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Seleksi Berbasis Marka Molekuler pada Padi Generasi F2 Guna Merakit Galur Padi Harapan Tahan Wereng Coklat Nono Carsono; Gigih Ibnu Prayoga; Neni Rostini; Danar Dono
Agrikultura Vol 27, No 1 (2016): April, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.511 KB) | DOI: 10.24198/agrikultura.v27i1.8471

Abstract

ABSTRACTMolecular Marker-based Selection on F2 Progeny for Development of Promising Rice Lines Resistant to Brown PlanthopperBrown planthopper (BPH) is the major insect pest of rice and accounts for significant yield loss. This experiment was aimed to develop BC1F1 and F3 brown planthopper resistant rice lines. Selection on the basis of SSR markers can be done by using two polymorphic SSR markers, i.e., RM586 dan RM8213, which screened from eight SSR markers for BPH resistant. Sixty-three F2 genotypes from IP-1 (derived from IR-64 x PTB-33) population and twenty F2 genotypes from PP-11 (derived from Pandan Wangi x PTB-33) population were selected and will be used for further research by selfed and backcrossed to recipient parents. Chi-squares test for segregation of DNA bands in F2 generation showed that RM8213 fitted with 1:2:1 Mendelian ratio for controlling photosynthetic rates and trichomes length in IP-1 population. This information could be used in programs to develop a durable brown planthopper resistant rice cultivar.Keywords: BPH, F2 population, Moleculer marker, SSRABSTRAKWereng coklat merupakan salah satu hama utama pada tanaman padi yang mampu menurunkan produksi padi secara nyata. Penelitian ini bertujuan untuk memperoleh galur-galur padi F2 yang memiliki marka-marka yang berasosisasi dengan ketahanannya terhadap wereng coklat. Seleksi pada galur padi F2 hasil persilangan telah dilakukan melalui teknik marka molekuler Simple Sequence Repeat (SSR) menggunakan dua marka SSR yang menunjukkan polimorphisme yaitu RM586 dan RM8213 dari delapan marka yang diskrining. Sebanyak 63 genotip dari populasi IP-1 (hasil persilangan IR-64 x PTB-33) dan 20 genotip dari populasi PP-11 (hasil persilangan Pandan Wangi x PTB-33) untuk disilangkan sendiri maupun disilang balik dengan tetua recipient. Selain itu, hasil analisis Chi-Kuadrat untuk segregasi pita DNA menunjukkan bahwa primer RM8213 memiliki rasio 1:2:1 (dominasi tidak sempurna) dalam mengontrol karakter laju fotosintesis dan panjang trikoma terhadap wereng coklat pada populasi IP-1. Informasi yang diperoleh dari penelitian ini nantinya dapat digunakan untuk program perakitan kultivar padi tahan wereng coklat yang durable.Kata Kunci: Marka molekuler, Populasi F2, SSR, Wereng coklat
Analisis Karakter Penting dan Skrining Marka-marka SSR yang Berasosiasi dengan Gen Ketahanan terhadap Wereng Coklat pada Lima Kultivar Padi Gigih Ibnu Prayoga; Danar Dono; Neni Rostini; Nono Carsono
Zuriat Vol 29, No 2 (2018): Zuriat Vol. 29 No. 2 (Desember 2018)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.967 KB) | DOI: 10.24198/zuriat.v29i2.20716

Abstract

 AbstrakHama wereng cokelat telah lama menjadi masalah utama dalam budidaya padi di Indonesia dan beberapa negara Asia lainnya. Merakit tanaman yang memiliki ketahanan terhadap hama ini, dipandang sebagai pendekatan yang lebih efektif dan ramah terhadap lingkungan dan manusia. Penelitian ini bertujuan untuk memperoleh perbedaan pada karakter penting padi antara kaultivar padi tahan dengan kultivar rentan wereng cokelat, selain itu juga untuk mendapatkan marka-marka SSR yang bisa digunakan untuk Marker Assisted Selection (MAS) dan hubungannya dengan karakter penting. Penelitian ini meliputi upaya pengamatan karakter penting dan skrining delapan marka SSR yang diduga berkaitan dengan gen bph (ketahanan terhadap wereng coklat) pada lima kultivar padi tetua yaitu PTB-33 (genotipe tahan), IR-64, Pandan Wangi, Ciherang, dan Sintanur (kultivar lokal). Pengujian karakter penting pada kultivar PTB-33 memiliki laju fotosintesis yang lebih tinggi (toleran), trikoma yang lebih panjang (antixenosis), dan kandungan protein yang lebih rendah (antibiosis) dibandingkan kultivar lainnya yang diuji. Hasil visualisasi pada delapan marka yang memperlihatkan bahwa RM8213 menunjukkan adanya polimorphisme pita DNA pada genotipe tahan PTB-33 dengan kultivar lainnya. Selain itu, marka RM586 dan RM589 juga menunjukkan adanya polimorphisme pada kultivar PTB-33 dengan kultivar lainnya kecuali dengan kultivar IR-64. Hasil analisis Z-Mantel antara marka SSR dengan karakter karakter penting tetua menunjukkan bahwa gen Qbph4 dan Bph17(t) mempunyai korelasi yang sangat tinggi dengan karakter laju fotosintesis dan panjang trikoma, sedangkan gen gen Bph3 dan bph4 berkorelasi tinggi dengan kandungan protein. Kata kunci : gen bph, karakter penting, marka, visualisasi, wereng cokelat. AbstractBrown planthopper (Nilaparvata lugens Stal) is well-known as the major pest in rice cultivation in Indonesia and other countries in Asia. Developing resistant rice lines  is the ideal option for economic and effective management. The experiments were aimed to obtain the important traits between Brown planthopper (BPH) resistant and susceptible genotypes, to obtain SSR markers that can be used in Marker Assisted Selection (MAS) and association of SSR markers with important traits. In this study, characterization of important traits and screening of eight SSR markers were performed for five genotypes i.e. PTB-33 (resistant genotype), IR-64 (tolerant), Pandan Wangi (susceptible), Ciherang (susceptible), and Sintanur (susceptible). PTB-33 showed higher photosynthetic rate (tolerant ability), longer trichomes size (antixenosis ability), and lower protein content (antibiosis ability) compared with other cultivars. RM8213 exhibited visible polymorphic bands between PTB-33 and other cultivars, meanwhile RM586 and RM589 showed polymorphic bands between PTB-33 and other cultivars, except with cv. IR-64. Z-mantel test for correlation between physiological traits and SSR markers showed that Qbph4 and Bph17(t) genes highly correlated with photosynthetic rates and trichomes length, whereas Bph3 and bph4 genes showed a high level of correlation with protein content. Keywords : brown planthopper, important traits, marker, bph gene, visualization
Seleksi kacang tanah (Arachis hypogaea L.) lokal Bangka toleran cekaman salinitas Gigih Ibnu Prayoga; Eries Dyah Mustikarini; Novin Wandra
Jurnal Agro Vol 5, No 2 (2018)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/3366

Abstract

Seleksi cekaman salinitas kacang tanah dilakukan untuk mendapatkan tetua yang toleran terhadap salinitas dan memperbaiki sifat kacang tanah dalam kegiatan pemuliaan tanaman. Informasi genotip unggul kacang tanah toleran terhadap salinitas sangat diperlukan sebagai dasar pemilihan genotip tetua yang adaptif pada lahan salin. Penelitian ini bertujuan untuk memperoleh kacang tanah yang memiliki sifat toleran cekaman salinitas dan menentukan konsentrasi air laut yang dapat ditoleransi oleh tanaman. Penelitian ini dilaksanakan di Kebun Percobaan dan Penelitian, Jurusan Agroteknologi, Fakultas Pertanian Perikanan dan Biologi, Universitas Bangka Belitung, pada bulan Februari–April 2018. Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola split plot dengan 2 ulangan. Petak utama adalah tingkat salinitas yaitu non-salin (kontrol), salinitas rendah, dan salinitas sedang. Anak petak adalah 5 genotip kacang tanah yaitu aksesi lokal (Belimbing dan Arung dalam) dan varietas nasional (Tuban, Kancil, dan Hypoma). Hasil penelitian menunjukkan bahwa varietas Hypoma memiliki karakter jumlah daun dan diameter batang yang paling baik, namun tidak toleran terhadap cekaman salinitas sedang. Aksesi Belimbing merupakan genotip toleran salinitas rendah berdasarkan nilai indeks toleransi cekaman salinitas. Selection of groundnut tolerant to salinity stress is carried out to obtain parent genotypes tolerant to salinity and improve the characteristics of groundnut in plant breeding program. The information of superior groundnut genotypes tolerant to salinity is necessary as the basic of genotypes selection adaptive in the saline area. The research aimed to obtain the groundnut tolerant to salinity stress and determine the concentration of seawater that can be tolerated by groundnut. This research was conducted at The Experiment and Research Field, Faculty of Agriculture Fisheries and Biology, University of Bangka Belitung, from February to April 2018. The research used Complete Randomized Design (CRD) split plot with two replications. Main plot was concentrations of seawater; non-saline (control), low salinity, and moderate salinity. The subplot was groundnut genotypes of local accessions (Belimbing and Arung Dalam) and national varieties (Tuban, Kancil, and Hypoma). The results of this research indicated that Hypoma has the best result for plant height and diameter of stem, but intolerant to moderate salinity stress. Belimbing was the genotype with low salinity tolerance based on score index of tolerant salinity stress.
UPAYA MENINGKATKAN EKONOMI MELALUI BLOK KOMPOS DAN JAMUR TIRAM Yusuf Yusuf; Gigih Ibnu Prayoga; Christianingrum Christianingrum; Anggraeni Yunita
Diseminasi: Jurnal Pengabdian kepada Masyarakat Vol. 4 No. 1A (2022)
Publisher : Pusat Pengabdian kepada Masyarakat- LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/diseminasiabdimas.v4i1A.2994

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Jamur Tiram merupakan peluang usaha yang belum digiatkan dengan baik padahal olahan produk ini diminati masyarakat dan hingga sekarang masih belum dapat memenuhi kebutuhan pasar sehingga harga jualnya menjadi mahal. Rumah Aspirasi Pemuda Bangka Belitung menggagas ide membudidayakan Jamur Tiram dan Blok kompos. Dipilih Jamur Tiram, karena bahan baku tersedia cukup banyak dan memiliki lahan yang baik. Sedangkan blok kompos adalah upaya diversifikasi atas olahan limbah sampah warga yang sudah dilakukan. Kelurahan Pangkalarang memiliki potensi yang baik untuk budidaya jamur tiram. Namun, warga belum mengetahui secara baik tentang jamur tiram, manfaat, maupun cara budidayanya. Unit pengelolaan limbah sampah mengolah hanya menjadi pupuk kompos, sehingga perlu pengenalan bahan pembuatan dan praktik blok kompos. Oleh karena itu, perlu dilakukan kegiatan Pengabdian Kepada Masyarakat (PkM) untuk mengatasinya. Kegiatan dilakukan dalam dua sub kegiatan. Pertama adalah membuat blok kompos dan kedua budidaya jamur tiram. Kedua sub kegiatan tersebut meliputi tahap survei, persiapan, sosialisasi, pelatihan, dan monitoring. Materi sosialisasi yang disampaikan adalah pemanfaatan dan pembuatan blok kompos serta pengenalan jamur tiram, manfaat, syarat tumbuh, dan cara budidaya jamur tiram. Disampaikan juga prospek peluang pasar. Produksi jamur tiram sudah 3 kali panen, yaitu bulan Agustus, September dan Oktober. Manfaat yang diperoleh adalah usaha baru yang dapat meningkatkan perekonomian masyarakat.
Seleksi Aksesi Padi Lokal Bangka Melalui Pengujian Variabilitas dan Heritabilitas Gigih Ibnu Prayoga; Eries Dyah Mustikarini; Desti Pradika
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 1 No 2 (2017): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v1i2.8

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Selection of parent plant is the first step in hybridization activities. The parent plant usually come from germplasms because it has a high diversity and good potential. Testing the potential of germplasm can be done by variability and heritability test. The purpose of this research was to selection of parent plant for plant breeding activity based on the value of variability and heritability. The research was conducted at the Experimental Garden of Faculty of Agriculture, Fisheries, and Biology, Bangka Belitung University. The research used experimental methods by was Randomized Block Design (RBD) with 3 blocks. The treatment are 7 accessions is Grintil, Balok Runti, Mukud Besak, Mayang Curui, Payang Tebing, Balok Lutong and Balok Lukan Jintan. The results showed that there were character differences between 7 local rice accessions of Bangka. The 7 local rice accessions of Bangka have high heritabilities value on The results showed that there were character differences between 7 local rice accessions of Bangka. The 7 local rice accessions of Bangka have high heritabilities values for plant height, flowering time, long panicle, total empty grain, harvest time, long seed, seed width, weight of 1000 seeds, total seeds, and weight seed/plant. Wide variability was found in long seed character. Balok Runti and Payak Tebing were recommended as the parent plants for further breeding activities.
Keragaman Plasma Nutfah Pisang (Musa sp) di Pulau Bangka Berdasarkan Karakter Morfologi Lesta Lesta; Eries Dyah Mustikarini; Gigih Ibnu Prayoga
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 2 No 1 (2018): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v2i1.16

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Banana (Musa sp) is a plant that has good nutrion, mineral, vitamin A, B complex, vitamin C, and vitamin B6. Germplasm diversity of local banana in Bangka has never been identified. Research about germplasm diversity of banana in Bangka Island need to maintain germplasm of Bangka local banana. This research was conducted in Bangka Island. Characterization of banana fruit germplasm includes on qualitative and quantitative traits. Analysis of morphological relationship of banana fruit germplasm used UPGMA. Exploration result obtained 22 germplasms of Bangka local banana. The result of relationship analysis showed four clusters at 0,40 (40%) coefficient. Cluster one consists of Udang germplasm. Cluster two consists of Jernang and Rotan germplasm. Cluster three consists of Wei, Kapal and Abu germplasm. Cluster four consists of Madu Manis, Madu Keling, Gambur, Jambi, 40 Hari, Bawang, Geda, Tematu, Serindit, Masak Ijau, Rejang, Madu Pulau, Kecit Lantai, Susu, Gede and Lilin germplasm. Bangka local banana have wide variability in almost morphological character observed.
Karakterisasi Plasma Nutfah Kacang Tanah (Arachis hypogaea L.) Lokal Asal Bangka Berdasarkan Karakter Morfologi: Karakterisasi Plasma Nutfah Kacang Tanah (Arachis hypogaea L.) Lokal Asal Bangka Berdasarkan Karakter Morfologi Riwan Kusmiadi; Gigih Ibnu Prayoga; Fitra Apendi; Alfiansyah Alfiansyah
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 2 No 2 (2018): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v2i2.25

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Groundnut production can be improved through the use of superior seeds derived from landrace germplasm. The aim of this research was to study the morphological characteristics of Bangka landrace groundnut and the relationship between landrace. This research was conducted in Experimental and Research Garden, Bangka Belitung University from March to August 2017. This research used RBD with 9 landrace accessions and 1 national variety as treatment. The 9 landraces are accession Bedeng Akeh, Lubuk kelik, Matras, Sungailiat, Arung Dalam, Belimbing, Jongkong, Air Ketimbai 1, and Air Ketimbai 2. National variety is Kancil variety. The analysis of genetic relationship was done by the UPGMA method. The result of morphological characterization shows many similarities in the character such as growth habit, number of branches, stem color, leaf color, standard petal color, pod beak, and seed color. The differences are leaflet shape, pod constriction, pod reticulation, and primary seed color. The result of relationship analysis based on morphological characterization showed 4 groups on 87% similarities. The first group consists of the accession of Belimbing, Jongkong, Air Ketimbai 2, Sungailiat, and Kancil varieties. The second group is accession Matras, Lubuk Kelik, and Bedeng Akeh. The third group is Air Ketimbai 1 and the fourth group is Arung Dalam accession.
Seleksi Galur Generasi F4 Padi Beras Merah Tahan Rebah: Seleksi Galur Generasi F4 Padi Beras Merah Tahan Rebah Eries Dyah Mustikarini; Gigih Ibnu Prayoga; Bama Aprilian
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (689.949 KB) | DOI: 10.33019/agrosainstek.v4i1.105

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Lodging of the paddy plants causes a decrease in rice plant production. Lodging resistants rice plants can be produced through plant breeding. The purpose of the research is selection lodging resistant F4 lines. The research was carried out from December 2018 to May 2019 in the ultisol field, Bangka Regency, Bangka Belitung Province. The F4 lines were planted by a single plant design. Plants were selected using the pedigree method. The result showed that the characteristics of rice F4 lines from crossed were less than 90 cm of height and 70 selected F4 lines seemed to have lodging resistance based on the plant height character and also supported by stem diameter, panicle height, stem strength, and pithy seeds weight per clump.
UJI ADAPTASI BERBAGAI GENOTIPE KACANG TANAH DI KOMPOSISI MEDIA TANAM TAILING Afrizal Muchtadin; Eries Dyah Mustikarini; Gigih Ibnu Prayoga
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) Jurnal Bioindustri: VOL. 3 NO. 1 (2020)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v3i1.841

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Groundnut are protein-producing plants that humans need. ground nut production in Bangka Belitung is still low. Efforts to increase groundnut production can be done by utilizing post-tin mining land. The research was conducted at the research experiment gardens University of Bangka Belitung. The purpose of this research is to find the groundnut genotype that is able to adapt to the tailings media. The research design used was Factorial Randomized Block Design (FRBD) consisting of 2 factors. Factor 1 are the genotypes of Jongkong, Belimbing, Air Ketimbai 1, Air Ketimbai 2, Hypoma 1 and Talam 2. Factor 2, which is the composition of 100% ultisol planting media, 100% tailings, 70% tailings + 30% ultisol and 50% + tailings ultisol 50%. The results of this study indicate that groundnut genotypes that have a high level of adaptation are Jongkong on the character of plant height, number of leaves, root volume, number of pods per plant, and number of filled pods per plant. National varieties that have a high level of adaptation are Talam 2 and Hypoma 1 on the character of plant height, canopy wet weight, weighted weight, root volume and number of filled pods per plant. Jongkong accession, Hypoma 1 and Talam 2 varieties can be recommended as groundnut plants for cultivation on tailings land.
Agronomic Character Variability Among Upland Rice Genotypes (Oryza sativa L.) Ricoh Darisman Sihombing; Lailatul Badriyah; Dewi Nawank Sary; Thoriq Ahmad Syauqy; Eries Dyah Mustikarini; Gigih Ibnu Prayoga; Ratna Santi; Budi Waluyo
Nusantara Science and Technology Proceedings Multi-Conference Proceeding Series C
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2610

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Rice production still has to be increased to meet the domestic needs of consumer preferences. Therefore, it is necessary to develop rice genotypes that have agronomic characteristics which can encourage high production. The objective of this research is to examine the variability of agronomic performance as well as the genotypic and phenotypic variability of agronomic traits among rice lines and cultivars. Materials included six promising lines and four cultivars. Urea, SP-36, and KCl fertilizers are used, as well as equipment for sacks, envelopes, fungicides, and pesticides. The research was based on an experiment with a randomized block design. This research was conducted at the Experimental Field of Agriculture Faculty, Universitas Brawijaya in Jatimulyo, Malang, from March to July 2022. According to the data, there is variation in the agronomic performance of the examined genotypes. Based on the coefficient of genotypic variation value, none are classified as high. All characters had a high category of variation based on phenotypic variance.