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Journal : Al-Kimia

Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksan dari Umbi Lobak (Raphanus Sativus Lamk) Ummi Zahra; Muharram Muharram; Asriani Ilyas Asriani Ilyas
Al-Kimia Vol 1 No 1 (2013): June
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (545.813 KB) | DOI: 10.24252/al-kimia.v1i1.1570

Abstract

Isolation and identification of secondary metabolite compound in extract of n-Hexane in Umbi Lobak (Raphanus sativus Lamk) have been carried out. The purpose of this research is to know secondary metabolite compound which is contained in Umbi Lobak (Raphanus sativus Lamk) which obtained from Gowa. The compound was obtained by isolation process that consists of several steps including extraction, fractination, purification, and identification. Extraction was carried out using maceration with n- hexane. The identification has done by phytochemistry test, melting point, solubility, TLC, and IR spectroscopy. The result of research found out that the obtained compound was the steroid compound, with melt point 133-133,5 oC and had positive  reaction toward Lieberman Burchad reagents, where the Lieberman Burchad reagent resulting is green which perfectly solved in n-hexane, bit solved in methanol, and could not be solved in aceton. TLC analysis showed a stain in three scales different solvent and the result of elucidation structure in IR spectrophotometer showed that compound which get is β sitosterol.
Uji Antiseptik Ekstrak Kulit Jeruk Nipis (Citrus aurantifolia) terhadap Bakteri Bacillus cereus dan Escherichia coli Mirnawati Mirnawati; Nur Mu'min; Ummi Zahra
Al-Kimia Vol 9 No 2 (2021): DESEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v9i2.23261

Abstract

Indonesia is one of the countries with the fourth largest natural wealth in the world. The magnitude of the natural potential provides a great opportunity for scientists to develop it as a useful product for society. One of the medicinal plants that can be used is lime (C. aurantifolia). The purpose of this study was to determine the inhibition of lime peel extract (Citrus aurantifolia) against Bacillus cereus, Escherichia coli and mold bacteria using the coloni counter method. This research includes the extraction process of the maceration method then the extract obtained is tested for bacterial analysis using a colony counter. Based on the results of the research conducted, it can be concluded that lime peel extract (C. aurantifolia) has the ability to inhibit the growth of B. cereus and E.coli bacteria, respectively 0 APM/g and <3.0 APM/g. This is because there are bioactive compounds such as -pinene and D-limonene in lime peel extract (C. aurantifolia).