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All Journal Journal of Food and Pharmaceutical Science Pharmascience Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Window of Health : Jurnal Kesehatan Jurnal Mandala Pharmacon Indonesia Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Hydrogen: Jurnal Kependidikan Kimia Mejuajua Pharmaceutical Reports Journal Microbiology Science Journal of Food and Pharmaceutical Sciences Indonesian Journal of Jamu MPSJ
Selpida Handayani, Selpida
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Uji Aktivitas Antioksidan Ekstrak Daun Karet Kebo (Ficus Elastica) dengan Metode Peredaman Radikal Bebas Dpph (1,1-Diphenyl-2-Picrylhydrazil): Antioxidant Assay of Ficus Elastica Extract Leaf with Dpph Free Radical Scavenging (1,1-diphenyl-2-phycrilhydrazyl) Handayani, Selpida; Kurniawati, Ida; Abdul Rasyid, Faradiba
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 6 No. 1 (2020): (March 2020)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.439 KB) | DOI: 10.22487/j24428744.2020.v6.i1.15022

Abstract

Ficus elastica leaf is one of the Ficus species assumed to be rich in polyphenols so that it can act as an antioxidant. The research aimed to determine the antioxidant activity and IC50 value of methanol and water extracts of rubber leaf. In this research, the extraction was conducted by maceration using methanol and obtained the yield value of 7,03%, while the infundation using water obtained the yield value of 2,82%. The antioxidant activity test was conducted by DPPH free radical scavenging (1,1-diphenyl-2-phycrilhydrazyl) using a quercetin comparator. The qualitative tests were conducted by TLC method using dicloromethane:methanol eluent with a ratio of 99:1 for methanol extracts, and a ratio of 95:5 for water extracts, where all extracts of both methanol and water positively container antioxidants characterized by the formation of yellow spots with a purple background on TLC plates. The results of the quantitative test shower that the methanol extract had medium antioxidant activity with an IC50 value of 78,39 µg/mL, and water extract had an inactive antioxidant activity with an IC50 value of 319,11 µg/mL. While quercetin was classified as a very strong antioxidant with an IC50 value of 7,62 µg/mL.
Determination of Tannin Content of Gringsingan Stem Extract (Mesosphaerum suaveolens (L) Kuntze) by the UV-Vis Spectrophotometry Handayani, Selpida; Aminah, Aminah; Nurliadi, Indah
Pharmaceutical Reports Vol 4, No 2 (2025): (October) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v4i2.378

Abstract

The Gringsingan plant (Mesosphaerum suaveolens (L.) Kuntze) is widely used in traditional medicine and is known for its diverse pharmacological properties. Traditionally, it has been utilized to treat various ailments such as tumors, malaria, headaches, cancer, fever, stomach pain, yellow fever, rheumatism, constipation, urethritis, diarrhea, and kidney disorders, as well as to act as an analgesic, antiseptic, antispasmodic, and depurative agent. The present study aimed to determine the total tannin content in the stem extract of Gringsingan obtained through Microwave-Assisted Extraction (MAE) and to evaluate its potential as a bioactive compound source. Microwave-assisted extraction was selected because of its advantages, including shorter extraction time, reduced solvent consumption, and efficient recovery of active compounds compared to conventional methods. This experimental research was conducted in the laboratory and consisted of several stages: sample preparation, qualitative testing for tannin presence, and quantitative determination of total tannin content. Qualitative identification using 1% FeCl₃ reagent produced a dark blue to greenish-black coloration, confirming the presence of tannins in the Gringsingan stem extract. The result was further supported by thin-layer chromatography (TLC) testing with the same reagent, which yielded consistent positive results. Quantitative analysis of the total tannin content was carried out using UV–Visible spectrophotometry, with absorbance measured at the maximum wavelength for gallic acid. The analysis revealed that the tannin concentration in the Gringsingan stem extract was 7.487 mg GAE/g extract, indicating that this plant possesses measurable levels of tannins that contribute to its traditional medicinal value.
A Standardisasi Buah Dengen (Dillenia serrata Thumb.) Handayani, Virsa; Putri, Andra Audina; Handayani, Selpida
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 1 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i1.156

Abstract

Dengen (Dillenia serrata Thumb.) merupakan tumbuhan endemik yang tumbuh liar di Sulawesi. Buahnya memiliki beberapa manfaat salah satunya sebagai obat sariawan karena memiliki kadar vitamin C tinggi. Penelitian ini bertujuan mengidentifikasi dan menentukan nilai parameter spesifik dan non spesifik ekstrak buah dengen (Dillenia serrata Thumb.). Sampel diperoleh dari Kecamatan Malili, Kabupaten Luwu Timur Provinsi Sulawesi Selatan, dimaserasi dengan etanol 96% sebanyak 3 L kemudian dilakukan pengujian parameter spesifik dan non spesifik. Penelitian menunjukkan ekstrak buah dengen (Dillenia serrata Thumb.) memberikan hasil sesuai parameter standar umum ekstrak tumbuhan obat dengan nilai parameter spesifik sebagai berikut: ekstrak berbentuk kental, berwarna coklat pekat kehitaman, berbau khas; kadar senyawa larut dalam air yakni 16,0384%; kadar senyawa larut dalam etanol yakni 16,9811%; positif mengandung senyawa alkaloid, polifenol, flavanoid, terpenoid dan saponin; dan nilai parameter non spesifik sebagai berikut: susut pengeringan yakni 0,0615%; kadar air yakni 3,8052%; kadar abu total yakni 5,0599%, kadar abu tidak larut dalam asam yakni 0,4807%.
Uji Aktivitas Sitotoksik Ekstrak Daun Matoa (Pometia pinnata) dengan Metode BSLT (Brine Shrimp Lethality Test) Rahmawati, Junita Eka; Wati, Aulia; Handayani, Selpida
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 1 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i1.159

Abstract

Matoa plant (Pometia pinnata) is a typical Papuan plant included in the Sapindaceae family which can relieve hypertension and has diuretic, antidiabetic and antioxidant activities. Matoa leaves contain chemical alkaloids, flavonoids, saponins, and steroids. The purpose of this study was to determine the toxic effect and LC50 value of n-hexan extract of matoa leaves against Artemia Salina Leach larvae. Extraction is carried out by maceration method with n-hexane solvent. The n-hexan extract of matoa leaves is prepared in a test solution with concentration variations of 500 ppm, 750 ppm, 1000 ppm, and 1500 ppm. Toxicity testing was carried out using Artemia Salina Leach larvae then the toxic effect of the extract was identified with the percentage of death of Artemia Salina Leach larvae using probit analysis (LC50). The results of this study showed the highest mortality at a concentration of 1500 ppm with the death of 30 larvae. Based on the results of the research conducted, it can be concluded that matoa leaf extract (Pometia pinnata) has a toxic effect on Artemia Salina Leach larvae with a Lethal Concentration value of 50 (LC50) of 509.577 ppm and is included in the toxic category.
Identification of Medicinal Chemicals (BKO) Piroxicam in Jamu Pegal Linu Preparations in Makassar City Hasnaeni, Hasnaeni; Handayani, Selpida; Andina, Velda
Hydrogen: Jurnal Kependidikan Kimia Vol. 12 No. 1 (2024): February 2024
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v12i1.10576

Abstract

The aim of the research was to identify Piroxicam in herbal medicine for rheumatic pain circulating in Makassar. There are several traditional medicines that contain medicinal chemicals. Research has been conducted on the identification of medicinal chemicals (BKO) on several rheumatic herbs preparations in herbs shops in Makassar. The BKO which is often included in rheumatic herbs is the NSAID group, one of which is piroxicam. This inspection was conducted to know the presence of the chemical drug piroxicam contained within rheumatic herbs preparations and the samples used were 5 samples. The method used was a qualitative test using thin layer chromatography (TLC) consisting of a mobile phase mixed with n-hexane and acetone (7:3) and a stationary phase using Silica Gel GF254. The results of the TLC examination from 5 samples of herbal medicine added BKO piroxicam contained 3 samples including herbs A, B, and C because they had the same stain color at the same Rf value as the piroxicam. Some of the rheumatic herbs circulating in Makassar contain medicinal chemicals in the form of piroxicam.
Isolation of Endophytic Fungus from Bay Leaves (Syzygium polyanthum Wight.) as Antibacterials Against Bacteria Causing Digestive Tract Infections by Bioautography-TLC Fahirah, Jihan; Herwin, Herwin; Handayani, Selpida
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1301

Abstract

Salam (Syzygium polyanthum (Wight) Walp), belonging to the Myrtaceae family, are noted for their potential antibacterial properties targeting bacteria linked to gastrointestinal infections. This study aims to identify endophytic fungus isolates from bay leaves that produce antibacterial compounds effective against bacteria responsible for gastrointestinal infections, utilizing TLC-Bioautography as a method of analysis. The endophytic fungus from bay leaves was isolated, yielding 17 pure isolates. The findings from the antagonist test of pure isolates of endophytic fungus revealed two isolates with the most significant inhibitory zone diameters: IFSP-8 and IFSP-15. These isolates demonstrated effectiveness against Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholerae bacteria. The active isolate was then extracted by liquid-liquid extraction to obtain ethyl acetate extract for the IFSP-8 and IFSP-15 isolates. The antibacterial activity results of the ethyl acetate extract, analyzed through TLC-Bioautography with a chloroform: methanol (9:1) eluent, indicated the values of Rf1 0,76; Rf2 0,56; Rf3 0,47 for the IFSP-8 isolate. In contrast, the IFSP-15 isolate exhibited the values of Rf1 0,81; Rf2 0,69; Rf3 0,56, demonstrating inhibition of bacteria responsible for gastrointestinal infections.
Quantification of Flavonoid Compounds in Keji Beling Leaves (Strobilanthes Crispa (L.) Blume) Extracted by Ultrasonic Assisted Extraction (UAE) and Maceration Using TLC Densitometry Hasnaeni; Handayani, Selpida; Utami, Fadhillah Resky; Majid, Nini Nirmala
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 5 (2025): October 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i5.17748

Abstract

Keji Beling leaves (Strobilanthes crispa (L.) Blume) contain secondary metabolites such as flavonoids, tannins, saponins, alkaloids, and polyphenols. These secondary metabolites have antioxidant, antidiabetic, and anticancer activities. In this study, flavonoid identification was tested using thin-layer chromatography (TLC), followed by determination of the flavonoid content in Keji Beling leaf extract (Strobilanthes crispa (L.) Blume) using the densitometry method. The extraction methods used were Ultrasonic Assisted Extraction (UAE) and maceration using 70% ethanol solvent, yielding extracts with a yield of 15.143% and 11.092%, respectively.Quantification of flavonoid compounds using quercetin standard showed that the average flavonoid content of keji beling leaf extract using the Ultrasonic Assisted Extraction (UAE) method was 0.057 mg/mL and using the maceration method was 0.156 mg/mL. This study aims to determine the flavonoid content of keji beling extract using the TLC densitometry method.    
Phytochemical Screening of Strobilanthes crispa Leaf Extract Obtained by Ultrasound-Assisted Extraction (UAE) Method Hasnaeni; Nurlina; Handayani, Selpida; Anasya, Andi Fara; Wenni, Asyam Hafidz
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 6 (2025): December 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i6.18483

Abstract

The study was conducted to determine  of secondary metabolite compounds in the ethanol extract of keji beling leaves (Strobilanthes crispa (L.). Keji beling leaves are known to possess pharmacological effects such as antioxidant, antibacterial, and anticancer. These pharmacological effects are due to the presence of secondary metabolites. This study aims to investigate the secondary metabolite compounds contained in keji beling (S. crispa). Fresh keji beling leaves were dried, ground into powder, and extracted using 70% ethanol solvent using the Ultrasonic Assisted Extraction (UAE) method. Phytochemical screening was carried out by adding reagents specific for secondary metabolites. The test results showed that the ethanol extract of keji beling leaves contained positive flavonoids, alkaloids, saponins, and tannins, while the terpenoid test showed negative results. The presence of these metabolites indicates that keji beling leaves have the potential as a source of bioactive compounds and can be developed into raw materials for herbal medicine preparations.
Karakteristik Fitosom dari Ekstrak Etanol Daun Laruna (Chromolaena odorata L.) menggunakan SEM (Scanning Electron Microscope) dan PSA (Particle Size Analyzer) Tahir, Karlina Amir; Syahrana, Nur Azizah; Putri, Aulia Dinda; Annisa, Khairun; Imani, Fitril; Indah, Indah; Handayani, Selpida; Nonci, Faridha Yenni; Lalo, Ahmad
Jurnal Mandala Pharmacon Indonesia Vol. 11 No. 2 (2025): Jurnal Mandala Pharmacon Indonesia 
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v11i2.816

Abstract

Daun laruna (Chromolaena odorata L.) mengandung senyawa utama seperti saponin, fenol, tanin, steroid dan flavonoid. Flavonoid dalam daun laruna memiliki potensial terapetik yang luas, salah satunya sebagai antioksidan, senyawa fenolik yang ada dalam flavonoid memiliki polaritas yang tinggi sehingga menyebabkan bioavailabilitasnya rendah. Fitosom merupakan suatu sistem penghantaran yang dibentuk oleh bahan aktif alami dan fosfatidilkolin. Sistem fitosom diketahui dapat membantu untuk meningkatkan bioavailabilitas bahan aktif. Tujuan dari penelitian ini yaitu membuat Drug Delivery System nanopartikel dengan sistem vesikular yaitu fitosom dari ekstrak daun laruna. Tahapan pertama yang dilakukan yaitu ekstraksi yang dilakukan dengan metode maserasi dengan pelarut etanol 70%. Pembuatan fitosom pada pengujian ini menggunakan metode dispersi mekanik dengan pembawa fosfatidilkolin dan pelarut aseton. Dari hasil fitosom kemudian dilakukan karakteristik morfologi fitosom menggunakan SEM (Scanning Electron Microscope) dan pengukuran partikel fitosom menggunakan PSA (Particle Size Analyzer). Hasil uji morfologi fitosom menggunakan SEM menunjukkan bahwa fitosom yang dibuat telah memenuhi spesifikasi morfologi yaitu berbentuk bulat padatan (spheris), sedangkan hasil untuk ukuran partikel fitosom menggunakan PSA daun laruna didapatkan hasil nilai rata-rata sebesar 105.1 nm, dan untuk nilai indeks polidispersitas sebesar 0.299. Dari hasil penelitian dapat disimpulkan bahwa ekstrak daun laruna dapat dikembangkan menjadi sistem fitosom dengan karakteristik yang baik.
Co-Authors Abdul Rasyid, Faradiba Amaliah Dahlia, Andi Aminah Aminah Amran, Farizah Dhaifina Anasya, Andi Fara Andina, Velda Annisa, Khairun Aulia Wati, Aulia Badu, Nurul Fadillah Fahirah, Jihan Faradiba Hasnaeni Hasnaeni hasnaeni, Hasnaeni Herwin Herwin, Herwin Hossani, Rahni Ida Kurniawati, Ida Imani, Fitril Indah Indah Indah Pratiwi Lalo, Ahmad Majid, Nini Nirmala Mamat Pratama, Mamat Nonci, Faridha Yenni Nurliadi, Indah Nurlina Nurlina Nurlina Putri, Andra Audina Putri, Aulia Dinda Rahmawati, Junita Eka Rais Razak, Rais Rezki Amriati Syarif, Rezki Amriati Risda Waris, Risda Sari, Felisa Ananda Syahrana, Nur Azizah Tahir, Karlina Amir Utami, Fadhillah Resky Virsa Handayani Wenni, Asyam Hafidz Wisdawati Wisdawati, Wisdawati