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All Journal bionature Majalah Obat Tradisional Sari Pediatri Majalah Kedokteran Andalas Mattawang: Jurnal Pengabdian Masyarakat Narra J Berita Kedokteran Masyarakat Bionature
Yusuf Yenni
Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Evaluation of an E. coli-expressed spike protein-based in-house ELISA system for assessment of antibody responses after COVID-19 infection and vaccination Nurisyah, Sitti; Iyori, Mitsuhiro; Hasyim, Ammar A.; Amru, Khaeriah; Itani, Kei; Nakamura, Kurumi; Zainal, Kartika H.; Halik, Handayani; Djaharuddin, Irawaty; Bukhari, Agussalim; Asih, Puji BS.; Syafruddin, Din; Yoshida, Shigeto; Idris, Irfan; Yusuf, Yenni
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1250

Abstract

Evaluating long-term immunity after COVID-19 infection and vaccination is critical for managing potential outbreaks. The aim of this study was to develop a cost-effective in-house enzyme-linked immunosorbent assay (ELISA) based on Escherichia coli-expressed SARS-CoV-2 spike protein (E-S1) for antibody detection and to evaluate its performance. The system was validated by comparing the in-house ELISA results with those obtained using a commercial ELISA with HEK293-expressed spike protein (H-S1). Recombinant SARS-CoV-2 spike protein was produced in E. coli, purified, and validated for antigenicity via ELISA. Indirect ELISAs with both E-S1 and H-S1 antigens were performed on 386 serum samples from COVID-19 survivors, vaccinated individuals, and pre-pandemic controls collected at different time points. The E-S1 ELISA showed a statistically significant but weak correlation with H-S1 ELISA across all samples (r=0.205; p=0.0001). Stronger correlations were observed among vaccinated individuals with prior infection on day 90 (r=0.6017; p<0.001) and in naïve vaccine recipients on day 30 (r=0.5361; p=0.0003). Pre-pandemic sera from a rural population in Sumba Island exhibited high background reactivity in E-S1 ELISA, likely due to anti-E. coli antibodies, while urban pre-pandemic sera from Jakarta showed a stronger correlation with H-S1 ELISA. This suggests potential regional or immune background differences influencing assay performance. Although E-S1 retained antigenic properties, its diagnostic utility is limited by non-specific reactivity and reduced sensitivity compared to H-S1. In conclusion, E. coli expression systems may not be ideal for producing spike protein-based ELISA antigens specific to SARS-CoV-2. Alternative expression systems, such as human or baculovirus, could enhance diagnostic accuracy and specificity for COVID-19 antibody detection.
Penggunaan Bakteri Bacillus spp untuk Pengendalian Jentik Nyamuk Anopheles spp (Application of Bacillus spp as Microbial Larvicides to Control Anopheles Larvae) Yenni Yusuf
Bionature Vol. 10 No. 2 (2009): Oktober 2009
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

Based on the high incidence and fatality of malaria, a comprehensive management to control the disease, including Anopheles larval control to remove the transmission path, is urgently required. Chemical insecticides used for this purpose is claimed to be less environmentally safe and potentially increase the mosquito resistance. Thus, there is a need to use alternative insecticide. Some studies found that Bacillus sphaericus dan Bacillus thuringiensis israeli could effectively kill Anopheles larvae and has a low potency to increase resistency. Besides that, it is environmentally safe and does not affect non-target organisms. Therefore, the use of Bacillus spp as larvicides could be undertaken in malaria control programme especially in endemic areas.
Analisis Hubungan Stunting dan Infeksi Kecacingan pada Anak di Daerah Pesisir Makassar Putri, Sri Hardiyanti; Salekede, Setia Budi; Juliaty, Aidah; Yusuf, Yenni; Maulani, Destya; Abdullah, Arwini Avissa
Sari Pediatri Vol 27, No 2 (2025)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp27.2.2025.108-12

Abstract

Latar belakang. Stunting masih menjadi masalah kesehatan di Indonesia. Infeksi kecacingan pada seorang anak dapat berdampak terhadap status gizi yang jika diabaikan akan bersifat kronik dan menjadi faktor risiko stunting.Tujuan. Menilai pengaruh infeksi kecacingan terhadap kejadian stunting pada anak di daerah pesisir.Metode. Penelitian ini dilakukan di Desa Untia Makassar Sulawesi Selatan. Pengambilan data dilakukan secara potong lintang, yakni pemeriksaan antropometri anak usia 6 bulan-5 tahun untuk menentukan stunting atau tidak, kemudian dilakukan pemeriksaan sampel feses untuk menilai adanya infeksi kecacinganHasil. Terdapat 100 anak yang direkrut dalam penelitian ini, 21 anak (21%) menderita stunting dan 79 anak (79%) tidak stunting. Dari pemeriksaan sampel feses masing-masing ditemukan satu anak menderita kecacingan pada kelompok stunting (4,8%) dan tidak stunting (1,2%). Berdasarkan analisa statistik tidak ditemukan perbedaan bermakna diantara kedua kelompok (p=0,378).Kesimpulan. Tidak ditemukan pengaruh yang signifikan antara kejadian infeksi kecacingan pada anak stunting di daerah pesisir.
Adverse reactions following COVID-19 immunization in children of elementary school age Yusuf, Yenni; Junaedi, Ari Andini; Mutmainnah; Fadhilah, Nur Azizah; Wahyuni, Sitti
BKM Public Health and Community Medicine Vol 40 No 02 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bkm.v40i02.9712

Abstract

Purpose: Due to the low rate of parent acceptance of vaccines, this study was conducted to evaluate post-vaccination adverse events among elementary school students. Methods: This survey took place in the Manggala Subdistrict of Makassar City. The study was conducted from September to October 2022 by distributing a paper-based questionnaire regarding the COVID-19 vaccines. Results: Of 129 respondents, the age range was 7-12 years. Sixty-five percent of respondents received the first vaccine shot in January or February, with a 4-week interval of the second dose among 46.5% of children. The vaccine administered was CoronaVac from Sinovac. The most common adverse events were injection pain (45-48%) and fever (15-17%). The other side effects were itching, swelling, fever, and shortness of breath. Around 17-18 % of respondents did not have any adverse reactions. There is no increase in the risk for younger children (years 1-3) compared with older children (years 4-6). Conclusion: The children reported only mild adverse events such as injection pain, weakness, and fever. The CoronaVac has minimal side effects among elementary school children.
Co-Authors . Yuliastuti, . Abdullah, Arwini Avissa Aidah Juliaty, Aidah Amiruddin, Dianawaty Amru, Khaeriah Ari Andini Junaedi Asih, Puji BS. Asri, Rangga Meidianto Banjar, Muhammad Fadhil Bukhari, Agussalim Din Syafruddin Djaharuddin, Irawaty Elim, Diany Fachry Abda El Rahman Fadhilah, Nur Azizah Go, Rivaldo Halik, Handayani Hartono Hartono Hartono Hasyim, Ammar A. Hasyim, Ammar Abdurrahman IRFAN IDRIS Itani, Kei Iyori, Mitsuhiro Kadir, Atifatul Qalbi Kaharuddin, Kania Meliani Maulani, Destya Mubin, Risna Halim Mutia, Azizah Nurul Nakamura, Kurumi Nurisyah, Sitti Parenden, Martrisna Dara Karnia Putri, Sri Hardiyanti Salekede, Setia Budi Saputra, Mesakh Diki Sitti Wahyuni Sumastuti Regina, Sumastuti Veithzal Rivai Zainal Yoshida, Shigeto Zainal, Kartika H. Zuhair, Muhammad Naufal