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All Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Mutiara Medika: Jurnal Kedokteran dan Kesehatan Jurnal Kesehatan Gigi Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Jurnal Gizi Klinik Indonesia Journal of Community Empowerment for Health Prosiding Seminar Nasional Program Pengabdian Masyarakat Formosa Journal of Sustainable Research (FJSR) Indonesian Journal of Society Development (IJSD)
Ana Medawati, Ana
Fakultas Kedokteran Gigi Universitas Muhammadiyah Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Social Sciences

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Antisense oligonucleotide p45Skp-2 suppresses migratory chemotactic and metastasis of oral malignant Burkitt’s lymphoma cell through down-regulation of MTA-1 and induction of E-cadherin mechanism Supriatno, Supriatno; Irnawati, Dyah; Medawati, Ana
Padjadjaran Journal of Dentistry Vol 32, No 3 (2020): November 2020
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no3.21756

Abstract

Introduction: Burkitt’s lymphoma is a high-grade B-cell neoplasm and one of the most aggressive malignancies of lymphoid origins which found mainly in the paediatric population. The treatment options of this tumour are still limited. However, a new strategy for refractory tumour, phosphorothioate oligonucleotide antisense technique has watched with keen interest. This study was aimed to examine the effect of antisense p45Skp-2 (Skp-2 AS) suppressed migratory chemotactic and metastasis of oral malignant Burkitt’s lymphoma (Raji) cell through down-regulation of MTA-1 and E-cadherin. Methods: True experiment laboratory with post-test control group design was confirmed in this study. The efficiency of Skp-2 AS in the suppression of cell chemotactic migration was examined by Boyden chamber assay. To evaluate the inhibition of cell metastasis was conducted by decreasing MTA-1 expression protein. The expressions of MTA-1, E-cadherin and α-tubulin protein were investigated by Western blot analysis. Results: The results revealed that the number of chemotactic migration of Skp-2 AS treated Raji cell was significantly decreased when compared with that of sense p45Skp-2 (Skp-2 S) and scrambled control (SC) cells (P<0.05) followed by decreased expressions of MTA-1 protein and overexpression of E-cadherin. Interestingly, the expression of α-tubulin protein as an internal control was approximately similar in each transfectant cells. Conclusion: p45Skp-2 have an antitumor activity via suppression of migratory chemotactic activity and metastasis on oral Burkitt’s lymphoma cells through down-regulation of MTA-1 and induction of E-cadherin proteins targeting this molecule could represent a promising new therapeutic approach for this type of cancer.
Co-Authors Adi Saputra, Septian Afiyah, Lisa Lamusul Afiyah, Lisa Lamusul Ahmad Baihaqi Fanani Hidayat Amira Sekar Amalia Andy Rahman Arifta, Arum Nur Atiek Driana Rahmawati, Atiek Azzam Bilal Abdul Aziz Dewi, Sukuriyati Susilo Dhea Aleyda Muthmainnah Dila Gustiana Handayani Dyah Irnawati Ednur, Mutiara Putri Erlangga Narotama Quddus Erlina Sih Mahanani, Erlina Sih Femilian, Afryla Fuad Fatkhurrohman, Fuad Gina Arfianti Putri, Gina Arfianti Hamam Hadi Hamam Hadi Hastami Retriasih Himmaturojuli Rosyid Ridlo Himmaturojuli Rosyid Ridlo, Himmaturojuli I Dewa Putu Pramantara I Dewa Putu Pramantara Ika Andriani Ika Andriani, Ika Keri Pangesti Yudi Harhari Keri Pangesti Yudi Harhari, Keri Pangesti Yudi Khairunnisa, Putri Nabilla Lestra, Citra Maulida Nurhasanah Muhammad Ihza Humanindito Myrna Kania Utami Najma Aliyah Nurhidayah Najmah Aliyah Nurhidayah Nanik Hidayati Normayanti Sukma Normayanti Sukma, Normayanti Nur Arifta, Arum Octavia, Alfini Perdana, Lutfi Putra Retriasih, Hastami Rizka Fakhriani Salsabila, Millati Saputro, Septian Adi Sriyadi Sriyadi Supriatno Supriatno Supriatno Supriatno Supriatno Umar Abdul Zakiyana, Yulida