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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Zuriat Jurnal AgroBiogen Buletin Penelitian Tanaman Rempah dan Obat Indonesian Journal of Science and Education Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pemberdayaan Masyarakat Madani (JPMM) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Biosfer: Jurnal Pendidikan Biologi Jurnal SOLMA Jurnal Biologi Tropis Bioma : Jurnal Biologi Indonesia Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Buletin Penelitian Tanaman Rempah dan Obat Mitra Mahajana: Jurnal Pengabdian Masyarakat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Berita Biologi

Reni Indrayanti

Universitas Negeri Jakarta

Author-ID : 507041

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Other

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UJI KETAHANAN NILAM TERHADAP Synchytrium pogostemonis PENYEBAB PENYAKIT BUDOK DAN POTENSI PENGENDALIANNYA DENGAN PESTISIDA NABATI Yuliyanti, Tri; Hartati, Sri Yuni; Indrayanti, Reni
Bioma Vol. 13 No. 2 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Nilam (Pogostemon cablin Benth.) merupakan tanaman semak penghasil minyak atsiri yang memiliki nilai ekonomi yang tinggi. Budidaya tanaman nilam memiliki kendala yaitu adanya serangan kapang Synchytrium pogostemonis penyebab penyakit budok. Tujuan dari penelitian ini adalah untuk mengetahui ketahanan 3 varietas nilam terhadap penyakit budok, dan mengetahui efektivitas dari formula tunggal dan campuran minyak mimba dan seraiwangi terhadap kejadian penyakit budok dan pengaruhnya pada pertumbuhan tanaman. Penelitian ini terdiri dari dua percobaan: 1) Efektivitas metode infeksi dan uji ketahanan tiga varietas nilam terhadap penyakit budok di rumah kaca, dan 2) Uji efektivitas minyak atsiri formula tunggal maupun campuran mimba dan seraiwangi sebagai pestisida nabati terhadap penyakit budok. Hasil percobaan efektivitas metode infeksi menujukkan bahwa metode perendaman akar tanaman dalam inokulum budok selama satu jam lebih efektif menginfeksi penyakit budok dibandingkan dengan metode penyiraman ke media tanam. Hasil uji ketahanan nilam varietas Sidikalang, Patchoulina 1 dan Patchoulina 2 menunjukkan bahwa varietas Patchoulina 1 lebih rentan terhadap penyakit budok karena menimbulkan keterjadian penyakit (KeP) dan keparahan penyakit (KP) hingga 20%, dengan karekter pertumbuhan tinggi tanaman yang lebih rendah dibandingkan varietas lain yang diuji. Sedangkan diantara minyak atsiri yang diujikan, formula minyak atsiri campuran (mimba dan seraiwangi) konsentrasi 0.5% lebih efektif dalam mengurangi penyakit budok, namun efektivitasnya masih lebih rendah dibandingkan dengan perlakuan fungisida benomil 0,3% dan bubur bordo 0,1%. Aplikasi formula minyak atsiri secara signifikan tidak berpengaruh negatif terhadap pertumbuhan nilam, sehingga formula campuran (mimba dan seraiwangi) 0,5% dapat digunakan untuk mengontrol penyakit budok pada tanaman nilam.

MULTIPLICATION AND ACCLIMATIZATION OF BANANA VARIANT CV. AMPYANG (Musa acuminata, AAA) PUTATIVE RESISTANCE TO FUSARIUM WILT Indrayanti, Reni; Yanti, Fitri; Adisyahputra, Adisyahputra; Dinarti, D; Sudarsono, Sudarsono
Bioma Vol. 14 No. 1 (2018): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Abstract Plants resistance to Fusarium wilt can be developed by mutation breeding and in vitro selection techniques. The objectives of this research were to evaluated growth and development of banana plantlets cv. Ampyang (Musa acuminata, AAA) resistance to Fusarium wilt. The plant material used in this study was 9 (nine) code clones banana cv. Ampyang result from mutations induced by gamma irradiation (30, 45, and 50 Gy) and in vitro - in vivo selection, a collection of Plant Tissue Culture Laboratory Department of Biology UNJ. Plantlets multiplied for four months on Murashige and Skoog supplemented with2.25 mgL-1 Benzyl amino purine and 0.22 mgL-1 Thydiazuron and 0.175 mgL-1 Indole 3 acetyc-acid . Acclimatization and evaluation of plantlet growth and development in a greenhouse showed that percentage of survival rate of plantlet were ranging from 42.9 â€“ 100%. There was phenotypic variation among those plantlet investigated in quantitative and qualitative character. Plantlet regenerated from clone A regenerated from gamma irradiation 30 Gy and after in vitro selection of Foc from isolate Medan showed significantly the highest number of leave and fresh weight. The less number of roots length and other phenotypic character were produced from clone B, D and F. All of the regenerated plantlets were successfully transferred into soil and they would be used to evaluate the existence of variants in a greenhouse. Keywords: phenotypic variation, induce mutation and in vitro selection, shoot multiplication, Foc isolates from Medan

Correlation between integrated science process skills, and ability to read comprehension to scientific literacy in biology teachers students Handayani, Gina; Adisyahputra, A; Indrayanti, Reni
Biosfer: Jurnal Pendidikan Biologi Vol. 11 No. 1 (2018): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Scientific literacy is define as the capacity of use scientific knowledge in order to understand and help make decision about the natural world and the changes made it through human activity. Teachers are a major factor in the success of learning, therefore it is necessary to test the scientific literacy skill of biology teacher students. This study aims to find out science literacy of biology teacher students and the corellation between of integrated science process skill, and reading comprehension skill of biology teacher students to their scientific literacy skill. The method for this research used the survey method with correlational studies. The sample of this research is 81 students of biology teacher students of Jakarta State University. The results shows that the scientific literacy of biology education students is destitute. It is shown from the average score of their scientific literacy skill which is 45.58. This study shows that correlation coefficient obtained is 0,457 which means that there is correlation between integrated science process skill with scientific literacy skill. This study also shows that there is correlation between reading comprehension skill with with scientific literacy skill which correlation coefficient obtained is 0,214.

Isolation and Characterization of Pathogenic Microbes Origin in Strawberry (Fragaria sp.) Based on Koch's Postulates Asharo, Rizal Koen; Indrayanti, Reni; Damayanti, Aldira Putri; Putri, Hilda Arsyah Eka; Nabilah, Saskia; Pasaribu, Pinta Omas
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 2 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i2.269

Strawberry (Fragaria sp.) is a horticultural plant originating from the Americas. Strawberries have a distinctive sweet and refreshing taste, and contain lots of vitamins and antioxidants that are useful for body health. But strawberries are also easily damaged. This damage can be caused by spoilage microorganisms such as bacteria and fungi that can grow if conditions permit such as the presence of appropriate temperature and humidity conditions. In this experiment, aims to isolate pathogenic microorganisms that cause disease in symptomatic strawberries and prove that these pathogens actually cause disease using Koch's Postulates method and then descriptive analysis is carried out. From the experimental results, it was found that soft rot disease in strawberries was characterized by rotten fruit parts that looked wet, slightly brownish in color, slimy, there were fine white threads and emitted a foul odor with a percentage of IP (disease occurrence) of 100% and KP (disease severity) of 92.66% where based on the score the damage was the highest.

Pemberdayaan Masyarakat melalui Budidaya dan Pengolahan Sayuran Microgreen di Desa Cisaat, Kecamatan Ciater, Kabupaten Subang Pasaribu, Pinta Omas; Indrayanti, Reni; Adisyahputra, Adisyahputra; Asharo, Rizal Koen; Rizkawati, Vina; Achmad, Farhana Faridah; Febrian, Reyno Ahmad; Utari, Raysita
Jurnal SOLMA Vol. 14 No. 2 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i2.17210

Background: Cisaat Village, located in Ciater District, Subang Regency, has significant agricultural potential. Most of its residents rely on agriculture as their primary source of livelihood. However, efforts to diversify agriculture and optimize land use in Cisaat Village have not been fully maximized. Most farmers still depend on conventional farming systems, which face challenges such as seasonal dependency, high production costs, and limited access to more efficient agricultural innovations. The purpose of comminity is introduce the public to the cultivation and processing of microgreens as a food source to boost the household economics in Cisaat Village, Ciater District, Subang Regency. Methods: The community service activity was carried out through socialization in the form of lectures, discussions, and demonstrations or hands-on practice on microgreen cultivation techniques and benefits. The evaluation was conducted using a pre-test before the activity and a post-test afterward to measure participants' improvement in understanding. The pre-test and post-test data were statistically analyzed using the Dependent T-test to determine the significance of differences before and after the activity. Results: Participants demonstrated high enthusiasm and were actively engaged throughout the activity. The T-test results showed a significant improvement in understanding, with the average pre-test score of 46.44 increasing sharply to 86.04 in the post-test (p < 0.05). This indicates that the applied method has proven to be effective. Conclusions: This socialization, combined with live demonstrations, proved helpful in introducing participants and boosting their interest in growing microgreens, both to meet household vegetable needs and as an additional income opportunity.

Effectiveness of Gibberellin Concentration (GA3) for The Growth and Propagation of Plant Cuttings Stevia Rebaudiana Pasaribu, Pinta Omas; Saputro, Sigit; Indrayanti, Reni; Adisayhputra, Adisayhputra
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7605

Stevia (Stevia rebaudiana) is a perennial semi-herbaceous plant whose leaves can be used as a sweetener for food and beverages. Stevia sweetener has low calories so its use is not bad for health. Propagation of Stevia by stem cutting using the top is a simple, fast, and easy propagation technique. This study aimed to obtain the optimum concentration of GA3 and the correct propagation method for Stevia cuttings for lowland areas. The research was conducted at the Green House, Biology Study Program FMIPA UNJ. The method used was an experimental method with a completely randomized design, consisting of 3 experiments, namely the provision of GA3 with a dose of 10 ppm, 30 ppm, and 50 ppm. Qualitative data were processed descriptively, quantitative data were analyzed based on ANOVA. The results showed that the use of Gibberellins with a concentration of 10 ppm and the maintenance of Stevia cuttings in clear plastic containers were more effective in the development of an average stem length of 19.18 ± 2.40, a mean plant length of 23.62 ± 2.34 and an average number of roots 14, 33 ± 2.32.

Propagation of Lily Arumsari using Generic Media through In Vitro Culture Kurniati, Ridho; Khairatunnisa, Fauziah; Indrayanti, Reni
Jurnal Hortikultura Indonesia (JHI) Vol. 11 No. 2 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.2.140-148

Perbanyakan lili secara in vitro umumnya menggunakan media Murashige dan Skoog (MS) dengan penambahan zat pengatur. Salah satu perhatian penting dalam produksi benih lili untuk skala masal dan produksi benih lili untuk industri adalah diperlukan media yang murah, mudah dan mampu menekan biaya produksi. Tujuan penelitian adalah mendapatkan media generik yang mudah, murah dan efisien untuk menggantikan media MS dan menurunkan biaya perbanyakan lili in vitro. Media yang digunakan adalah pupuk daun komersial dengan merek dagang Vitagrow, Growmore, Gibril, dan G-60. Umbi lili varietas Arumsari digunakan sebagai sumber eksplan perbanyakan secara in vitro. Percobaan menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal yaitu jenis media kultur generik. Setiap perlakuan media terdiri dari 3 ulangan, dengan 20 unit satuan percobaan tiap ulangan dan setiap unit terdiri dari 5 buah umbi. Parameter yang diamati meliputi jumlah daun, panjang daun, panjang dan jumlah akar dan persentase tumbuh umbi. Media Growmore merupakan media terbaik untuk menghasilkan jumlah daun terbanyak (50.67 daun), panjang daun terpanjang (1.6 cm), panjang akar serta (0.31 cm) persentase tumbuh tertinggi (100%). Jumlah akar terbanyak diperoleh pada media G-60.

Isolation and Characterization of Pathogenic Mold Causing Potato Tuber Rot Disease Asharo, Rizal Koen; Indrayanti, Reni; Amala, Azizatul; Raihan, Eldrian Daffa; Tampanguma, Raymond Rayhand; Putri, Hilda Arsyah Eka; Pasaribu, Pinta Omas; Wardana, Nurul Assyifa
Jurnal Penelitian Pendidikan IPA Vol 10 No 4 (2024): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i4.6355

The potato plant (Solanum tuberosum L.) is a perennial crop that contains high amounts of carbohydrates, minerals, and vitamins in its tubers, making it a carbohydrate-rich alternative food to rice or corn. One of the diseases that often appear on potato plants is potato rot caused by pathogenic molds. This study aims to isolate and characterize pathogenic fungi that cause blight on potato tubers based on Koch's Postulates. Potato pathogenic molds were isolated from potato tubers that had been rotted, then the molds were grown on PDA and purified twice. The purified isolates were then inoculated onto 30 healthy potato tubers and incubated for 7 days. Healthy potato tubers experienced the same symptoms as potato tuber rot. The isolated pathogenic fungi were then characterized so that the pathogenic fungi of Phytophthora infestans were obtained. In this study, Koch's Postulate method was successfully applied to isolate and characterize the pathogenic fungi that cause potato tuber rot. The pathogenic fungi caused a potato tuber rot disease incidence value of 100% with a disease severity value of 60.7%. The two factors that determine the disease incidence and disease severity values are internal factors (genes and traits of the pathogen) and external factors (environment).

Radiosensitivitas Pisang cv. Ampyang dan Potensi Penggunaan Iradiasi Gamma untuk Induksi Varian Indrayanti, Reni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 2 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Banana is commonly propagated vegetatively by suckers since most of edible banana are triploid, male sterile andparthenocarpic, use of conventional breeding for banana improvement is diffi cult. Mutation induction and in vitro techniqueare alternative tools for banana improvement. The objectives of this research were (1) to determine radiosensitivity of bananacv. Ampyang against gamma irradiation, and (2) to evaluate performance of plantlets regenerated from gamma irradiatedexplants of banana cv. Ampyang. Explants of in vitro grown shoots were exposed to gamma irradiation at 0, 20, 25, 30, 35,40, 45, and 50 Gy to determine their radiosensitivity. Growth and development of regenerated plantlets were recorded after10 months of proliferation and regeneration periods. The CurveExpert ver. 1.4 analysis results indicated that lethal doses ofirradiation reducing 20% to 50% of shoot growth (LD20-50) were 51.07 - 64.54 Gy. All regenerated plantlets from irradiatedexplants produced less numbers of roots, and some of regenerated plantlets, showed signifi cantly less plantlet fresh weightand height than the control one. Plantlets regenerated from explants irradiated with 25, 40, 50 Gy have longer leaves than thecontrol. The regenerated plantlets from gamma irradiation treatments were successfully transferred into soil and they wouldbe used to evaluate existence of variants among regenerated banana plantlets.Keywords: induced mutation, gamma irradiation, lethal dose (LD20-50)

INDUKSI POLIPLOIDI TANAMAN TELANG (Clitoria ternatea L.) DENGAN KOLKISIN Fransiska; Husni, Ali; Indrayanti, Reni
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.2513

Tanaman telang dapat dimanfaatkan sebagai alternatif pakan ternak karena kandungan nutrisinya. Produktivitas tanaman telang dapat ditingkatkan dengan menginduksi tanaman poliploidi dengan kolkisin. Tanaman poliploidi mempunyai ukuran fenotipik yang besar daripada diploidnya yang berpengaruh terhadap produktivitas tanaman tersebut. Tujuan dari penelitian ini adalah mengetahui pengaruh jenis telang dan lama perendaman kolkisin terhadap kecambah, karakter fenotipik dan tingkat ploidi tanaman. Penelitian ini dilaksanakan di Balai Besar Pengujian Standar Instrumen Bioteknologi dan Sumber Daya Genetik Pertanian (BSIP Biogen). Penelitian ini menggunakan Rancangan Acak Lengkap dengan duafaktor yaitu jenis telang (putih dan biru) dan lama perendaman kolkisin (0, 24, dan 48 jam. Data dianalisis secara statistik dengan ANAVA pada taraf nyata 5%. Telang biru dengan perendaman kolkisin selama 24 jam menghasilkan panjang daun, panjang stomata dan jumlah kloroplas tertinggi. Telang putih yang diberikan perlakuan perendaman kolkisin selama 24 jam menghasilkan tinggi kecambah dan lebar kotiledon yang paling tinggi dan perendaman kolkisin selama 48 jam menghasilkan karakter lebar daun, panjang dan lebar mahkota bunga terbesar. Telang biru dan putih dengan perlakuan perendaman kolkisin selama 24 dan 48 jam menghasilkan tanaman miksoploid (diploid dan tetraploid).

Co-Authors A.A. Ketut Agung Cahyawan W Abd. Rasyid Syamsuri Abdul Wahid Abdul Wahid Achmad, Farhana Faridah Adisayhputra, Adisayhputra Adisyahputra Adisyahputra Adisyahputra Adisyahputra Adisyahputra Adisyahputra, Adisyahputra Adisyahputra, A AGUNG SEDAYU Agus Sutanto Ali Husni Amala, Azizatul Amanda, Debby Viola Amanda, Debby Viola Asep Ali Asep Ali Asep Ali, Asep Asharo, Rizak Koen Asiani, Nia Asiani, Nia Bambang Sukmadi Bambang Sukmadi Bedah Rupaedah Bedah Rupaedah Bedah Rupaedah, Bedah Damayanti, Aldira Putri Debby Viola Amanda Debby Viola Amanda Desnurvia, Riza Dinarti, D Dono Wahyuno Dwi Eldina Dwi Ningsih Susilowati Elsa Lisanti Ervan Nurkholis Febrian, Reyno Ahmad Firmansyah, Taufik Firmansyah, Taufik Fitri Yanti Fransiska Handayani, Gina Ifa Manzila Khairatunnisa, Fauziah Mahmud Sugianto Mahmud Sugianto Mahmud Sugianto, Mahmud Mapikasari, Septiani Masykuroh, Luthfia Mieke Miarsyah Mieke Miarsyah Nabilah, Saskia NFN Sukamto Nia Asiani Nia Asiani Novi Listiana NOVITASARI, Ayu Nurhajati A. Mattjik Nurhajati A. Mattjik Pinta Omas Pasaribu Putri, Hilda Arsyah Eka Rafika Yuniawati Rahayu Rahayu Raihan, Eldrian Daffa Ridho Kurniati Rizal Koen Asharo Rizal Koen Asharo Rizkawati, Vina Rizky Priambodo Saputro, Sigit Setiawan, Asep S Siti Fatimah Sri Yuni Hartati Sudarsono Sudarsono Sukmadi, Bambang Sukmadi, Bambang Tampanguma, Raymond Rayhand Taufik Firmansyah Taufiq Firmansyah Tri Puji Priyatno Tri Yuliyanti Utari, Raysita Vina Rizkawati Wardana, Nurul Assyifa Wulandari, Nadia Fitria YULIA IRNIDAYANTI

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