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All Journal Bioscience Quagga Journal of Degraded and Mining Lands Management Elkawnie Jurnal Kelautan Tropis Universa Medicina Jurnal Biologi Tropis Jurnal Biotek JURNAL BIOSHELL Bioscientist : Jurnal Ilmiah Biologi Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Kenanga : Journal of Biological sciences and Applied Biology Prosiding Seminar Nasional Universitas Serambi Mekkah Jurnal Pengabdian Masyarakat Indonesia (JPMI) Biocaster : Jurnal Kajian Biologi IJES : Indonesian Journal of Environmental Sustainability. Aksi Nyata : Jurnal Pengabdian Sosial dan Kemanusiaan Bumi: Jurnal Hasil Kegiatan Sosialisasi Pengabdian Kepada Masyarakat Jurnal Pengabdian Masyarakat Indonesia
Arif Sardi, Arif
Universitas Islam Negeri Ar-Raniry
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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ENUMERATION AND PATHOGENICITY TEST OF Vibrio Sp ON Green Mussels (Perna viridis) FROM THE KRUENG CUT AREA OF ACEH BESAR Syafrina Sari Lubis; Arif Sardi; Nailul Muna
KENANGA : Journal of Biological Sciences and Applied Biology Vol 2 No 1 (2022): April 2022
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.976 KB) | DOI: 10.22373/kenanga.v2i1.1920

Abstract

Kerang hijau (Perna viridis) merupakan jenis biota memiliki kandungan zat gizi yang baik untuk dikonsumsi dan nilai ekonomis. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri Vibrio sp., melakukan enumerasi total koloni dengan metode Total Plate Count dan uji patogenitas Vibrio sp. dengan media Blood Agar plate (BAP). Isolasi dengan menggunakan media selektif Thiosulfate Citrate Bile Salts (TCBS) diperoleh 16 isolat. Hasil uji biokimia TSIA (Triple Sugar Iron Agar), uji MRVP (Methyl Red Voges- Proskauer), uji katalase, uji oksidasi, uji SIM (Sulfide Indole Motility) dan Uji Simmon Citrat, menunjukkan isolat Vibrio sp. memiliki kemampuan yang berbeda dalam menghidrolisis media pengujian. Enumerasi total koloni VP10 dengan jumlah 4,63 x 102 Cfu/g sedangkan hasil terendah yaitu isolat VP14 yang diperoleh dengan TPC adalah 2,36 x 102 Cfu/g.. Hasil pengujian patogenitas 16 isolat bersifat patogen karena memiliki kemampuan melisiskan darah yang terdapat didalam media Blood Agar Plate dan bersifat beta hemolisis.
KONSTRUKSI PRIMER UNTUK MENGISOLASI GEN EKSOGLUKANASE BACILLUS SP. RP1 Arif Sardi
Prosiding Seminar Nasional USM Vol 2, No 1 (2019): Semnas Multidisiplin Ilmu
Publisher : Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2169.658 KB)

Abstract

Primer design is a very important factor in gene isolation. The selection of the right primer will determine the success/failure of gene isolation. Information and data on exoglucanase gene sequences were obtained from www.cazy.org and www.ncbi.nlm.nih.gov. The degenerate primer design process is carried out by finding sequences of exoglucanase genes from bacteria that are taxonomically close to Bacillus. By considering some of the criteria needed to design a primer, two primer candidates were produced  at the residual position 1994-2010 and 3112-3129. The first candidate can be constructed as a forward primer and the second as a reverse primer, so the size of the PCR product resulted is ~1100 bp. In addition to the size, the alternative PCR product it is also possible to measure ~800 bp because the results of the alignment of some sequences still indicate a gap between the sequences (about 300 residues). The calculation result of the primer characteristic show that the two primers that were designed were in a fairly ideal condition. Tm  of the two primers is not too much different (forward primer 64.29ºC and reverse primer 63.79ºC) so that it can be used simultaneously in a PCR reaction.
Bioinformatics: Challenges in Integrating Biological Information Arif Sardi
Jurnal Biologi Tropis Vol. 22 No. 4 (2022): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i4.4346

Abstract

Bioinformatics has become a buzzword in today's world of science. About twenty or thirty years ago, people saw biology and computer science as two completely different fields. However, at present, it seems that a new bridge has been built that connects these two fields, and this new field is called bioinformatics. Bioinformatics is an challenging and exciting new multidisciplinary field. This field combines methods of statistics, mathematics, computer science and information technology to solve problems based on biological data. Advances in experimental technology have generated large amounts of data. Therefore, managing and extracting useful information from these sources is essential if we are to discover new knowledge to advance our understanding of life. In this article, we try to discuss what is bioinformatics?, The basic knowledge needed to understand bionformatics and the reasons that are very important for biologists to learn and apply bioinformatics. In addition, we also discuss an introduction to biological terminology that will be widely used in the field of bioinformatics. We are confident that this review will provide valuable insights and serve as a starting point for researchers to become acquainted with and study bioinformatics.  
PENGARUH PERLAKUAN MEDIA AIR TERHADAP PERTUMBUHAN PAKCOY (Brassica rapa) SECARA HIDROPONIK DENGAN TEKNIK DFT (Deep Flow Technique) Lina Rahmawati; Nabilla Munawarah; Arif Sardi; Jamaluddinsyah
KENANGA : Journal of Biological Sciences and Applied Biology Vol 2 No 2 (2022): Oktober 2022
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v2i2.2384

Abstract

Hidroponik adalah salah satu metode budidaya tanaman yang tidak menggunakan media tanah, tetapi memanfaatkan air. Metode hidroponik pada pertumbuhan tanaman pakcoy dapat menggunakan Deep Flow Technique (DFT), yang mensirkulasrikan larutan nutrisi dan media tanam secara terus menerus selama 24 jam. Penelitian ini bertujuan untuk mengetahui respon pertumbuhan tanaman pakcoy (Brassica rapa) dan mengetahui bagaimana perbedaan hasil pertumbuhan tanaman pakcoy (Brassica rapa) dengan perlakuan Air AC dan Air PDAM menggunakan teknik DFT. Penelitian ini dilakukan pada tanggal 16 September – 1 November 2021 di Kebun Biologi Fakultas Sains dan Teknologi Universitas Islam Negri Ar-raniry. Analisis data dilakukan menggunakan Uji Independent Sampel T-Test dengan 2 perlakuan media tanam, perlakuan 1 media menggunakan air AC dan perlakuan 2 menggunakan air PDAM. Berdasarkan hasil Uji Independent sampel T-Tes dari parameter tinggi tanaman, lebar daun, panjang daun, jumlah daun, berat basah, dan panjang akar memiliki hasil tidak dapat perbedaan nyata atau tidak terdapat perbedaan yang signifikan antara pemberian media tanam air AC dan air PDAM dan memiliki nilai P > 0,5. Sedangkan pada parameter tekstur akar memiliki bentuk akar tunggang dengan serabut yang banyak, berwarna coklat dan berukuran panjang.
Pemanfaatan Limbah Organik Rumah Tangga Untuk Pembuatan Pupuk Kompos di Desa Alue Sungai Pinang Kecamatan Jeumpa Kabupaten Aceh Barat Daya Sardi, Arif; Khairatul Ulya
JURNAL PENGABDIAN MASYARAKAT INDONESIA Vol. 2 No. 1 (2023): Februari : Jurnal Pengabdian Masyarakat Indonesia
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jpmi.v2i1.1125

Abstract

This research aims to raise public awareness of the importance of utilizing household waste to make compost in Alue Sungai Pinang Village, Sub-District Jeumpa, Southwest Aceh Regency. By making compost, the household waste can be used as fertilizer and help create a clean environment from waste (zero waste). The method used in this research consists of 3 stages, namely: observation, preparation and implementation of activities. Compost is produced using materials originating from the household waste of Alue Sungai Pinang Village residents in the form of vegetable remains, rotting fruits, sawdust, and manure. Household waste that previously had no economic value can be processed or reused to become compost. This compost fertilizer can be applied to residents' plantations or to grow plants around the house such as vegetables, fruits and decorative plants.
Harnessing hyperaccumulator (Brassica oleracea var. alboglabra) extract for green synthesis of nickel oxide nanoparticles: A prospective route for post-phytoremediation Hamdan, Abd Mujahid; Maulida, Zahratul; Lubis, Syafrina Sari; Sardi, Arif; Reksamunandar, Rhyan Prayuddy; Nisah, Khairun; Malik, Jamaludin
Journal of Degraded and Mining Lands Management Vol. 11 No. 4 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.114.6427

Abstract

Even though phytoremediation is considered a green technology for remediating heavy metals, there are some problems with the application of this technology, particularly when it comes to managing the biomass that is used. So, processing biomass needs to be given a lot of attention. This study outlined the utilization of extracts obtained from the hyperaccumulator plant Brassica oleracea var. alboglabra to synthesize nickel oxide nanoparticles. Subsequently, the nanoparticle underwent testing to determine its suitability as an absorbent for heavy metals, specifically lead, as well as its efficacy as an antifungal agent against Fusarium sp. strain. The characterization of nickel oxide nanoparticles involved several measurements, such as scanning electron microscopy analysis, high- and low-resolution transmission electron microscopy, energy dispersive spectroscopy, X-ray diffraction, and hysteresis curve acquisition. The research findings indicate that the extract from hyperaccumulators can be utilized for the synthesis of NiO, which exhibits an absorption capacity exceeding 98% and serves as an efficient antifungal agent against Fusarium sp. pathogens. The approach utilized in this study not only prioritizes "green" and sustainability factors but also takes into account the economic aspects associated with the items being manufactured. The research has important implications in two areas. Firstly, it demonstrates the utilization of natural resources (B. oleracea var. alboglabra) in the production of nickel oxide, which serves as a safer and more eco-friendly substitute for dangerous chemicals. Furthermore, it aids in the advancement of novel techniques for effectively managing biomass hyperaccumulators.
Pembuatan Pestisida Nabati Daun Mimba Untuk Mengendalikan Hama Pada Tanaman Di Desa Rumpet Kecamatan Krueng Barona Jaya Kabupaten Aceh Besar Arif Sardi; Putri Magfirah
Bumi: Jurnal Hasil Kegiatan Sosialisasi Pengabdian kepada Masyarakat Vol. 2 No. 1 (2024): Januari: Bumi: Jurnal Hasil Kegiatan Sosialisasi Pengabdian kepada Masyarakat
Publisher : Asosiasi Riset Teknik Elektro dan Informatika Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/bumi.v2i1.33

Abstract

This activities aim to provide understanding and skills to the community in Rumpet Village, Krueng Barona Jaya Subdistrict, Aceh Besar Regency, on utilizing local resources to naturally control plant pests. The primary focus of this activity is the process of making botanical pesticides from neem leaves as an environmentally friendly solution to sustain agriculture in the region. The activity begins with a socialization on the importance of sustainable pest control and the adverse effects of chemical pesticides. It involves active participation from the community in collecting and identifying neem leaves as the main ingredient. The process of extracting active compounds from neem leaves is also taught to the community, along with formulation techniques and the application of the resulting botanical pesticide. The outcomes of this activity include an increase in community knowledge about natural plant pest control and their empowerment in producing and using botanical pesticides from neem leaves. This community service is expected to make a positive contribution in promoting sustainable and resilient farming practices in Rumpet Village, inspiring surrounding communities to adopt similar approaches in their agricultural management.
Identifikasi Cendawan Pada Biji Kacang (Vigna Radiata L.) Hijau dengan Menggunakan Metode Blotter Test Sardi, Arif; Wati, Ernia; Hardila, Dwi Intan; Raharjo, Novan Karnanto
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 1 No. 1 (2021): April 2021
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v1i1.798

Abstract

Kacang hijau (Vigna radiata L.) merupakan jenis kacang-kacangan yang paling banyak dikonsumsi oleh masyarakat Indonesia. Produksi kacang hijau yang dicapai petani masih terbilang rendah. Salah satu faktor yang menyebabkan rendahnya hasil produksi kacang hijau adalah serangan penyakit. Serangan penyakit pada biji kacang hijau dapat disebabkan oleh bakteri, virus dan cendawan. Sebagian besar biji kacang hijau disimpan dalam karung yang terikat dan disimpan didalam gudang, maka kondisi suhu yang tidak sesuai akan menyebabkan cendawan tumbuh dan melekat pada biji kacang hijau. Metode yang digunakan pada penelitian ini adalah metode blotter test. Metode blotter test adalah salah satu metode isolasi yang mengkondisikan agar cendawan dapat tumbuh pada kertas saring (blotter). Keuntungan dari metode blotter test yaitu cara pengerjaannya yang praktis dan dapat mengidentifikasi cendawan terbawa benih. Hasil yang didapatkan pada hari ke-7 yaitu cendawan Fusarium sp. dan Rhizoctonia solani.
PENGARUH PEMBERIAN DOSIS PUPUK UREA TERHADAP PERTUMBUHAN TANAMAN KANGKUNG DARAT (Ipomoea reptans Poir) Nanda Vidasari; Arif Sardi; Rosmeri
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 1 No. 2 (2021): Oktober 2021
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v1i2.1918

Abstract

Kangkung merupakan salah satu jenis sayuran yang banyak ditemukan di Indonesia. Kandungan utama yang terdapat di dalam sayur kangkung adalah zat besi yang berguna untuk pertumbuhan dan kesehatan tubuh. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian berbagai dosis pupuk urea terhadap pertumbuhan tanaman kangkung dan mengetahui dosis pupuk urea yang tepat untuk pertumbuhan tanaman kangkung. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan yaitu U0 (Kontrol) = 0 gr urea/ polybag, U1 = 0,5 gr urea/polybag, U2 = 1 gr urea/polybag, dan U3 = 1,5 gr urea/polybag dengan 4 kali pengulangan pada masing masing perlakuan. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang nyata pada pertumbuhan tinggi tanaman dan jumlah helaian daun tanaman kangkung pada dosis U0 (Kontrol) dengan perlakuan U3. Untuk pengamatan pertumbuhan tanaman kangkung pada berbagai dosis pupuk urea didapatkan hasil pengukuran rata-rata pertumbuhan dapat dilihat pada 7 HST untuk perlakuan U0 (Kontrol) didapatkan tinggi batang yaitu 22,8 cm, dan helaian daun 45. Tinggi tanaman pada perlakuan U3 didapatkan hasil tinggi tanaman 21,6 cm dan jumlah helaian daun 50. Pengamatan pertumbuhan tanamn kangkung pada hari ke 29 HST didapatkan hasil rata – rata pertumbuhan tanamn kangkung, pada perlakuan U0 (Kontrol) didapatkan hasil tinggi tanaman 140,08 cm, dan jumlah helaian daun 83,75. Sedangkan pada perlakuan U3 didapatkan hasil rata – ratatinggi tanaman 182,025 cm dan jumlah helaian daun 102,5. Kata kunci: tanaman kangkung, dosis, pupuk urea
ENUMERATION AND PATHOGENICITY TEST OF Vibrio Sp ON Green Mussels (Perna viridis) FROM THE KRUENG CUT AREA OF ACEH BESAR Syafrina Sari Lubis; Arif Sardi; Nailul Muna
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 2 No. 1 (2022): April 2022
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v2i1.1920

Abstract

Kerang hijau (Perna viridis) merupakan jenis biota memiliki kandungan zat gizi yang baik untuk dikonsumsi dan nilai ekonomis. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri Vibrio sp., melakukan enumerasi total koloni dengan metode Total Plate Count dan uji patogenitas Vibrio sp. dengan media Blood Agar plate (BAP). Isolasi dengan menggunakan media selektif Thiosulfate Citrate Bile Salts (TCBS) diperoleh 16 isolat. Hasil uji biokimia TSIA (Triple Sugar Iron Agar), uji MRVP (Methyl Red Voges- Proskauer), uji katalase, uji oksidasi, uji SIM (Sulfide Indole Motility) dan Uji Simmon Citrat, menunjukkan isolat Vibrio sp. memiliki kemampuan yang berbeda dalam menghidrolisis media pengujian. Enumerasi total koloni VP10 dengan jumlah 4,63 x 102 Cfu/g sedangkan hasil terendah yaitu isolat VP14 yang diperoleh dengan TPC adalah 2,36 x 102 Cfu/g.. Hasil pengujian patogenitas 16 isolat bersifat patogen karena memiliki kemampuan melisiskan darah yang terdapat didalam media Blood Agar Plate dan bersifat beta hemolisis.
Co-Authors Abd Mujahid Hamdan Abidah Nur Asman Manaf Diannita Harahap Dwi Intan Hardila Dwi Yuliandhani Epa Paujiah, Epa Ernia Wati Febriyani, Nadila Feizia Huslina Harahap, Diannita Hardila, Dwi Intan Hayatillah, Raudah Hayatillah, Raudhah Ilham Zulfahmi Jamaluddinsyah Jamaluddinsyah Jamaludin MALIK Jamsari Jamsari Khairatul Ulya Khairatul Ulya Khairun Nisah Lina Rahmawati Mahyana Mahyana Maria Maria lisa Maulida, Zahratul ML Edy Parwanto Muliari Muliari Nabilla Munawarah Nabilla Munawarah Nailul Muna Nailul Muna Najiba, Aliya Nanda Vidasari Nanda Vidasari Neri Kautsari Novan Karnanto Raharjo Putri Magfirah R Nurlian R Nurlian Raharjo, Novan Karnanto Reksamunandar, Rhyan Prayuddy Rizky Ahadi Rosmeri Rosmeri Rumondang Rumondang Siti Aisyah Syafrina Sari Lubis Syamsul Arifin Syamsurizal Syamsurizal Tanwirul Anwar Veny Wilya, Veny Wahyuni, Fitrah Wati, Ernia Yanwirasti Yanwirasti Yunina Rahmi Yusrizal Akmal Zulfajriana