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Sukarjati
Universitas PGRI Adi Buana Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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DAMPAK INFEKSI Escherichia coil DAN ADHFSIN PILI Escherichia coil ISOLAT SEMEN PRIA INFERTIL BM 32.2 KDA PADA MOTILITAS, VITALITAS DAN MORFOLOGI SPERMATOZOA MARMUT Sukarjati, Sukarjati; Sudjarwo, Sudjarwo
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 4 No 01 (2010)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol4.no1.a1086

Abstract

Penelitian ini bertujuan untuk mengkaji dampak infeksi E. coil dan imunisasi adhesin pill E. coil BM 32.2 kDa terhadap kualitas spermatozoa Marmut. Sampel penelitian ini. adalah spermatozoa dari 15 Marmut. Uji toksisitas adhesin pili E. coil BM 32.2 kDa terhadap kualitas spermatozoa marmut dilakukan dengan mengamati motilitas, vitalitalis dan morfologi sperms marmut imicroskop. Hasil penelitian menunjukkan bahwa tidak ada perbedaan antara marmut kontrol negatif dan marmut perlakuan (yang diimunisasi dengan adhesin pili E. coil BM 32.2 kDa ) pada motilitas spermatozoa (p0.499), vitalitas spermatozoa (p.817) dan morfologi normal spermatozoa (p--3.176). Antara marmut kontrol negatif dan marmut kontrol positif (yang diinfeksi dengan E. coli secara transuretral) ada perbedaan balk pada motilitas (p0.000), vitalitas (p=0.000) maupun morfologi (p=0.000). Antara marmut kontrol positif dan marmut perlakuan ada perbedaan pada motilitas (p-71.001), vitalitas (p=0.000) dan morfologi (p-.000). Hasil pengamatan morfologi menunjukkan bahwa pada marmut yang infeksi secara tranuretral dengan E. coil tampak adanya granulosit dan E. coil yang melekat pada sperms epididimis. Berdasarkan basil penelitian ini dapat disimpulkan bahwa infeksi E. coil secara transuretral berdampak menurunkan kualitas sperm epididimis marmut dan protein adhesin pili E. coil BM 32.2 kDa yang diimtmisasikan ke marmut tidak berpengaruh terhadap kualitas spermatozoa epididimis Marmut.
PENGENALAN SAINS DATA BIOLOGI UNTUK SISWA SMA: MEMBANGUN LITERASI DATA SEJAK DINI DALAM ERA DIGITAL Ajiningrum, Purity Sabila; Diah Karunia Binawati; Pungky Slamet Wisnu Kusuma; Sukarjati; Vivin Andriani; Tatang Sopandi; Arif Yachya
Jurnal Abadimas Adi Buana Vol 9 No 01 (2025): Jurnal Abadimas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/abadimas.v9.i01.a10561

Abstract

Era digital yang berlangsung saat ini membutuhkan kemampuan literasi data sebagai keterampilan penting yang perlu dimiliki oleh generasi muda, termasuk dalam bidang biologi. Pembelajaran biologi di tingkat SMA cenderung berfokus pada hafalan konsep teoritis dan belum banyak menyentuh aspek pengolahan dan analisis data. Padahal, pendekatan sains data dapat membantu siswa memahami fenomena biologis secara lebih kontekstual dan berbasis bukti. Pengabdian kepada masyarakat ini bertujuan untuk mengenalkan konsep dasar sains data biologi kepada siswa SMA melalui pelatihan dan praktik langsung. Siswa akan dilatih untuk mengumpulkan, mengolah, menganalisis dan memvisualisasikan data sederhana dari pengamatan biologi di sekitar mereka, seperti data pertumbuhan tanaman, aktivitas fauna, atau kondisi lingkungan. Dengan bantuan perangkat sederhana seperti Excel atau Google Sheets, siswa diarahkan untuk berpikir analitis dan menyimpulkan informasi dari data nyata. Diharapkan kegiatan ini dapat meningkatkan literasi data siswa, memperkaya metode pembelajaran biologi di sekolah, serta membangun ketertarikan siswa terhadap biologi sebagai ilmu yang dinamis dan aplikatif. Berdasarkan hasil pre-test dan post-test, terjadi peningkatan rata-rata pemahaman siswa sebesar 37,3%, terutama kemampuan menginterpretasikan data, membuat grafik, dan menyusun kesimpulan berbasis bukti. Selain itu, siswa lebih antusias mempelajari biologi secara aplikatif dan menyatakan bahwa pendekatan pembelajaran berbasis data membantu mereka memahami materi biologi secara lebih nyata dan kontekstual .
Tepung Labu Kuning (Cucurbita moschata), Tepung Porang (Amorphophallus muelleri Blume), dan Kombinasi Kedua Tepung sebagai Penurun Gula Darah dan Peningkat Motilitas Spermatozoa Mencit (Mus musculus) Sukarjati, Sukarjati; Evarina, Melinda Yuti; Andriani, Vivin
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8941

Abstract

Diabetes Mellitus (DM) is a global epidemic with high morbidity and mortality. Diabetes Mellitus (DM) is thought to have a negative impact on sperm quality. Yellow pumpkin contains antioxidants that can neutralize free radicals which can improve fertility. Glucomannan is a substance contained in porang which functions as a soluble fiber and plays a role in blood glucose balance. This study aims to examine pumpkin flour, porang flour and a combination of both flours on sperm motility and body weight of alloxan-induced diabetic mice. This study used 25 mice aged 3 months, body weight 25gr-30 gr. This study used a completely randomized plan (CRD) with 5 replications and 5 treatments. The treatments were negative control (diabetic mice), positive control (administration of glimepiride at a dose of 0.0078 mg/mice per day), D1 (pumpkin flour 134.4 mg/30g BW of mice), D2 (porang flour 63mg/30g BW mice), D3 (combination 50% : 50% = 67.2 mg : 31.5 mg/30 grBW mice). Diabetic mice were made by intraperitoneal induction of alloxan dose of 200 mg/Kg for 3 days. The parameters observed were spermatozoa motility, body weight and blood sugar levels of the mice. The research data were analyzed using the ANOVA test followed by the BNT test. The results showed that the administration of glymeride, pumpkin flour, porang flour and their combination had a significant effect on reducing blood sugar (p=0.000) and sperm motility (p=0.000), but had no effect on body weight (p=0.317). Conclusion: pumpkin flour, porang flour or a combination of both flours can be used as herbs to lower blood sugar and increase sperm motility in diabetes sufferers.
Leaf and Petiole Extract of Centella Asiatica are Potential for Antifertility and Antimicrobial Material Amilah, Susie; Sukarjati, Sukarjati; Rachmatin, Diaz P; Masruroh, Masruroh
Folia Medica Indonesiana Vol. 55, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Ethanol extract of leaves and petiole of pegagan (Centella asiatica) is potential as antimicrobial and antifertility. This study was to investigate whether ethanol extracts of leaf and petiole of Centella asiatica has potential as an antimicrobial and can decrease sperm quality and spermatogenic cells of mice. This was a randomized controlled study using Centella asiatica plant from which we made ethanol extract. The extract of the leaves and petiole of Centella asiatica was subjected to antimicrobial test using the bacteria S. aureus, S. epidermidis, and A. aerogenes. Then the observation was done after a 24-hour-old bacterial cultures by measuring the diameter of the clear zone in mm. Thereafter, the extract was given to randomly grouped mice that were divided into two groups receiving the leaf extract in one group and the petiole exract in another for 35 days. On day 36 the mice were sacrificed for spermatozoa quality and testicular histology examination. Statistical analyses used in this study were ANOVA and LSD tests. There was inhibition zone difference between leaf and petiole (p=0.000). Inhibition zone between bacteria S. epidermidis, S. aureus and A. aerogenes showed no difference (p=0.198). Inter-dose inhibition zone showed difference (p=0.000). Centella asiatica leaf extract showed inhibition zone better than that of Centella asiatica petiole extract. In spermatozoa quality test, there was effect of Centella asiatica leaf extract on sperm motility (p<0.05), viability (p<0.05), morphology (p<0.05) and concentration (p<0.05). No effect of pegagan petiole extract was found on sperm motility (p<0.05), viability (p<0.05), abnormal morphology (p<0.05) and concentration (p<0.05). Highest degradation of spermatozoa quality was obtained from Centella asiatica petiole extract at a concentration of 150 mg/kg bw. Spermatogenic cell test results showed no effect of Centella asiatica leaf extract in decreasing spermatogonia (p=0.000), spermatocytes (p=0.000), and spermatids (p=0.000) counts. No effect of pegagan petiole extract in decreasing the number of spermatogonia, spermatocytes and spermatids. The highest reduction spermatogenic cells of seminiferous tubules was obtained by Centella asiatica petiole extract in a concentration of 150 mg/kg bw. In conclusion: Centella asiatica leaf and petiole extract has antimicrobial potency as contra-ceptives
Role of immunoglobulin g (igg) from the induction of escherichia coli pili adhesion protein isolated from infertile male semen with 32.2 kda molecular weight as opsonin and anti-adhesion an in vitro escherichia coli infection Sukarjati, Sukarjati; Amilah, Susie
Folia Medica Indonesiana Vol. 53, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Escherichia coli (E. coli), a major cause of male genital tract infections, is asymptomatic and may result in male infertility. We have succeeded in isolating and characterizing proteins of E. Pili coli isolates from semen of infertile men who function as adhesin with a molecular weight (MW) 32.2 kDa. This study aims to prove the ability of IgG results adhesion proteins induced pili of E. MW coli 32.2 kDa as opsonin to determine the value of the activity and phagocytic capacity and as an anti- adhesion by calculating the average number of E. coli that attached to human spermatozoa. E. coli infertile men's semen were cultured using standard bacteriology. Peritoneal macrophages were isolated from mice. Spermatozoa from donors were prepared using Sil with Select Plus. IgG was obtained from mice immunized with (1) PBS (control), (2) E. coli pili adhesion protein isolated from infertile men semen with MW of 32.2 kDa and (3) weakened E. coli isolated from infertile men's semen. Phagocytic activity value was determined by counting the number of cells activated macrophage phagocytosis process in 100 cells. Phagocytic capacity value was determined by counting the number of bacteria ingested by 25 macrophages. Anti-adhesion test was done by counting the number of bacteria attached to 100 spermatozoa. The results of this study showed difference (p=0.000) in phagocytic activity and phagocytic capacity (p=0.000) between treatment (1) and (2), and between treatment (1) to (3). However, treatment (2) and (3) did not differ neither in phagocytic activity (p=0.693) nor in phagocytosis capacity (p=0.125). Anti-adhesion test produces difference (p=0.000) in the number of E. coli that attached to human spermatozoa between treatments (1) and treatment (2), and between treatments (1) and (3). The number of E. coli that attached to human spermatozoa between treatment (2) and treatment (3) was not significantly different (p=0.371). In conclusion, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa can increase phagocytic activity and capacity as well as serve as an anti- adhesion. Thus, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa is protective against in vitro E. coli infection, so that it can be used as material to prevent male reproductive tract infections due to E. coli.
Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System Sukarjati, Sukarjati; Amilah, Susie; Sudjarwo, Sudjarwo
Folia Medica Indonesiana Vol. 54, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Escherichia coli (E. coli) is the leading cause of male genital tract infection with no symptoms of infertility. Protein E. coli pili hemagglutinin isolated from infertile male sperm with 32.2 kDa MW acts as adhesion in spermatozoa. This study aimed to prove whether E. coli pili adhesin 32.2 kDa MW is toxic to male reproductive system. Samples consisted of spermatozoa of 30 guinea pigs divided into three groups: control, immunized with E. coli pili adhesin 32.2 kDa MW protein, and transurethral infected E. coli. Observations of sperm motility, vitality and morphology were performed under a microscope. MDA levels and sperm DNA damage were measured by a spectrophotometer and comet assay method and observed using a fluorescent microscope. There was no difference between control and immunization group of E. coli pili adhesin in motility (p=0.499), vitality (p=0.817) and morphology (p=0.176); between control and transuretral infection groups in motility (p=0.000), vitality (p=0.000) and morphology (p=0.000); and between control and both treatment groups in motility (p=0.001), vitality (p=0,000) and morphology (p=0.000). Histologic analysis showed E. coli pili adhesin of 32.2 kDa MW immunization group did not suffer from testicular tissue damage, while the positive group showed a deterioration of seminiferous tubular cells. MDA levels differed between immunization group E. coli pili, transurethral infection group, and control (p=0.024) and between transurethral and control (p=0.007) groups. However, between control and immunized group with E. coli pili protein showed no difference (p=0.251). DNA damage differed (p=0.000) between immunized group with E. coli pili, transurethral infection and control group; between control and transurethral infected group (p=0.000); and between transurethral infection group and E. coli pili protein immunization group (p=0.000). However, between control and E. coli pili immunization group showed no difference (p=0.600). In conclusion, E. coli pili adhesin 32.2 kDa MW protein is not toxic for sperm quality and the quality of sperm molecules.
PEMBERDAYAAN MASYARAKAT MELALUI INOVASI PRODUK MIE DAUN KELOR SEBAGAI PANGAN FUNGSIONAL BERGIZI TINGGI Ajiningrum, Purity Sabila; Arif Yachya; Tatang Sopandi; Sukarjati; Vivin Andriani; Pungky Slamet Wisnu Kusuma; Diah Karunia Binawati
Jurnal Abadimas Adi Buana Vol 9 No 02 (2026): Jurnal Abadimas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/abadimas.v9.i02.a10808

Abstract

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan ibu-ibu PKK Desa Janti, Kabupaten Sidoarjo dalam mengolah daun kelor menjadi produk pangan sehat berupa mie daun kelor. Kelor (Moringa oleifera) merupakan tanaman lokal yang kaya zat gizi dan berpotensi sebagai bahan pangan fungsional. Pelatihan dilaksanakan pada bulan Agustus–September 2025 dan diikuti oleh 20 peserta. Metode pelaksanaan meliputi tahapan persiapan, sosialisasi, pelatihan, serta evaluasi pengetahuan melalui pre-test dan post-test. Hasil evaluasi menunjukkan adanya peningkatan nilai rata-rata peserta dari 56,4 pada pre-test menjadi 87,2 pada post-test, atau terjadi peningkatan sebesar 54,6%. Peningkatan tertinggi terjadi pada aspek keterampilan pembuatan mie daun kelor. Kegiatan ini terbukti efektif dalam meningkatkan literasi gizi, keterampilan pengolahan pangan, serta kesadaran akan peluang usaha berbasis bahan lokal, sehingga pelatihan ini dapat menjadi model pemberdayaan masyarakat desa dalam mendukung ketahanan pangan keluarga dan ekonomi rumah tangga.
Potential of Combined Insulin Leaf (Smallanthus sonchifolius) and Noni Fruit (Morinda citrifolia L.) Extracts in Reducing Blood Glucose Level and Spermatogenic Cells Improvement in Diabetic Mice Sukarjati; Aldi Syahputra
Jurnal Penelitian Pendidikan IPA Vol 11 No 5 (2025): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i5.11174

Abstract

Elevated blood glucose levels are a hallmark of diabetes mellitus, which can lead to reproductive disorders, particularly affecting spermatogenic cells. This study aimed to evaluate the effectiveness of insulin leaf extract, noni fruit extract, and their combinations in reducing blood glucose levels and their impact on the number of spermatogenic cells in diabetic mice. A total of 36 male mice were divided into 12 treatment groups, including a normal control group, a negative control group (induced with alloxan 120 mg/KgBW), a positive control group (alloxan + metformin), and nine treatment groups with various doses of individual and combined extracts. Blood glucose levels were measured using Accu-Chek, and testicular histology was prepared following laboratory standards. Data were analyzed using the ANOVA test followed by the Duncan post hoc test. Results showed that the combination of insulin leaf extract at 150 mg/KgBW/day and noni fruit extract at 62.5 mg/KgBW/day was the most effective in reducing blood glucose levels. Additionally, the combination of 125 mg/KgBW insulin leaf extract and 50 mg/KgBW noni fruit extract significantly increased the number of spermatogenic cells. Thus, the combination of insulin leaf and noni fruit extracts shows potential as a natural antidiabetic therapy and for restoring spermatogenic cell function in diabetic conditions.
Potential of Combined Curcuma zedoaria and Piper crocatum Leaf Extracts as Natural Male Antifertility Agents: an Experimental Study in Mice Sukarjati; Mitha Novia Sari
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12380

Abstract

Curcuma zedoaria and Piper crocatum are indigenous Indonesian herbal plants containing various bioactive compounds. C. zedoaria is known to contain curcumin, tannins, saponins, and flavonoids, while P. crocatum contains piperine, tannins, saponins, flavonoids, alkaloids, and triterpenoids. This study aimed to investigate the effects of white turmeric extract, red betel leaf extract, and their combination on the spermatogenic cells of male mice. Extracts were obtained using the maceration method. A total of 30 male mice, aged 3 months and weighing 25–30 grams, were randomly divided into four treatment groups with three replications each: control, white turmeric extract, red betel extract, and a combination of both extracts. The extracts were administered orally at doses of 50, 100, and 150 mg/kg BW for individual extracts, and 25:25, 50:50, and 75:75 mg/kg BW for the combined treatment, over 35 days. Histological sections of the testis were prepared following standard laboratory protocols. One-way ANOVA showed a significant decrease (p < 0.05) in all cell types in treated groups, with the most substantial reduction observed at the 75:75 mg/kg BW combination dose. The conclusion of this study is that the combined extract of C. zedoaria and P. crocatum has the potential as an antifertility
Efek Protektif Ekstrak Jahe Merah (Zingiber officinale var. rubrum) Terhadap Sel Spermatogenik, dan Sel Leydig pada Mencit yang Diinfeksi Staphylococcus aureus secara Intra Uretra Sukarjati; Nur Asiah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 19 No 01 (2026)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.19.01.11432.%p

Abstract

Infections of the male genital tract can cause infertility in up to 15% of cases. Staphylococcus aureus (S. aureus) is a pathogenic bacterium that can significantly contribute to infertility. Red ginger (Zingiber officinale var. rubrum) contains active compounds such as gingerol, shogaol, zingerone, and flavonoids, which have the potential to exert protective effects against testicular tissue damage caused by infection. This study aimed to evaluate the effect of red ginger on spermatogenic cells in mice infected intraurethrally with S. aureus. A total of 30 male mice aged 3 months were used and divided into three groups, with 10 mice in each group: A; control group, B; group infected intraurethrally with S. aureus, and C; group infected with S. aureus and treated with red ginger extract. The results showed a significant effect (p ≤ 0.05) of red ginger extract administration on the number of spermatogonia, spermatocytes, spermatids, Leydig cells, and testicular weight in mice infected with S. aureus intraurethrally. Therefore, red ginger extract can inhibit the growth of S. aureus and improve spermatogenic cell condition in mice affected by S. aureus infection.   Keywords: S.aureus; genital tract infection, spermatogenic cells, red ginger (Zingiber officinale   var. rubrum)
Co-Authors ., Intashofal Ainuris Zakiyah Aldi Syahputra Alfian Dwi Cipta Yani Apolonia M Arif Yachya Asti Rahayu Astuti Dewi Aucky Hinting Aulani F. Fithroh Aulani F. Fithroh D. Febrianti DesyaTresty Medistriani Dewanti, Felicitas Deru Dewi, Astuti Diah Karunia Binawati Diaz P Rachmatin Doddy M. Soebady Dyah Hariani Eka p F Erni Widyaswati Ersanto ersanto ersanto, Ersanto Ervina Ervina Evarina, Melinda Yuti F, Eka p Febrianti Febrianti Febrianti Febrianti Febrianti, D. Febrianti, D. Feri Wiranata Ganda Adi Nugroho Gusti Azzahra Widiaura Hamdani Lunardhi Hasanah, Waqiatul Hidayati, Isnainy Hidayati, Isnainy I.A.K. Pramushinta IAK Pramushinta Isnainy Hidayati Laia, Sanima M, Apolonia M, Apolonia M. Saftian Andreanto Masruroh MASRUROH Masruroh Masruroh Melinda Yuti Evarina Mitha Novia Sari Nanis Choirunnisa Ngadiani Nora Augustien Nova Triani Novi Dwi Kusuma Nugroho , Ganda Adi Nur Asiah Nur Syamsiatul Fajar Nurul Islam Fi Holki Pratama, Yoga Bagas Pungky Slamet Wisnu Kusuma Pungky Slamet WK Pungky Slamet WK Pungky SWK Purity Sabila Ajiningrum R. T. Wahyudi Rachmatin, Diaz P Rahayu, Asti Rahayu Rojib Rojib Rojib, Rojib Rojib, Rojib Sanima Laia Sudjarwo Sudjarwo Sudjarwo Sulistyowati Sulistyowati Susie Amilah Tatang Sopandi Tony Susilo Wibowo Umi Nurhayati Umi Nurhayati V.A. Ferandra Vivin Andriani Wahyudi, R. T. Wahyudi, R. T. Waqiatul Hasanah WK, Pungky Slamet Yoga Bagas Pratama