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All Journal METAMORFOSA Journal of Biological Sciences Jurnal MIPA Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Pendidikan Biologi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioedukatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bioedukasi: Jurnal Pendidikan Biologi Jurnal Sains dan Edukasi Sains Indigenous Biologi Proceeding Biology Education Conference Bioed : Jurnal Pendidikan Biologi BIOCHEPHY: Journal of Science Education Al-Khidmah Jurnal Pengabdian Masyarakat AGRIC Engagement: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia
Sri Kasmiyati
Universitas Kristen Satya Wacana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : AGRIC

 1 
DETEKSI CEKAMAN OKSIDATIF AKIBAT TOKSISITAS KROM PADASonchus oleraceus L. MELALUI PENENTUAN SPESIES OKSIGEN REAKTIF SECARA SPEKTROFOTOMETRI DAN HISTOKIMIA Sri Kasmiyati; - Sucahyo
Agric Vol. 26 No. 1 (2014)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2014.v26.i1.p85-98

Abstract

Increased production of reactive oxygen species or ROS is one of the common responses to a wide range of biotic and abiotic stresses. Increased production of ROS is outstripping endogenous antioxidant defense systems has been referred to as oxidative stress. Heavy metals are known to initiate ROS generation which is implicated as a oxidative stress. Cr is a toxic heavy metal that can generate ROS like H2O2 and O2 - which cause oxidative stress. In this study, chromium toxicity was studied to detect the oxidative stress on Sonchus oleraceus weed plants by the detection of superoxide anion and H2O2. Superoxide anion was detected by staining techniques with nitroblue tetrazolium (NBT) and hydrogen peroxide by Diaminobenzidine tetrahydrochloride (DAB) staining. Results indicated that the plants were grown in sand media generate the highest (0.89 A/g FW and 3.23 mol/g FW) than in soil media (0.23 A/g FW and 2.11 mol/g FW) superoxide anion (*O- 2) and H2O2 and soil containing textile sludge (0.18 A/g FW and 2.66 mol/g FW), respectively. At application of 10 mg Cr6+/L and 250 mg Cr3+/L, the production of *O- 2 and H2O2 in leaves of sonchus plants were significantly increased compared with the control plants. The highest production of H2O2 and *O- 2 were showed in the leaves of sonchus plants grown in sand media with Cr6+ application. In this study, either Cr3+ or Cr6+ caused oxidative stress in Sonchus oleraceus weed plants. The result also showed that sonchus plants esposed to toxic Cr can suffer from oxidative stress leading to reduction of its fresh and dry plants biomass. NBT and DAB in an appropriate probe and significant value for monitoring the formation of *O- 2 and H2O2 in plants.
SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI in vitro EKSTRAK HEKSANA-PETROLEUM ETER Artemisia cina Berg. ex Poljakov Elizabeth B.E Kristiani; Sri Kasmiyati; Maria Marina Herawati
Agric Vol. 27 No. 1 (2015)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2015.v27.i1.p30-37

Abstract

The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.
Efek Interaksi Sulfate (SO42-) dan Kromium (CrO42-) terhadap Pertumbuhan dan Akumulasi Cr6+ pada Tagetes erecta L. Sri Kasmiyati; Gabriella L. Mataradja
Agric Vol. 35 No. 1 (2023)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2023.v35.i1.p45-60

Abstract

Chromium is a heavy metal that is toxic, bioaccumulative, persistent, and cannot be decomposed in the environment. The Cr6+ ion is a chromium ion that has the highest toxicity among other chromium ions, so its presence in the environment is hazardous for plants, animals, and humans. Sulfur is an essential element for plants, whose presence can reduce Cr6+ concentrations in the terrestrial environment by inhibiting the uptake of Cr6+ by plants. Tagetes erecta is a bioremediation agent plant that can remediate land polluted with Cr6+ waste. In this research, we studied the growth of T. erecta in media polluted with Cr6+ and sulfate and its ability to accumulate of Cr6+. Several parameters observe in this experiment, i.e. growth parameters, namely plant height, number of leaves, number of flowers measured at the end of the study, dry weight, root length, and Cr6+ concentration estimated using the diphenylcarbasid method. The results showed that the plant height of T. erecta. was not significantly different, and sulfate addition did not affect plant height. The addition of sulfate affected the number of leaves and flowers, dry weight of root shoots, root length, and Cr6+ content in T. erecta. polluted with Cr6+. so that in these parameters, there was a significant difference in the addition of Cr6+ and sulfate on growth of T. erecta and its ability to Cr accumulation.
Co-Authors ,, Djohan Agna Sulis Krave Agustien Sri Noerwahju Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Anna Helena Sabandar Araujo, Otilda Fatima Ariyanto, Prasetyo Eko Betty Elok, Elizabeth Cahyaningrum, Desti Christian Cantika Glodia Debora Kristiani Rahardjo Desti Christian Cahyaningrum Dewi Prabawaningrum Djohan Djohan Djohan Djohan Djurubasa, Dani Karel Marthin Egarani, Grahita Rezhi Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Elizabeth Bety E.K Fanny Nurwahidah, Cantika Fathiya Ulfa Nurrasyida Fatima Araujo, Otilda Gabriella L. Mataradja Hapsari Sekar Widiantari, Olive Ie, Johanes Juan Yutama Putra Indrianasari, Putri Irfan Dwidja Priyambada, Irfan Dwidja Jokosulistyo Wartanto Juliana Sarioa Kachingwe, Baxter Hepburn Kapitarauw, Agnesya Giovani Putri Cendana Keliat, Natalia Rosa Kumala Dewi L. Hartanto Nugroho Lani, Rischa Karmila Margareta Prameswati Maria Marina Herawati Monica Mangudis, Vicharisti Nabila, Nabila Natalia Rosa Keliat Novellasari, Firdiana Penina Intansari Prasgi, Henokh Christian Pratama, Dimas Seno Bagus Priyayi, Desi Fajar Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri, A C. Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rintawati Sandradewi, Rintawati Risya Pramana Situmorang Rully Adi Nugroho Sabandar, Anna Helena Santosa S, Santosa Satria Dharmawan Kustia Dewa Seleiman, Mahmoud F. Setya Magdalena, Ajeng Shinta, Damaris Clara Siahaya, Esty Vicariat Sucahyo Sucahyo Sucahyo Susanti Pudji Hastuti Susanti Puji Hastuti Syuhuud Arumbinang Wajdi Tambunan, Roslinda Kristin Trisianna, Jessica Ayu Trisianna Vincentia Irene Meitiniarti Vini Kurnia Ramadhani Wahyu Hari Kristiyanto Widyaningrum, Indy Yohanes Martono Yolanda Halim, Vivian