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Journal : Rumphius Pattimura Biological Journal

DNA Barcoding Application Using CYTB (Cytochrome-B) Marks for Authentication of Patin Fish (Pangasius sp.) Products in Surabaya Area Aulia, Wina Intan Nur; Setiawan, Edwin; Triwibowo, Radestya; Saputro, Triono Bagus; Hidayati, Dewi; Nurhayati, Awik Puji Dyah; Ridho, Muhammad Ainur Rosyid
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p079-092

Abstract

DNA barcoding is a practical and accurate molecular technique for identifying processed fish products that lack morphological characteristics. However, its application is limited by the availability of reference databases, DNA quality, and primer suitability. This study aims to determine the genetic identity of pangasius catfish (Pangasius sp.) marketed in Surabaya using the Cytochrome b (CytB) gene and to evaluate PCR-RFLP as an alternative to sequencing. A total of twenty samples (fresh and frozen fillets) were analyzed through DNA extraction and PCR amplification of the CytB gene, followed by in silico and in vitro analyses.The results show that CytB demonstrates high amplification success and provides sufficient resolution for species-level identification based on Neighbor-Joining analysis with the Kimura 2-parameter model. The study confirms that the samples were genetically identified as Pangasius pangasius and Pangasius hypophthalmus. This research highlights the novelty of integrating in silico prediction with laboratory validation to improve marker evaluation in DNA barcoding studies. Furthermore, it demonstrates that CytB-based PCR-RFLP is a rapid and cost-effective alternative to sequencing for detecting species mislabeling. Overall, the CytB gene is proven to be a reliable molecular marker for species authentication, supporting traceability systems and strengthening seafood authenticity monitoring in commercial markets.
Co-Authors Abdul Wahab Abdul Wahab Adi Setyo Purnomo Arief, M. Romli Arif Luqman Aulia, Wina Intan Nur Aunurohim Aunurohim Awik Puji Dyah Nurhayati Aziz Purwantoro Belva Ariya Mahendra Chokwiwatkul, Ratchata Dewi Hidayati Dianita Indahsari Dini Ermavitalini Dwi Yanuar Rakhma Sari Edwin Setiawan Eka Afiyanti Rohmah Elena Rosalia Firsta Endang Semiarti Endry Nugroho Prasetyo Fadilah, Aulia Sunan Faradiba Lazuardi Rohman Farid Kamal Muzaki Fathin Finariyah Fathin Finariyah, Fathin Hendro Nurhadi Hery Purnobasuki Indah Trisnawati Dwi Tjahjaningrum Indahsari, Dianita Irine Prabhandaru Iska Desmawati Khoirun Nisak Kristanti Indah Purwani Leonardo Pascalis Dimas Cahyo Baskoro Mahendra, Belva Aria MARIA BINTANG Mawardani, Alfiyyana Nurrahma Miftahul Huda Muhibuddin, Anton Ni'matuzahroh Niki Yuliansari Niki Yuliansari, Niki Ni’matuzahroh Ni’matuzahroh Noor Nailis Saadah Noor Nailis Sa’adah Nova Maulidina Ashuri Nur Fadlilatus Sholihah Nur Fadlilatus Sholihah Nur Fadlilatus Sholihah, Nur Fadlilatus Nurul Jadid Oktavianto, Lucky Pinka Langlangdewi Nurrachmamila Rahmahana, Ratna Syifa'a Ridho, Muhammad Ainur Rosyid Rizam, Bilqis Naura Safira Rossy Angelina Latuharhary Sa'adah, Noor Nailis Sa’adah, Noor Nailis Saptarini, Dian Setyo Nugroho Shovitri, Maya Siska Yulia Rukhmana Siti Dianawati Siti Dianawati, Siti Solihah, Anisatus Sri Nurhatika Sumarni Dwirejeki Teguh Herlambang Thalita Meidina Triwibowo, Radestya Vita Siti Fatimah Yohanes Ardian Kapri Negara Yulianto Ade Prasetya Yusmani Prayogo