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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi KUKILA Jurnal Biologi Universitas Andalas Jurnal Sumberdaya Hayati Aceh Journal of Animal Science AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) Jurnal Riset Biologi dan Aplikasinya Jurnal Pengabdian kepada Masyarakat Nusantara Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Lombok Journal of Science Journal on Biology and Instruction (JouBIns) Jurnal Natural Metamorfosa: Journal of Biological Science Open Access DRIVERset
Janra, Muhammad Nazri
Biology Department, Andalas University
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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DNA Barcoding and eDNA Metabarcoding for Identification Species: A Case Study (West Sumatra): DNA barcoding and eDNA metabarcoding Roesma, Dewi Imelda; Tjong, Djong Hon; Syaifullah, Syaifullah; Nofrita, Nofrita; Janra, Muhammad Nazri; Aidil, Dyta Rabbani; Prawira, Furqan Dwiki Lintang; Salis, Viola Mutiara
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

The biodiversity of freshwater fish is important to study because there is data and information that remain undiscovered. The waters of Sumatra, especially West Sumatra, are areas with high freshwater fish diversity but have limited information. Providing information and genetic data has become one of the important things to conduct. DNA barcoding and eDNA metabarcoding have become molecular methods for identifying species and providing information about the presence of species in a region. A study using DNA barcoding and eDNA metabarcoding was conducted on freshwater fish in several locations in West Sumatra. Isolation and amplification of DNA were performed directly on individual samples and sequenced using conventional methods (Sanger sequencing) to generate DNA barcodes. Water samples were collected (2 liters) at each location using a sterile bottle. The water samples were filtrated, isolated, and amplified using universal primer and sequenced with next-generation sequencing techniques. The study successfully collected 25 species belonging to 14 genera, 2 families, and 1 order. A total of 134 sequences from West Sumatra with a length of 648-670 bp were analyzed. All DNA barcodes were submitted to the BOLD System and GenBank, NCBI. The mean Kimura two-parameter model (K2P) genetic distances within species, genera, families, and orders were 0.7%, 8.3%, 15.8%, and 21.3%, respectively. The eDNA metabarcoding technique has successfully detected three native fish species in the waters of West Sumatra (Barbonymus schwanefeldii, Mystacoleucus padangensis, and Rasbora jacobsoni). The availability of fish DNA barcodes in reference databases is crucial for the success of identification using eDNA metabarcoding. Combining identification using conventional methods and eDNA metabarcoding can provide more reliable results and become a reference for future freshwater monitoring.
Co-Authors . Mansyurdin Aadrean Aadrean Ada Chornelia Ade P Agung Agung, Ade P Ahmad Mursyid Ahmad Mursyid Aidil, Dyta Rabbani Aisyah, Rihadatul Alfajri, Negi Andri Saputra Ashrifurrahman, Ashrifurrahman Azura, Nabilah Rahmachila Bramadi Arya Chornelia, Ada Dahelmi Dahelmi David Gusman Dewi Imelda Roesma Dika Putri Sehati Djong Hon Tjong Eli Ratni Elni Fatimah Eti Yerizel Fajri Hidayat Fandesti, Shania Refka Fauzi, Ibrahim Gusman, David Hafizhah Rahmayani Handika, Heru Henny Herwina HENRY HERWINA Herlina Putri Endah Sari Heru Handika Heru Handika Jabang Nurdin Jasmi Jasmi Jasmi , Jasmi Jasmi Jasmi Jasmi Jasmi Jefrial Santoso Lily Rahmawati Lily Rahmawati, Lily Mairawita, Mairawita Maliza, Rita Meliya Wati meliya wati Miftahul Ilmi Miftahul Ilmi, Miftahul Muhammad Idris Muhammad Idris Mursyid, Ahmad Nabilah Rahmachila Azura Nofrita Nofrita Nofrita Nofrita Pratama Elisa, Tasya Putri Prawira, Furqan Dwiki Lintang Putra Santoso Rahmayani, Hafizhah Rezvi, Syakira Tiara Richard Noske Richard Noske Riski Andrian Jasmi Rusdimansyah Salis, Viola Mutiara Satria, Rijal Sehati, Dika Putri Shania Refka Fandesti Sinurat, Arthauly Yopita Siti Salmah Siti Salmah Suwirmen, Suwirmen Syaifullah Syaifullah Syakira Tiara Rezvi Syamsuardi Syamsuardi Tasman, Ratna Juwita Tofrizal, Alimuddin Wilson Novarino Yeni Gusma Yanti Yundari, Yundari