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Rosnina Rosnina, Rosnina
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Isolasi dan Identifikasi Cendawan dari Pelapukan Batang Bawah Sagu (Metroxylon sagu Rottb.): Isolation and Indentification of Fungi from Weathering Sago Rootstock (Metroxylon sagu Rottb.) Ilyas Mattola, Paradillah; Naima Haruna; Rosnina, Rosnina
Perbal: Jurnal Pertanian Berkelanjutan Vol. 13 No. 1 (2025): Perbal: Jurnal Pertanian Berkelanjutan
Publisher : Fakultas Pertanian Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/perbal.v13i1.4628

Abstract

Pelapukan batang bawah sagu merupakan proses alami yang terjadi pada bagian batang sagu yang tidak lagi aktif secara fisiologis. Berbagai mikroorganisme, diantaranya adalah cendawan, bakteri, nematoda dan organisme tanah lainnya, memakan bahan organik dalam proses pelapukan ini. Pentingnya pelapukan batang sagu dalam siklus ekosistem, terutama dalam pengembalian nutrisi ke dalam tanah. Penelitian ini bertujuan untuk mengisolasi cendawan yang terdapat pada pelapukan batang bawah sagu serta melakukan identifikasi cendawan yang ditemukan dari hasil pelapukan batang bawah sagu. Penelitian ini dilaksanakan pada bulan Juni - September 2024 di Desa Pengkajoang, Kecamatan Malangke Barat Kabupaten Luwu Utara. Penelitian ini dilaksanakan dengan mengambil sampel hasil pelapukan batang bawah sagu pada kedalaman 15 cm. Sampel diambil dari lima titik dengan jumlah 100 gram pada setiap titik. Pengambilan sampel dilakukan dengan prosedur yang sama di setiap titik. Sampel kemudian diisolasi menggunakan metode pengenceran berseri, di mana 1 gram sampel dilarutkan dalam 9 ml air steril dan dikocok menggunakan sentrifugasi selama 30 menit hingga tercampur rata. Selanjutnya, 1 ml suspensi diambil dan dimasukkan ke dalam tabung reaksi berisi 9 ml aquades steril. Pengenceran berseri dilakukan hingga tercapai pengenceran 104. Sampel dari pengenceran 101, 102, dan 103, sebanyak 0,5 ml suspensi, ditumbuhkan pada media PDA menggunakan metode sebar dan diinkubasi selama 3 hingga 7 hari pada suhu 22-25°C. Identifikasi isolat cendawan dilakukan menggunakan kunci determinasi dari Illustrated Genera of Imperfect Fungi (Barnett & Hunter, 1998). Penelitian ini menemukan empat genus cendawan, yaitu Trichoderma, Penicillium, Gliocladium, dan Rhizopus. Weathering sago rootstock is a natural process natural occurs in parts of the sago stem that are no longer physiologically active. Various microorganisms, including fungi, bacteria, nematodes, and other soil organisms, eat organic material in this weathering process. The importance of weathering sago stems in the ecosystem cycle, especially in returning nutrients to the soil. This research aims to isolate fungi found in weathering sago rootstocks and identify fungi found in the weathering of sago rootstocks. This research was carried out in June - November 2024 in Pengkajoang Village, West Malangke District, North Luwu Regency. This research was carried out by taking samples from the weathering of sago rootstock at a depth of 15 cm. Samples were taken from five points, with 100 grams collected from each point. The sampling procedure was consistent across all points. The samples were then isolated using a serial dilution method, where 1 gram of the sample was dissolved in 9 ml of sterile water and shaken using centrifugation for 30 minutes until homogenized. Subsequently, 1 ml of the suspension was taken and placed into a test tube containing 9 ml of sterile aquades. The serial dilution was carried out until a 104 dilution was achieved. Samples from dilutions 101, 102, and 103, 0.5 ml each, were then cultured on PDA media using the spread plate method and incubated for 3 to 7 days at 22-25°C. Fungal isolates were identified using a determination key from Illustrated Genera of Imperfect Fungi (Barnett & Hunter, 1998). The study identified four fungal genera: Trichoderma, Penicillium, Gliocladium, and Rhizopus.
Pengaruh Jenis Mulsa Dan Aplikasi Pupuk Organik Cair Terhadap Pertumbuhan Dan Hasil Tanaman Kentang (Solanum tuberosum L.) Ismadi, Ismadi; Indri Yani, Nurul; Hafifah, Hafifah; Rosnina, Rosnina; Nazaruddin, M
Agrium Vol 18 No 1 (2021)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v18i1.3846

Abstract

Tanaman kentang merupakan salah satu komoditi hortikultura yang berpotensi dikembangkan karena dapat karena memiliki protein, vitamin dan mineral yang tinggi serta mempunyai peran yang penting bagi perekonomian indonesia. Tanaman kentang merupaka tanaman sayuran  berumur pendek. Penelitian ini bertujuan untuk mengetahui pengaruh jenis mulsa dan aplikasi pupuk organik cair terhadap pertumbuhan dan hasil tanaman kentang. Penelitian ini dilaksanakan di desa Ujung Gele Kecamatan Bukit Kabupaten Bener Meriah dengan ketinggian tempat 1300 mdpl. Penelitian ini dilaksanakan mulai Juli sampai November 2020. Penelitian ini menggunakan rancangan acak kelompok (RAK) faktorial dengan dua faktor. Faktor pertama adalah jenis mulsa yang terdiri atas tiga taraf yaitu tanpa mulsa (M1), mulsa plastik hitam perak (M2), dan mulsa kulit biji kopi (M3). Fakotr kedua adalah pupuk organik cair yang terdiri atas tiga taraf yaitu kontrol (P0), 4 ml/l air (P1), dan 8 ml/l air (P2). Peubah yang diamati adalah tinggi tanaman, jumlah daun, jumlah batang, jumlah umbi per sampel, jumlah umbi per plot, berat umbi per sampel, dan berat umbi per plot. Hasil penelitian menunjukkan bahwa pada perlakuan jenis mulsa hasil terbaik diperoleh dari penggunaan mulsa plastik hitam perak dan perlakuan terbaik pupuk organik cair adalah pada konsentrasi 8 ml/l air. Tidak didapati interaksi antara perlakuan jenis mulsa dan pupuk organik cair terhadap semua peubah yang diamati. Kata kunci; granola, umbi, batang, daun 
Rehabilitation of Former Nickel Mine Soil through Humate and Compost Application for Revegetation Plant Growth Media Sapareng, Sukriming; Erwina, Erwina; Arzam AR, Taruna Shafa; Boceng, Annas; Akmal, Akmal; Rosnina, Rosnina; Ilyas M, Paradillah; Suryanto, Suryanto
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.10163

Abstract

Former nickel mine lands often have low soil quality and do not support the growth of revegetation plants. This study aims to evaluate the effect of humic substances and compost on improving the quality of post-nickel mine soil and plant growth media. The experiment was conducted using a completely randomized design in a factorial pattern with three levels of humic and compost treatments, as well as a combination of both, applied to nickel post-mining soil. The results showed that the addition of compost significantly increased the cation exchange capacity (CEC) and availability of nutrients (Ca, Mg, K, Na) in the soil. The addition of humic materials had relatively no significant impact on increasing CEC, but could increase phosphorus levels in the soil. In addition, compost and the combination of compost with humic materials can reduce available heavy metal Cr, although it causes an increase in Ni heavy metal levels in the soil. The results of this study make a significant contribution to the rehabilitation of ex-mining land in a sustainable manner, especially the use of humic materials and compost as a strategy to improve soil quality and increase agricultural productivity. The use of these organic materials is proven to be effective in improving the physical, chemical and biological properties of degraded soils, so it can be a more environmentally friendly approach compared to the use of synthetic chemicals
Co-Authors Akmal Akmal Akram, Nuzul Anggraini, Aqsyah Annas Boceng Arief Rahman Baidhawi Baidhawi Dewi Marwati Nuryanti, Dewi Marwati Erwina Erwina, Erwina Eva Wardah Hafifah, Hafifah Hafifah, Hafifah Halid, Irwan Heni Pujiastuti Ilyas M, Paradillah Ilyas Mattola, Paradilla Ilyas Mattola, Paradillah Indri Yani, Nurul Ismadi Ismadi Kesia, Kesia Keumala, Ida Khaidir Khaidir Laila Nazirah Laksono Trisnantoro Martina Martina Mattola, Paradillah Ilyas Mauliza, Sarah Mawardati Mawardati Mudaffar, Rahmi Azizah Naima Haruna Naima Haruna, Naima Nasruddin Nasruddin Nurdin, Muhammad Yusuf Riani Riani, Riani SATRIYAS ILYAS Suryanto Suryanto Taruna Shafa Arzam AR, Taruna Shafa