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Evaluation of the Grade of Hatching Eggs of Sensi-1 Agrinak and KUB Crossbred Chicken on Hatching and Production Performances Aulia Fitriawati Polontalo; Rudi Afnan; Sri Darwati; Andi Yulyani Fadwiwati
ANIMAL PRODUCTION Vol. 25 No. 1 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.1.186

Abstract

The potential of Sensi-1 Agrinak and KUB crossbred so-called SENKUB chicken as dual-purpose chicken can be increased by selecting hatching egg weights to get DOC with good quality. The study was conducted to evaluate the grades of hatching eggs of SENKUB chicken on hatching and production performances. The complete randomized design was applied in this experiment with 3 treatments. The treatments are the grades of hatching egg (small 36-40 g, medium 41-45 g, and large 46-50 g). The hatching process used two stages hatching machine. All DOCs were selected according to the Indonesia National Standard (SNI). The selected DOCs were raised in different pens to evaluate the production performances. The hatching showed that the large eggs have significantly the highest fertility, hatchability, hatching weight, and salable chick but the lowest weight loss. Embryo mortality was the same between different hatching egg grades. The best-weight DOC was obtained from medium and large hatching eggs. The hatching egg grades do not significantly affect the production performances of the hatched chicken. However, the chickens with small and large hatching eggs resulted in better production performance.
Reduce Heat Stress on Broilers During Transport by Supplying Drinking Water Insan Mujahid Afnan; Niken Ulupi; Rudi Afnan
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.26391

Abstract

ABSTRACT. The transportation process causes heat stress in broilers. This research aims to test the effectiveness of the drinking water supply system for broilers during transport to reduce the effects of heat stress caused by the transportation process. Broilers are grouped into four treatments: morning transport with water (T1), morning transport without water (T2), afternoon transport with water (T3), and afternoon transport without water (T4). Results show that broilers consume more water (317.26 ml/bird/hour) during morning than during afternoon transport (61.53 ml/ bird/hour). However, these numbers are estimated to be lower, with water spillage contributing to the high consumption, especially during morning transport. Rectal temperature for birds is lower in T1 and T3 broilers compared to T2 and T4 broilers, although still within normal range. A decrease in hematocrit and blood glucose levels while still at normal levels is observed for all treatment groups. An increase in the ratio of heterophile and lymphocyte (HL ratio) is observed within the normal range except for T1. Percentage of body weight loss is lower on broilers with access to water: T1 (3.5%) and P3 (4.4%) compared to broilers without access to water: T2 (4.0%) and T4 (5.0%) in the same time of transport although not statistically significant (P0.05). Based on weight loss percentage, it can be concluded that water intake during transport has some effectiveness in reducing heat stress with the best results shown on broilers transported in the morning with drinking water..(Mengurangi cekaman panas pada ayam broiler saat transportasi melalui pemberian air minum)ABSTRAK. Proses transportasi menyebabkan ayam mengalami cekaman panas. Penelitian ini bertujuan untuk mengkaji efektifitas pemberian air minum selama transportasi dalam upaya mengurangi dampak cekaman panas dan meningkatkan kesejahteraan hewan dengan cara mengurangi dehidrasi dan cekaman yang disebabkan proses transportasi pada ayam broiler. Ayam dikelompokkan menjadi empat perlakuan: transportasi pagi dengan air minum (T1), transportasi pagi tanpa air minum (T2), transportasi siang dengan air minum (T3), dan transportasi pagi tanpa air minum (T4). Hasil menunjukkan ayam lebih banyak mengonsumsi air (317,26 ml/ekor/jam) pada transportasi pagi daripada transportasi siang (61,53 ml/ekor/jam). Namun, nilai ini diestimasi lebih rendah dari yang didapatkan, dengan air yang tumpah berkontribusi terhadap tingginya konsumsi air, terutama pada transportasi pagi hari. Suhu rektal lebih rendah pada broiler T1 dan T3 dibandingkan T2 and T4 namun berada dalam rentang normal. Penurunan kadar glukosa darah dan hematokrit terjadi pada seluruh perlakuan. Peningkatan rasio HL terjadi pada seluruh perlakuan kecuali T1. Persentase penurunan bobot badan lebih rendah pada ayam yang diberi air minum T1 (3,5%) dan T3 (4,4%) dibandingkan dengan ayam yang tidak diberi air minum T2 (4,0%) dan T4 (5,0%) pada waktu transportasi yang sama meskipun secara statistik tidak signifikan (P0,05). Berdasarkan perbedaan persentase penurunan bobot badan, dapat disimpulkan bahwa konsumsi air mengurangi dampak cekaman panas yang dialami ayam broiler selama transportasi dengan hasil terbaik didapatkan pada transportasi pagi dengan air minum.
Pengaruh Polimorfisme Gen Heat Shock Protein 70 (HSP70) SacII terhadap Toleransi Panas Itik Lokal Sumatera Barat Kusnadidi Subekti; Dedi Duryadi Solihin; Rudi Afnan; Asep Gunawan; Cece Sumanri
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14876

Abstract

ABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702TC, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi.(The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck)ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702TC, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status.
Carcass and Chemical Quality of IPB D1 Chicken Meat in Free-range and Intensive Systems Kartika, Dewi; Ulupi, Niken; Afnan, Rudi; Wulandari, Zakiah
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.204

Abstract

IPB D1 chicken is a local chicken resulting from crossing pelung, sentul, kampung, and parent stock broiler strain Cobb chickens. IPB D1 chicken was released through a decree by the Ministry of Agriculture with No. 693/KPTS/PK. 230.M/9/2019. Free-range is a system of raising farm animals, including chickens, by providing access to outdoor running (pasture) to exercise, find more feed, and be exposed to more oxygen. This study analyzed the carcass percentage, edible carcass, non-edible carcass, and chemical quality of IPB D1 chickens raised in a free-range system. A total of 50 IPB D1 male chickens were raised in free-range and intensive systems for four weeks. The microclimatic data were analyzed descriptively. Meanwhile, carcass percentage and physicochemical and organoleptic properties of IPB D1 chicken meat were subjected to a t-test. Free-range and intensive systems showed no significant effects on the carcass, commercial cuts, edible and non-edible percentages, and MDA quality traits of IPB D1 male chicken thigh meat. The cholesterol content of IPB D1 thigh meat in a free-range system was significantly lower than in an intensive system Keywords: carcass, free-range, IPB D1 chicken, cholesterol, MDA
Performance and Physicochemical Quality of Quail Eggs Given Bandotan (Ageratum conyzoides L.) Leaf Extract Hariono, Hariono; Kaiin, Ekayanti Mulyawati; Suryati, Tuti; Ulupi, Niken; Afnan, Rudi
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.780

Abstract

The purpose of this study was to determine the influence of bandotan (Ageratum conyzoides L.) leaf extract on the production performance and physicochemical quality of quail eggs raised in tropical settings with ambient temperatures that exceed the comfort zone. A total of 200 female quails aged 6 weeks were reared in colony cages for 30 days. They were separated into four treatments with five replications, each with ten quails. Bandotan leaf extract was administered in dosages of 0%, 0.15%, 0.30%, and 0.45% per quail daily. The parameters measured included feed intake, feed conversion, egg production, egg mass, mortality, egg physical quality, and cholesterol and malondialdehyde (MDA) levels in egg yolks. Adding bandotan leaf extract to drinking water significantly improved quail production performance (p < 0.05). A dose of 0.30% resulted in the highest feed consumption, output, and egg mass, as well as significantly lower mortality rates, indicating its effectiveness in boosting the productivity and health of laying quails. Although it did not have a significant influence on the physical quality of eggs, administration of the extract at that dose significantly reduced MDA levels, egg cholesterol levels, and boosted antioxidant activity (p < 0.05). This demonstrates that bandotan leaf extract at a dose of 0.30% is beneficial in improving the production performance and chemical quality of heat-stressed quail eggs while preserving their physical quality. Keywords: bandotan, eggs, malondialdehyde, productive performance, quail
Penerapan Kesejahteraan Hewan dalam Manajemen Ayam Broiler Hariono, Hariono; Priyambodo, Danang; Ulupi, Niken; Afnan, Rudi
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 26 No 2 (2024): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.26.2.98-111.2024

Abstract

Ayam broiler tetap menjadi prioritas utama dalam memenuhi kebutuhan protein hewani masyarakat. Mayoritas ayam broiler diproduksi dan dipelihara secara intensif di dalam kandang. Sistem pemeliharaan ini mulai menarik perhatian masyarakat karena dianggap membatasi aktivitas hidup ayam dan mengurangi kesejahteraan mereka. Kesejahteraan hewan harus diperhatikan sejak persiapan kandang, manajemen pemeliharaan hingga panen. Memperhatikan kesejahteraan hewan tidak hanya menguntungkan hewan itu sendiri tetapi juga peternak dan konsumen manusia. Penelitian ini dilakukan berdasarkan studi literatur dengan menganalisis data sekunder dari berbagai jurnal yang telah diterbitkan. Penerapan kesejahteraan hewan dalam manajemen pemeliharaan ayam broiler meliputi persiapan kandang, proses pemeliharaan, hingga ayam siap dipanen. Dengan penerapan kesejahteraan hewan, ayam akan mendapatkan kondisi lingkungan yang nyaman dan sesuai kebutuhan sehingga dapat berproduksi secara optimal dan efisien. Penelitian mengenai standar manajemen pemeliharaan ayam broiler menunjukkan bahwa penerapan kesejahteraan hewan pada hewan ternak secara tidak langsung sudah dilakukan.
Aktivitas Farmakologis Ekstrak Daun Bandotan (Ageratum conyzoides L.) dalam Bidang Kesehatan Hewan: Pharmacological Activity of Bandotan Leaf Extract (Ageratum conyzoides L.) in Animal Health Hariono, Hariono; Kaiin, Ekayanti Mulyawati; Suryati, Tuti; Ulupi, Niken; Afnan, Rudi
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 27 No 3 (2025): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.27.3.179-188.2025

Abstract

Kesehatan hewan memiliki peranan yang sangat penting dalam mendukung produktivitas ternak, kesejahteraan hewan, serta kesehatan masyarakat veteriner. Seiring dengan meningkatnya kekhawatiran terhadap dampak negatif penggunaan obat-obatan kimia, seperti antibiotik dan antioksidan sintetis, khususnya yang berkaitan dengan risiko resistensi antimikroba, toksisitas, serta residu, muncul kebutuhan akan alternatif alami yang lebih aman dan berkelanjutan. Salah satu tanaman yang berpotensi memenuhi kebutuhan tersebut adalah bandotan (Ageratum conyzoides Linn.). Tanaman ini mengandung berbagai senyawa fitokimia seperti flavonoid, fenol, alkaloid, dan tanin yang memiliki aktivitas farmakologis sebagai antioksidan, antimikroba, antiinflamasi, serta membantu menurunkan kadar kolesterol. Penelitian ini merupakan studi literatur dengan analisis deskriptif terhadap data sekunder dari jurnal dan artikel terakreditasi nasional dan internasional. Berdasarkan kajian pustaka dari berbagai sumber ilmiah, Ekstrak daun bandotan efektif menghambat peroksidasi lipid, menurunkan kolesterol dan trigliserida, serta meningkatkan bobot badan pada tikus. Pada ayam broiler, ekstrak ini menurunkan kolesterol, meningkatkan pertumbuhan, efisiensi pakan, kualitas daging, dan berfungsi sebagai agen antikoksidia alami.
PRODUCTION PERFORMANCE OF QUAIL (Coturnix-coturnix japonica) AND IDENTIFICATION OF IgY CONCENTRATION IN BLOOD SERUM AND YOLK Tarigan, Indri Nur Krisna; Afnan, Rudi; Murtini, Sri; Ulupi, Niken
Jurnal Kedokteran Hewan Vol 18, No 3 (2024): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i3.37649

Abstract

This research was conducted to provide information on the production performance of quail parent stock and its offspring with various IgY concentrations. A total of 42 female quail parents stock aged five weeks old were used in this study. Five quails were categorized as medium IgY group with concentrations of 431.12-531.87 g/mL and 37 quails were categorized as low IgY group with concentration of 251.87-384.68 g/mL. The variables observed were the production performance such as initial body weight, feed intake, final body weight, body weight gain, number of eggs, egg mass, egg weight, egg laying intensity, feed conversion, morbidity, and mortality rate and IgY concentration of the yolk of the offsprings. Production performance of female quail with different concentrations of IgY was not significantly different (P0.05). IgY concentration of offsprings in egg yolk was also not significantly different (P0.05) in both groups. The results showed that female quails parent stock with low blood IgY showed better production. Meanwhile, female quail parents stock with moderate blood IgY concentrations produced offspring with high-maternal IgY concentrations in yolk.
PENENTUAN KONSENTRASI SODIUM DODECYL SULFATE DALAM PENGENCER RINGER LAKTAT-KUNING TELUR UNTUK PRESERVASI SEMEN AYAM PELUNG (Determination of Sodium Dodecyl Sulfate Concentration in Ringer Lactate-Egg Yolk Extender for Pelung Rooster Semen Preservation) Hidayat, Numan; Sumantri, Cece; Afnan, Rudi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5091

Abstract

The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.081.44% and 80.821.30%) which were significantly higher (P0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.170.56% and 4.650.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.