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A Study of Genetic Variability 30 Rice Genotypes (Oryza sativa L.) Using Some SSR (Simple Sequence Repeat) Markers Adrift Zn: Studi Variabilitas Genetik 30 Genotipe Padi (Oryza Sativa L.) Menggunakan Beberapa Marka SSR (Simple Sequence Repeat) Terpaut Zn Pangaribuan, Tiwi Rumondang; Syafiâ€™i, Muhammad; Azizah, Elia; Susanto, Untung; Pramudyawardani, Furry; Prastika, Desi
Jurnal Online Pertanian Tropik Vol. 9 No. 2 (2022): JURNAL ONLINE PERTANIAN TROPIK
Publisher : Talenta Publisher

Rice is one of the important commodities in indonesia. Analysis of rice genetic diversity is necesarry for the succes of the local rice variety program. This study aims to identify the genetic variability and kinship patterns of 30 rice genotypes (Oryza sativa L.) using six zinc-linked SSR (Simple Sequence Repeat) markers. The research was conducted in September - December 2021 at the DNA Laboratory of the Sukamandi Rice Plant Research Center. A total of 30 local rice genotypes with diverse Zinc content have been analyzed using laboratory experiments. The results showed that there were different allele variations (2 â€“ 8) among the genotypes tested with an average number of alleles of 4.5, while the average value of Polymorphism Information Content (PIC) amounted to 0.48 (0.20 - 0.70). 4 SSR markers have PIC values of > 0.5 (RM162, RM38, RM30, and RM80) which show that the markers are informative for the study of rice genetic diversity with Zinc content variety with an average gene diversity value of 0.53. The results of the phylogenetic analysis showed that the 30 genotypes clustered into five clusters with a similar coefficient of 0.68.

RESPON PERTUMBUHAN BEBERAPA VARIETAS BAWANG MERAH (Allium ascalonicum L.) AKIBAT PEMBERIAN BERBAGAI KONSENTRASI Gibberelic Acid (GA3) DI DATARAN RENDAH KABUPATEN KARAWANG Nursyabani, Alfia; Azizah, Elia; Supriadi, Devie Rienzani
Jurnal Ilmiah Hijau Cendekia Vol 9 No 1 (2024): Jurnal Ilmiah Hijau Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/hijau.v9i1.4217

Peningkatan produksi bawang merah di dataran rendah dapat diupayakan dengan penggunaan varietas unggul dan pemberian Zat Pengatur Tumbuh. Penelitian ini bertujuan untuk mendapatkan konsentrasi GA3 yang berpengaruh terhadap pertumbuhan pada setiap varietas tanaman bawang merah (Allium ascalonicum L.). Penelitian dilaksanakan di screen house Fakultas Pertanian Universitas Singaperbangsa Karawang yang terletak di Desa Pasirjengkol, Kecamatan Majalaya, Kabupaten Karawang, Provinsi Jawa Barat. Penelitian menggunakan Rancangan Acak Kelompok (RAK) Faktorial yang terdiri dari 2 faktor. Faktor pertama varietas bawang merah (S) sebanyak 3 taraf (batu, bauji, maja) dan faktor kedua yaitu konsentrasi GA3 (G) sebanyak 4 taraf (0, 100, 150, dan 200 ppm) dengan setiap perlakuan diulang sebanyak 3 kali, sehingga terdapat 36 satuan percobaan. Hasil data dianalisis dengan uji F taraf 5% dan diuji lanjut menggunakan Duncan Multiple Range Test (DMRT) pada taraf 5% jika terdapat pengaruh yang signifikan. Hasil percobaan menunjukkan faktor mandiri varietas maja memberikan hasil tertinggi pada parameter tinggi tanaman, diameter umbi, tinggi umbi, dan jumlah daun. Faktor mandiri konsentrasi GA3 memberikan pengaruh nyata pada parameter tinggi tanaman di 4 mst dengan konsentrasi 100 ppm.

Karakterisasi Penampilan Agronomi Beberapa Aksesi Bawang Merah (Allium cepa L) di Dataran Rendah Karawang Falah, Ridhwan; Azizah, Elia; Syafi’i, Muhammad
Indonesian Journal of Agrotech Vol. 8 No. 1 (2023): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture Universitas Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar

The demand for onions in Indonesia is always increasing, but shallot production has not been able to meet the needs of the community. The purpose of this study was to obtain the best accessions that can be used in the selection of plant breeding that has the best potential in the Karawang area. So that it can increase the productivity and resistance of shallot plants in the lowlands of Karawang. The onion research was carried out for 3 months, starting from December 2021 to March 2022. Located in UNSIKA New Land, Pasir Jengkol Village, Majalaya District, Karawang Regency, West Java Province. The location of the coordinates is 6ᵒ18'5"LS and 107ᵒ20'24"E, with an altitude of 15 meters above sea level (Google Earth). This study was arranged using a Randomized Block Design (RAK) with 8 treatments (shallot accessions) and 4 replications, in each replication there were 4 samples with a total of 128 experimental units. Data were analyzed using analysis of variance and further test with DMRT (Duncan Multiple Range Test) at 5% level. The results of this experiment showed that there was a significant effect on the appearance of the agronomic characterization of shallot accession on the growth and yield components (number of tillers, wet bulb weight per plant, dry bulb weight per plant). Accession BLR7 (Blue Lancor) gave the highest average yield on the number of tillers of 12.4. Accession BLR7 (Blue Lancor) also gave the highest yield of wet tuber weight per plant, which was 23.84 grams. Accession LKG1 (Lokal Karawang) gave the highest yield on tuber dry weight per plant of 18.58 grams

Analisis Keragaan Karakter Morfologi Delapan Aksesi Bawang Merah (Allium ascalonicum L.) Pada Lahan Sawah di Kabupaten Karawang Maesaroh, Dita; Azizah, Elia; Agustini, Rika Yayu
Indonesian Journal of Agrotech Vol. 8 No. 02 (2023): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture Universitas Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v8i02.7046

Shallots (Allium ascalonicum L.) is a crucial vegetable commodity because it has a high nutritional content and the demand for shallots in Indonesia is increasing. Efforts to meet the demand for shallots in Indonesia are the use of high yielding varieties in order to spur increased productivity of shallots in the lowlands. This study aims to obtain the best results from the analysis of the morphological character of eight red onion accessions in rice fields in Karawang district. The research was carried out in the experimental field of the Faculty of Agriculture, Singaperbangsa Karawang University, which is located in Pasirjengkol Village, Majalaya District, Karawang Regency. The research method used a single factor Randomized Block Design (RAK). There were 8 treatments and repeated 4 times, so there were 32 experimental plots. Data were analyzed using analysis of variance and further test with DMRT (Duncan Multiple Range Test) at 5% level. The results of this experiment showed that there was a significant effect of the appearance of the morphological characters of shallot accessions on the growth components (plant height, number of leaves, and number of bulbs). The BM5 (Bima) treatment gave the best results on plant height of 33.25/cm, and BR6 (Berlind) treatment gave the best results on the number of leaves of 42.40/strand, and the number of tubers of 16.50/ clove.

Variabilitas Genetik pada Beberapa Varietas Unggul Baru Padi (Oryza sativa L.) Berdasarkan Penanda Morfologi Biji Ardani, Hanna Qotrunnada; Syafii, Muhammad; Azizah, Elia; Susanto, Untung
JURNAL AGROPLASMA Vol 11, No 2 (2024): JURNAL AGROPLASMA VOLUME 11 NO 2 TAHUN 2024
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v11i2.5906

Rice (Oryza sativa L.) is known to have a very diverse genetic diversity. The target of rice variety improvement is to produce new varieties that have superior traits in accordance with the development objectives in each typological region. This study aims to determine the level of similarity formed in a number of new superior varieties. The method used is descriptive experimental, with 51 treatments, namely new superior varieties, repeated 3 times so that there are 132 experimental units. Each agronomic character observation data was analyzed by clustering using the UPGMA method. The analysis used NTSYS 2.02i and 2.11a software. The results showed that the genetic variability of 51 new superior varieties of rice (Oryza sativa L.) has a similarity coefficient value of 0.81 based on clustering analysis. This shows a high level of similarity of genetic variability, however, the clustering results form 5 main groups with genetic distances ranging from 0.0000 - 0.5593. Keywords: genetic variability, seed morphology, new improved varieties, clustering.

Pengaruh Pelapisan Pektin Kulit Buah Lemon Lokal Terhadap Viabilitas Benih Timun Apel (Cucumis Sp.) pada Periode Simpan Berbeda Rizki, Muhammad; Azizah, Elia; Samaullah, H.M. Yamin; Syafi’i, Muhammad; Saputro, Nurcahyo Widyodaru
JURNAL AGROPLASMA Vol 11, No 2 (2024): JURNAL AGROPLASMA VOLUME 11 NO 2 TAHUN 2024
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v11i2.5934

High-quality seeds are available for planting, and proper storage is necessary. High-quality seeds can experience deterioration during storage, and one alternative to address this issue is by applying seed coating treatments. The research aims to investigate the effect of local lemon peel pectin coating on the viability of apple cucumber seeds (Cucumis sp) and determine the optimal concentration of lemon peel pectin for seed coating. The experiment was conducted at the Agronomy Laboratory, Faculty of Agriculture, Universitas Singaperbangsa Karawang, from November 2023 to March 2024. The method used was an experimental approach with a Completely Randomized Design (CRD) consisting of 15 treatments with 3 replications: A (0 g + 4 weeks), B (0 g + 8 weeks), C (0 g + 12 weeks), D (0.5 g + 4 weeks), E (0.5 g + 8 weeks), F (0.5 g + 12 weeks), G (1 g + 4 weeks), H (1 g + 8 weeks), I (1 g + 12 weeks), J (1.5 g + 4 weeks), K (1.5 g + 8 weeks), L (1.5 g + 12 weeks), M (2 g + 4 weeks), N (2 g + 8 weeks), O (2 g + 12 weeks). The treatment effects were analyzed using analysis of variance (ANOVA), and if the F-test at the 5% significance level was significant, further Duncan Multiple Range Test (DMRT) was conducted to determine the best treatment. The results showed significant effects on germination capacity, vigor index, and growth rate. The best treatment was found in treatment C (0 g + 12 weeks), which achieved the highest germination capacity of 90.30%, and treatment B (0 g + 8 weeks) had the highest vigor index for seed storage at 76.97%. Keywords: apple cucumber, lemon zest, pectin, seed coating

Pengaruh Pelapisan Menggunakan Antioksidan Alami Dari Kulit Buah Naga Pada Periode Simpan Berbeda Terhadap Viabilitas Benih Timun Apel (Cucumis sp.) Fatha, Muhammad Rafi Aryu; Azizah, Elia; Samaullah, H.M. Yamin; Syafi’i, Muhammad; Saputro, Nurcahyo Widyodaru
JURNAL AGROPLASMA Vol 11, No 2 (2024): JURNAL AGROPLASMA VOLUME 11 NO 2 TAHUN 2024
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v11i2.5946

The deterioration of seed quality cannot be repaired or prevented, but it can be minimised by proper processing and storage. An alternative to overcome this problem is to provide seed coating treatment. The study aimed to determine the effect of antioxidant coating of red dragon fruit peel on the viability of apple cucumber seeds (Cucumis. Sp) and to obtain the best dose of dragon fruit peel antioxidant to coat apple cucumber seeds (Cucumis sp). The experiment was conducted at Lab.Agronomy, Faculty of Agriculture, Singaperbangsa University of Karawang, starting from Nov. 2023 - Mar. 2024. The experimental method used was experimental method and the experimental design used was a completely randomised design (CRD) consisting of 15 treatments in 3 replications, so there were 45 experimental units. The effect of treatment was analysed with variance analysis and if the F test at the 5% level was significant, then to find out the best treatment continued with the DMRT (Duncan Multiple Range Test) further test at the real level of 5%. The results showed a significant effect of treatment on germination, vigour index, and growth speed. The best treatment was obtained in treatment B (0 g + 8 weeks) which was able to produce the highest value in the parameters of germination power of 75.76%, vigour index of 9.09%, and growth speed of 10.30%/day. Keywords: apple cucumber, seed coating, antioxidant, dragon fruit peel, seed storage

Keragaan karakter morfo-agronomi beberapa aksesi bawang merah (Allium cepa L.) lokal jawa berdasarkan analisis multivariat Azizah, Elia; Ardiyansah, Ardiyansah; Fauzi, Iqbal
Jurnal AGRO Vol 11, No 2 (2024)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/38577

Shallot production still fluctuative in several production centers such as Brebes due to the lack of new superior varieties with a high level of adaptation in areas of Indonesia that are prone to damage from land conversion, weather, and low technology application. The study aimed to obtain the best morphological and agronomic appearance of lowland shallots accessions through clustering analysis, and to obtain the limiting characters that provide the highest variation in the population. The research was conducted at the Experimental Farm, Faculty of Agriculture, Singaperbangsa University of Karawang in Pasirjengkol Village, West Java. Field trials were conducted during one growing season using 8 accessions of shallots from different regions, including the accession of Cikijing, Pati, Nganjuk, Trisula, Bima, Berlin, Maja, and Bandung with 15 observed morpho-agro characters. The research was conducted using a single-factor randomized block design with 4 replications, further tested using cluster and principal component analysis (PCA). The results showed that the level of similarity of Trisula accession was very different from other accessions (0.2) for the widest diameter and tuber shape characters. In contrast, the accessions Berlin and Maja have the same morpho-agro appearance (0.8) in tuber diameter, root tip shape, tuber shape, tuber skin thickness, leaf color, crown curvature, and tuber color. The limiting characteristics causing the highest variation in the population are the dry weight of tubers per plant and the shape of the tip tuber stem. ABSTRAK Produksi bawang merah masih fluktuatif di beberapa sentra bawang merah seperti brebes, hal ini akibat belum adanya varietas unggul baru yang memiliki tingkat adaptasi luas pada wilayah di Indonesia yang cenderung mengalami kerusakan akibat alih fungsi lahan, cuaca, dan rendahnya penerapan teknologi. Tujuan penelitian untuk mendapatkan aksesi bawang merah yang memiliki penampilan morfologi dan agronomi terbaik di dataran rendah melalui analisis klaster serta mendapatkan karakter pembatas yang memberikan variasi tertinggi pada populasi. Penelitian dilaksanakan dikebun percobaan Fakultas Pertanian Universitas Singaperbangsa Karawang di Desa Pasirjengkol, Jawa Barat. Percobaan lapangan dilaksanakan selama satu musim tanam dengan 8 aksesi Bawang Merah (Allium ascalonicum L.) yang diambil dari berbagai wilayah diantaranya yaitu aksesi Cikijing, Pati, Nganjuk, Trisula, Bima, Berlin, Maja, dan Bandung berdasarkan 15 karakter morfo-agro yang diamati. Penelitian dilakukan dengan rancangan acak kelompok faktor tunggal dengan 4 ulangan, kemudian diuji lanjut dengan analisis kluster dan komponen utama (principle component analysis). Hasil penelitian menunjukkan bahwa tingkat kemiripan (similarity) aksesi Trisula jauh berbeda dengan aksesi lainnya (0,2) untuk karakter diameter terluas dan bentuk umbi. Berbeda dengan aksesi Berlin dan Maja yang memiliki penampilan morfo-agro yang sama (0,8) pada diameter umbi, bentuk ujung akar, bentuk umbi, ketebalan kulit umbi, warna daun, kelengkungan tajuk, dan warna umbi. Adapun karakter pembatas yang menyebabkan variasi tertinggi pada populasi adalah bobot kering umbi per tanaman dan bentuk ujung batang umbi.

Keragaan karakter morfo-agronomi beberapa aksesi bawang merah (Allium cepa L.) lokal jawa berdasarkan analisis multivariat Azizah, Elia; Ardiyansah, Ardiyansah; Fauzi, Iqbal
Jurnal AGRO Vol. 11 No. 2 (2024)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/38577

Shallot production still fluctuative in several production centers such as Brebes due to the lack of new superior varieties with a high level of adaptation in areas of Indonesia that are prone to damage from land conversion, weather, and low technology application. The study aimed to obtain the best morphological and agronomic appearance of lowland shallots accessions through clustering analysis, and to obtain the limiting characters that provide the highest variation in the population. The research was conducted at the Experimental Farm, Faculty of Agriculture, Singaperbangsa University of Karawang in Pasirjengkol Village, West Java. Field trials were conducted during one growing season using 8 accessions of shallots from different regions, including the accession of Cikijing, Pati, Nganjuk, Trisula, Bima, Berlin, Maja, and Bandung with 15 observed morpho-agro characters. The research was conducted using a single-factor randomized block design with 4 replications, further tested using cluster and principal component analysis (PCA). The results showed that the level of similarity of Trisula accession was very different from other accessions (0.2) for the widest diameter and tuber shape characters. In contrast, the accessions Berlin and Maja have the same morpho-agro appearance (0.8) in tuber diameter, root tip shape, tuber shape, tuber skin thickness, leaf color, crown curvature, and tuber color. The limiting characteristics causing the highest variation in the population are the dry weight of tubers per plant and the shape of the tip tuber stem. ABSTRAK Produksi bawang merah masih fluktuatif di beberapa sentra bawang merah seperti brebes, hal ini akibat belum adanya varietas unggul baru yang memiliki tingkat adaptasi luas pada wilayah di Indonesia yang cenderung mengalami kerusakan akibat alih fungsi lahan, cuaca, dan rendahnya penerapan teknologi. Tujuan penelitian untuk mendapatkan aksesi bawang merah yang memiliki penampilan morfologi dan agronomi terbaik di dataran rendah melalui analisis klaster serta mendapatkan karakter pembatas yang memberikan variasi tertinggi pada populasi. Penelitian dilaksanakan dikebun percobaan Fakultas Pertanian Universitas Singaperbangsa Karawang di Desa Pasirjengkol, Jawa Barat. Percobaan lapangan dilaksanakan selama satu musim tanam dengan 8 aksesi Bawang Merah (Allium ascalonicum L.) yang diambil dari berbagai wilayah diantaranya yaitu aksesi Cikijing, Pati, Nganjuk, Trisula, Bima, Berlin, Maja, dan Bandung berdasarkan 15 karakter morfo-agro yang diamati. Penelitian dilakukan dengan rancangan acak kelompok faktor tunggal dengan 4 ulangan, kemudian diuji lanjut dengan analisis kluster dan komponen utama (principle component analysis). Hasil penelitian menunjukkan bahwa tingkat kemiripan (similarity) aksesi Trisula jauh berbeda dengan aksesi lainnya (0,2) untuk karakter diameter terluas dan bentuk umbi. Berbeda dengan aksesi Berlin dan Maja yang memiliki penampilan morfo-agro yang sama (0,8) pada diameter umbi, bentuk ujung akar, bentuk umbi, ketebalan kulit umbi, warna daun, kelengkungan tajuk, dan warna umbi. Adapun karakter pembatas yang menyebabkan variasi tertinggi pada populasi adalah bobot kering umbi per tanaman dan bentuk ujung batang umbi.

Evaluasi Sistem Tanam Tumpangsari Beberapa Galur Hibrida Jagung (Zea mays L.) dengan Bawang Merah (Allium ascalonicum L.) Supriadi, Devie Rienzani; Rianti, Winda; Muharam, Muharam; Azizah, Elia; Bastamansyah, Bastamansyah; Sugiarto, Sugiarto; Sugiono, Darso; Rahmi, Hayatul; Ruswandi, Dedi
Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Vol. 22 No. 2 (2024): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v22i2.2676

Sistem tanam tumpangsari telah lama diterapkan dalam praktik pertanian sebagai upaya untuk meningkatkan efisiensi lahan dan hasil panen, terutama pada tanaman pangan. Salah satu kombinasi yang potensial adalah tumpangsari tanaman jagung dan bawang merah. Penelitian ini bertujuan untuk mendapatkan kombinasi galur hibrida jagung (Zea mays L.) dengan tanaman bawang merah (Allium ascalonicum L.) terbaik berdasarkan evaluasi sistem tanam tumpangsari. Penelitian dilakukan di lahan Politeknik Kelautan dan Perikanan, Karangpawitan, Kec. Karawang Barat, Kab. Karawang pada Februari-Mei 2024. Metode yang digunakan adalah Rancangan Acak Kelompok (RAK) faktor tunggal yang terdiri dari 13 perlakuan dengan 3 kali ulangan: V1; V2; V3; V4; V5; V6; V7; V8; V9; V10; V11; V12; V13. Pengaruh perlakuan dianalisis dengan analisis ragam (ANOVA) uji F taraf 5%. Apabila uji F signifikan, dilanjutkan dengan uji lanjut DMRT (Duncan Multiple Range Test) pada taraf 5%. Hasil evaluasi tumpangsari menunjukkan perlakuan V10 memiliki nilai tertinggi pada LER sebesar 3,37 dan Agresivitas sebesar 0,27. Perlakuan V12 memiliki nilai RCC tertinggi 7,43 dan perlakuan V1 memiliki nilai CR tertinggi 10,68 pada tanaman jagung.

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