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Parasite Prevalence Oodinium sp. in Cantang Hybrid Grouper Cultivated in Recirculating Aquaculture System Kadek Leni Widiartini; Kadek Lila Antara; Ketut Mahardika; Gede Iwan Setiabudi
Advances in Tropical Biodiversity and Environmental Sciences Vol 6 No 3 (2022): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2022.v06.i03.p03

The aims of this study were to determine clinical symptoms, mortality, prevalence, and histopathology of Cantang hybrid grouper fish infected with Oodinium sp. on the Cantang. The study was conducted by direct observation of clinical symptoms, calculating the number of fish mortality, and measuring the quality of water in the tank using the Cantang. While histopathology was conducted in the laboratory using 10 samples of sick fish with an average total length of 10.6 ± 0.69 cm and a weight of 18.6 ± 1.77 g. The results showed that the fish seen swimming weakly on the surface of the water near aeration or standing still at the bottom of the tub, decreased appetite, had pale or darker body color, and were thin. Observation of skin and gill mucus under a microscope showed the presence of ectoparasites Oodinium sp. in massive quantities in almost every gill sheet. The mortality of fish infected with Oodinium sp. in the Cantang of 26.84±3.9%, with a prevalence of 49.59%. Histopathologically the gill lamellae of fish infected with Oodinium sp. shows the occurrence of hyperplasia which causes the union of several gill lamellae.

The Analysis of Clinical sign, Mortality and Histopathology of Cantang Grouper Infected with VNN in Recirculating Aquaculture System Ni Putu Nadya Susanti; Kadek Lila Antara; Ketut Mahardika; Gede Iwan Setiabudi
Advances in Tropical Biodiversity and Environmental Sciences Vol 6 No 3 (2022): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2022.v06.i03.p01

Hybrid grouper is a variety of grouper that has become increasingly popular in recent years. The development of grouper cultured in Indonesia utilizing the Recirculating Aquaculture System (RAS) is currently underway; nevertheless, fish cultured in the RAS system continue to struggle with infectious diseases, one of which is VNN (viral nervous necrosis) infection caused by piscine betanodavirus. The purpose of this study was to know the clinical sign, mortality, and histopathology of cantang grouper (Epinephelus fuscoguttatus x E. lanceolatus) infected with VNN in RAS. Fish samples were obtained from the hatchery using RAS. This research uses a mixed methods approach and the type of research used is exploratory research. The parameters observed were clinical signs, mortality, and histopathology. Water quality, including temperature, DO, salinity, and pH at the time of VNN infection, was used as secondary data. The results showed that clinical signs of sick fish were weakness, staying at the bottom of the rearing tank, decreased appetite, swimming with the stomach facing up and the body dark in color. Fish mortality occurred for ± 10 days with a peak of mortality reaching 26.86 ± 7.45% on day 9 from initial signs. Histopathology result, diseased fish showed vacuolization in the brain and retina of the eye.

Analysis Of Water Quality Conditions In Pearl Oyster (Pinctada maxima) Hatcheries in Karangasem Bali Dewi Wulandari; Made Dwipa Kusuma Maharani; Gede Iwan Setiabudi
Advances in Tropical Biodiversity and Environmental Sciences Vol 7 No 1 (2023): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2023.v07.i01.p05

Indonesia has a high potential for pearl oyster farming due to its diverse marine habitats, warm waters, and abundant supply of seaweed, which is the preferred food for pearl oysters. Indonesia is home to several species of pearl oysters, including the Pinctada maxima, which are prized for their large size and the quality of their pearls. Water quality plays a crucial role in the cultivation of Pinctada maxima. Factors such as temperature, pH, salinity, dissolved oxygen levels, and the presence of pollutants and pathogens can all impact the health and survival of Pinctada maxima. In addition to feed quality and seed availability, good water quality management is the key to the success of this biota seedling. This research was carried out by measuring parameters including temperature, salinity, pH, and dissolved oxygen levels in the waters temporally in the morning and evening every day of cultivation. Variables of ammonia, nitrite, total suspended solids, total bacteria, and vibrio bacteria were carried out once a week. The results of temperature measurements range from 28.2-290C. Measurement of total suspended solids 0 mg/l. pH measurements ranged from 8.1-8.3. Dissolved oxygen measurements ranged from 4.16-4.27 mg/l. Salinity measurements obtained results of 35 ppt. Ammonia measurements range from 0-1.55 mg/l. Nitrite measurements range from 0.012-0.026. Measurement of total bacteria obtained <1 X 101 cfu/ml from all samples. Measurement of total vibrio yielded <1 X 101 cfu/ml from all samples. Based on the research that has been done, the results show that water quality is still in the optimal range for pearl oyster (Pinctada maxima) hatchery activities.

Potensi Muck Dive di Perairan Kecamatan Buleleng Bali I Nyoman Dodik Prasetia; Gede Iwan Setiabudi; Kadek Lila Antara; Jasmine Masyitha Amelia; Gressty Sari Br Sitepu; Made Dwipa Kusuma Maharani; Dewi Wulandari; I Nyoman Suardana
Buletin Oseanografi Marina Vol 12, No 2 (2023): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v12i2.50061

Kawasan PesisirÂ Kecamatan Buleleng terdiri dari Pantai Desa Kalibukbuk, Anturan, Tukad Mungga, Pemaron, Baktiseraga, Banyuasri, Kaliuntu, Kampung Anyar, Kampung Bugis, Kampung Baru, Banyuning, dan Penarukan merupakan kawasan perairan yang berada di pusat Kabupaten Buleleng.Â Perairan Kecamatan Buleleng memiliki karakteristik subsrat perairan berupa hamparan terumbu karang, patahan karang, pasir yang didominasi oleh pasir hitam, dan berlumpur.Â Kondisi subsrat memiliki potensi pengembangan wisata muck dive sebagai alternatif wisata bahari yang berkelanjutan.Â Muck dive merupakan jenis penyelaman yang dilakukan di subsrat berlumpur dengan berfokus untuk menemukan jenis langka dan samar yang jarang ditemukan di terumbu karang.Â Penelitian bertujuan mengetahui potensi muck dive dengan memperkuat data identifikasi jenis, kelimpahan dan keanekaragaman organisme yang dapat dijadikan sebagai ikon obyek wisata selam.Â Peneltian menggunakan metode underwater visual sensus dengan mengikuti line transect. Penelitian menunjukkan Perairan Kecamatan Buleleng memiliki potensi pengembangan wisata muck dive dengan karakteristik di setiap perairan desa yang beragam berdasarkan tipe subsrat perairan.Â Kelimpahan dan keanekaragaman jenis organisme sebagai obyek muck dive tertinggi terdapat di Pantai Desa Baktiseraga, Banyuasri, dan Kaliuntu, sedangkan terendah di Desa Kampung Anyar, Kampung Bugis, Kampung Baru, Banyuning, dan Penarukan. Potensi wisata muck dive layak dikembangkan sebagai alternatifÂ wisata bahari yang dikelola secara berkelanjutan.Â Â Â The coastal area of Buleleng districts consists of Kalibukbuk, Anturan, Tukad Mungga, Pemaron, Baktiseraga, Banyuasri, Kaliuntu, Kampung Anyar, Kampung Bugis, Kampung Baru, Banyuning, and Penarukan are water areas located in the center of Buleleng Regency. The Buleleng districts water have the characteristics of the water substrate in the form of coral reefs, coral fractures, sand dominated by black sand, and muddy. Substrate conditions have the potential to increase muck dive tourism as an alternative to sustainable marine tourism. Muck dive is a type of diving that is carried out in muddy substrates with a focus on finding rare and rare species that are rarely found in coral reefs. This study aims to determine the potential of muck dive by strengthening the identification data of species, abundance and diversity of organisms that can be used as icons of diving tourism objects. The research uses the underwater visual census method by following the line transect.The research shows that the Buleleng districts waters have the potential to develop muck dive tourism with different characteristics in each village waters based on the type of substrate. The highest abundance and diversity of organisms as muck dive objects was found in Baktiseraga, Banyuasri, and Kaliuntu Beaches, while the lowest was in Kampung Anyar, Bugis, Kampung Baru, Banyuning, and Penarukan villages. This potential to be the one of alternative for marine tourism which is must managed sustainably.

PENGARUH PERBEDAAN SUHU TERHADAP LAJU PERTUMBUHAN DAN KELULUSAN HIDUP BENIH IKAN KOI (Cyprinus carpio) Susi Puspita Sari; Jasmine Masyitha Amelia; Gede Iwan Setiabudi
Jurnal Perikanan Unram Vol 12 No 3 (2022): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v12i3.328

Ikan hias yang terkenal dikalangan masyarakat umum salah satunya ialah ikan hias jenis Koi. Namun dalam proses budidaya ataupun pembesaran salah satu hal yang sering menjadi perhatian pada saat perkembangan ikan Koi adalah suhu yang dapat mempengaruhi tingkat pertumbuhan dan daya tahan tubuh serta timbulnya berbagai penyakit. Analisis ini dilakukan dengan tujuan untuk mengetahui seberapa besarnya pengaruh suhu terhadap laju perkembangan dan daya tahan hidup ikan Koi. Metode rencana eksplorasi yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok (RAK) dengan 3 perlakuan, dimana setiap perlakuan tersebut diulang sebanyak 3 kali. Dengan perlakuan yang digunakan adalah P¹ (28?C), P² (30?C) dan P³ (32?C). Hasil penelitian menunjukkan bahwa laju perkembangan yang paling menonjol terdapat pada perlakuan P¹ dengan pertumbuhan panjang 2,9 cm dan pertambahan berat 8 g, kemudian disusul oleh P² dengan pertumbuhan panjang 1,8 cm dan pertambahan berat 5,6 g. dan perkembangan paling sedikit terdapat pada perlakuan P³ dengan pertumbuhan panjang 1,3 cm dan pertambahan berat 3,1 g. Nilai daya tahan hidup ikan Koi selama pemeliharaan di setiap perlakuan rata-rata 100%. Parameter pendukung kualitas air yang didapat adalah pH pada kisaran 6,9-8,5 dan DO berkisaran 5,27-7,76 ml/L. Perbedaan suhu secara signifikan mempengaruhi laju perkembangan dan tidak signifikan terhadap daya tahan hidup ikan Koi.

UJI EFEKTIVITAS EKSTRAK BIJI PALA (Myristica fragrans Hout) SEBAGAI ANTIBAKTERI TERHADAP PERTUMBUHAN Vibrio parahaemolyticus PENYEBAB PENYAKIT VIBRIOSIS PADA IKAN KERAPU (Epinephelus spp.) IN VITRO Rizky Aldi Zulfaizi Khairiyah; Gede Iwan Setiabudi; Indah Mastuti; Ketut Mahardika
Jurnal Perikanan Unram Vol 12 No 3 (2022): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v12i3.335

Salah satu ikan yang paling unggul untuk dibudidayakan di Indonesia ialah kerapu. Akan tetapi komoditas ini sering dihadapkan oleh kematian masal yang di akibatkan oleh penyakit vibriosis seperti bakteri V. parahaemolyticus. Telah banyak pemakaian antibakterial yang berasal dari bahan alami yang terus dilakukan pengembangan. Biji pala (Myristica fragrans Hout) ialah tanaman yang masih tradisional dengan kandungan zat antibakterialnya yang banyak. Dilaksanakannya pengujian ini yakni guna mengetahui efektivitas dari ekstrak biji pala (Myristica fragrans Hout) sebagai antibakteri terhadap pertumbuhan V. parahaemolyticus secara in vitro. Metode penelitian menggunakan metode eksperimen dengan rangcangan acak lengkap non faktorial. Biji pala dilakukan proses ekstraksi dengan pemakaian etanol 96% dan pemanasan rendah dengan metode maserasi. Uji efektivitas ekstrak biji pala pada konsentrasi 5%, 10%, 20% dan 40% terhadap V. parahaemolyticus dengan metode yang digunakan yakni cakram disk. Erythromycin sebagai kontrol positif dan aquades sebagai kontrol negatif. Hasil penelitian menunjukan bahwasanya ekstrak biji pala konsentrasi 40% memiliki zona hambat terhadap V. parahaemolyticus paling tinggi yaitu, 10,8±0,57 mm (P<0,05) dibandingkan dengan ekstrak biji pala pada konsentrasi 5-20% (7,3±0,84, 9,0±0,50, 9,8±0,76 mm). Zona hambat ekstrak biji pala tersebut masih lebih rendah (P<0,05) dibandingkan dengan zona hambat dari erythromycin yaitu 19,5±0,5 mm. Namun demikian, ekstrak biji pala memiliki potensi menghambat pertumbuhan V. parahaemolyticus.

Pengaruh Ekstrak Kulit Bawang Merah Terhadap Daya Hambat Bakteri Aeromonas hidrophilla Penyebab Penyakit Pada Ikan Secara In Vitro Pranata, Andi Welly Wiliandhana; Swasta, Ida Bagus Jelantik; Setiabudi, Gede Iwan
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5739

Aeromonas hydrophila, commonly found in aquatic environments, is a Gram-negative bacterium frequently pathogenic to fish, causing diseases such as hemorrhagic septicemia or Motile Aeromonas Septicemia (MAS) in various freshwater fish species. This study investigated the antibacterial potential of red onion skin extract (Allium cepa L.) extracted using 99% absolute alcohol against the growth of Aeromonas hydrophila. The experiments were conducted with varying extract concentrations of 5%, 10%, 15%, 20%, and 25%, using tetracycline as a control. The results demonstrated that the red onion skin extract was able to inhibit the growth of Aeromonas hydrophila, as indicated by the formation of inhibition zones around the growth media. The average diameters of the inhibition zones produced by the extract at concentrations of 5%, 10%, 15%, 20%, and 25% were 1.21 mm, 2.43 mm, 3.36 mm, 5.70 mm, and 6.23 mm, respectively. Statistical analysis using a One-way ANOVA test with SPSS software showed a p-value of 0.000, indicating a significant difference among the red onion skin extract concentrations. Therefore, a Least Significant Difference (LSD) post-hoc test was performed to identify specific differences between the concentration treatments

Pemanfaatan Citra Satelit Resolusi Tinggi pada Google Earth Pro dan SIG untuk Deteksi Kerusakan Infrastruktur Jalan dan Bangunan Akibat dari Abrasi di Pantai Pebuahan, Desa Banyubiru Bagus Arya Yoga Bharata, Ida; Atmaja, Dewa Made; Gunamantha, I Made; Christiawan, Putu Indra; Setiabudi, Gede Iwan
Jurnal ENMAP (Environment and Mapping) Vol. 6 No. 01 (2025): Maret 2025, Jurnal ENMAP
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar

The coastal environment in some parts of Indonesia's coast is vulnerable to coastal dynamics, one of which is abrasion which threatens the organization at Pebuahan Beach, Banyubiru Village, Negara, Jembrana, Bali Island. The objectives of this research are 1) detecting environmental damage to road infrastructure and buildings resulting from abrasion at Pebuahan Beach, and 2) mapping environmental damage to road infrastructure and buildings resulting from abrasion at Pebuahan Beach. The research carried out was exploratory descriptive research using CSRT data available on Google Earth Pro at 3 different times, namely 2009, 2016, and 2023, which were collected through visual interpretation and digitization on the screen and then analyzed using a spatial approach. The research results showed that 184 buildings were washed away and 28 buildings were damaged during the 2009-2023 period. The percentage of buildings that were washed away and damaged was a total of 30% of buildings affected by abrasion during the 2009-2023 period. Apart from that, damage to road infrastructure covering 675.57 meters was washed away and 268.31 meters was damaged, or if combined, it reached 25% of the existing roads in the Pebuahan Beach residential area during the 2009-2023 period. The continuing damage that occurs makes it necessary to take concrete steps to address the large number of buildings and infrastructure that have been lost.

Pengaruh Ekstrak Kulit Bawang Merah Terhadap Daya Hambat Bakteri Aeromonas hidrophilla Penyebab Penyakit Pada Ikan Secara In Vitro Pranata, Andi Welly Wiliandhana; Swasta, Ida Bagus Jelantik; Setiabudi, Gede Iwan
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5739

Aeromonas hydrophila, commonly found in aquatic environments, is a Gram-negative bacterium frequently pathogenic to fish, causing diseases such as hemorrhagic septicemia or Motile Aeromonas Septicemia (MAS) in various freshwater fish species. This study investigated the antibacterial potential of red onion skin extract (Allium cepa L.) extracted using 99% absolute alcohol against the growth of Aeromonas hydrophila. The experiments were conducted with varying extract concentrations of 5%, 10%, 15%, 20%, and 25%, using tetracycline as a control. The results demonstrated that the red onion skin extract was able to inhibit the growth of Aeromonas hydrophila, as indicated by the formation of inhibition zones around the growth media. The average diameters of the inhibition zones produced by the extract at concentrations of 5%, 10%, 15%, 20%, and 25% were 1.21 mm, 2.43 mm, 3.36 mm, 5.70 mm, and 6.23 mm, respectively. Statistical analysis using a One-way ANOVA test with SPSS software showed a p-value of 0.000, indicating a significant difference among the red onion skin extract concentrations. Therefore, a Least Significant Difference (LSD) post-hoc test was performed to identify specific differences between the concentration treatments

â€œULVAâ€ PENGEMBANGAN TEKNIK PENANDA ALAMI PADA BUDIDAYA ABALON (Haliotis squamata) Permana, Gusti Ngurah; Rusdi, Ibnu; Wibawa, Gigih Satria; Setiabudi, Gede Iwan
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 1 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.7

"ULVA" NATURAL MARKING TECHNIQUES DEVELOPMENT IN ABALONE CULTURE (Haliotis squamata)Â ByÂ Gusti Ngurah Permana,, Ibnu Rusdia, Â Gigih Satria Wibawaa and Iwan SetyabudibTagging is used to obtain some information during the process of selecting and managing population of abalone shells, Haliotis squamata. This research was conducted to determine the natural marker method of abalone, (H. squamata). The samples were used of Â total length of the shell (initial average: 0.8 Â± 0.2 mm).Â Seed tagging using the Ulva sp. performed by giving a sequence of feed at the age of: 2-3 months (Ulva sp.), 3-4 months (Gracilaria sp.), 5-6 months (Ulva sp.), 6-10 months (Gracillaria sp.) and control 2 months (Ulva sp.), 3-10 months (Gracilaria sp.). The results of this study indicated that the tagging of the seeds with the Ulva method has a same growth pattern in bothÂ treatment and control (38.93 Â± 1.5 mm; 39.47 Â± 1.44 mm). The effectiveness of Ulva tagging is seen in toca color finder (TC. 4103 green) and (TC 8135 brown), more sharpen in treatment compared to control. Ulva tagging technology can be applied to abalone seeds to see the sequence of the green color on the shell.Â Â

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