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Sukarjati
Universitas PGRI Adi Buana Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Novel Therapeutic Potential of 'Ambon' Banana and Green Bean Sprout Extracts in Mitigating Cigarette Smoke-Induced Testicular Oxidative Stress and Sperm Quality Impairment Sukarjati Sukarjati; Alfian Dwi Cipta Yani; Novi Dwi Kusuma; Feri Wiranata
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.5755

Abstract

Cigarette smoke exposure generates free radicals, causing oxidative stress and adversely affecting reproductive organs, resulting in reduced sperm quality. The aim of this study was to investigate the therapeutic benefits of 'Ambon' banana and green bean sprout extracts, either individually or in combination, in mitigating testicular MDA levels and preserving spermatogenic cells in rats exposed to cigarette smoke. 35 Male rats, aged 3 months, were divided into five groups: negative control, positive control (cigarette smoke-exposed), P1 (cigarette smoke + 'Ambon' banana extract), P2 (cigarette smoke + green bean sprout extract), and P3 (cigarette smoke + 'Ambon' banana and green bean sprout extracts). Cigarette smoke exposure was administered at 4 sticks/day/group for 30 days, and extracts were orally administered using a sonde for the same duration. The study showed that 'Ambon' banana extract, green bean sprout extract, and their combination significantly reduced testicular MDA levels, increased the number of spermatogonia, spermatocytes, spermatids, testicular weight, diameter of seminiferous tubules, and the number of mouse Leydig cells in rats. This study highlights the potential therapeutic effects of 'Ambon' banana and green bean sprout extracts as protective agents against cigarette smoke-induced testicular oxidative stress and sperm quality impairment.
Enhancing Spermatozoa Quality and Spermatogenic Cell in Hyperglycemic Mice (Mus musculus L.) with Sambung Nyawa (Gynura procumbens) and Mahagany (Swietenia mahagoni jacq) extracts, Alone and in Combination Sukarjati; Umi Nurhayati; DesyaTresty Medistriani
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7180

Abstract

This study aimed to investigate the effect of the ethanol extract of sambung nyawa (Gynura procumbens) leaves, mahogany (Swietenia mahagoni jacq) seeds, and their combination on the motility, viability, morphology, and concentration of spermatozoa, spermatogonia, spermatocytes, and spermatids in hyperglycemic mice induced with 10% dextrose. A total of 48 male mice were used as experimental animals, and the extracts were administered orally for 35 days. On day 36, spermatozoa and testicular tissues were collected for analysis. The results showed that the extracts at various doses had the potential to reduce blood glucose levels and improve the motility, viability, morphology, and concentration of spermatozoa and germ cells in hyperglycemic mice. Result:The combination of sambung nyawa leaves and mahogany seeds at a dose of 150:150 mg/kg BW was found to be the most effective in reducing blood sugar levels and improving sperm parameters. Therefore, these extracts have the potential to be used as alternative herbal ingredients with similar effects to metformin in reducing diabetes and improving fertility.
The Effect of Potassium Dosage and Branch-Pruning on the Growth and Yield of Butternut Squash (Cucurbita moschata Durch) F. Deru Dewanti; Nora Augustien; Nova Triani; Nurul Islam Fi Holki; Sukarjati
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.7565

Abstract

Butternut squash (C. moschata) is a honey squash plant (Cucurbita moschata Durch) which is an introduced plant from the lowlands of South America and has adapted to Indonesia. The research was carried out in the planting area of Kampung Labu Madu, Toyoresmi Village, Ngasem District, Kediri Regency from January to June 2021. Potassium is among major nutrients needed by plants in their growth and development.  Potassium fertilization can help plants improve the regulation of photosynthetic mechanisms, carbohydrate translocation, protein synthesis This research was a two-factor factorial experiment prepared using a Split Plot Design. The first factor (main plot) was the branch pruning (P) treatment which consisted of 2 levels and the second factor (subplot) was the dose of KCl (K) fertilizer which consisted of 4 levels. The results of the research showed that there was a real influence in the treatment without pruning on the parameters of the number of male flowers and on the parameter of fruit weight with the best treatment being pruning, whereas for the treatment the dose of KCl fertilizer had a real effect on the parameters.
The Impact of Smoking on Sperm Quality and the DAZ Gene Sukarjati; Nanis Choirunnisa; Nur Syamsiatul Fajar
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.9213

Abstract

Indonesia's male population is mostly smokers. The chemical content in cigarettes can reduce the quality of spermatogenesis, where spermatogenesis is influenced by the DAZ gene (deleted in Azoospermia). This study aims to analyze sperm quality in light, moderate, and heavy smokers in 3 age groups and analyze the presence of the DAZ gene in heavy smokers. This study used 180 samples divided into three age groups, namely men aged 26-30 years, 31-35 years, and 36-40 years. Each age group recorded the number of cigarettes consumed per day (light, moderate, and heavy smokers) and examined the quality of sperm. While the DAZ gene analysis used 10 samples of heavy male smokers, aged 25-40 years. Sperm quality analysis was based on the WHO laboratory manual for human semen testing, while DAZ gene analysis used the PCR method. Sperm quality data were analyzed using Kruskal-Wallis, while the DAZ gene was analyzed qualitatively descriptively by analyzing electrophoresis photos. The results of this study can be concluded that daily cigarette consumption in the age group affects sperm quality and in heavy smokers, there are 10% who experience deletions in the DAZ gene. Thus cigarettes can reduce spermatozoa quality.
Co-Authors ., Intashofal Ainuris Zakiyah Aldi Syahputra Alfian Dwi Cipta Yani Apolonia M Arif Yachya Asti Rahayu Astuti Dewi Aucky Hinting Aulani F. Fithroh Aulani F. Fithroh D. Febrianti DesyaTresty Medistriani Dewanti, Felicitas Deru Dewi, Astuti Diah Karunia Binawati Diaz P Rachmatin Doddy M. Soebady Dyah Hariani Eka p F Erni Widyaswati Ersanto ersanto ersanto, Ersanto Ervina Ervina Evarina, Melinda Yuti F, Eka p Febrianti Febrianti Febrianti Febrianti Febrianti, D. Febrianti, D. Feri Wiranata Ganda Adi Nugroho Gusti Azzahra Widiaura Hamdani Lunardhi Hasanah, Waqiatul Hidayati, Isnainy Hidayati, Isnainy I.A.K. Pramushinta IAK Pramushinta Isnainy Hidayati Laia, Sanima M, Apolonia M, Apolonia M. Saftian Andreanto Masruroh Masruroh Masruroh MASRUROH Melinda Yuti Evarina Mitha Novia Sari Nanis Choirunnisa Ngadiani Nora Augustien Nova Triani Novi Dwi Kusuma Nugroho , Ganda Adi Nur Asiah Nur Syamsiatul Fajar Nurul Islam Fi Holki Pratama, Yoga Bagas Pungky Slamet Wisnu Kusuma Pungky Slamet WK Pungky Slamet WK Pungky SWK Purity Sabila Ajiningrum R. T. Wahyudi Rachmatin, Diaz P Rahayu, Asti Rahayu Rojib Rojib Rojib, Rojib Rojib, Rojib Sanima Laia Sudjarwo Sudjarwo Sudjarwo Sulistyowati Sulistyowati Susie Amilah Tatang Sopandi Tony Susilo Wibowo Umi Nurhayati Umi Nurhayati V.A. Ferandra Vivin Andriani Wahyudi, R. T. Wahyudi, R. T. Waqiatul Hasanah WK, Pungky Slamet Yoga Bagas Pratama