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All Journal Science and Technology Indonesia Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Pengabdian Pada Masyarakat Indonesia (JPPMI) Jurnal Pengabdian Masyarakat Panacea Jurnal Pengabdian Fakultas Pertanian Universitas Lampung
WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH
Department of Biology, Faculty of Mathematics and Natural Sciences, Lampung University, Jalan Prof. Soemantri Brojonegoro No. 1 Bandar Lampung 35145, Indonesia
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Journal : Science and Technology Indonesia

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Activity of Mangrove-Derived Fusarium equiseti 20CB07RF Extract Against Clinical, Antibacterial-Resistant Pseudomonas aeruginosa Bahri, Syaiful; Setiawan, Wawan Abdullah; Setiawan, Fendi; Lutfiah, Rosyidatul; Juliasih, Ni Luh Gede Ratna; Ambarwati, Yuli; Ahmadi, Peni; Arai, Masayoshi; Hendri, John; Hadi, Sutopo; Setiawan, Andi
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.594-604

Abstract

Endophytic fungi originating from mangroves are potential sources of secondary metabolites with varying bioactivities. This research explores the bioactive metabolites produced by endophytes derived from mangrove plants. Endophytic fungi were collected from various parts of several mangrove plants (roots, stems, and leaves, as well as the surrounding mud). A total of 17 endophytics fungi were obtained. The isolates were derived from the leaves (1 isolate), stems (8 isolates), roots (5 isolates), and surrounding mud (3 isolates). A single fungal colony was cultured using solid-state fermentation for 14 days. The fermented fungal biomass was extracted using ethyl acetate (EtOAc) and evaluated for its antibacterial activity against clinical pathogenic bacteria. In the preliminary screening, the EtOAc extract of the CB07RF1 isolate exhibited notable growth-inhibitory effects against Pseudomonas aeruginosa. The isolate was verified by molecular identification using a study of the rDNA internal transcribed spacer (ITS) sequence, revealed that isolate CB07RF1 was very similar to Fusarium equiseti (99% similarity). Isolate 20CB07RF1, obtained by solid-state fermentation using a rice medium indicated as peptide compound group, and featured active components that exhibited potent growth inhibitory activity against Pseudomonas aeruginosa at a concentration of 12.5 mg/mL. This study demonstrates, for the first time, that Fusarium equiseti extracts grown in a rice medium contain antimicrobial compounds that can inhibit the growth of P. aeruginosa, an important clinical pathogen known for its antibacterial resistance. These findings accent mangrove endophytic fungi as important sources of bioactive compounds and will advance related research in the fields of biotechnology, pharmacology, and life sciences.
Novel Micrococcus unila to Produce Glucosamine by Solid-state Fermentation of Shrimp Shell Waste Setiawan, Wawan Abdullah; Setiawan, Andi; Salsabila, Nafila Khansa; Widyastuti, Widyastuti; Laila, Aspita; Juliasih, Ni Luh Gede Ratna; Irawan, Bambang; Ahmadi, Peni; Apriliana, Ety; Arai, Masayoshi; Hendri, John
Science and Technology Indonesia Vol. 9 No. 4 (2024): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.4.779-789

Abstract

This study aimed to assess glucosamine production through enzymatic activity, utilizing actinomycetes sourced from shrimp shell waste (SSW) in a solid-state fermentation (SSF) process. A total of 16 actinomycetes underwent chitinase activity screening, and the strain exhibiting the highest chitinolytic index was chosen for subsequent morphological and phylogenetic analyses. High Performance Liquid Chromatography (HPLC) was employed to analyze glucosamine produced from the bioconversion of SSW via SSF. Optimal conditions for glucosamine production were determined by varying time, pH, and temperature. Isolate 18D36-A2 showed the highest chitinolytic index of 1.02 in the 32-mm clean zone. Phylogenetic analysis revealed 97% similarity to the genus Micrococcus, identifying it as a novel Micrococcus unila strain 18D36-A2 and deposited in GenBank. This isolate effectively converted shrimp shells. The findings showcase the bioconversion of SSW to glucosamine through SSF using the Micrococcus unila 18D36-A2. Furthermore, this study establishes a foundation for future research on environmentally friendly and sustainable designs for glucosamine production.
Co-Authors -, Mohamad Kanedi Ahmadi, Peni Ambarwati, Yuli Andi Setiawan Arai, Masayoshi Ardian Ardian Aspita Laila Damayanti, Rizka Dwi Emantis Rosa Erwin Yuliadi Ety Apriliana Fitri Yelli Gina Dania Pratami, Gina Dania Handayani, Kusuma - John Hendri Juliasih, Ni Luh Gede Ratna Kukuh Setiawan Kusuma Handayani, Kusuma Lutfiah, Rosyidatul Muhammad Kamal Muhammad Syamsul Hadi, Muhammad Syamsul Mumtazah, Dzul Fithria Nuning Nurcahyani Oktariana, Putri Rosa, Emantis Salsabila, Nafila Khansa Sanjaya, Purba Setiawan, Fendi Sutopo Hadi SYAIFUL BAHRI Tengku Riza Zarzani N Tundjung Tripeni Handayani, Tundjung Tripeni Widyastuti Widyastuti