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THE INFLUENCE OF Cu CONCENTRATION ON Cu-ZEOLITE TO ANTIBACTERIAL POWER IN STREPTOCOCCUS MUTANS Irnawati, Dyah; Agustiono, Purwanto; Wardhani, Endi Hanifah
Jurnal Zeolit Indonesia Vol 9, No 2 (2010)
Publisher : Jurnal Zeolit Indonesia

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Abstract

Microorganisms on the mouth can cause disease on hard and soft muscular of the mouth. One of the microorganisms is a bacteria S. mutans which become etiology of dental caries. The copper (Cu) is one of chemical agents to control microorganism growth. The copper could be combined with zeolite as antibacterial materials. This study aimed to known the affect Cu concentration on Cu-Natural zeolite to antibacterial power of S. mutans. The study was conducted by making five groups of Cu-Zeolite are 0,05M, 0,1M, 0,15M, 0,2M, and 0,25M. 100 mesh zeolite powder was activated at temperature 200 oC during one hours. CuCl2 solution with concentration of 0,05 M until 0,25 M are made each with volume of 80 mL. Cu-zeolite made by reacting 2 gram of zeolite powder with 80 ml of CuCl2 solution for one hours at temperature of 100 oC, then filtered, washed, and dried on the oven at 100 oC during 24 hours. S. mutans bacterial (0,1 mL x 108 CFU/mL) were grown in solid media MHA, then Cu Zeolite (30 mg) was included on pitting at gel media an incubated for 24 hours at temperature 37 oC (n=5). Transulent zone diameter was measured with digital slide-term (0.01 mm). The data were analyzed by anava one stripe and HSH with level signification of 0,05. The result showed an average of translucent zones was 0 mm (0,05 M gropus), 16,03 + 0,47 mm (0,10M), 16,45 + 0,91 mm (0,15M), 18,08 + 0,39 mm (0,20M), and 18,26 + 0,68 mm (0,25M). Anava test showed Cu concentration on 0,10 M up to 0,25 M of Cu Natural zeolite was significantly effect on translucent zones of S. mutans (p< 0,01). The result of HSD showed a significantly difference among all gropus, except on 0,01 M and O,15 M, and 0,20 m and 0,25 m (p>0,05). Conclusion of this study is Cu concentration in Cu-natural zeolite has affected antibacterial power of S. mutans. 0,01 M of Cuconcentration in Cu-natural zeolite has an antibacterial power to S. mutans and highest antibacterial power was on concentration groups of 0,20M.
Effect of Copper (II) Chloride Concentration towards Copper Ions Sorption on Natural Zeolite from Wonosari Irnawati, Dyah; Widjijono, Widjijono; Wijaya, Karna; Asmara, Widya
The Indonesian Journal of Dental Research Proceeding Book
Publisher : The Indonesian Journal of Dental Research

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Abstract

Copper-containing zeolite has been developed as an antibacterial material. It is obtained by reacting zeolite and salt solution with varied concentration of copper. Natural zeolite is abundant in Wonosari, Yogyakarta. The aim of this research was to investigate the concentration effect of copper (II) chloride solutions towards copper ions sorption on natural zeolite from Wonosari. Copper (II) chloride dihydrate powders, CuCl2. 2H2O, (Merck, Germany) and natural zeolite (Wonosari, Indonesia) were used. Zeolite powder (100 mesh) was washed and dried (200oC/2 hours). Copper (II) chloride solution with different concentrations (0.05 M, 0.10 M, 0.15 M, 0.20 M, and 0.25 M) were prepared. Zeolite and CuCl2 solutions (50 g/250 ml) were reacted at 60oC for 1 hour (n=5). The solutions were filtered, washed, and dried (100oC/24 hours). Copper ions amount were measured by XRF (Canberra Inc., USA). The data were analyzed by one way Anova. Mean values (% weight) of copper ions amount were 0.441 ± 0.029 % (0.05 M group), 0.588 ± 0.027 % (0.10 M group), 0.657 ± 0.014 % (0.15 M group), 0.676 ± 0.037 % (0.20 M group), and 0.737 ± 0.039 % (0.25 M group). The Anova showed that CuCl2 concentration influenced copper ions amount significantly (p<0.01). The differences among the groups were significant, except between 0.15 M and 0.20 M groups (p>0.05). Copper (II) chloride concentration influenced the copper ions sorption on natural zeolite from Wonosari. The 0.25M CuCl2 solution gave the highest copper ions amount.
EFEK KOMPOSISI PARAFIN DAN MALAM LEBAH TERHADAP EKSPANSI TERMAL LINIER MALAM MODEL: EFFECT PARAFFIN AND BEESWAX RATIO TO LINEAR EXPANSION OF MODELLING WAX Fitri Isnaini; Dyah Irnawati; Widowati Siswomihardjo
Dentika: Dental Journal Vol. 14 No. 1 (2009): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.273 KB) | DOI: 10.32734/dentika.v14i1.1966

Abstract

Modelling wax is a pattern wax for complete denture fabrication. It consists of various waxes and other components. Thecomposition influenced the physical properties of modelling wax. The objective of this study was to examine the effect ofparaffn to beeswax ratio on the linear thermal expansion (LTE) of modelling wax. The modelling waxes consist of paraffin (Pertamina, Kalimantan), beeswax (SEA, Yogyakarta), carnauba wax (PT Bratachem, Indonesia), and dammar(Kalimantan). Five compositions of waxes were made with different paraffin to beeswax ratio (paraffin: beeswax: carnauba wax: dammar in % weight): K1 = 70:20:4:6, K2 = 72.5:17,5:4:6, K3 = 75:15:4:6, K4 = 77.5:12.5:4:6, and K5 =80:10:4:6. Six specimens (305 x 22.2 x 14.3 mm) were made of each composition. The specimens were immersed inbathing water at 25°C and 40°C for 20 minutes, and the specimen length was measured by travelling microscope aftereach immersion. The percentage of the LTE was measured. Data were analyzed by one-way Anova and LSD0.05. Theresults showed that the LTE of modelling waxes was between 0.27 + 0.02 % (70:20 ratio) and 0.73 + 0.06 % (80:10ratio). The Paraffin to beeswax ratio influenced the LTE of modelling waxes and showed the significant differencesbetween all compositions (p<0.5). As conclusion, the paraffin to beeswax ratio influenced the linear thermal expansion ofmodelling wax.
EVALUATION ON RESIDUAL MONOMER OF HDDMA MATRIX SYSTEM ON FIDER REINFORCED CONDOSITES (FRC) : EVALUASI MONOMER RESIDU DARI SISTEM MATRIKS HDDMA PADA FIDER REINFORCED CONDOSITES(FRC) Siti Sunarintyas; Widowati Siswomihardjo; Dyah Irnawati; Jukka Pekka Matinlinna
Dentika: Dental Journal Vol. 18 No. 2 (2014): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (722.797 KB) | DOI: 10.32734/dentika.v18i2.2022

Abstract

Matrix system used bis-GMA was reported hazardously. An alternative monomer such as 1,6-hexanediol dimethacrylate(HDDMA) was under research. The aim of this study was to evaluate residual monomer content of HDDMA basedmatrix compositions on FRCs (fiber-reinforced composites). Three monomers based on bis-GMA (Sigma-Aldrich,USA),methylmethacrylate (MMA, ProSciTech, Australia), HDDMA (Esstech, USA) were used and also camphorquinone (CQ,Esstech, USA), N,N-cyanoethyl methylaniline (CEMA, Esstech, USA), E-glass fibers (StickTech, Finland). The matrixratios (weight %) were 78.4% bis-GMA+19.6% MMA+1.0% CQ+1.0% CEMA (control-group), 78.4% HDDMA+19.6% MMA+1.0% CQ+1.0% CEMA (EXP-1group), and 49.0% HDDMA+49.0% MMA+1.0% CQ+1.0% CEMA(EXP-2group). Samples with fibers embedded in matrix were light-cured then powdered. Powder of 150mg was dilutedin acetonitrile to 10ml. The solution was filtered then injected into HPLC (20µL). Residual monomer content wasevaluated by mobile phase of acetonitrile and water of 7:3, flow rate 1mL/minute. The size of column was C185µ,125mm length, 4mm diameter. UV detection used 275nm. Data were analyzed by ANOVA. The result showed theaverage of residual monomer content (in %) was: 0.02125±0.00018 (control-group), 0.01660±0.00016 (EXP-1group),0.01676±0.00033 (EXP-2group). The ANOVA showed significant difference of monomer content among the groups(p<0.05). The LSD showed significant difference between EXP-1 and control-groups; also between EXP-2 and controlgroups (p<0.05). There was no significant difference between EXP-1 and EXP-2 groups (p>0.05). In conclusion, a resinmatrix system based on HDDMA–MMA (EXP-1 and EXP-2 groups) revealed significant difference of residualmonomer content to bis-GMA–MMA (control-group) system. The HDDMA-MMA matrix system had less residualmonomer content than bis-GMA-MMA.
Ekspansi termal linier dan residu malam inlei gigi dengan komponen parafin Indonesia Dyah Irnawati; Widjijono Widjijono; Harsini Harsini
Jurnal Teknosains Vol 10, No 1 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.46548

Abstract

Inlay wax is used for pattern of metallic inlays, crowns, and fixed partial dentures. Inlay wax must have a linear thermal expansion (LTE) and a residue that comply the standards. Inlay wax contains paraffin, carnauba, and beeswax. Paraffin and beeswax are produced in Indonesia. This study aims to examine the effect of the inlay wax compositions with paraffin and beeswax Indonesia on the LTE and residue properties. The research materials are paraffin (Pertamina, Balikpapan), beeswax (SEA, Yogyakarta), carnauba wax (Brataco Chemicals, Yogyakarta), and inlay wax (GC, Japan). Five inlay wax compositions are made with a ratio of paraffin, carnauba, and beeswax 60: 35: 5 (K-60), 65: 30: 5 (K-65), 70: 25: 5 (K-70), 75:20 : 5 (K-75), and 80: 15: 5 (K-80) (% w/w). The waxes were melted at 75 ± 5 oC then moulded for the TLE (267x6.35 x6.35 mm) and the residue (1 g) test specimens (n=4). The LTE and residue specimens were also made of paraffin, carnauba wax, beeswax, and commercial wax (n = 4). The LTE and residue tests were carried out based on ANSI/ADA specification No.4. Data were statistically analyzed by analysis of variance and LSD (α = 0.05). Anava test results showed that composition and temperature had a significant effect on LTE (p <0.05) and composition had a significant effect on residues (p <0.05). The LSD test results showed a significant difference in LTE of the K-75 group with other groups and the residues of the K-75 group and K-80 group with other groups (p <0.05). Inlay wax compositions with high paraffin concentrations have high LTE and residue. The value of LTE and residue of inlay wax with paraffin and beeswax from Indonesia are in accordance with the ANSI/ADA specification no. 4.
THE INFLUENCE OF Cu CONCENTRATION ON Cu-ZEOLITE TO ANTIBACTERIAL POWER IN STREPTOCOCCUS MUTANS Dyah Irnawati; Purwanto Agustiono; Endi Hanifah Wardhani
Jurnal Zeolit Indonesia Vol. 9 No. 2 (2010)
Publisher : Jurnal Zeolit Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Microorganisms on the mouth can cause disease on hard and soft muscular of the mouth. One of the microorganisms is a bacteria S. mutans which become etiology of dental caries. The copper (Cu) is one of chemical agents to control microorganism growth. The copper could be combined with zeolite as antibacterial materials. This study aimed to known the affect Cu concentration on Cu-Natural zeolite to antibacterial power of S. mutans. The study was conducted by making five groups of Cu-Zeolite are 0,05M, 0,1M, 0,15M, 0,2M, and 0,25M. 100 mesh zeolite powder was activated at temperature 200 oC during one hours. CuCl2 solution with concentration of 0,05 M until 0,25 M are made each with volume of 80 mL. Cu-zeolite made by reacting 2 gram of zeolite powder with 80 ml of CuCl2 solution for one hours at temperature of 100 oC, then filtered, washed, and dried on the oven at 100 oC during 24 hours. S. mutans bacterial (0,1 mL x 108 CFU/mL) were grown in solid media MHA, then Cu Zeolite (30 mg) was included on pitting at gel media an incubated for 24 hours at temperature 37 oC (n=5). Transulent zone diameter was measured with digital slide-term (0.01 mm). The data were analyzed by anava one stripe and HSH with level signification of 0,05. The result showed an average of translucent zones was 0 mm (0,05 M gropus), 16,03 + 0,47 mm (0,10M), 16,45 + 0,91 mm (0,15M), 18,08 + 0,39 mm (0,20M), and 18,26 + 0,68 mm (0,25M). Anava test showed Cu concentration on 0,10 M up to 0,25 M of Cu Natural zeolite was significantly effect on translucent zones of S. mutans (p< 0,01). The result of HSD showed a significantly difference among all gropus, except on 0,01 M and O,15 M, and 0,20 m and 0,25 m (p>0,05). Conclusion of this study is Cu concentration in Cu-natural zeolite has affected antibacterial power of S. mutans. 0,01 M of Cuconcentration in Cu-natural zeolite has an antibacterial power to S. mutans and highest antibacterial power was on concentration groups of 0,20M.
Mechanical properties of carving wax with various Ca-bentolite filter composition Widjijono Widjijono; Purwanto Agustiono; Dyah Irnawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 3 (2009): September 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.079 KB) | DOI: 10.20473/j.djmkg.v42.i3.p114-117

Abstract

Background: The carving wax is used as a medium in dental anatomy study. This wax composes of many waxes and sometimes a filler is added. Carving wax is not sold in Indonesian market. Whereas the gradients of carving wax such as beeswax, paraffin and bentonite are abundant in Indonesia. Based on that fact, to make high quality and standard,the exact composition if this carving wax should be known. Purpose: The aim of this study was to investigate the effect of carving wax composition with Ca-bentonite filler on the melting point, hardness, and thermal expansion. Methods: Five carving wax compositions were made with paraffin, Ca-bentonite, carnauba wax, and beeswax in ratio (% weight): 50:20:25:5 (KI), 55:15:25:5 (KII), 60:10:25:5 (KIII), 65:5:25:5 (KIV), 70:0:25:5(KV). All components were melted, then poured into the melting point, hardness, and thermal expansion moulds (n = 5). Three carving wax properties were tested: melting point by melting point apparatus; hardness by penetrometer; thermal expansion by digital sliding caliper. The data were analyzed statistically using One-Way ANOVA and LSD0.05. Result: The Ca-bentonite addition influenced the melting point and thermal expansion of carving wax with significant differences between KI and other groups (p < 0.05). Ca-bentonite addition influenced the carving wax hardness and the mean differences among the groups were significant (p < 0.05). Conclusion: Ca-bentonite filler addition on the composition of carving wax influenced the physical and mechanical properties. The carving wax with high Ca-bentonite concentration had high melting point and hardness, but low thermal expansion.
Color stability of visible light cured composite resin after soft drink immersion Alizatul Khairani Hasan; Siti Sunarintyas; Dyah Irnawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 3 (2009): September 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.059 KB) | DOI: 10.20473/j.djmkg.v42.i3.p123-125

Abstract

Background: Composite resin is a tooth-colored filling material containing Bis-GMA which exhibits water sorption properties. People tend to consume soft drink with various colors. Water sorption properties can alter the color stability of composite resin purpose. Purpose: This study was to determine the influence of immersion durations of composite resin in soft drink on color stability. Methods: The visible-light cured hybrid composite resin and soft drink were used. Ten disk specimens (2.5 mm thickness and 15 mm diameter) of composite resin were prepared and light cured for 20 seconds, then stored in distilled water for 24 hours at 37° C. The initial color of specimens were measured by Chromameter. After that, each specimen was immersed in 30 ml of soft drink up to 48, 72, and 96 hours at 37° C. The specimens’ color were measured again after each immersion. The color changes were calculated by CIE L*a*b* system formula. The data was analyzed by One-Way ANOVA and LSD (α = 0.05). Result: The ANOVA showed that the immersion durations of composite resin in soft drinks had significant influence on the color stability (p < 0.05). The LSD0.05 tests showed significant differences among all groups. The least color change was detected from the group of 48 hours immersion, while the greatest color change was from the group of 96 hours immersion. Conclusions: The immersion of composite resin in soft drinks influenced the color stability (began after 48 hours immersion).
Storage duration effect on deformation recovery of repacked alginates Siti Sunarintyas; Dyah Irnawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 3 (2009): September 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.889 KB) | DOI: 10.20473/j.djmkg.v42.i3.p126-129

Abstract

Background: Manufacturers supply alginate impression materials as a powder that is packaged in bulk and in individual container. Some Indonesian dental suppliers often repackage the bulk alginate into individual plastic packages which are not tied tightly and stored in the display room without air conditioner. It is known that critical factors to the shelf life of alginate includer avoidance of moisture contamination which may lead to premature setting of the alginate and avoidance of high temperature which may cause depolymerization of the alginate. Purpose: The aim of this study was to determine storage duration effect of repacked alginates on deformation recovery. Methods: Two brands of alginates (Tulip®TU, and Aroma Fine DF III®AF) were repacked into 120 plastic containers. The samples were stored in room condition (temperature 29° C ± 1° C, relative humidity 60% ± 10%) for 1, 2, 3, 4 and 5 weeks. The alginates setting time and recovery from deformation were measured according to the ANSI/ADA specification number 18 (ISO 1563). result: The results revealed that there was decreased setting time during 5 weeks but there was slight decreased in deformation recovery after 3 weeks storage. The ANOVA showed there was no significant difference of alginates deformation recovery among the storage times (p > 0.05). Conclusion: Storage duration of repacked alginates in plastic containers during 5 weeks in room condition do not influence the alginate deformation recovery.
Functional relationship of room temperature and setting time of alginate impression material Dyah Irnawati; Siti Sunarintyas
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 3 (2009): September 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.215 KB) | DOI: 10.20473/j.djmkg.v42.i3.p137-140

Abstract

Background: Indonesia is a tropical country with temperature variation. A lot of dental clinics do not use air conditioner. The room temperature influences water temperature for mixing alginate impression materials. Purpose: The aim of this study was to investigate the functional relationship of room temperature and initial setting time of alginate impression materials. Methods: The New Kromopan® alginate (normal and fast sets) were used. The initial setting time were tested at 23 (control), 24, 25, 26, 27, 28, 29, 30 and 31 degrees Celcius room temperatures (n = 5). The initial setting time was tested based on ANSI/ADA Specification no. 18 (ISO 1563). The alginate powder was mixed with distilled water (23/50 ratio), put in the metal ring mould, and the initial setting time was measured by test rod. Data were statistically analyzed by linear regression (α = 0.05). result: The initial setting times were 149.60 ± 0.55 (control) and 96.40 ± 0.89 (31° C) seconds for normal set, and 122.00 ± 1.00 (control) and 69.60 ± 0.55 (31° C) seconds for fast set. The coefficient of determination of room temperature to initial setting time of alginate were R2 = 0.74 (normal set) and R2 = 0.88 (fast set). The regression equation for normal set was Y = 257.6 – 5.5 X (p < 0.01) and fast set was Y = 237.7 – 5.6 X (p < 0.01). Conclusions: The room temperature gave high contribution and became a strength predictor for initial setting time of alginates. The share contribution to the setting time was 0.74% for normal set and 0.88% for fast set alginates.