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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Widyariset BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Biodidaktika : Jurnal Biologi dan Pembelajarannya ANNALES BOGORIENSES JURNAL SELULOSA Widyariset Indonesian Journal of Applied Environmental Studies Jurnal Sylva Lestari Journal of Tropical Biodiversity Jurnal Kefarmasian Indonesia Annales Bogorienses
Endang Sukara
Universitas Nasional
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Journal : BERITA BIOLOGI

 1 
VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3242.558 KB) | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
KEANEKARAGAMAN HAYATI (EMAS HIJAU) ALTERNATIF BAGI INDONESIA KELUAR DARI KRISIS MULTIDIMENSI Sukara, Endang
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (823.202 KB) | DOI: 10.14203/beritabiologi.v7i6.861

Abstract

Krisis ekonomi yang terjadi di Indonesia terutama bersumber dari dalam negeri kita sendiri.Pemerintah orde baru, terutama sejak era konglomerasi (1987-1994), terlalu mengabaikan berkembangnya kesenjangan.Pertumbuhan ekonomi ternyata hanya sebuah fatamorgana (Mubyarto,2000). Krisis ekonomi 1997-1998 dan keadaan politik yang tidak menentu, mengakibatkan nilai mata uang rupiah dalam tempo yang amat singkat, menurun drastis. Indonesia pun langsung terpuruk. Kegiatan ekonomi tersendat karena sebagian besarnya sangat tergantung kepada bahan baku impor. Banyak perusahaan yang gulung tikar dan banyak buruh terpaksa di PHK. Jumlah penganggur dan jumlah penduduk miskin meningkat dengan tajam.
TIGA RATUS TAHUN LINNEAUS - SUATU PENGANTAR Sukara, Endang
BERITA BIOLOGI Vol 8, No 4(a) (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.633 KB) | DOI: 10.14203/beritabiologi.v8i4(a).1533

Abstract

Three hundred years ago, a Sweden expert, Carl von Linne had laid down a very basic and strong foundation in naming all the living organisms on earth.Supposedly, without the brilliant effort of Carl von Linn6, nowadays, there may have any chaos among people around the world in naming all the living creatures, because everyone would have given the name to those organisms, due to their own sense; thus a kind species may have thousands of name. If this is happen, then all the information regarding the species (including its benefit to mankind) may have no meaning at all. All people would have spoken about a species differently, without realize that they actually regarding about the same species.The center of references such as Herbarium Bogoriense and Museum Zoologicum Bogoriense may be not existed without the work and service of Carolus Linnaeus. No one nowadays arguing the great service of Carolus Linnaeus in naming of each living organisms based on binomial nomenclature.
KEANEKARAGAMAN HAYATI (EMAS HIJAU) ALTERNATIF BAGI INDONESIA KELUAR DARI KRISIS MULTIDIMENSI Endang Sukara
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i6.861

Abstract

Krisis ekonomi yang terjadi di Indonesia terutama bersumber dari dalam negeri kita sendiri.Pemerintah orde baru, terutama sejak era konglomerasi (1987-1994), terlalu mengabaikan berkembangnya kesenjangan.Pertumbuhan ekonomi ternyata hanya sebuah fatamorgana (Mubyarto,2000). Krisis ekonomi 1997-1998 dan keadaan politik yang tidak menentu, mengakibatkan nilai mata uang rupiah dalam tempo yang amat singkat, menurun drastis. Indonesia pun langsung terpuruk. Kegiatan ekonomi tersendat karena sebagian besarnya sangat tergantung kepada bahan baku impor. Banyak perusahaan yang gulung tikar dan banyak buruh terpaksa di PHK. Jumlah penganggur dan jumlah penduduk miskin meningkat dengan tajam.
TIGA RATUS TAHUN LINNEAUS - SUATU PENGANTAR Endang Sukara
BERITA BIOLOGI Vol 8, No 4(a) (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i4(a).1533

Abstract

Three hundred years ago, a Sweden expert, Carl von Linne had laid down a very basic and strong foundation in naming all the living organisms on earth.Supposedly, without the brilliant effort of Carl von Linn6, nowadays, there may have any chaos among people around the world in naming all the living creatures, because everyone would have given the name to those organisms, due to their own sense; thus a kind species may have thousands of name. If this is happen, then all the information regarding the species (including its benefit to mankind) may have no meaning at all. All people would have spoken about a species differently, without realize that they actually regarding about the same species.The center of references such as Herbarium Bogoriense and Museum Zoologicum Bogoriense may be not existed without the work and service of Carolus Linnaeus. No one nowadays arguing the great service of Carolus Linnaeus in naming of each living organisms based on binomial nomenclature.
VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR Sulistiani Sulistiani; Wibowo Mangunwardoyo; Abinawanto Abinawanto; Endang Sukara; Achmad Dinoto; Andi Salamah
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
Co-Authors Abd. Rasyid Syamsuri Abinawanto Abinawanto Achmad Dinoto Achmad Dinoto Andi Hartono Andi Salamah Atit Kanti Atit Kanti Bernadeta Ayu Widyaningrum Dede Hermawan Dolly Priatna Ernawati Sinaga Evi Triana Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Febia Rini Hanies Ambarsari Hastuti, Ratih Dwi Herman Suparman Simanjuntak Jajang Sutiawan KATSUHIKO ANDO Kusumah, Sukma Surya Latifah K Darusman latifah K Darusman Latifah K. Darusman Lisdayanti, Puspita Lukman Hakim Miranti Nurindah Sari MISA OTOGURO Nampiah Sukarno NITA ROSALINDA PRAYITNO Noverita Nuniek Ina Ratnaningtyas Nuraeni Ekowati Purity Sabila Ajiningrum Puspita Lisdayanti PUSPITA LISDIYANTI Puspita Lisdiyanti Puspita Lisdiyanti Ragamustari, Safendrri K. Ragamustari, Safendrri Komara Rohmatussolihat - ROHMATUSSOLIHAT ROHMATUSSOLIHAT Rohmatussolihat Rohmatussolihat Ruth Meliawati Ruth Melliawati Ruth Melliawati Shanti Ratnakomala SHANTI RATNAKOMALA Solly Aryza Sri Lestari Subkhan Subkhan Sukma Surya Kusumah Sulistiani ., Sulistiani Sulistiani Sulistiani Sulistiani Sulistiani, Sulistiani Sutiawan, Jajang Trisanti Anindyawati Trisanti Anindyawati, Trisanti WIBOWO MANGUNWARDOYO Wibowo Mangunwardoyo Widyaningrum, Bernadeta Ayu Widyastuti, Anisa Y. Purwanto Yantyati Widyastuti Yantyati Widyastuti Yantyati Widyastuti YOPI YOPI Yopi Yopi YULIN LESTARI Yulneriwarni Yulneriwarni