Article Per Year (5 Year)
p-Index From 2020 - 2025
1.554
P-Index
This Author published in this journals
All Journal bionature Majalah Obat Tradisional Sari Pediatri Majalah Kedokteran Andalas Mattawang: Jurnal Pengabdian Masyarakat Narra J Berita Kedokteran Masyarakat Bionature
Yusuf Yenni
Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

Published : 16 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 16 Documents
Search

 1   2 
Voluntary Counselling and Testing (VCT) HIV and Sexually Transmitted Diseases (STD) Detection among People Living in the Bontomangape Health Center Service Area, Galesong, Takalar: Pos Voluntary Councelling and Testing (VCT) HIV dan Deteksi Infeksi Menular Seksual (IMS) pada masyarakat di Wilayah Kerja Puskesmas Bontomangape, Galesong, Takalar Yusuf, Yenni; Amiruddin, Dianawaty; Mubin, Risna Halim
Mattawang: Jurnal Pengabdian Masyarakat Vol. 5 No. 4 (2024)
Publisher : Yayasan Ahmar Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35877/454RI.mattawang3279

Abstract

The incidence of HIV in the Bontomangape Galesong Health Center work area has raised a concern for Hasanuddin University (UNHAS). Partnering with the health center, UNHAS team carried out Voluntary Counseling and Testing (VCT) posts to educate the community and to conduct early detection of HIV in the health center work area. The activities in the VCT post included the playing of educational videos, the distribution of HIV andsexually transmitted diseases (STD) educational leaflets, counseling to undertake tests for family members of people with HIV, counseling to do tests on the people at risk, rapid antigen HIV-Syphilis examination, and post-screening counseling. The results and outputs produced in program were (1) HIV and STD educational videos and leaflets, (2) Publication in the online media, and (3) Increased public knowledge and awareness regarding the dangers and risk factors of HIV, and the importance of early detection of HIV and STI. Abstrak Tingkat kejadian HIV di daerah pesisir termasuk di wilayah kerja Puskesmas Bontomangape Galesong menjadi perhatian Universitas Hasanuddin (UNHAS). Bermitra dengan pihak puskesmas, tim UNHAS melaksanakan pos Voluntary Counselling and Testing (VCT) untuk edukasi dan skrining HIV di wilayah kerja puskesmas. Kegiatan pos VCT termasuk pemutaran video edukasi dan penyebaran leaflet HIV dan Infeksi menular seksual (IMS), konseling untuk melakukan tes bagi para anggota keluarga ODHIV, konseling test pada masyarakat umum yang beresiko, pemeriksaan rapid antigen HIV-Sifilis, dan konseling pasca skrining. Luaran yang dihasilkan pada kegiatan ini adalah (1) Video dan leaflet edukasi HIV dan IMS, (2) Publikasi pada media online, dan (3) meningkatnya pengetahuan dan kesadaran masyarakat mengenai bahaya dan faktor risiko HIV, serta pentingnya deteksi dini HIV dan IMS.
Evaluation of an E. coli-expressed spike protein-based in-house ELISA system for assessment of antibody responses after COVID-19 infection and vaccination Nurisyah, Sitti; Iyori, Mitsuhiro; Hasyim, Ammar A.; Amru, Khaeriah; Itani, Kei; Nakamura, Kurumi; Zainal, Kartika H.; Halik, Handayani; Djaharuddin, Irawaty; Bukhari, Agussalim; Asih, Puji BS.; Syafruddin, Din; Yoshida, Shigeto; Idris, Irfan; Yusuf, Yenni
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1250

Abstract

Evaluating long-term immunity after COVID-19 infection and vaccination is critical for managing potential outbreaks. The aim of this study was to develop a cost-effective in-house enzyme-linked immunosorbent assay (ELISA) based on Escherichia coli-expressed SARS-CoV-2 spike protein (E-S1) for antibody detection and to evaluate its performance. The system was validated by comparing the in-house ELISA results with those obtained using a commercial ELISA with HEK293-expressed spike protein (H-S1). Recombinant SARS-CoV-2 spike protein was produced in E. coli, purified, and validated for antigenicity via ELISA. Indirect ELISAs with both E-S1 and H-S1 antigens were performed on 386 serum samples from COVID-19 survivors, vaccinated individuals, and pre-pandemic controls collected at different time points. The E-S1 ELISA showed a statistically significant but weak correlation with H-S1 ELISA across all samples (r=0.205; p=0.0001). Stronger correlations were observed among vaccinated individuals with prior infection on day 90 (r=0.6017; p<0.001) and in naïve vaccine recipients on day 30 (r=0.5361; p=0.0003). Pre-pandemic sera from a rural population in Sumba Island exhibited high background reactivity in E-S1 ELISA, likely due to anti-E. coli antibodies, while urban pre-pandemic sera from Jakarta showed a stronger correlation with H-S1 ELISA. This suggests potential regional or immune background differences influencing assay performance. Although E-S1 retained antigenic properties, its diagnostic utility is limited by non-specific reactivity and reduced sensitivity compared to H-S1. In conclusion, E. coli expression systems may not be ideal for producing spike protein-based ELISA antigens specific to SARS-CoV-2. Alternative expression systems, such as human or baculovirus, could enhance diagnostic accuracy and specificity for COVID-19 antibody detection.
Penggunaan Bakteri Bacillus spp untuk Pengendalian Jentik Nyamuk Anopheles spp (Application of Bacillus spp as Microbial Larvicides to Control Anopheles Larvae) Yenni Yusuf
Bionature Vol. 10 No. 2 (2009): Oktober 2009
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Based on the high incidence and fatality of malaria, a comprehensive management to control the disease, including Anopheles larval control to remove the transmission path, is urgently required. Chemical insecticides used for this purpose is claimed to be less environmentally safe and potentially increase the mosquito resistance. Thus, there is a need to use alternative insecticide. Some studies found that Bacillus sphaericus dan Bacillus thuringiensis israeli could effectively kill Anopheles larvae and has a low potency to increase resistency. Besides that, it is environmentally safe and does not affect non-target organisms. Therefore, the use of Bacillus spp as larvicides could be undertaken in malaria control programme especially in endemic areas.
Analisis Hubungan Stunting dan Infeksi Kecacingan pada Anak di Daerah Pesisir Makassar Putri, Sri Hardiyanti; Salekede, Setia Budi; Juliaty, Aidah; Yusuf, Yenni; Maulani, Destya; Abdullah, Arwini Avissa
Sari Pediatri Vol 27, No 2 (2025)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp27.2.2025.108-12

Abstract

Latar belakang. Stunting masih menjadi masalah kesehatan di Indonesia. Infeksi kecacingan pada seorang anak dapat berdampak terhadap status gizi yang jika diabaikan akan bersifat kronik dan menjadi faktor risiko stunting.Tujuan. Menilai pengaruh infeksi kecacingan terhadap kejadian stunting pada anak di daerah pesisir.Metode. Penelitian ini dilakukan di Desa Untia Makassar Sulawesi Selatan. Pengambilan data dilakukan secara potong lintang, yakni pemeriksaan antropometri anak usia 6 bulan-5 tahun untuk menentukan stunting atau tidak, kemudian dilakukan pemeriksaan sampel feses untuk menilai adanya infeksi kecacinganHasil. Terdapat 100 anak yang direkrut dalam penelitian ini, 21 anak (21%) menderita stunting dan 79 anak (79%) tidak stunting. Dari pemeriksaan sampel feses masing-masing ditemukan satu anak menderita kecacingan pada kelompok stunting (4,8%) dan tidak stunting (1,2%). Berdasarkan analisa statistik tidak ditemukan perbedaan bermakna diantara kedua kelompok (p=0,378).Kesimpulan. Tidak ditemukan pengaruh yang signifikan antara kejadian infeksi kecacingan pada anak stunting di daerah pesisir.
Evaluasi Rasio Netrofil Limfosit (RNL) untuk diagnosis COVID-19 pada Pasien di RS Tadjuddin Chalid, Makassar, Indonesia Yenni Yusuf; Sitti Nurisyah; Ammar Abdurrahman Hasyim; Khaeriah Amru; Atifatul Qalbi Kadir; Azizah Nurul Mutia; Muhammad Naufal Zuhair; Kartika Paramita
Bionature Vol. 22 No. 2 (2021): Oktober 2021
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The neutrophil lymphocyte ratio (NLR) is one of the variables related to the severity of COVID-19 disease. Therefore, we evaluated the value of NLR in COVID-19 patients and its relation to the symptoms and severity of COVID-19 at Tadjuddin Chalid Hospital, Makassar. We recruited patients visiting Tadjuddin Chalid Hospital Makassar who were willing to take part in the study in April to August 2021. Demographic, clinical and laboratory data were recorded through interviews and medical records. The severity of the disease is classified according to the guidelines from the association of Indonesian pulmonary doctors, Indonesian internal medicine doctors, Indonesian pediatricians, anesthesiologists and intensive care doctors, and the Indonesian cardiologist association. NLR data was obtained by calculating the ratio between neutrophil levels and lymphocyte levels in the blood. A total of 130 patients took part in the study, between 19-72 years old. There were 77 subjects (59.2%) with mild symptoms, 28 (21.6%) with moderate symptoms, and 25 (19.2%) with severe symptoms. There were 89 subjects with NLR <3.5 (68.5%) and 41 (10.8%) with NLR > 3.5. NLR values in patients with severe and moderate disease were significantly different from NLR in patients with mild disease (p =0.0002 dan p<0.0001, respectively). However, no significant difference was found in NLR between patients who neither have cough nor fever with those who had one or both of those symptoms. The presence of clinical or radiological symptoms of pneumonia accompanied by an NLR > 3.5 can be used as an indicator for a suspected moderate or severe COVID-19 diagnosis.
Tinjauan Molekuler dan Epidemiologi Mutasi pada Virus SARS-CoV-2 Hartono; Yenni Yusuf
Bionature Vol. 22 No. 1 (2021): April 2021
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Virus SARS-CoV-2 yang merupakan penyebab pandemi COVID-19 sejakakhir tahun 2019 telah mengalami banyak mutasi yang memunculkan beberapavariants of concern (VOC) dengan penularan, virulensi, dan kemampuanmenghindari sistem imun yang lebih tinggi daripada varian awal (wild-type). Hinggakini terdapat empat varian yang termasuk dalam VOC virus tersebut yaitu varianalfa, beta, gamma, dan delta. Peningkatan transmisi dan virulensi pada VOC tersebutdikaitkan dengan perubahan mutasi pada protein spike yang merupakan strukturdari virus yang berperan dalam pengikatan dengan sel host. Pada artikel ini kamimelakukan literature review mengenai VOC dari virus SARS-CoV-2 terkait mutasiyang terjadi dan dampaknya pada proses pengikatan virus. Untuk mendapatkanpemahaman terkait dampak dari mutasi di berbagai varian tersebut, kami jugamereview struktur dari protein spike dan proses entry virus ke dalam sel host.
Co-Authors . Yuliastuti, . Abdullah, Arwini Avissa Aidah Juliaty, Aidah Amiruddin, Dianawaty Ammar Abdurrahman Hasyim Amru, Khaeriah Ari Andini Junaedi Asih, Puji BS. Asri, Rangga Meidianto Atifatul Qalbi Kadir Azizah Nurul Mutia Banjar, Muhammad Fadhil Bukhari, Agussalim Din Syafruddin Djaharuddin, Irawaty Elim, Diany Go, Rivaldo Halik, Handayani Hartono Hartono Hartono Hartono Hartono Hasyim, Ammar A. Hasyim, Ammar Abdurrahman IRFAN IDRIS Itani, Kei Iyori, Mitsuhiro Kadir, Atifatul Qalbi Kaharuddin, Kania Meliani Kartika Paramita Maulani, Destya Mubin, Risna Halim Muhammad Naufal Zuhair Mutia, Azizah Nurul Mutmainnah Nakamura, Kurumi Nur Azizah Fadhilah Nurisyah, Sitti Parenden, Martrisna Dara Karnia Putri, Sri Hardiyanti Salekede, Setia Budi Saputra, Mesakh Diki Sitti Nurisyah Sitti Wahyuni Sumastuti Regina, Sumastuti Veithzal Rivai Zainal Yoshida, Shigeto Zainal, Kartika H. Zuhair, Muhammad Naufal