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All Journal HAYATI Journal of Biosciences Journal of Multidisciplinary Applied Natural Science Sewagati
Firli Rahmah Primula Dewi
Department Of Biology, Faculty Of Science And Technology, Universitas Airlangga, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Other

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Journal : Journal of Multidisciplinary Applied Natural Science

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Antioxidant and Anticancer Effects of Red Okra (Abelmoschus esculentus L.) Ethanol Extract through In Vitro and In Vivo Colorectal Cancer Models Wahyuningsih, Sri Puji Astuti; Mamuaya, Brigita Klara Krisdina; Dewi, Firli Rahmah Primula; Hapsari, Lukiteswari Dyah Tri; Kusuma, Baskara Wiku Adi; Nurhayati, Awik Puji Dyah
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.367

Abstract

Colorectal cancer (CRC) remains the second leading cause of cancer-related mortality worldwide. Affordable herbal sources such as red okra (Abelmoschus esculentus L.) pods have gained attention as potential alternative therapies for CRC. This study aimed to evaluate the antioxidant and anticancer effects of red okra ethanol extract (ROE) using both in vitro and in vivo colorectal cancer models. The antioxidant activity of ROE was assessed using the DPPH assay, while cytotoxic activity was evaluated using the MTT assay on SW480 and HCT116 cell lines. An in vivo study was conducted using rats divided into six groups: normal control, negative control (MNU 10 mg/kg BW), positive control (MNU + methotrexate 0.08 mg/kg BW), and treatment groups receiving MNU combined with ROE at doses of 50, 100, and 200 mg/kg BW for 28 days. Serum levels of Bcl-2, COX-2, VEGF, and MMP-9 were analyzed, and histopathological evaluations of colon tissues were performed. Data were statistically analyzed using one-way ANOVA followed by Duncan’s post hoc test. Statistical significance was determined at p<0.05. ROE exhibited potent antioxidant activity (IC₅₀ = 59.66 ppm) and induced cytotoxic effects by reducing SW480 cell growth and inhibiting HCT116 cell proliferation. Moreover, ROE significantly decreased the expression of Bcl-2, COX-2, VEGF, and MMP-9. These biomarkers are associated with apoptosis inhibition, angiogenesis, inflammation, and metastasis, respectively. Histopathological analysis confirming reduced inflammatory infiltration and suppression of colon carcinogenesis. The optimal in vivo dose was 50 mg/kg BW. These findings support the development of ROE as a promising natural agent for colorectal cancer prevention and therapy.
Dual Antifungal and Immunoregulatory Actions of Topical Silver Nanoparticles from Piper ornatum Extract in Cutaneous Candida albicans Infection Dewi, Firli Rahmah Primula; `Mustofa, Laila Al Azizi; Hamdin, Candra Dwipayana; Geraldi, Almando; Lim, Vuanghao; Pramudya, Manikya; Hayati, Alfiah; Hajar, Versa Rachmania
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.374

Abstract

Cutaneous Candida albicans infection is characterized by persistent inflammation, epidermal damage, and dysregulated immune responses. Silver nanoparticles (AgNPs) have emerged as promising antifungal agents with additional immunomodulatory properties; however, their effects on skin pathology and local immune responses during candidiasis remain incompletely defined. This study aimed to investigate the therapeutic effects of a topical AgNP-based cream in a murine model of C. albicans–induced skin infection. AgNPs were green-synthesized using an aqueous leaf extract of Piper ornatum. A murine model of cutaneous C. albicans infection was established, and infected mice were treated topically with AgNP-based cream formulations at concentrations of 4% or 6%. Disease severity was assessed through macroscopic skin evaluation and histopathological analysis. Immune modulation was examined by flow cytometric analysis of CD4⁺ T-cell subsets expressing TNFα and IL-17, as well as CD11b⁺ myeloid cells expressing IL-6 and IL-10. Untreated infected mice exhibited severe cutaneous pathology, including persistent erythema, erosive lesions, epidermal hyperkeratosis, and acanthosis. These changes were accompanied by marked immune dysregulation, characterized by expansion of CD4⁺IL-17⁺ T-cells, suppression of TNFα-producing CD4⁺ T-cells, increased IL-6 expression, and reduced IL-10 production in CD11b⁺ myeloid cells. Topical AgNP treatment significantly ameliorated macroscopic and histological skin damage, restoring epidermal architecture toward normal. Immunologically, AgNP therapy attenuated pathological Th17 responses, reduced IL-6-producing myeloid cells, enhanced IL-10 expression, and maintained TNFα at controlled levels. Both AgNP concentrations were effective, with the 4% AgNP formulation showing slightly superior normalization of epidermal thickness and inflammatory markers. Overall, topical AgNP-based cream effectively alleviated cutaneous C. albicans infection by combining antifungal activity with coordinated immunomodulation of both adaptive and innate immune responses. By suppressing excessive IL-17– and IL-6–driven inflammation while promoting regulatory immune pathways, AgNP treatment supports tissue repair and immune homeostasis, highlighting its potential as a therapeutic strategy for cutaneous candidiasis.
Co-Authors Achhlam, Divany Hunaimatul Adelah Putri Milenia Sari Agus Supriyanto Alfiah Hayati Almando Geraldi Anjar Tri Wibowo Awik Puji Dyah Nurhayati Dwi Kusuma Wahyuni Hajar, Versa Rachmania Hamdin, Candra Dwipayana Hapsari, Lukiteswari Dyah Tri Hari Soepriandono Intan Ayu Pratiwi, Intan Ayu Junairiah Junairiah Kusuma, Baskara Wiku Adi Lim, Vuanghao Mamuaya, Brigita Klara Krisdina Manikya Pramudya Muhammad Hilman Fu'adil Amin Na'ilah Insani Alifiyah Prenggono, Muhamad Darwin Rasyidah Fauzia Ahmar Sri Puji Astuti Wahyuningsih Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Umi Nur Alfiah Ummi Zubaidah Vuanghao Lim Vuanghao Lim `Mustofa, Laila Al Azizi