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Production of Clarias gariepinus seeds through accelerated female broodstock rematuration Prama Hartami; Mahdaliana Mahdaliana; Eva Ayuzar
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22860

Abstract

This study aims to determine the effectiveness of Oodev and Turmeric flour on feed to accelerate the rematuration of female broodstock catfish for increasing seed production. Oodev hormone is believed to accelerate the maturation process of broodstock, while turmeric flour with its active ingredients can improve liver performance in the vitellogenesis process. The method was a non-factorial completely randomized design with 4 treatments and 3 replications. The treatments are Control, (A) Oodev 0.3 ml/kg of feed, (B) Turmeric flour 5 gr/kg of feed, and (C) Combination of Oodev + Turmeric flour. The results showed that C treatment was the best treatment which produced a GSI value of 14.57 ±2.53%, period of rematuration was 32.33 ±4.16 days, egg diameter was 1.27 ±0.12 mm, fecundity was 22,939.97 ±880.38 eggs/kg of broodstock weight, fertilization rate was 80.18 ±9.75%, hatching rate was 79.80 ±2.88%, and larval survival reached 83.17 ±9.69%. Meanwhile, the specific growth of seed length in all treatments had no significant effect between treatments. The results of this study will certainly provide useful information for increasing catfish seed production both in Aceh and on a Nationally. This acceleration of maturation also has a positive impact in maximizing the available broodstock without having to increase the number of catfish broodstock to produce seeds of superior quality and benefit the cultivators.Keywords:EnrichmentFemale BroodstockPerformanceRematurationReproduction
Implementasi Pretreatment Ekstraksi Lipid untuk Produksi Biodiesel Nannochloropsis sp. yang Dikultivasi dalam Media Limbah Budidaya Vaname Rusydi, Rachmawati; Alfaini, Momika; Ayuzar, Eva; Hartami, Prama; Salamah, Salamah; Muliani, Muliani; Khalil, Munawwar; Febri, Suri Purnama
Jurnal Ilmiah Samudra Akuatika Vol 8 No 1 (2024): Jurnal Ilmiah Samudra Akuatika
Publisher : Fakultas Pertanian Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jisa.v8i1.10831

Abstract

Nannochloropsis sp. is the green colored microalgae with its round shape of cell. This microalgae has lipids of 28,7% by dry weight, especially unsaturated fatty acids which can be transesterified to produce methyl ester and be a potential as biodiesel feedstock. The content of biodiesel Nannochloropsis sp. reaches 80% of dry weight and has the sama characteristics as vegetable oil. In the lipid extraction process many problems were found, one of which was the cell wall of Nannochloropsis sp. which prevents the lipid from being extracted. This research was carried out on 10 December 2022-28 February 2023 at the Hatchery and Aquaculture Technology Laboratory aquaculture study program, faculty of agrigulture, Malikussaleh University, Chemical Technology Laboratory at Lhokseumawe State Polytechnic and at The Environmental Quality Testing Engineering Laboratory At Syiah Kuala University, Banda Aceh. This study used a laboratory experimental method using a Non-Factorial Completely Randomized Design (CRD) consisting of 4 treatments and 3 replications. Treatment A Control, treatment B Heating, treatment C Osmotic shock and treatment D Homogenizer. Based on statistical analysis of the F test (ANOVA) showed that the percentage of lipid from the application of pretreatment gave significantly different resulst to the percentage of lipid from the application of pretreatment. The highest lipid was found in treatment B which was 21,14% and the lowest lipid was in treatment C which was 12,51%. The highest lipid producer from the application of pretreatment is using the heating method. Biodiesel is obtained through a tranesterification process using methanol as a solvent and KOH catalyst.
UJI KETAHANAN PENYAKIT MOTILE AEROMONAS SEPTICEMIA (MAS) MENGGUNAKAN BAKTERI Aeromonas hydrophila PADA IKAN PATIN TRIPLOID Hartami, Prama
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 20, No 1 (2024): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.20.1.%p

Abstract

Motile Aeromonas Hydrophila (MAS) disease caused by Aeromonas hydrophila is a common pathogen that attacks freshwater fish commodities, including triploid as the new strain of striped catfish. The purpose of this study was to test the resistance of triploid catfish to MAS infection before being released to cultivators as a new commodity to increase national striped catfish production. The test was carried out for 2 months at the Sukamandi Fish Breeding Research Institute, Subang by injecting A. hydrophila bacteria into triploid and diploid catfish. The challenge test was carried out by injecting the cultured A. hydrophila bacteria with a density of 107 cfu.mL-1 as much as 0.1 mL.tail-1 at fish intramuscularly in all treatments, while in the Control (-) treatment, 0.1 mL of PBS solution was injected.tail-1. The test striped catfish used for each treatment were 10 tails measuring 9.23 – 9.65 cm, and weighing 7.1 – 9.23 g. Survival parameter data and blood picture were analyzed quantitatively using Microsoft Excel 2010 and SPSS version 16.0 software with analysis of variance (ANOVA) at 95% confidence interval. The results of re-characterization with biochemical tests showed that A. hydrophila bacteria were gram negative, oxidase and catalase positive, motile, could fermentative convert sugar and could grow in blood media. The survival of triploid striped catfish after the test was 95%, total erythrocytes on day 7, and total leukocytes on the first day were significantly different from diploid striped catfish. Thus, it can be concluded that striped triploid catfish are more resistant to infection with A. hydrophila bacteria than diploid.
Implementation of Pretreatment in Lipid Extraction to Biodiesel Production from Nannochloropsis sp Cultivated in Vannamei Farming Wastewater Rusydi, Rachmawati; Alfaini, Momika; Ayuzar, Eva; Hartami, Prama; Salamah, Salamah; Muliani, Muliani; Khalil, Munawwar; Febri, Suri Purnama
Jurnal Ilmiah Samudra Akuatika Vol 8 No 1 (2024): Jurnal Ilmiah Samudra Akuatika
Publisher : Fakultas Pertanian Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jisa.v8i1.10905

Abstract

Nannochloropsis sp. is the green colored microalgae with its round shape of cell. This microalgae has lipids of 28,7% by dry weight, especially unsaturated fatty acids which can be transesterified to produce methyl ester and be a potential as biodiesel feedstock. The content of biodiesel Nannochloropsis sp. reaches 80% of dry weight and has the sama characteristics as vegetable oil. In the lipid extraction process many problems were found, one of which was the cell wall of Nannochloropsis sp. which prevents the lipid from being extracted. This research was carried out on 10 December 2022-28 February 2023 at the Hatchery and Aquaculture Technology Laboratory aquaculture study program, faculty of agrigulture, Malikussaleh University, Chemical Technology Laboratory at Lhokseumawe State Polytechnic and at The Environmental Quality Testing Engineering Laboratory At Syiah Kuala University, Banda Aceh. This study used a laboratory experimental method using a Non-Factorial Completely Randomized Design (CRD) consisting of 4 treatments and 3 replications. Treatment A Control, treatment B Heating, treatment C Osmotic shock, and treatment D Homogenizer. Based on statistical analysis of the F test (ANOVA) showed that the percentage of lipid from the application of pretreatment gave significantly different resulst to the percentage of lipid from the application of pretreatment. The highest lipid was found in treatment B which was 21,14% and the lowest lipid was in treatment C which was 12,51%. The highest lipid producer from the application of pretreatment is using the heating method. Biodiesel is obtained through a tranesterification process using methanol as a solvent and KOH catalyst.
Pendampingan Kelompok Maju Bersama dalam Upaya Memproduksi Benih Ikan Lele Mutiara (Clarias sp) Hartami, Prama; Nirzalin, Nirzalin; Martina, Martina; Hatta, Muhammad; Rafi, Muhammad; Zuraida, Zuraida; Rizki, Muhammad
INTEGRITAS : Jurnal Pengabdian Vol 7 No 2 (2023): AGUSTUS - DESEMBER
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/integritas.v7i2.2285

Abstract

Kegiatan pembenihan merupakan upaya yang dilakukan untuk memproduksi benih agar tidak terjadi ketergantungan stok pada suplier benih, sehingga kegiatan pembesaran ikan lele tidak mengalami kendala produksi yang diakibatkan oleh keterbatasan benih atau mahalnya harga benih untuk ukuran tertentu. Hal inilah yang dilakukan oleh Tim Pengusul kepada Mitra yang tergabung dalam Kelompok Usaha Maju Bersama di Gampong Blang Kubu, Kecamatan Peudada, Kabupaten Bireuen. Tujuan pengebdian ini adalah untuk membantu kelompok mitra agar mampu menyediakan benih secara mandiri untuk kebutuhan pembudidayaan ikan lele bagi para anggota dan masyarakat sekitar yang tertarik untuk terlibat dalam usaha pembesaran ikan lele. Kegiatan Pengabdian ini dilaksanakan mulai tanggal 05 September hingga 05 November 2022 menggunakan metode deskriptif kualitatif pada kegiatan pembenihan ikan lele mutiara melalui induced spawning. Tahapan kegiatan pendampingan ini meliputi hal-hal seperti penyediaan induk unggul, persiapan wadah, perawatan induk, seleksi induk, dan proses pemijahan yang dilakukan. Hasil pendampingan khusus kepada mitra didapatkan bahwa banyak dukungan penuh yang diberikan kepada tim pelaksana agar target yang ingin dicapai bisa terlaksana secara optimal, hal ini terlihat dari banyak fasilitas yang dimiliki mitra dapat secara penuh dimanfaatkan oleh tim pengusul.