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Production of Clarias gariepinus seeds through accelerated female broodstock rematuration Prama Hartami; Mahdaliana Mahdaliana; Eva Ayuzar
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

This study aims to determine the effectiveness of Oodev and Turmeric flour on feed to accelerate the rematuration of female broodstock catfish for increasing seed production. Oodev hormone is believed to accelerate the maturation process of broodstock, while turmeric flour with its active ingredients can improve liver performance in the vitellogenesis process. The method was a non-factorial completely randomized design with 4 treatments and 3 replications. The treatments are Control, (A) Oodev 0.3 ml/kg of feed, (B) Turmeric flour 5 gr/kg of feed, and (C) Combination of Oodev + Turmeric flour. The results showed that C treatment was the best treatment which produced a GSI value of 14.57 ±2.53%, period of rematuration was 32.33 ±4.16 days, egg diameter was 1.27 ±0.12 mm, fecundity was 22,939.97 ±880.38 eggs/kg of broodstock weight, fertilization rate was 80.18 ±9.75%, hatching rate was 79.80 ±2.88%, and larval survival reached 83.17 ±9.69%. Meanwhile, the specific growth of seed length in all treatments had no significant effect between treatments. The results of this study will certainly provide useful information for increasing catfish seed production both in Aceh and on a Nationally. This acceleration of maturation also has a positive impact in maximizing the available broodstock without having to increase the number of catfish broodstock to produce seeds of superior quality and benefit the cultivators.Keywords:EnrichmentFemale BroodstockPerformanceRematurationReproduction

Production of Clarias gariepinus seeds through accelerated female broodstock rematuration Prama Hartami; Mahdaliana Mahdaliana; Eva Ayuzar
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22860

This study aims to determine the effectiveness of Oodev and Turmeric flour on feed to accelerate the rematuration of female broodstock catfish for increasing seed production. Oodev hormone is believed to accelerate the maturation process of broodstock, while turmeric flour with its active ingredients can improve liver performance in the vitellogenesis process. The method was a non-factorial completely randomized design with 4 treatments and 3 replications. The treatments are Control, (A) Oodev 0.3 ml/kg of feed, (B) Turmeric flour 5 gr/kg of feed, and (C) Combination of Oodev + Turmeric flour. The results showed that C treatment was the best treatment which produced a GSI value of 14.57 ±2.53%, period of rematuration was 32.33 ±4.16 days, egg diameter was 1.27 ±0.12 mm, fecundity was 22,939.97 ±880.38 eggs/kg of broodstock weight, fertilization rate was 80.18 ±9.75%, hatching rate was 79.80 ±2.88%, and larval survival reached 83.17 ±9.69%. Meanwhile, the specific growth of seed length in all treatments had no significant effect between treatments. The results of this study will certainly provide useful information for increasing catfish seed production both in Aceh and on a Nationally. This acceleration of maturation also has a positive impact in maximizing the available broodstock without having to increase the number of catfish broodstock to produce seeds of superior quality and benefit the cultivators.Keywords:EnrichmentFemale BroodstockPerformanceRematurationReproduction

Production of Clarias gariepinus seeds through accelerated female broodstock rematuration Prama Hartami; Mahdaliana Mahdaliana; Eva Ayuzar
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22860

This study aims to determine the effectiveness of Oodev and Turmeric flour on feed to accelerate the rematuration of female broodstock catfish for increasing seed production. Oodev hormone is believed to accelerate the maturation process of broodstock, while turmeric flour with its active ingredients can improve liver performance in the vitellogenesis process. The method was a non-factorial completely randomized design with 4 treatments and 3 replications. The treatments are Control, (A) Oodev 0.3 ml/kg of feed, (B) Turmeric flour 5 gr/kg of feed, and (C) Combination of Oodev + Turmeric flour. The results showed that C treatment was the best treatment which produced a GSI value of 14.57 ±2.53%, period of rematuration was 32.33 ±4.16 days, egg diameter was 1.27 ±0.12 mm, fecundity was 22,939.97 ±880.38 eggs/kg of broodstock weight, fertilization rate was 80.18 ±9.75%, hatching rate was 79.80 ±2.88%, and larval survival reached 83.17 ±9.69%. Meanwhile, the specific growth of seed length in all treatments had no significant effect between treatments. The results of this study will certainly provide useful information for increasing catfish seed production both in Aceh and on a Nationally. This acceleration of maturation also has a positive impact in maximizing the available broodstock without having to increase the number of catfish broodstock to produce seeds of superior quality and benefit the cultivators.Keywords:EnrichmentFemale BroodstockPerformanceRematurationReproduction

Implementasi Pretreatment Ekstraksi Lipid untuk Produksi Biodiesel Nannochloropsis sp. yang Dikultivasi dalam Media Limbah Budidaya Vaname Rusydi, Rachmawati; Alfaini, Momika; Ayuzar, Eva; Hartami, Prama; Salamah, Salamah; Muliani, Muliani; Khalil, Munawwar; Febri, Suri Purnama
Jurnal Ilmiah Samudra Akuatika Vol 8 No 1 (2024): Jurnal Ilmiah Samudra Akuatika
Publisher : Fakultas Pertanian Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jisa.v8i1.10831

Nannochloropsis sp. is the green colored microalgae with its round shape of cell. This microalgae has lipids of 28,7% by dry weight, especially unsaturated fatty acids which can be transesterified to produce methyl ester and be a potential as biodiesel feedstock. The content of biodiesel Nannochloropsis sp. reaches 80% of dry weight and has the sama characteristics as vegetable oil. In the lipid extraction process many problems were found, one of which was the cell wall of Nannochloropsis sp. which prevents the lipid from being extracted. This research was carried out on 10 December 2022-28 February 2023 at the Hatchery and Aquaculture Technology Laboratory aquaculture study program, faculty of agrigulture, Malikussaleh University, Chemical Technology Laboratory at Lhokseumawe State Polytechnic and at The Environmental Quality Testing Engineering Laboratory At Syiah Kuala University, Banda Aceh. This study used a laboratory experimental method using a Non-Factorial Completely Randomized Design (CRD) consisting of 4 treatments and 3 replications. Treatment A Control, treatment B Heating, treatment C Osmotic shock and treatment D Homogenizer. Based on statistical analysis of the F test (ANOVA) showed that the percentage of lipid from the application of pretreatment gave significantly different resulst to the percentage of lipid from the application of pretreatment. The highest lipid was found in treatment B which was 21,14% and the lowest lipid was in treatment C which was 12,51%. The highest lipid producer from the application of pretreatment is using the heating method. Biodiesel is obtained through a tranesterification process using methanol as a solvent and KOH catalyst.

UJI KETAHANAN PENYAKIT MOTILE AEROMONAS SEPTICEMIA (MAS) MENGGUNAKAN BAKTERI Aeromonas hydrophila PADA IKAN PATIN TRIPLOID Hartami, Prama
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 20, No 1 (2024): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.20.1.%p

Motile Aeromonas Hydrophila (MAS) disease caused by Aeromonas hydrophila is a common pathogen that attacks freshwater fish commodities, including triploid as the new strain of striped catfish. The purpose of this study was to test the resistance of triploid catfish to MAS infection before being released to cultivators as a new commodity to increase national striped catfish production. The test was carried out for 2 months at the Sukamandi Fish Breeding Research Institute, Subang by injecting A. hydrophila bacteria into triploid and diploid catfish. The challenge test was carried out by injecting the cultured A. hydrophila bacteria with a density of 107 cfu.mL-1 as much as 0.1 mL.tail-1 at fish intramuscularly in all treatments, while in the Control (-) treatment, 0.1 mL of PBS solution was injected.tail-1. The test striped catfish used for each treatment were 10 tails measuring 9.23 – 9.65 cm, and weighing 7.1 – 9.23 g. Survival parameter data and blood picture were analyzed quantitatively using Microsoft Excel 2010 and SPSS version 16.0 software with analysis of variance (ANOVA) at 95% confidence interval. The results of re-characterization with biochemical tests showed that A. hydrophila bacteria were gram negative, oxidase and catalase positive, motile, could fermentative convert sugar and could grow in blood media. The survival of triploid striped catfish after the test was 95%, total erythrocytes on day 7, and total leukocytes on the first day were significantly different from diploid striped catfish. Thus, it can be concluded that striped triploid catfish are more resistant to infection with A. hydrophila bacteria than diploid.

Implementation of Pretreatment in Lipid Extraction to Biodiesel Production from Nannochloropsis sp Cultivated in Vannamei Farming Wastewater Rusydi, Rachmawati; Alfaini, Momika; Ayuzar, Eva; Hartami, Prama; Salamah, Salamah; Muliani, Muliani; Khalil, Munawwar; Febri, Suri Purnama
Jurnal Ilmiah Samudra Akuatika Vol 8 No 1 (2024): Jurnal Ilmiah Samudra Akuatika
Publisher : Fakultas Pertanian Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jisa.v8i1.10905

Nannochloropsis sp. is the green colored microalgae with its round shape of cell. This microalgae has lipids of 28,7% by dry weight, especially unsaturated fatty acids which can be transesterified to produce methyl ester and be a potential as biodiesel feedstock. The content of biodiesel Nannochloropsis sp. reaches 80% of dry weight and has the sama characteristics as vegetable oil. In the lipid extraction process many problems were found, one of which was the cell wall of Nannochloropsis sp. which prevents the lipid from being extracted. This research was carried out on 10 December 2022-28 February 2023 at the Hatchery and Aquaculture Technology Laboratory aquaculture study program, faculty of agrigulture, Malikussaleh University, Chemical Technology Laboratory at Lhokseumawe State Polytechnic and at The Environmental Quality Testing Engineering Laboratory At Syiah Kuala University, Banda Aceh. This study used a laboratory experimental method using a Non-Factorial Completely Randomized Design (CRD) consisting of 4 treatments and 3 replications. Treatment A Control, treatment B Heating, treatment C Osmotic shock, and treatment D Homogenizer. Based on statistical analysis of the F test (ANOVA) showed that the percentage of lipid from the application of pretreatment gave significantly different resulst to the percentage of lipid from the application of pretreatment. The highest lipid was found in treatment B which was 21,14% and the lowest lipid was in treatment C which was 12,51%. The highest lipid producer from the application of pretreatment is using the heating method. Biodiesel is obtained through a tranesterification process using methanol as a solvent and KOH catalyst.

Pendampingan Kelompok Maju Bersama dalam Upaya Memproduksi Benih Ikan Lele Mutiara (Clarias sp) Hartami, Prama; Nirzalin, Nirzalin; Martina, Martina; Hatta, Muhammad; Rafi, Muhammad; Zuraida, Zuraida; Rizki, Muhammad
INTEGRITAS : Jurnal Pengabdian Vol 7 No 2 (2023): AGUSTUS - DESEMBER
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v7i2.2285

Kegiatan pembenihan merupakan upaya yang dilakukan untuk memproduksi benih agar tidak terjadi ketergantungan stok pada suplier benih, sehingga kegiatan pembesaran ikan lele tidak mengalami kendala produksi yang diakibatkan oleh keterbatasan benih atau mahalnya harga benih untuk ukuran tertentu. Hal inilah yang dilakukan oleh Tim Pengusul kepada Mitra yang tergabung dalam Kelompok Usaha Maju Bersama di Gampong Blang Kubu, Kecamatan Peudada, Kabupaten Bireuen. Tujuan pengebdian ini adalah untuk membantu kelompok mitra agar mampu menyediakan benih secara mandiri untuk kebutuhan pembudidayaan ikan lele bagi para anggota dan masyarakat sekitar yang tertarik untuk terlibat dalam usaha pembesaran ikan lele. Kegiatan Pengabdian ini dilaksanakan mulai tanggal 05 September hingga 05 November 2022 menggunakan metode deskriptif kualitatif pada kegiatan pembenihan ikan lele mutiara melalui induced spawning. Tahapan kegiatan pendampingan ini meliputi hal-hal seperti penyediaan induk unggul, persiapan wadah, perawatan induk, seleksi induk, dan proses pemijahan yang dilakukan. Hasil pendampingan khusus kepada mitra didapatkan bahwa banyak dukungan penuh yang diberikan kepada tim pelaksana agar target yang ingin dicapai bisa terlaksana secara optimal, hal ini terlihat dari banyak fasilitas yang dimiliki mitra dapat secara penuh dimanfaatkan oleh tim pengusul.

Pemberdayaan Masyarakat di Gampong Paloh Lada untuk Optimalisasi Produksi Kepiting Bakau dan Ikan Berbasis Tingkat Tropik di Masa Pandemi Covid 19 Hartami, Prama; Nirzalin, Nirzalin; Khalil, Munawwar
Jurnal Malikussaleh Mengabdi Vol. 1 No. 1 (2022): Jurnal Malikussaleh Mengabdi, April 2022
Publisher : LPPM Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/jmm.v1i1.6536

Pandemi Covid 19 yang melanda seluruh dunia memberikan dampak yang signifikan bagi masyarakat terutama kesulitan dalam hal memenuhi kebutuhan ekonomi keluarga. Hal ini dikarenakan adanya kebijakan pemerintah baik pusat maupun daerah untuk membatasi aktivitas masyarakat keluar rumah. Pembatasan ini sangat dirasakan oleh masyarakat yang tidak memiliki pekerjaan tetap yang kesehariannya harus berinteraksi dengan orang banyak untuk dapat menghasilkan uang guna memenuhi kebutuhan hariannya. Tujuan dari kegiatan pendampingan ini ialah untuk memberikan kesempatan dan peluang usaha bagi masyarakat terdampak Pandemi Covid 19 dan bagi pemilik lahan program ini bertujuan sebagai sharing informasi baru dalam memaksimalkan lahan tambak yang ada dengan sistem Trophic Level Based Aquaculture. Tahap pelaksanaannya dengan memilih satu komoditas yang bernilai ekonomis tinggi dengan periode produksi yang cepat dan disertai dengan memelihara komoditas lain sebagai pendukung tetapi tidak saling mengganggu. Sehingga pada saat panen, pembudidaya dapat memproduksi lebih dari satu atau bahkan empat komoditas untuk dijual di pasar sehingga mampu memberikan tambahan penghasilan. Adapun metode pendampingan ini bersifat learning by doing artinya semua yang terlibat dalam pendampingan mempraktekkan sistem budidaya tersebut secara bersama-sama di lapangan. Program ini akan dimulai dengan penetapan peserta yang akan dilakukan pendampingan, pemilihan lahan potensial yang dimiliki masyarakat petambak, pelatihan teknis budidaya dengan sistem Trophic Level Based Aquaculture, persiapan lahan, pemilihan komoditas unggulan, pemeliharaan komoditas hingga panen, pemasaran dan manajemen modal usaha untuk periode pemeliharaan selanjutnya. Hasil dari program pendampingan ini diharapkan bisa menjadi bench marking bagi calon pembudidaya dan pemilik lahan lainnya yang ada di sekitar lokasi kegiatan. Selain metode pelaksanaan, kegiatan ini juga diharapkan mampu memaksimalkan lahan budidaya yang sebelumnya dikelola secara tradisional menjadi lebih optimal serta ramah lingkungan guna mencapai target budidaya yang berkelanjutan. Hasil kegiatan pendampingan ini akan disebarluaskan melalui publikasi ilmiah pada jurnal pengabdian masyarakat, media online atau disajikan pada seminar nasional dan laporan pengabdian yang akan didaftarkan pada HKI.

Pengaruh penambahan bahan pengencer sperma terhadap fertilitas spermatozoa ikan lele dumbo (Clarias gariepinus) Hartami, Prama; Nur, Muhammad; Ayuzar, Eva
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Aquatic Sciences Journal, Vol. 1: No. 1 (October, 2014)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v1i1.298

Penelitian ini dilaksanakan selama 9 hari yang bertempat di Laboratorium Hatcheri dan Teknologi Budidaya Universitas Malikussaleh, Aceh Utara,Propinsi Aceh. Penelitian ini bertujuan untuk mengatahui pengaruh penambahan bahan pengencer sperma terhadap fertilitas spermatozoa ikan lele dumbo dan memperoleh jenis bahan pengencer yang terbaik dalam pemijahan ikan lele dumbo. Penelitian ini di gunakan metode eksperimen laboratorium yang di gunakan pola rancangan acak lengkap (RAL) non faktorial dengan 4 perlakuan 3 ulangan. Bahan uji yang digunakan adalah spermatozoa ikan lele dumbo, Ovaprim, Akuadest, Susu steril, Air kelapa muda, Larutan Infus/ NaCl. Parameter yang di ukur ialah daya fertilitas telur, derajat penetasan telur, kelangsungan hidup benih. Berdasarkan hasil penelitian menunjukkan bahwa penggunaan Aquades sebagai pengencer merupakan bahan yang terbaik dimana fertilitas didapatkan sebesar 98.33 %, derajat penetasan telur sebesar 77.28 %, tingkat kelangsungan hidup larva sebesar 93.44 %. Hasil uji analisis statistik untuk semua parameter uji menunjukkan F hitung > dari F Tabel untuk perlakuan Aquades sebagai bahan pengencer. Sementara itu, hasil pengukuran parameter kualitas air seperti suhu 26,07 - 28,15 0C dan pH air 8,67 - 8,95.This research was done for 9 days in Laboratory of Hatchery and Aquaculture Technology Malikussaleh University, North Aceh, Aceh Province. The aim of this research was to know effect of different sperm solutions for effectiveness fertilization rate of Clarias gariepinus. This research used non factorial completely randomized treatment with three replications on each treatments that using aquadest, natural milk, coconut water and NaCl fisiologis as the sperm solutions. The test observed in this research was fertilization rate, hatching rate, and survival rate. Result showed us that the used of aquadest as the sperm solutions got the best goals which was 98,33 % for ferlilization rate, 77,28 % for hatching rate, and 93,44 % for survival rate. Based on statistical analyze also showed that f count > f table in 99.99 % level of trust for the first treatment with value 29.417 > 7.59. Meanwhile the water quality value that measurable during this research was 26.07 - 28.15 0C and 8.67 - 8.95 for pH.

Karakteristik nutrisi dan stabilitas pakan kombinasi ampel (ampas tahu dan pelet) Rusydi, Rachmawati; Hartami, Prama; Khalil, Munawwar
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Aquatic Sciences Journal, Vol. 4: No. 1 (April, 2017)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v4i1.316

Tingginya biaya produksi akibat dari mahalnya harga pakan berprotein tinggi menyebabkan eksplorasi bahan pakan alternatif yang bernutrisi, murah dan tersedia sepanjang tahun terus dilakukan. Penelusuran bahan alternatif ini juga diharapkan dapat meningkatkan kandungan protein dari pakan komersil berprotein rendah yang murah. Metode penelitian terdiri atas tahap pembuatan pakan uji, analisis proksimat, dan uji ketahanan pakan di air. Adapun perlakuan dalam penelitian ini adalah: (A) kombinasi ampas tahu 80% + pelet 20%, (B) kombinasi ampas tahu 60% + Pelet 40%, (C) kombinasi ampas tahu 40% + pelet 60%, (D) kombinasi ampas tahu 20% + pelet 80%, (E) kontrol (penggunaan pelet 100%). Hasil penelitian menunjukkan bahwa pakan ampel dengan kombinasi ampas tahu 40% + pelet 60% memiliki karakteristik nutrisi paling baik terutama protein sebesar 28,92%. Karakteristik fisik pakan terbaik melalui uji ketahanan pakan di air ditunjukkan oleh pakan pelet.Increment of aquaculture production cost caused by expensive feed containing high protein has made exploration of feed stuffs having high nutrition, low cost, and annually available continuously to be done. Investigation of alternative stuffs was expected to increase protein content of cheap commercial feed containing low protein. The methods of this research consisted of producing experimental feed, proximate analysis, and feed stability test in the water. The treatments were (A) combination of tofu waste 80% + feed 20%, (B) combination of tofu waste 60% + feed 40%, (C) combination of tofu waste 40% + feed 60%, (D) combination of tofu waste 20% + feed 80%, (E) control (feed 100%). This research resulted the best nutrition characteristic obtained from combination of tofu waste 40% + feed 60% in which its protein was 28,92%. The best physical characteristic through feed stability test in the water was obtained from commercial feed (control).

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