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Potential Characterization and Identification of Indigenous Rhizobacteria Species of Ultisol Soil to Support the Growth of Several Superior Upland Rice Varieties Sapto Nugroho Hadi; Ida Widiyawati; Syaeful Anwar
Jurnal Penelitian Pertanian Terapan Vol 21 No 3 (2021)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v21i3.2146

Abstract

TG4 and SR2 were isolates of indigenous bacteria from cassava roots from Banyumas Regency, Central Java. Both are local isolates from marginal lands that can be developed as biofertilizers. This study aimed to determine the potential characteristics of bacterial isolates TG4 and SR2 in supporting the growth of superior upland rice and determining species identity based on the molecular analysis of 16S rRNA. Bacterial isolates TG4 and SR2 were determined for their potency of Plant Growth Promoting Rhizobacteria (PGPR) by fixing N2, dissolving phosphate, and producing Indole Acetic acid (IAA). Bioassays were carried out on TG4 and SR2 isolates by application of bacterial isolates (B0 = control, B1 = TG4, B2 = isolates SR2, B3 = isolates TG4 and SR2) on superior upland rice (V1 = INPAGO UNSOED 1, V2 = UNSOED PARIMAS, V3 = INPAGO 8) in sterile ultisol soil. The F test was used for bioassay data analysis, and if there was a significant difference, it was further tested with Duncan's Multiple Range Test (DMRT) with an error rate of 5%. The identity of bacterial species TG4 and SR2 was obtained by analyzing 16S rRNA sequences and genetic relationships through phylogenetic trees. The results showed that the isolates of TG4 were phosphate solubilizing bacteria and producers of IAA, while the isolates of SR2 were nitrogen fixing, phosphate solubilizing, and IAA producers. The application of bacterial isolates TG4 and SR2 significantly affected root length wet and dry weight of upland rice plants, with the highest value obtained from using a consortium of TG4 and SR2 bacteria. Bacterial isolates TG4 were identified as Bacillus albus, while SR2 as B. Paramycoides. Bacterial isolates TG4 and SR2 can be used as biofertilizers to support superior upland rice growth.
PENERAPAN HIDROPONIK DAN PASCAPANEN SAYURAN PADA ORANGTUA SISWA SDN KARANGSALAM KABUPATEN BANYUMAS Ahmad Fauzi; Prita Sari Dewi; Wilis Cahyani; Sapto Nugroho Hadi
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 5 No. 1 (2021): Jurnal Panrita Abdi - Januari 2021
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v5i1.7788

Abstract

Abstract. Community service activities in the form of application of cultivation techniques and post-harvest processing of vegetable crop into healty and nutritious food products with the target of elemetary school students were proven to be able to increase: student’s interest, knowledge, and skills towards vegetable farming activities; consumption frequency of vegetable-based food; knowledge of various types of vegetables dan their benefits. The activitis need to be transmitted to the parents to solve problems: the habit of parents preparing instant foods that are minimal vegetable content for their children’s breakfast; the lack of parental knowledge in processing vegetabales to a variety of foods that are preferred by the children; limited knowledge about vegetables benefit for children growth and development; and house environment have not been used optimally for vegetable cutivation. The purpose of this activity were: training on vegetable cultivation techniques using two different hydroponic system (NFT and wick system); post-harvest knowledge transfer of vegetable into preferred foods for the children; knowledge transfer about the importance of vegetables for childern growth and development. The method implemented in this activity were making demonstration and plot (demplot) of NFT dan wick system; vegetable cultivation activities through direct practice; post-harvest knowledge transfer of vegetable into preferred foods for the children; transfer of knowledge about healthy food with balanced nutrition to support the growth and development of school-age children. As a result, all activities are going well with high target audience participation. Hydroponic demonstration plots are prepared to produce crops of kale, spinach, pakcoy up to four times. Through this activity, parents' knowledge and skills have increased in: cultivation of vegetable plants using hydroponic techniques; postharvest processing of vegetable products into foods that children like;  knowledge of the importance of the benefits of vegetables for children.     Abstrak. Kegiatan pengabdian kepada masyarakat berupa aplikasi budidaya dan pameran produk makanan berbasis sayuran dengan khalayak sasaran siswa sekolah dasar terbukti mampu meningkatkan ketertarikan, pengetahuan, dan keterampilan siswa terhadap kegiatan bertanam sayuran; frekuensi konsumsi makanan berbahan sayuran; pengetahuan ragam jenis sayur dan manfaatnya. Kegiatan perlu diterapkan lebih luas kepada orang tua siswa untuk mengatasi permasalahan kebiasaan orang tua menyiapkan makanan instan yang minim sayur untuk anaknya; minimnya pengetahuan orang tua dalam melakukan kegiatan pengolahan tanaman sayuran menjadi aneka makanan yang disukai anak-anak; terbatasnya pengetahuan pentingnya manfaat sayuran bagi anak usia sekolah, dan; belum termanfaatkannya lingkungan rumah untuk kegiatan bertanam sayuran. Tujuan kegiatan ini adalah memberikan pengetahuan dan keterampilan kepada orang tua siswa SDN Karangsalam melalui pelatihan teknik budidaya tanaman sayuran menggunakan teknik hidroponik NFT dan Wick system untuk pemanfaatan lahan pekarangan rumah; transfer pengetahuan pascapanen produk tanaman hasil panen menjadi produk makanan yang disukai anak-anak, dan; transfer pengetahuan pentingnya manfaat sayuran bagi pertumbahan dan perkembangan anak usia sekolah. Metode yang dilaksanakan dalam kegiatan pengabdian ini adalah metode partisipatif. Khalayak sasaran diajak berperan aktif dalam kegiatan pembuatan perangkat hidroponik tanaman sayuran; demplot hidroponik; kegiatan budidaya tanaman sayuran dengan praktik langsung; transfer pengetahuan pascapanen produk tanaman hasil panen menjadi produk makanan yang disukai anak-anak; transfer pengetahuan pentingnya manfaat sayuran bagi anak usia sekolah. Hasilnya, pengetahuan dan keterampilan orang tua meningkat dalam hal: budidaya tanaman sayuran menggunakan teknik hidroponik; pengolahan pascapanen produk sayuran menjadi makanan yang disukai anak-anak; dan pentingnya manfaat sayuran bagi anak-anak.
Penggunaan Mikrosatelit dan RAPD untuk Analisis Keragaman Genetik Kentang di Sentra Budidaya Kentang Jawa Tengah Sapto Nugroho Hadi
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 6 No. 2 (2022): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sejumlah klon kentang dibudidayakan di sentra budidaya kentang, Dataran Tinggi Dieng, Provinsi Jawa Tengah. Penelitian ini bertujuan untuk mengetahui (1) Nilai Persentase Lokus Polimorfisme (PLP), (2) Nilai Polymorphism Information Content (PIC), dan (3) Jarak genetik aksesi kentang berbasis penanda molekuler mikrosatelit dan Random Amplified Polymorphic DNA (RAPD). DNA genom diisolasi dari daun delapan klon kentang berbeda, kemudian diamplifikasi dengan primer oligonukleotida mikrosatelit (STM 3015, RGH 8, RGH POT 83/8, RGH STIO53) dan RAPD (OPP13, OPNO2). Pola pita DNA diberi skor dengan memberikan angka 1 untuk kemunculan dan 0 untuk ketidakhadiran. Hasil skoring digunakan untuk menyusun pohon filogenetik berdasarkan metode unweighted pair-group method with arithmetic (UPGMA). Hasil penelitian menunjukkan rata-rata PLP 93% dan nilai PIC untuk primer RGH SSR8, StI053, dan OPN02 bersifat informatif. Konstruksi pohon filogenetik menunjukkan dua kelompok utama dalam jarak genetik. Dua cluster utama dikelompokkan menjadi dua subkluster. Subkluster pertama terdiri atas Lapan, Kentang Ungu, Granola Lembang, dan Kentang Merah. Subkluster kedua terdiri atas NH1, MZ, Agria, dan Vega. 
Identification of Potential Biofertilizer and Bioremediator Bacteria from Upland Soil Based on 16s rDNA Sequence Analysis Hadi, Sapto Nugroho; Widiyawati, Ida; Fauzi, Ahmad; Dewi, Prita Sari; Ahadiyat, Yugi R.
PLANTA TROPIKA Vol 11, No 2 (2023)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v11i2.12744

Abstract

The long-term presence of synthetic pesticides on agricultural land can lead to a decline in soil fertility. Synthetic pesticides inhibit the activity of essential enzymes in the soil and suppress beneficial microbial populations for plants. One potential approach to mitigate the extent of contamination caused by synthetic pesticides involves the utilization of indigenous pesticide-resistant bacteria. Several upland soil bacteria from Banyumas Regency, Central Java Province, Indonesia, were successfully isolated from a previous study. The isolated bacteria have the potential to be developed as pesticide bio-remediators and biofertilizers. The bacterial isolates are expected to have characters that support plant growth through their ability to provide dissolved phosphate. However, the potential bacterial isolates need to be identified by molecular approaches. This study was conducted to identify bacterial isolates of GT2, SR1, SW1, and PA1 by 16S rDNA sequencing analysis. The results showed that isolate GT2 was placed within a group of reference strains of Bacillus proteolyticus, isolate SR1 was placed within a group of B. paramycoides, isolate SW1 was set within a group of B. albus, and isolate PA1 was placed within a group of Acidovorax delafieldii. The genetic distance of isolate GT2 and B. B. proteolyticus, isolate SR1 and B. paramycoides, isolate SW1 and B. albus were 0.0000 each, and isolate PA1 and A. delafieldii were 0.0061. 
EFISIENSI PUPUK NITROGEN DAN PERTUMBUHAN SORGUM PADA TANAH ULTISOL DENGAN PEMANFAATAN KOMPOS BAGLOG JAMUR Ahmad Fauzi; Wilis Cahyani; Ida Widiyawati; Sapto Nugroho Hadi
Jurnal Agrotek Tropika Vol 12, No 1 (2024): JURNAL AGROTEK TROPIKA VOL 12, Februari 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i1.6759

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Tanaman sorgum sebagai alternatif tanaman pangan cukup potensial dibudidayakan di lahan kering yang masih tersedia sangat luas di Indonesia. Permasalahan pada lahan kering seperti ultisol adalah kemasaman dan rendahnya ketersediaan hara. Pemupukan kimia khususnya nitrogen pada tanah ultisol dinilai memiliki efisiensi rendah. Sehingga diperlukan peningkatan efisiensi pemupukan nitrogen untuk meningkatkan produktivitas tanaman sorgum di tanah ultisol. Penelitian ini bertujuan untuk meningkatkan efisiensi pemupukan nitrogen melalui penambahan kompos limbah baglog jamur tiram pada budidaya sorgum di tanah ultisol. Penelitian dilakukan pada Maret sampai Oktober 2020 di area Kebun Benih Bojong Sari, Balai Benih Dinas Pertanian Provinsi Jawa Tengah. Rancangan Acak Kelompok digunakan dalam penelitian ini dengan mengujikan dosis urea (0%, 25%, 50%, 75% dan 100%) pada tiga dosis kompos limbah baglog (5, 10 dan 15 ton/ha). Sebanyak 3 kali ulangan dilakukan pada 12 kombinasi perlakuan, sehingga didapatkan 36 unit percobaan. Hasil pengamatan menunjukkan pemupukan urea mampu meningkatan pertumbuhan dan hasil tanaman sorgum di tanah ultisol. Pemberian kompos baglog mampu meningkatkan efisiensi pemupukan nitrogen. Efisiensi terbaik diperoleh pada dosis 25% (87 kg/ha) dengan kombinasi kompos baglog 15 ton/ha, yaitu sebesar 46,80%.
Cadmium (Cd) heavy metal reduction in liquid and soil using indigenous soil bacteria isolated from Cd-contaminated paddy fields Hadi, Sapto Nugroho; Haryanto, Totok Agung Dwi; Tamad, Tamad; Ahadiyat, Yugi R.
Journal of Degraded and Mining Lands Management Vol. 12 No. 5 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.125.8561

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The challenge in Indonesian agriculture is the contamination of cadmium (Cd) in rice fields. Cadmium contamination in rice fields is a serious environmental and health problem. Reducing Cd contamination from rice fields is essential. One way is through the use of indigenous microbes. The study aimed to obtain indigenous bacteria from Cd-contaminated rice fields and determine their ability to reduce Cd contamination in liquid and soil media. Soil samples were taken from six locations of Cd-contaminated rice fields in Banyumas Regency, Central Java, Indonesia. The population of soil bacteria was calculated. Selected isolates were tested for their tolerance and ability to reduce Cd. Potential isolates were identified based on their 16S rDNA sequences. The results showed that the most prominent Cd-tolerant bacteria were found in soil samples from Ajibarang, with 1.8 × 106 CFU/g of soil. The study obtained five isolates with high tolerance to Cd, up to 100 ppm, and was able to reduce Cd by 45.58% to 70.02% in liquid media and from 0.92 ppm to 0.02 ppm in soil media. All bacterial isolates are non-pathogenic to plants. Isolate GML2, SRU2, and KBS1 were from Priestia megaterium, JTL1 was from Neobacillus ginsengisoli, and PLK1 was from Gottfriedia sp. The isolates have the potential to be used to reduce Cd contamination and remediate rice fields contaminated with Cd.
ISOLASI BAKTERI LOKAL LAHAN MARGINAL DAN KARAKTERISASI BERDASARKAN LAJU PERTUMBUHAN PADA MEDIA MENGANDUNG BUPROFEZIN Hadi, Sapto Nugroho; Widiyawati, Ida; Dewi, Prita Sari
Agrin Vol 22, No 2 (2018): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.82 KB) | DOI: 10.20884/1.agrin.2018.22.2.465

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Tujuan dari penelitian ini adalah untuk mengisolasi bakteri lokal dari tanah marjinal dari KabupatenBanyumas dan untuk melihat tingkat pertumbuhannya pada media yang mengandung buprofezin 0 ppm, 5 ppm,10 ppm dan 15 ppm. Bakteri diisolasi dari empat sampel tanah dari tanah marginal Desa Srowot, Desa Karangrao,Desa Tanggeran, dan Desa Pagaralang di Kabupaten Banyumas. Populasi bakteri dan koloni makromorfologidiamati untuk menentukan koloni bakteri dominan. Bakteri dominan ditanam pada media NB selama 26 jam untukmelihat kurva pertumbuhan. Bakteri dengan kurva pertumbuhan terbaik kemudian diinkubasi pada media NB yangmengandung buprofezin 0 ppm, 5 ppm, 10 ppm, dan 15 ppm untuk melihat laju pertumbuhan selanjutnya. Variabelyang diamati adalah populasi bakteri tanah, karakter makromorfologi bakteri, laju pertumbuhan bakteri pada mediatanpa pestisida, dan laju pertumbuhan bakteri pada media mengandung buprofezin. Dalam penelitian ini, empatkoloni bakteri dominan, yaitu, SR2, KR1, TG4, dan PA11 diisolasi dari 26 koloni yang tumbuh pada media NAyang mengandung buprofezin 2 ppm. Pengamatan laju pertumbuhan pada media NB tanpa pestisida menunjukkankoloni SR2 memiliki laju pertumbuhan terbaik. Koloni SR2 yang ditumbuhkan pada media NB yang mengandungbuprofezin menunjukkan bahwa koloni dapat beradaptasi dan tumbuh pada konsentrasi buprofezin 5 ppm.Kata kunci: bakteri lahan marginal, Banyumas, buprofezinABSTRACTThe aim of this study is to isolate local bacteria of marginal land from Banyumas regency and to see it’sgrowth rate on media containing buprofezin 0 ppm, 5 ppm, 10 ppm and 15 ppm. Bacteria were isolated from foursoil samples from marginal land of Srowot Village, Karangrao Village, Tanggeran Village, and PagaralangVillage of Banyumas Regency. Bacterial populations and macromorphologic colonies were observed fordetermination of dominant bacterial colonies. The dominant bacteria were grown on NB media for 26 hours tosee the growth curve. Bacteria with the best growth curve then incubated on NB media containing buprofezin 0ppm, 5 ppm, 10 ppm, and 15 ppm to see further growth rate. The variables observed were the population of soilbacteria, the character of the macromorphology of the bacteria, the rate of bacterial growth in the media withoutpesticides, and the rate of bacterial growth in the media containing buprofezin. In this study, four dominantbacterial colonies, namely, SR2, KR1, TG4, and PA11 were isolated from 26 colonies grown on NA mediacontaining buprofezin 2 ppm. Observation of growth rate on NB media without pesticide showed colony of SR2has the best growth rate. Colony SR2 was growth on NB media containing buprofezin showed the colony can adaptand grow at 5 ppm buprofezin concentration.Keyword: Marginal Land Bacteria, Banyumas, Buprofezin
STRATEGI KLONING DAN EKSPRESI GEN var2csa PADA SISTEM Escherichia coli SEBAGAI LANGKAH PENDAHULUAN UNTUK EKSPRESI GEN PADA SISTEM EUKARIOT TANAMAN Hadi, Sapto Nugroho; Noviyanti, Rintis
Agrin Vol 19, No 1 (2015): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2015.19.1.351

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Penelitian ini bertujuan mengetahui apakah strategi yang digunakan dapat berhasil mengkloning danmengekspresikan sekuen DNA target (daerah DBL3x gen var2csa) pada inang E.coli. Strategi kloning dilakukanmelalui tahapan: perbanyakan sekuen DNA target melalui teknik PCR menggunakan primer yang didesain sendiri,peligasian sekuen DNA target pada vektor kloning pCR2.1-TOPO, dan memperbanyaknya pada inang E.coliTOP10. Strategi ekspresi dilakukan melalui tahapan: perbanyakan sekuen DNA target melalui teknik PCRmenggunakan primer yang mengandung situs pengenalan enzim restriksi endonuklease tipe II: NcoI dan XhoI,pemotongan dengan enzim NcoI dan XhoI, peligasian pada vektor ekspresi pET-28a (+), dan pengekspresian padainang E. coli BL21 (DE3) dengan induser isopropyl-1-thio-ß-D-galactoside (IPTG) 1 mM. Strategi kloningberhasil mendapatkan sekuen daerah DBL3x gen var2csa berukuran 948 pb. Strategi ekspresi berhasilmendapatkan protein DBL3x berukuran 38 kDa.Kata kunci: kloning, ekspresi, DBL3x, var2csa, Escherichia coliABSTRACTThe objective of this study is to see are the strategies approach that used successfully clone and expressDBL3x region of var2csa gene in E.coli host. The cloning strategies are amplifying DNA target through PCRtechnique using self design oligonucleotide primer, inserting into cloning vector pCR2.1-TOPO, copying inE.coli TOP10 host. The expression strategies are amplifying DNA target through PCR using self design primerwith specific site for restriction enzyme NcoI and XhoI, digesting with NcoI and XhoI, inserting into pET-28a (+)expression vector, and expressing in E. coli BL21 (DE3) host by adding isopropyl-1-thio-ß-D-galactoside (IPTG)1 mM as a inducer. This study successfully cloned DBL3x region of var2csa gene with 948 pb in size andsuccessfully expressed protein DBL3x with 38 kDa in size in E.coli system.Keywords: cloning, expression, DBL3x, var2csa, Escherichia coli
OPTIMASI DAN EFISIENSI TEKNIK ISOLASI RNA DAUN DAN AKAR KELAPA SAWIT (Elaeis guineensis Jacq) Sari, Dini Astika; Martiansyah, Irfan; Mukmin, Restu Prasetya; Hadi, Sapto Nugroho; Syahputra, Indra; Afandi, Dadang; Putranto, Riza Arief
Agrin Vol 23, No 2 (2019): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.431 KB) | DOI: 10.20884/1.agrin.2019.23.2.500

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Tanaman kelapa sawit memiliki kandungan polisakarida dan polifenol yang tinggi. Kontaminasi polisakarida dan polifenol menyebabkan sulitnya proses isolasi RNA dari jaringan tanaman kelapa sawit. Penelitian ini dilakukan untuk mengoptimasi beberapa protokol isolasi RNA tanaman kelapa sawit yang efektif dan efisien. Penelitian dilaksanakan selama tiga bulan, pada bulan Oktober-Desember 2018 di Laboratorium Biokimia dan Biologi Molekuler, Pusat Penelitian Bioteknologi dan Bioindustri Indonesia (PPBBI), Jalan Taman Kencana No. 1 Bogor 16128. Penelitian dilakukan dengan melaksanakan teknik isolasi RNA menggunakan tiga protokol, yaitu modifikasi Cetyl Trimethyl Ammonium Bromide (CTAB), kit isolasi RNA RNeasy Plant Mini Kit (Qiagen), dan kit isolasi NucleoSpin RNAPlant (Macherey-Nagel). Sampel yang digunakan adalah daun dan akar tanaman kelapa sawit berumur kurang dari tiga bulan dengan bobot 0,1 gram dan 2,5 gram yang disesuaikan untuk tiap protokol. Variabel yang diamati adalah konsentrasi (ng/µl), kemurnian (rasio A260/A280 dan A260/A230), dan pita RNA pada elektroforesis gel agarosa. Hasil penelitian menunjukkan, RNA total hasil isolasi protokol NucleoSpin RNAPlant (Macherey-Nagel) memiliki kualitas paling tinggi. Konsentrasi RNA total daun dan akar kelapa sawit yang didapatkan melalui protokol NucleoSpin RNAPlant (Macherey-Nagel) sebesar 338 ng/µl dan 184,4 ng/µl dengan rasio A260/A280 RNA total daun dan akar kelapa sawit sebesar 2,13 dan 2,18 serta rasio A260/A230 sebesar 2,09 dan 2,20. Hasil elektroforesis gel agarosa menunjukkan integritas yang bagus dari RNA total hasil isolasi RNeasy Plant Mini Kit (Qiagen) dan NucleoSpin RNAPlant (Macherey-Nagel), namun terdapat kontaminasi dan smear pada RNA total hasil isolasi CTAB modifikasi 1 dan 2. Kata kunci: kelapa sawit, isolasi RNA, spektrofotometer, elektroforesis gel agarosa
PENDAMPINGAN PEMBUATAN PUPUK DAN PESTISIDA ORGANIK GUNA MENDUKUNG USAHA BUDIDAYA STROBERI PADA KELOMPOK TANI SIDA URIP DESA SERANG KEC. KARANGREJA PURBALINGGA Herliana, Okti; Hadi, Sapto Nugroho; Cahyani, Wilis; Rostaman, Rostaman
Jurnal Abdi Insani Vol 11 No 3 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i3.1965

Abstract

Strawberries have various benefits for human health so they are widely loved by the public. Serang Village, Karangreja District, Purbalingga. This village is located on the slopes of Mount Slamet with an altitude of about 650 - 1,600 m dp is one of the centers of strawberry cultivation in Central Java, but since 2016 it has experienced a decline in production due to poor quality seeds, high fertilizer prices, declining soil fertility and the existence of pests and plant diseases. The purpose of this research-based community service activity is to implement the results of the research to solve the problems that exist in the Sida Urip farmer group, provide training in making organic fertilizers and pesticides, assist in the practice of making organic fertilizers and pesticides, improve skills in maintaining strawberry plants, so that it can increase strawberry production The method used in this activity is Rural Appraisal Participation, which is the active involvement of all elements of the target audience, the stages of the activity include: Survey, socialization of activities, training, practice, demonstration plot making and evaluation. The results of the activity showed that there was an increase in the knowledge and skills of farmer groups in making compost and biological agents to support environmentally friendly strawberry cultivation. The eco-friendly strawberry demonstration plot produces 38.69 kg/bed of strawberries and conventional strawberry cultivation land produces 37.45 kg/bed. The assistance carried out by the service team also increased enthusiasm and cohesiveness among members of the Sida Urip farmer group.