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All Journal HAYATI Journal of Biosciences VALENSI Microbiology Indonesia Acta Biochimica Indonesiana Sarwahita : Jurnal Pengabdian Kepada Masyarakat Jurnal Riset Sains dan Kimia Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia
Jefferson Lynford Declan
(1) Department Of Chemistry, Faculty Of Mathematics And Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim Asj'ari, 6th Floor, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia (2) Research Center For Detection Of Pathogenic Bacteria,
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Efficacy Test of Prototype Kit for Detection Bacillus cereus and Listeria monocytogenes in Processed Meat using Real-time PCR Method Nurjayadi, Muktiningsih; Fahriza, Tiara; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Berkahingrum, Ayu; Anggraeni, Rosita Gio; Putri, Gladys Indira; Declan, Jefferson Lynford; Akbar, Dandy; Maulana, Irvan; Azzahra, Maharanianska; Shangkara, Muhammad Arkent; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abumoelak, Bassam; El Enshasy, Hesham
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.44280

Abstract

According to the World Health Organization (WHO), harmful agents such as Bacillus cereus and Listeria monocytogenes are responsible for 600 million cases of disease and 420,000 deaths annually. This research aims to test the effectiveness of the real-time PCR method for developing a prototype kit to detect pathogenic bacteria in processed meat. As a comparison, the and conventional PCR methods were used to obtain the accuracy, specificity, sensitivity, and effectiveness of the real-time PCR method. All the samples were cultured in solid media agar, performed amplification using specific primers cyt-K 2 and hly using PCR and real-time PCR. Meatballs, nuggets, and sausages, five samples each, were found to be positive positively contaminated with all targeted bacteria. However, they did not provide specific results using solid media culture and the PCR method. In addition, the real-time PCR method using prototype kit formulas accomplished that all contaminated samples had a Ct value smaller than the negative control, NTC (No Template Control), and had a similar melting curve to the positive control. This establishes that the real-time PCR method clarifies that all samples were contaminated with target bacteria. A formula was developed in the prototype kit with real-time PCR methods that can be used and applied on a commercial scale efficiently and precisely.
Co-Authors Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Adinda Myra Amalia Putri Adinda Myra Amalia Putri AGUS SETIAWAN Agus Setiawan Akbar, Dandy Ananda Indah Putri Sihombing Angelina, Helzi Anggraeni, Rosita Gio Anisa Fitriyanti Annisa Maharani Athiyah Layla Atikah Nur Rahmawati Ayu Berkahingrum Azizah, Puan Aqila Azzahra, Maharanianska Bassam Abomoelak Bassam Abomoelak Berkahingrum, Ayu Chen, Shyi-Tien Dalia Sukmawati Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dandy Akbar Juliansyah Dwi Ana Oktaviani El Enshasy, Hesham El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Enshasy, Hesham Ali El Fahriza, Tiara Fera Kurniadewi Fera Kurniadewi Fitriyanti, Anisa Gladys Indira Putri Gladys Indira Putri Gladys Indira Putri Grace Grace, Grace Gusti Angieta Putri Hairishah, Nazrisya Helzi Angelina Hesham A. El Enshasy Hesham Ali Al-Enshashy Hesham Ali El-Enshasy I Made Wiranatha I Made Wiranatha Irma Ratna Kartika Irvan Maulana Irvan Maulana Irwan Saputra Ismaya Krisdawati Ismaya Krisdawati Ismaya Krisdawati Juliansyah, Dandy Akbar Kartika, Irma Ratna Krisdawati, Ismaya Kurniadewi, Fera Maharani Azka Azzahra Maharanianska Azzahra Maulana, Irvan Mellyna Fitriani Muktiningsih Nurjayadi Musie, Royna Rahma Nabilla Anisa Putrie Nasywa Fhelia Salta Nida Nur Afifah NOVITASARI Pardiana Puan Aqila Azizah Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Rahmawati, Atikah Nur Ririn Gustini Rizkahana Syehfia Rosita Gio Anggraeni Royna Rahma Musie Saamia, Vira Saamia4, Vira Saputro, Dwi Anna Oktaviani Sarah Adilisa Kartini Shangkara, Muhammad Arkent Shyi-Tien Chen Sihombing, Ananda Indah Putri Sintia Mardita SITI FATIMAH Siti Fatimah Sri Rahayu SRI RAHAYU Tiara Fahriza Uswatul Nisa Vira Saamia Vira Saamia Vira Saamia Wiranatha, I Made