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All Journal Medical Journal of Indonesia Damianus Journal of Medicine Indonesian Journal of Obstetrics & Gynecology Science Majalah Patologi Indonesia Indonesian Journal of Cancer Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute
Nurjati Chairani Siregar
Fakultas Kedokteran Universitas Indonesia, Jakarta
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Biopsi cair pada kanker kolorektal: Harapan dan tantangan Angka, Rebecca Noerjani; Lumban Tobing, Demak; Sudoyo, Aru Wisaksono; Siregar, Nurjati Chairani; Jeo, Wifanto Saditya
Bahasa Indonesia Vol 21 No 3 (2022): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v21i3.3575

Abstract

Pendahuluan: Global Cancer Statistics 2020 menunjukkan angka kejadian kanker kolorektal menduduki peringkat ketiga dengan urutan kedua angka kematian. Pemeriksaan biopsi cair pada kanker kolorektal mulai dilakukan untuk mempelajari sel-sel tumor yang ada di dalam sirkulasi yaitu circulating tumor cells (CTCs) dan cell-free circulating tumor DNA (ctDNA/cfDNA). Tujuan dari penulisan artikel ini adalah untuk menemukan dan menelaah penelitian-penelitian yang telah dilakukan mengenai biopsi cair baik dari sisi metode, hasil maupun manfaat klinik pada kanker kolorektal. Metode: Metode pencarian literatur dilakukan melalui Embase, Scopus dan PubMed. Dari 143 artikel yang ditemukan saat pencarian awal, setelah melalui tahap seleksi akhir ditentukan 15 artikel yang memenuhi kriteria inklusi. Hasil: Biopsi cair dapat digunakan untuk mendeteksi mutasi yang tidak ditemukan pada tumor primer pasien kanker kolorektal serta analisis kombinasi circulating cell-free DNA (ccfDNA) dan ctcDNA dapat meningkatkan jumlah mutasi yang terdeteksi. Analisis ctDNA dalam plasma yang dikumpulkan secara serial, memungkinkan mendeteksi ctDNA mutan yang muncul, sehingga dapat digunakan untuk memantau perkembangan penyakit pasien serta menemukan mekanisme resistensi. Pengambilan sampel pasien secara serial menunjukkan bahwa penurunan metilasi terjadi pada pasien yang berespon baik terhadap pengobatan, baik operasi maupun kemoterapi. Sebaliknya pasien yang tidak diobati atau mengalami kekambuhan menunjukkan peningkatan metilasi. Status MSI pada kanker kolorektal yang terdeteksi melalui cfDNA relatif konsisten dengan yang ada di sampel jaringan tumor. Simpulan: Keuntungan biopsi cair adalah darah lebih mudah didapat, efektif dan tidak invasif dibandingkan dengan biopsi terutama pada tumor yang sulit dijangkau dengan biopsi. Biopsi cair dapat dilakukan berulang secara serial sehingga dapat digunakan untuk memantau perkembangan penyakit, mengetahui respon pengobatan dan menentukan prognosis.
Innate Immune Response in Non Alcoholic Fatty Liver Disease: An Overview of Alterations in TLR9, Macrophages, and TNFα Supriatna, Novianti; Siregar, Nurjati Chairani
Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education Research Institute Vol. 6 No. - (2022): Proceedings Book of International Conference and Exhibition on The Indonesian M
Publisher : Writing Center IMERI FMUI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69951/proceedingsbookoficeonimeri.v6i-.131

Abstract

Non-Alcoholic Fatty Liver Disease (NAFLD) and Alcoholic Liver Disease (ALD) are two causes of chronic liver disease. In Indonesia, NAFLD is the most common case of hepatic disease for Indonesian people. Risk factors such as obesity, diabetes mellitus, and metabolic syndrome increase the risk of developing NAFLD. About 1.5 billion people worldwide suffer from fatty liver, with a prevalence of 25-30%. Hence, it will continue to increase sharply if risk factors or diseases are not mitigated with pharmacological treatment and diet. The innate immune response induces abnormalities in fatty liver, mediated by Kupffer cells and TLR9 (Toll-Like Receptor 9), leading to inflammation via proinflammatory cytokines, like TNFα. Hepatocyte injury releases signals in the form of mitochondrial DNA enclosed in microparticles, which travels into the plasma, and are then captured by TLR9. Activation of TLR9 on Kupffer cells is the starting point of the inflammatory process in the pathogenesis of NAFLD, and it triggers other immune responses that encourage the development of steatohepatitis until a more severe liver injury occurs. The latest study found that analysis is needed to determine the immunological mechanisms and therapeutic targets associated with inflammation in fatty liver disease as evidenced by various immunological approaches.
Tumor Microenvironment in Colorectal Cancer Development: A Review of 3D Study Analysis Angka, Rebecca Noerjani; Sudoyo, Aru Wisaksono; Siregar, Nurjati Chairani; Jeo, Wifanto Saditya; Antarianto, Radiana Dhewayani
Indonesian Journal of Cancer Vol 18, No 1 (2024): March
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v18i1.1089

Abstract

Background: Colorectal cancer is the third most common cancer in 2020, with a high mortality rate. Colorectal cancer treatment has made many advances. However, many factors in the tumor microenvironment still have not been reached but significantly affect the success of treatment. This literature review was conducted to search for research articles that analyze factors in the tumor microenvironment, how they interact through studies primarily conducted in 3D, and how to prepare for 3D research on colorectal cancer will be briefly discussed. The aim of this systematic review is to study the components of the tumor microenvironment in colorectal cancer development as assessed by 3D studies. Methods: Article searches were conducted through Embase, Scopus and PubMed. From the 110 articles found at the beginning of the search, after going through several screening stages, 27 articles were determined that met the inclusion criteria. The inclusion criteria used were journals containing research articles on colorectal cancer in the last five years with topics regarding the tumor microenvironment and according to keywords. Results: Microenvironment components in colorectal cancer, consisting of cellular and noncellular components, have the most significant effect on cancer development and ultimately affect metastasis, response to treatment, and prognosis.  Conclusions: This literature review proves that the components of the tumor microenvironment are very diverse, making colorectal cancer heterogeneous. It still requires a lot of research to prove the existence of other components that affect the effectiveness of the treatment.
Adipose-derived mesenchymal stem cells enhance regeneration in a chronic peripheral sciatic nerve injury Sprague-Dawley rat model Widodo, Wahyu; Dilogo, Ismail Hadisoebroto; Kamal, Achmad Fauzi; Antarianto, Radiana Dhewayani; Wuyung, Puspita Eka; Siregar, Nurjati Chairani; Octaviana, Fitri; Kekalih, Aria; Suroto, Heri; Aprilya, Dina; Canintika, Anissa Feby
Medical Journal of Indonesia Vol. 34 No. 3 (2025): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.257780

Abstract

BACKGROUND Obtaining optimal functional outcomes in patients with chronic peripheral nerve injuries (PNIs) remains challenging due to the limited regeneration of the neuromuscular junction (NMJ). Adipose-derived mesenchymal stem cells (AD-MSCs) can differentiate into Schwann-like cells, secrete neurotrophic factors, and recruit native Schwann cells. This study aimed to analyze the effects of AD-MSCs on functional outcomes in a chronic PNI model. METHODS An in vivo study was performed using 20 male Sprague-Dawley rats with chronic PNI of the right sciatic nerve. Rats were divided into two groups: the AD-MSC group (n = 10), receiving human AD-MSC injections in the NMJ; and the control group (n = 10), receiving normal saline injections. Walking track analysis and electrophysiological assessments were performed 8 weeks after the nerve repair. Postmortem gastrocnemius muscle weights and immunohistochemical examinations were also performed. RESULTS The AD-MSCs showed significantly larger fiber diameters than the control group (45.54 [13.1] versus 35.46 [6.64]; p = 0.011). While clinical, electrophysiological, and gastrocnemius muscle weight data suggested a trend toward improved outcomes in the AD-MSCs group, the differences were not statistically significant. CONCLUSIONS AD-MSC implantation may enhance nerve regeneration, as demonstrated by a better outcome profile in the AD-MSCs group.
Estrogen receptor expression in type 1 endometrial cancer and its association with lymphovascular space invasion Fidiawati, Wiwit Ade; Andrijono; Siregar, Nurjati Chairani; Prihartono, Joedo; Purwoto, Gatot; Hellyanti, Tantri
Medical Journal of Indonesia Vol. 34 No. 4 (2025): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.257842

Abstract

BACKGROUND Endometrial cancer (EC) ranks sixth in female genital malignancy and originates in the endometrial lining. Estrogen receptor (ER) expression is important in EC prognosis and recurrence, influenced by the presence of lymphovascular space invasion (LVSI). This study aimed to determine the association between ER expression and LVSI in type 1 EC. METHODS A retrospective analysis was conducted on 135 patients with type 1 EC who underwent surgery at the Cipto Mangunkusumo Hospital, Jakarta, Indonesia, between January 2012 and December 2022. Immunohistochemistry (IHC) with ER antibodies was performed on all samples in April 2023. ER expression was evaluated using the Allred scoring system, and its association between ER expression (percentage and intensity stain) and LSVI was statistically analyzed. RESULTS Of 135 samples, 44 (32.6%) were LVSI-positive. No significant association was found between IHC percentage stain and LVSI (p = 0.994). However, a significant association was found between IHC stain intensity and LVSI-positive in patients with type 1 EC (p = 0.022). ER intensity score 2 had a higher LVSI risk compared to score 1 and score 3 (from 51%, 26%, and 26%, respectively). CONCLUSIONS ER expression is associated with LVSI of type 1 EC. While IHC stain percentage showed no correlation with LVSI, stain intensity was significantly linked to LVSI-positive in patients with type 1 EC. Regular assessment of ER expression can provide significant prognostic information, support hormonal therapy, and identifying clinical characteristics of tumors.
Non-Severe Preeclampsia and Subclinical Inflammation: A Study of Cyclophilin A, NF-κB, PARP- 1, and Apoptosis in Human Placentas Resistantie, Novi; Wibowo, Noroyono; Prasmusinto, Damar; Jusman, Sri Widia Azraki; Yamin, Muhammad; Siregar, Nurjati Chairani; Prihartono, Joedo; Mose, Johannes Cornelius; Suhendro, Suhendro; Yunita, Ferdiana; Rosmanah, Lis; Margyaningsih, Nur Ita; Qotrunnada, Labibah; Roviqoh, Cindy Fawwaz; Rauf, Saidah
Indonesian Journal of Obstetrics & Gynecology Science Volume 8 Nomor 3 November 2025
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia.v8i3.947

Abstract

Objective: To compare the expression of CyP-A, NF-κB, PARP-1, and apoptotic index in Non-Severe Preeclampsia (NS-PE) and Normal Pregnancy (NP) and explore their roles in inflammation during preeclampsia.Methods: Conducted in Depok, Indonesia, the cross-sectional study involved 28 participants divided into NS-PE and NP groups based on ISSHP criteria. NP was defined as uncomplicated pregnancies at 38–40 weeks gestation. Placental weight was measured, and ELISA was used to assess biomolecule levels. Data were analyzed using T-tests or Mann-Whitney tests.Result: Maternal gestational age, body mass index, and leukocyte levels were significantly higher in NS-PE. The apoptotic index, measured by TUNEL assay, was also significantly elevated in NS-PE (41.56 ±24.87) compared to NP (23.96 ±18.79; p = 0.044). While CyP-A, PARP-1, and NF-κB levels were higher in NS-PE eventhough they were not statistically significant. Immunohistochemistry confirmed an overall increase in these molecules, supporting their clinical relevance.Conclusion: Despite the lack of statistical significance, increased inflammation and apoptosis in NS-PE may contribute to placental dysfunction and adverse pregnancy outcomes.Non-Severe Preeclampsia dan Inflamasi Subklinis: Studi CyP-A, NF-κB, PARP-1, dan Apoptosis pada Plasenta ManusiaAbstrak Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan ekspresi CyP-A, NF-κB, PARP-1, dan indeks apoptosis antara preeklamsia non-severe (NS-PE) dan kehamilan normal (NP), serta perannya dalam proses inflamasi pada preeklamsia.Metode: Metode penelitian yang digunakan dalam penelitian ini adalah cross-sectional. Penelitian ini dilakukan di Depok, Indonesia, dengan 28 partisipan yang dikelompokkan menjadi NS-PE dan NP berdasarkan kriteria ISSHP. Berat plasenta diukur dan kadar biomolekul dianalisis menggunakan ELISA. Uji T dan alternatif Mann-Whitney digunakan untuk analisis statistik.Hasil: Hasil penelitian menunjukkan bahwa usia kehamilan, indeks massa tubuh (IMT), dan kadar leukosit secara signifikan lebih tinggi pada NS-PE. Indeks apoptosis (TUNEL) juga lebih tinggi secara signifikan pada NS-PE (41,56 ±24,87) dibandingkan NP (23,96 ±18,79; p = 0,044). Kadar CyP-A, PARP-1, dan NF-κB lebih tinggi pada NS-PE meskipun tidak signifikan secara statistik, pemeriksaan IHK mengonfirmasi relevansi klinis peningkatan pada keseluruhan biomolekul tersebut. Kesimpulan: Meskipun signifikansi statistik rendah, peningkatan peradangan dan apoptosis pada NS-PE dapat menyebabkan disfungsi plasenta dan dampak buruk pada kehamilan.Kata kunci: Apoptosis; inflamasi; preeklamsia.
Ekspresi New York Esophageal Squamous Cell Carcinoma-1 (NYESO-1) pada Sarkoma Sinovial dan Non Sarkoma Sinovial Lainnya Sebagai Dasar Pemilihan Pemberian Imunoterapi Ros Nirmawati,; Siregar, Nurjati Chairani; Evelina Kodrat
Majalah Patologi Indonesia Vol. 30 No. 3, September 2021
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v30i3.483

Abstract

BackgroundSynovial sarcoma is a rare high grade soft tissue sarcoma. Nowdays, the available therapeutic modalities has not given asatisfactory result yet. Currently, there is a promising therapeutic strategy through immunotherapy targeting NY-ESO-1 which isexpressed on tumor. The aim of this study was comparing NY-ESO-1 immunoexpression between synovial sarcoma and itshistologic mimics i.e. malignant peripheral nerve sheath tumor (MPNST) and dermatofibrosarcoma protuberans (DFSP)MethodsA cross sectional study was done in 28 cases of synovial sarcoma, 10 cases of MPNST and 17 cases of DFSP from archievalmaterial in Department Anatomical Pathology, FMUI/RSCM from January 2013 to June 2019. Immunohistohemical stainning wasperformed using an antibody NY-ESO-1 and it was described positive if it was expressed in more than 50% of tumor with moderateto strong positive intensity.ResultsThere is a significant difference (p<0.001) in NY-ESO-1 immunoexpression among synovial sarcoma (18/28), MPNST (2/10) andDFSP (1/17). Furthermore, synovial sarcoma showed a significantly higher immunoexpression compared to MPNST (OR 7.2;p=0.016; power 68.7%) and DFSP (OR 28.8; p<0.001; power 98.9%).ConclusionSynovial sarcoma showed a higher expression of NY-ESO-1 thus makes it as a good candidates for immunotherapy. There aredifferences in the expression of NY-ESO-1 in synovial sarcoma against MPNST and DFSP.
Optimal time point for evaluation in a chronic peripheral nerve injury rat model: a preclinical study Widodo, Wahyu; Dilogo, Ismail Hadisoebroto; Kamal, Achmad Fauzi; Antarianto, Radiana Dhewayani; Wuyung, Puspita Eka; Siregar, Nurjati Chairani; Octaviana, Fitri; Kekalih, Aria; Suroto, Heri; Aprilya, Dina; Canintika, Anissa Feby
Medical Journal of Indonesia Online First
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.257778

Abstract

BACKGROUND Peripheral nerve injury (PNI) can cause severe functional disabilities and progresses dynamically over time. Since evaluations held at different time points will yield different results, finding an optimal model for PNI comparison is needed. This study aimed to create an animal model of chronic denervation that simulates the progress of nerve injury. METHODS 6 male Sprague-Dawley rats underwent complete sciatic nerve transection in their right hind limbs, with severed nerve ends secured to a nearby muscle to prevent nerve regrowth. The rats were sacrificed at 2, 3, and 4 weeks. The assessment included walking analysis (pre- and post-injury), wet muscle weight measurement, and histological examination. RESULTS Progressive gastrocnemius muscle degeneration was observed at 3 different time points. Minimal degenerative changes were noted at 2 weeks, while extensive fibrosis (83.25 [12.19]% collagen area) appeared in the 4th week. The 3rd-week samples showed lymphocyte infiltrations, muscular atrophy, and progressive fibrosis, making it the best model for chronic PNI. CONCLUSIONS A 3-week chronic denervation model is proposed as a long-term PNI for further regenerative research.
Co-Authors Achmad Fauzi Kamal Andrijono Angka, Rebecca Noerjani Aprilya, Dina Aria Kekalih Canintika, Anissa Feby Damar Prasmusinto Evelina Kodrat Evelina Kodrat Fitri Octaviana Frans Suyatna Gatot Purwoto Hellyanti, Tantri Heri Suroto Ismail Hadisoebroto Dilogo Jeo, Wifanto Saditya Joedo Prihartono Jusman, Sri Widia Azraki kurnia agustini Lumban Tobing, Demak Margyaningsih, Nur Ita Mose, Johannes Cornelius Muhammad Yamin Noroyono Wibowo Puspita Eka Wuyung Qotrunnada, Labibah Radiana Dhewayani Antarianto Rauf, Saidah Resistantie, Novi Ros Nirmawati, Rosmanah, Lis Roviqoh, Cindy Fawwaz Sudoyo, Aru Wisaksono Suhendro Suhendro Supriatna, Novianti Wahono Sumaryono Wahyu Widodo Wiwit Ade Fidiawati Yunita, Ferdiana