using the Verticulture Method as Efforts to Use Narrow Yard Land in Rawamangun, East Jakarta Asharo, Rizal Koen; Lisanti, Elsa; Indrayanti, Reni; Adisyahputra; Pasaribu, Pinta Omas; Priambodo, Rizky; Rizkawati, Vina; Yulia Irnidayanti
Jurnal Pemberdayaan Masyarakat Madani (JPMM) Vol. 5 No. 1 (2021): Jurnal Pemberdayaan Masyarakat Madani (JPMM) (DOAJ & SINTA 3 Indexed)
Publisher : Fakultas Ekonomi dan Bisnis Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/10.21009/JPMM.005.1.05

Abstract

The area of RW 01 Rawamangun, Pulogadung District, East Jakarta does not have an allocation of open land, so there is a minimum of green yards. The technology introduced in this community service activity is the cultivation of family medicinal plants in yards using the verticulture method. The purpose of this community service activity is to convey information about the cultivation of family medicinal plants through lectures, discussions, and direct practice of cultivating family medicinal plants in yards using the verticulture method. Evaluation of participants' knowledge improvement was carried out by pre- and post test after counseling and cultivation practices. Skills participants are carried out when evaluating the practice of cultivating medicinal plants in their yards. The results of the activity showed an increase in basic knowledge regarding the types, benefits, and techniques of cultivating family medicinal plants from technology. Family medicinal plant service activity is classified as successful and beneficial, due to increased knowledge more than 60 in average test scoring. The skills evaluation showed that some residents were able to plant and maintain family medicinal plants on a narrow plot of land using the proper verticulture method. The results of the cultivation of these medicinal plants, apart from being one of the real actions of plant greening, can also be in the form of powder products which can then be consumed as a body health measure or sold.
In Vitro Medium-Term Storage of Banana Cultivar ‘Barangan’ Using Alginate-Encapsulation Technique Indrayanti, Reni; Wulandari, Nadia Fitria; Sutanto, Agus; Asharo, Rizal Koen; Adisyahputra
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.603-612

Abstract

In vitro medium-term storage using the alginate-encapsulation technique is essential for conserving plant genetic resources, preserving vegetatively propagated species by controlling the growth and development of explants, and maintaining plant biodiversity. The study aimed to obtain the optimum combination medium for the encapsulation of bananas and evaluate the viability of the alginate-encapsulated explants after storage. In vitro, medium- term storage of nodule-like meristem of banana cultivar ‘Barangan’ was performed using sodium alginate, paclobutrazol (PBZ), and Murashige and Skoog (MS) salt. This research consists of 3 stages: (1) Initiation and multiplication of in vitro shoots and nodule-like meristems; (2) In vitro medium-term storage of banana by encapsulation technique; (3) Regeneration of nodule-like meristem explants after in vitro storage. This research showed that the banana's number of nodule-like meristem was optimum on media supplemented with thidiazuron (TDZ) 0.22 mgL-1 and indole 3-acetic acid (IAA) 1.75 mgL-1. Encapsulation explant of nodules-like meristems using 3% sodium alginate in full and half-strength MS salt medium supplemented with PBZ 2.5 mgL-1 and incubated in liquid MS medium was able to store nodule-like meristem for six months. The color of the explants remains green, and the capsule is not damaged. Sub-culture of nodule-like meristem after storage in MS salt medium containing TDZ 0.22 mgL-1 and IAA 1.75 mgL-1 showed that nodules-like meristems could regenerate to form new shoots and nodule-like meristem three months after sub-cultured. This research concludes that encapsulated nodule-like meristem was recorded until six months of storage. PBZ was a necessary retardant in minimizing the growth during storage.
Isolation of Endophytic Bacteria from Melon Root and Evaluation of Their Antagonistic Activity Against Acidovorax citrulli Novitasari, Ayu; Desnurvia, Riza; Indrayanti, Reni
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1212-1222

Abstract

Endophytic bacteria are a potential biocontrol agent to control plant diseases. Controlling plant disease using bactericides has negative impacts, including the death of other organisms on plants. This research aimed to identify potential endophytic bacteria from melon roots for biocontrol against Acidovorax citrulli, the pathogen causing Bacterial Fruit Blotch (BLB) disease in the Cucurbitaceae family. A total of 11 endophytic bacteria were isolated from healthy melon roots. The isolates exhibited similar colony morphology with white, circular, convex elevation. Physiology and biochemistry tests revealed 8 isolates as Gram-negative and catalase-positive, while the remaining 3 were identified as Gram-positive and catalase-negative. A fluorescence test on King's B media indicated that the Gram-negative isolates could belong to the Pseudomonas genus. This study also confirmed Acidovorax sp. cultures isolated from infected melon plants many years ago as A. citrulli. Assessment of pathogenicity in the melon plants showed that A. citrulli isolate N2 was the most pathogenic. Based on the in vitro inhibition test, all the Gram-negative isolates formed inhibition zones ranging from 1.94-4.41 mm, suggesting their potential to inhibit the growth of A. citrulli. The EB6 isolate exhibited the highest inhibition zone at 4.41 mm ±0.28. five of the eight isolates tested (EA1, EB1, EB3, EB4, and EB6) did not exhibit any Hypersensitive Response (HR) reaction in the tobacco leaves, thus can potentially be used as a biocontrol agent against BLB disease in melon plants. Further studies are required to determine the species identity of the isolates and explore their application as biocontrol agents.
INDUKSI MUTASI PADA PISANG (Musa sp. - ABB) cv. KEPOK DENGAN IRADIASI GAMMA SECARA IN VITRO Masykuroh, Luthfia; Adisyahputra, Adisyahputra; Indrayanti, Reni
Bioma Vol. 12 No. 1 (2016): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (534.258 KB) | DOI: 10.21009/Bioma12(1).3

Abstract

Banana (Musa sp. - ABB) cv. Kepok is one of type banana processed that have a very potential commodities fruit developed to support food survival. The purpose of this study was to knowing the effect of gamma irradiation on the growth of banana plants cv. Kepok in vitro. This study was conducted in October 2014 – October 2015 in Plant Tissue Culture Laboratory, Biological – Science UNJ. The methods used was experiment with fully randomized design. Factors that tested was 6 gamma irradiation doses (0, 20, 30, 40, 50, 60 Gy) with 10 repetition. Observation of phenotypic generate diverse characters on the growth of the number of shoots and leaves. Gamma irradiation dose of 50 Gy is doses most inhibits the growth of character. Mutations that occur in banana plantlets cv. Kepok generated by the treatment doses gamma irradiation induced mutation is random.
PERKECAMBAHAN 4 AKSESI JEWAWUT (Setaria italica (L.) P. Beauv) PADA KONDISI CEKAMAN KEKERINGAN ARTIFISIAL Mapikasari, Septiani; Adisyahputra, Adisyahputra; Indrayanti, Reni
Bioma Vol. 13 No. 1 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.192 KB) | DOI: 10.21009/Bioma13(1).6

Abstract

Developing of jewawut cultivation as an alternative source of carbohydrates is one of the efforts to prevent food insecurity. Drought conditions and the availability of drought-tolerant seeds became one of the problems in the development of jewawut cultivation. The purpose of these experiments were to evaluate jewawut response to drought stress simulations at germination phases and to obtain accessions tolerant to drought stress. Drought stress is performed indirectly (PEG 6000 selective media). The research was done in Laboratory of Physiology of Faculty of Mathematic and Science, UNJ from February until July 2017. The experiments were done with a completely randomized design. Parameters of germination were analyzed with Anova test and continued by Duncan Multiple Range Test (DMRT). Lethal doses of PEG reducing 50% of germination (LD50) were 23,25%, with the quadratic equation y = 1,12-2,72x. The results base on germination phase, Buru merah as drought tolerance accessions, Polman merah and Polman kuning as medium tolerance accession, and Buru kuning as susceptible accessions.
UJI KETAHANAN NILAM TERHADAP Synchytrium pogostemonis PENYEBAB PENYAKIT BUDOK DAN POTENSI PENGENDALIANNYA DENGAN PESTISIDA NABATI Yuliyanti, Tri; Hartati, Sri Yuni; Indrayanti, Reni
Bioma Vol. 13 No. 2 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.166 KB) | DOI: 10.21009/Bioma13(2).5

Abstract

Nilam (Pogostemon cablin Benth.) merupakan tanaman semak penghasil minyak atsiri yang memiliki nilai ekonomi yang tinggi. Budidaya tanaman nilam memiliki kendala yaitu adanya serangan kapang Synchytrium pogostemonis penyebab penyakit budok. Tujuan dari penelitian ini adalah untuk mengetahui ketahanan 3 varietas nilam terhadap penyakit budok, dan mengetahui efektivitas dari formula tunggal dan campuran minyak mimba dan seraiwangi terhadap kejadian penyakit budok dan pengaruhnya pada pertumbuhan tanaman. Penelitian ini terdiri dari dua percobaan: 1) Efektivitas metode infeksi dan uji ketahanan tiga varietas nilam terhadap penyakit budok di rumah kaca, dan 2) Uji efektivitas minyak atsiri formula tunggal maupun campuran mimba dan seraiwangi sebagai pestisida nabati terhadap penyakit budok. Hasil percobaan efektivitas metode infeksi menujukkan bahwa metode perendaman akar tanaman dalam inokulum budok selama satu jam lebih efektif menginfeksi penyakit budok dibandingkan dengan metode penyiraman ke media tanam. Hasil uji ketahanan nilam varietas Sidikalang, Patchoulina 1 dan Patchoulina 2 menunjukkan bahwa varietas Patchoulina 1 lebih rentan terhadap penyakit budok karena menimbulkan keterjadian penyakit (KeP) dan keparahan penyakit (KP) hingga 20%, dengan karekter pertumbuhan tinggi tanaman yang lebih rendah dibandingkan varietas lain yang diuji. Sedangkan diantara minyak atsiri yang diujikan, formula minyak atsiri campuran (mimba dan seraiwangi) konsentrasi 0.5% lebih efektif dalam mengurangi penyakit budok, namun efektivitasnya masih lebih rendah dibandingkan dengan perlakuan fungisida benomil 0,3% dan bubur bordo 0,1%. Aplikasi formula minyak atsiri secara signifikan tidak berpengaruh negatif terhadap pertumbuhan nilam, sehingga formula campuran (mimba dan seraiwangi) 0,5% dapat digunakan untuk mengontrol penyakit budok pada tanaman nilam.
MULTIPLICATION AND ACCLIMATIZATION OF BANANA VARIANT CV. AMPYANG (Musa acuminata, AAA) PUTATIVE RESISTANCE TO FUSARIUM WILT Indrayanti, Reni; Yanti, Fitri; Adisyahputra, Adisyahputra; Dinarti, D; Sudarsono, Sudarsono
Bioma Vol. 14 No. 1 (2018): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.564 KB) | DOI: 10.21009/Bioma14(1).3

Abstract

Abstract Plants resistance to Fusarium wilt can be developed by mutation breeding and in vitro selection techniques. The objectives of this research were to evaluated growth and development of banana plantlets cv. Ampyang (Musa acuminata, AAA) resistance to Fusarium wilt. The plant material used in this study was 9 (nine) code clones banana cv. Ampyang result from mutations induced by gamma irradiation (30, 45, and 50 Gy) and in vitro - in vivo selection, a collection of Plant Tissue Culture Laboratory Department of Biology UNJ. Plantlets multiplied for four months on Murashige and Skoog supplemented with2.25 mgL-1 Benzyl amino purine and 0.22 mgL-1 Thydiazuron and 0.175 mgL-1 Indole 3 acetyc-acid . Acclimatization and evaluation of plantlet growth and development in a greenhouse showed that percentage of survival rate of plantlet were ranging from 42.9 – 100%. There was phenotypic variation among those plantlet investigated in quantitative and qualitative character. Plantlet regenerated from clone A regenerated from gamma irradiation 30 Gy and after in vitro selection of Foc from isolate Medan showed significantly the highest number of leave and fresh weight. The less number of roots length and other phenotypic character were produced from clone B, D and F. All of the regenerated plantlets were successfully transferred into soil and they would be used to evaluate the existence of variants in a greenhouse. Keywords: phenotypic variation, induce mutation and in vitro selection, shoot multiplication, Foc isolates from Medan
Isolation and Characterization of Pathogenic Microbes Origin in Strawberry (Fragaria sp.) Based on Koch's Postulates Asharo, Rizal Koen; Indrayanti, Reni; Damayanti, Aldira Putri; Putri, Hilda Arsyah Eka; Nabilah, Saskia; Pasaribu, Pinta Omas
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 2 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i2.269

Abstract

Strawberry (Fragaria sp.) is a horticultural plant originating from the Americas. Strawberries have a distinctive sweet and refreshing taste, and contain lots of vitamins and antioxidants that are useful for body health. But strawberries are also easily damaged. This damage can be caused by spoilage microorganisms such as bacteria and fungi that can grow if conditions permit such as the presence of appropriate temperature and humidity conditions. In this experiment, aims to isolate pathogenic microorganisms that cause disease in symptomatic strawberries and prove that these pathogens actually cause disease using Koch's Postulates method and then descriptive analysis is carried out. From the experimental results, it was found that soft rot disease in strawberries was characterized by rotten fruit parts that looked wet, slightly brownish in color, slimy, there were fine white threads and emitted a foul odor with a percentage of IP (disease occurrence) of 100% and KP (disease severity) of 92.66% where based on the score the damage was the highest.