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All Journal Medical Journal of Indonesia Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Journal Orthopaedi and Traumatology Surabaya (JOINTS) Journal of Global Pharma Technology The Hip and Knee Journal
Ismail Hadisoebroto Dilogo
Department Of Orthopaedic And Traumatology, Faculty Of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Health Professions Medicine & Pharmacology Neuroscience

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Journal : Journal of Global Pharma Technology

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PASSAGE INFLUENCE ON THE AGING PROFILE AFTER SUCCESSIVE CRYOPRESERVATION OF BONE MARROW MESENCHYMAL STEM CELLS Ismail Hadisoebroto Dilogo; Starifulkani Arif; Jeanne Adiwinata Pawitan; Ria Anggraeni; Yogi Ismail Gani
Journal of Global Pharma Technology Volume. 9 Issue 6
Publisher : Journal of Global Pharma Technology

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Objective: Bone marrow mesenchymal stem cells are promising in cell therapy but the low frequency of this subpopulation necessitates their in vitro expansion and cryopreservation prior to clinical use. The aim of this study was to analyze the passage effect on the aging profile of previously successive cryopreserved bone marrow mesenchymal stem cells.  Methods: The samples of this in-vitro observational analytic study (April - September 2016) were iliac crest bone marrow mesenchymal stem cells that were cryopreserved in passage one and a second group was cryopreserved after being subcultured. It was obtained from UPT TK Sel Punca RSCM-FKUI. Samples were analyzed for each passage in terms of cell size, viability, population doubling time (PDT), and percentage of senescent cells by ANOVA test and independent t-test for the inter-cryopreservation group.Results: The remarkable manifestation of senescence appeared at passage six in both cryopreserved groups, but tend to appear earlier in the twice cryopreservation group. The senescent positive cell size became bigger along with the increasing number of passage. There were significant differences in PDT, 30% confluent cell size, viability, in passage six between the two cryopreservation groups (p<0.001, p<0.001, p=0.022), respectively, but no significant difference in percentage of senescent cells (p= 0.052). The cells became senescent in P6 in both groups (P=0.000).Conclusion: The onset of senescence of bone marrow mesenchymal stem cells was in passage six, while PDT, cell size and viability differ significantly due to successive cryopreservation.
The Effectivity of Porous Hydroxyapatite loaded with Gentamycin on Chronic Tibial Osteomyelitis in Rabbit Models Ismail Dilogo
Journal of Global Pharma Technology Volume 09 Issue 04
Publisher : Journal of Global Pharma Technology

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Introduction. Osteomyelitis management with debridement, guttering, and systemic antibiotics often do not give satisfactory results due to the inability of antibiotics to reach the infection site. Another method of antibiotic delivery in the form of beads are deemed ineffective as it requires two separate surgeries. Combination of local antibiotics with absorbable porous hydroxyappetite scaffolding  and antibiotic carriers have not been studied. Methods. We conducted pre-test and post-test control group in a rabbit model of osteomyelitis. Ten rabbits divided in control group and the treatment group (n=5). We injected Staphylococcus aureus in the rabbit tibia, forming the osteomyelitis model, and subsequently performed treatment for osteomyelitis. In the control group, we performed debridement and administered ceftriaxone injection for 4 weeks, whereas in the treatment group, we added the combination of porous hydroxyapatite and gentamicin. Afterwards, we performed clinical assessment, x-ray examination, culture, and histopathology.Results. Radiologically, tibia cortical thickening scores improved in the treatment group compared to the control group (p=0.48) as well as histopathological osteomyelitis evaluation score (p=0,009). Clinically, there were improvements in the swelling scores (n=5) of the treatment group compared to control group, but no significant statistically (p=0.053). In culture, there were no significant difference between the two groups (p=1.00).Conclusion. Combination of porous hydroxyapatite and gentamicin as a local treatment of osteomyelitis of the rabbit tibia osteomyelitis models improved radiological and histopathological scores and also clinically compared to existing standard treatment procedures for chronic osteomyelitis
Co-Authors Achmad A. Yusuf Achmad F. Kamal Achmad Fauzi Kamal Adang Bachtiar Ahmad A. Jusuf Ahmadin Yusuf Rizal Susatyo Andri M.T. Lubis Aprilya, Dina Aria Kekalih Bernadus Riyan Hartanto Canintika, Anissa Feby DIAH ISKANDRIATI Eko Prasetyo Adi Wibowo Endrotomo Sumargono Erica Kholinne Errol U. Hutagalung Farhan Haidar Fazlur Rahman Fiolin, Jessica Fitri Octaviana Giarso, Peter Harahap, Alida R. Herdiman, Joshua Alward Heri Suroto Immanuel P. Sitorus, Immanuel P. Immanuel Panca Sitorus Jeanne Adiwinata Pawitan Jessica Fiolin Jessica Fiolin Kousar, Iqra Ludwig A. Pontoh Mahmood, Amer Milton Lee Chip Routt Mohammad Hidayat Mohammad Zaim Chilmi Muhammad Budimansyah Nizar Al Rhaazi Nurjati C. Siregar Nurjati Chairani Siregar Oesman Ihsan Pandelaki, Jacub Phedy Phedy Puspita Eka Wuyung Putra, Anggaditya R. Susworo Radiana Dhewayani Antarianto Rahyussalim Rahyussalim, Rahyussalim Ria Anggraeni Saptawati Bardosono Sholahuddin Rhatomy Starifulkani Arif Wahyu Widodo Wahyu Widodo William Chandra Yogi Ismail Gani Zuhri Efendi