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PENINGKATAN PENGETAHUAN DAN KETERAMPILAN KELOMPOK PENGOLAH DAN PEMASAR (POKLAHSAR) MINA LESTARI BATU LUMBANG DENPASAR MELALUI PEMBUATAN MINUMAN HERBAL DAUN JERUJU I Made Wisnu Adhi Putra; Putu Angga Wiradan; A.A.A. Putri Permatasari
J-ABDI: Jurnal Pengabdian kepada Masyarakat Vol. 4 No. 9: Februari 2025
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jabdi.v4i9.9607

Kelompok Pengolah dan Pemasar (Poklahsar) Mina Lestari merupakan kelompok yang dibentuk selama Pandemi COVID-19 di Ekowisata Mangrove Batu Lumbang, Denpasar, Bali. Kelompok ini mengembangkan Hasil Hutan Bukan Kayu (HHBK) yang berbasis hutan mangrove. Beberapa produk yang sudah dibuat meliputi: produk olahan makanan ringan, minuman tradisional, fashion, dan produk lulur. Salah satu produk yang menjadi unggulan Poklahsar sebagai mitra adalah simplisia daun Jeruju. Selama menjalankan usahanya, mitra mengalami beberapa masalah diantaranya adalah: mitra masih belum mengenal secara jelas bagian-bagian mangrove yang dapat digunakan untuk produk pangan dan kesehatan, sehingga produk yang bisa dihasilkan sedikit, masa simpan simplisia yang terlalu singkat dan rentan berjamur yang membuat daya jual produk menjadi rendah, dan pengunjung/pembeli tidak bisa menikmati minuman daun jeruju secara langsung karena tidak adanya bentuk yang bisa diminum secara langsung. Metode Pelaksanaan dalam menyelesaikan permasalahan mitra adalah dengan penyuluhan dan pendampingan terkait permasalahan yang dihadapi oleh mitra. Kegiatan ini memberikan hasil yang sangat memuaskan. Setelah diberikan penyuluhan, mitra akhirnya memahami tentang pemanfaatan hasil turunan tumbuhan mangrove sebagai bahan baku obat tradisional dan makanan serta memahami bagaimana cara mengolah daun jeruju, mulai dari panen, penyortiran, pengeringan dengan oven, pembuatan serbuk simplisia menggunakan grinder, hingga pengemasan dan penyimpanan. Selain itu, mitra juga akhirnya mengetahui cara lain untuk membuat minuman herbal dengan teknik infusa. Teknik infusa terbukti mampu mengekstrak lebih banyak senyawa fitokimia dari daun jeruju yang dibuktikan dengan warna minuman yang keruh kecoklatan serta rasa dan aroma yang khas daun jeruju

EVALUATION OF PHOSPHOMOLYBDENUM AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS IN EXTRACT COMBINATIONS OF MANGIFERA INDICA AND EUPHORBIA HIRTA Putra, I Made Wisnu Adhi; Suarjana, Nyoman
Walisongo Journal of Chemistry Vol. 8 No. 1 (2025): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v8i1.25580

Combining plant extracts at lower doses may enhance antioxidant effects through synergy. This study investigated the antioxidant capacity of combined Mangifera indica and Euphorbia hirta leaf extracts using metal ion-reducing power assays. The extracts were prepared via maceration with 70% ethanol. Phytochemical profiling was conducted through preliminary screening tests. Antioxidant capacity was assessed spectrophotometrically using the phosphomolybdenum and ferric reducing antioxidant power (FRAP) assays. The phosphomolybdenum assay involved incubation of the extracts with reagent at 95°C for 90 minutes, while the FRAP assay was conducted at 37°C for 15 minutes. Extract combinations were prepared in the ratios of 1:3, 1:1, and 3:1. Phytochemical screening revealed the presence of alkaloids, phenolics, flavonoids, steroids, and terpenoids in both extracts. In the phosphomolybdenum assay, the Mo(VI) reducing power of M. indica extract (29.154±0.664 mg AAE/g extract) was higher than that of E. hirta (27.948±0.667 mg AAE/g extract). Similarly, in the FRAP assay, the ferric reducing power of M. indica extract (55.304±1.284 mg AAE/g extract) exceeded that of E. hirta (48.009±1.873 mg AAE/g extract). The highest reducing powers were observed in Comb. 3, yielding 30.745±0.715 mg AAE/g extract (phosphomolybdenum) and 57.190±1.431 mg AAE/g extract (FRAP). Among the three combinations tested, only the 3:1 ratio demonstrated a synergistic antioxidant effect in both assays.

A Synergistic Effect of Free Radical Scavenging Activity of the Combination of Mangifera indica and Euphorbia hirta Extracts Putra, I Made Wisnu Adhi; Suarjana, Nyoman; Mahfur, Mahfur; Hasnawati, Hasnawati
Indonesian Journal of Chemistry Vol 25, No 5 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.104706

Due to its synergistic effect, the extract-extract combination is essential in traditional medicine formulation. The combination of Mangifera indica and Euphorbia hirta extracts can produce a synergistic effect if combined in a proper ratio. This investigation reported the synergistic antioxidant effect of M. indica and E. hirta. The ethanolic extracts of M. indica leaf (MILE) and E. hirta aerial part (EHAE) were acquired by the maceration method, followed by evaporation to remove the solvent. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) tests were used to assess the extracts' capacity to scavenge radicals. MILE and EHAE were combined with a ratio of 1:3 (comb. 1), 1:1 (comb. 2), and 3:1 (comb. 3). This work disclosed that the extract combinations showed all types of interactions. However, of all combinations, only comb. 3 depicted the synergistic interaction caused by the regeneration effect of EHAE on MILE. This finding has important implications for health, particularly disease prevention. M. indica and E. hirta extracts with high phenolic and flavonoid contents can work together to reduce oxidative stress and protect the body from a variety of diseases.

ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential. The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.

ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential. The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.

Strategi Pemasaran Untuk Peningkatan Penjualan Produk Kosmetik Herbal di desa Bitera Gianyar Bali Deswiniyanti, Ni Wayan; Endah, Naftalia Sri; Putra, I Made Wisnu Adhi
Paradharma: Jurnal Aplikasi IPTEK Vol. 9 No. 1 (2025): Paradharma: Jurnal Aplikasi IPTEK
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Dhyana Pura â€“ Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/jpd.v9i1.4227

Usaha mikro Alam Jaya Herbal berada di daerah Desa Bitera Kabupaten Gianyar Provinsi Bali dengan jarak lebih kurang 33 km dari Kampus Universitas Dhyana Pura. Pemilik usaha ini adalah Ibu Novi Yanti yang memiliki keinginan untuk memanfaatkan tanaman sekitar agar dapat dimanfaatkan untuk produk kesehatan dan kosmetik yang organik, alami dan aman untuk digunakan sehari-hari. Alam Jaya Herbal sudah memproduksi beberapa produk diantaranya 1)Minyak kemiri Kirana murni 100%; 2) Minyak Kalpataru herbal; 3) Linglang herbal oil untuk bayi dan anak-anak; 4) Komorebi shampoo; 5) Komorebi kondisioner; 6) Kiredaun face and body soap; 7) Kirara Herbal Anti Lice shampoo (anti kutu); 8) Kenempilo hand & body lotion. Ada beberapa permasalahan yang dihadapi usaha ini antara lain kemasan produk yang kurang menarik sehingga penjualan masih kurang, sulitnya mendapatkan supplier botol kemasan, strategi pemasaran penjualan produk yang masih terbatas. Telah dilakukan pendampingan terkait solusi yang ditawarkan yaitu semua produk sudah memiliki desain kemasan produk yang baru dan menarik, telah disediakan supplier botol kemasan, serta sudah memiliki katalog cetak maupun digital sebagai alat pemasaran. Penjualan produk pada bulan Juli 2019 belum sepenuhnya menggunakan desain yang baru, sedangkan bulan Agustus dan September 2019 sudah 100% menggunakan desain kemasan yang baru. Sehingga dari data dapat dilihat peningkatan penjualan produk masing-masing produk mencapai 20-50%. Luaran pada kegiatan ini adalah seminar nasional, submit artikel pada jurnal ber ISSN serta publikasi pada media online youtube. Disarankan untuk dapat melanjutkan kegiatan pengabdian fokus pada pengurusan ijin masing-masing produk, karena baru hanya satu produk yang sudah memiliki ijin UMOT yaitu minyak kemiri. Perlu diadakan pelatihan untuk destilasi minyak dengan destilator serta pengembangan penjualan produk melaui website.

Pelatihan Pembuatan Herbal Welcome Drink Dan English For Hospitality Bagi Anggota Pokdarwis Desa Selanbawak, Marga, Kabupaten Tabanan I Made Wisnu Adhi Putra; Ni Putu Widya Astuti; Mirsa Umiyati; Yohanes O. L. Awololon; Wirasa, Komang Satria
Joong-Ki : Jurnal Pengabdian Masyarakat Vol. 5 No. 2: Februari 2026
Publisher : CV. Ulil Albab Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56799/joongki.v5i2.15184

Program pengabdian kepada masyarakat ini dilaksanakan untuk meningkatkan kapasitas Kelompok Sadar Wisata (Pokdarwis) Desa Selanbawak melalui dua bidang intervensi utama, yaitu pengolahan herbal welcome drink dan peningkatan kemampuan English for Hospitality. Pelatihan herbal berfokus pada pemanfaatan simplisia daun sambung nyawa serta bahan rempah lokal menggunakan teknik infusa, mulai dari proses persiapan bahan, proporsi takaran, perebusan, penyaringan, hingga penyajian higienis. Hasil evaluasi menunjukkan bahwa peserta mampu memahami standar produksi dan menghasilkan formulasi minuman yang konsisten. Pelatihan English for Hospitality dilakukan melalui pretest, praktik dialog, simulasi pelayanan, dan posttest untuk menilai perubahan kemampuan komunikasi. Hasil pengukuran menunjukkan peningkatan skor rata-rata peserta dari 19,17 pada pretest menjadi 20,83 pada posttest dengan total 20 responden, menandakan peningkatan keterampilan berbahasa dalam konteks pelayanan wisata. Secara keseluruhan, kegiatan ini berhasil meningkatkan keterampilan teknis dan komunikasi anggota Pokdarwis, sekaligus memperkuat kesiapan Desa Selanbawak dalam memberikan layanan wisata yang lebih profesional dan berdaya saing.

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