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All Journal Mutiara Medika: Jurnal Kedokteran dan Kesehatan Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Syntax Literate: Jurnal Ilmiah Indonesia YARSI Medical Journal Medicra (Journal of Medical Laboratory Science/Technology) Cermin Dunia Kedokteran Junior Medical Journal Journal of Global Pharma Technology Indonesian Journal of Endocrinology Metabolism and Diabetes (InaJEMD) Cermin Dunia Kedokteran
Fatimah Eliana
Faculty Of Medicine, Universitas YARSI
Religion Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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Isolation, In Vitro Expansion, And Cryopreservation of Primary Cells Derived from Human Thyroid Carcinoma Fatimah Eliana; Sabrina Azmi; Anggi Puspa Nur Hidayati; Juhan Khalila
‎ InaJEMD - Indonesian Journal of Endocrinology Metabolism and Diabetes Vol. 2 No. 1 (2025): InaJEMD Vol. 2, No. 1
Publisher : PP PERKENI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.66266/inajemd.v2i1.35

Abstract

Thyroid carcinoma is a malignant neoplasm arising from thyroid parenchymal cells and currently ranks as the fourth most diagnosed cancer in Indonesia. This study aimed to isolate thyroid carcinoma cells for in vitro expansion and long-term preservation as a reliable cell culture stock, including cryopreservation for future research applications. In addition, we sought to identify and characterize cells derived from papillary thyroid carcinoma (PTC) tissue to evaluate the presence of mutations with potential prognostic significance. Primary cell isolation was performed via enzymatic digestion using collagenase, enabling effective separation of tumor cells from adjacent non-malignant thyroid tissue. Cell proliferation was supported using Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 20% fetal bovine serum (FBS), selected for its high concentration of growth-promoting factors that enhance proliferation rates. For biobanking purposes, cryopreservation of the thyroid carcinoma-derived cells was conducted using a standard slow-freezing protocol. Molecular characterization was carried out through PCR amplification, gel electrophoresis, and Sanger sequencing of key oncogenic drivers, specifically the BRAF gene and five RAS gene targets: HRAS exon 2, NRAS exons 2 and 3, and KRAS exons 2 and 3. No pathogenic mutations were identified in the analyzed BRAF or RAS gene regions.
Co-Authors Achya, Vioni Alvida Alida Harahap Alyka Sheila Masah Amir Mahmud Andri Gunawan Anggi Puspa Nur Hidayati Anshar, Fazlurrahman Arini Setiawati Arini Setiawati Asmarinah Asmarinah Citra Fitri Citra Fitri Agustina Dante Saksono Harbuwono Dewi, Lia Sari Utami Diah Ambarwati, Fransisca Diniwati Mukhtar Dwi Anita Suryandari Dwi Yanti Farah Zahida Fransisca Diah Ambarwati Fransisca Diah Ambarwati Gunawan, Andri Hakim Makmun, Lukman Hakim, Jasir Handoko, Iwan Surjadi Iwan Surjadi Handoko Iwan Surjadi Handoko Joedo Prihartono Juhan Khalila Juhan Khalila Legita Suci Luthfia Luluk Yunaini Maliki, Khaira Mochammad Imron Awalludin Muchtar, Diniwati Muhammad Arsyad Naya, Nur Afifah Inayah Pradana Suwondo Restu Syamsul Hadi Rufi'i Sabrina Azmi Samsuridjal Djauzi Shaffdika, Kyra Narumi Najwa Sherly Idhalhijah Syifa Aziza Syifa Tjokorda Gde Dalem Pemayun Trisia Lusiana Amir Widiastari, Erina Febriani Zahida, Farah