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All Journal Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) JOURNAL ONLINE OF PHYSICS Martabe : Jurnal Pengabdian Kepada Masyarakat PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Indonesian Journal of Halal Research BEMAS: Jurnal Bermasyarakat International Journal of Engineering, Science and Information Technology Jurnal Kedokteran Mulawarman Kieraha Medical Journal Jurnal Pengabdian Masyarakat Bangsa
Adia Putra Wirman
Universitas Muhammadiyah Prof DR. Hamka, DKI Jakarta, Indonesia
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Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time Sunaryo, Hadi; Wirman, Adia Putra; Permanasari, Etin Diah; Nikmatullah, Nurul Azmah; Lestari, Dian; Nurjanah, Desi
Indonesian Journal of Halal Research Vol. 5 No. 2 (2023): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v5i2.21325

Abstract

DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins. Contamination of RNA, carbohydrates, lipids, and proteins can increase DNA purity. DNA purity was measured using a NanoDrop 2000 spectrophotometer measured by the absorbance ratio at 260 nm and 280 nm wavelengths. Good quality DNA will have an A260/A280 ratio of 1.7-2.0 and a concentration > 0.03 pg. This study aimed to obtain the appropriate DNA extraction method for fresh meat samples (a mixture of rat and chicken meat). This research consisted of two stages: the DNA extraction stage using the Progenus EasyFast™ Extraction Kit for Meat Products and the amplification stage using the EASYFAST™ Rat Detection Kit. This study used 16 samples of a mixture of rat meat and chicken with concentrations of rat meat: 5, 10, 15, and 20%. At the extraction stage, the incubation time was optimized for 15, 30, 45 minutes, and 1 hour. The results showed that the one hour incubation had a lowest CT value in the results of PCR amplification.
Edukasi Produk Kesehatan Halal Bagi Warga di Lingkungan Malaka Sari Jakarta Timur Hariyanti, Hariyanti; Nursal, Fith Khaira; Amalia, Anisa; Gantini, Sri Nevi; Wirman, Adia Putra
Jurnal Pengabdian Masyarakat Bangsa Vol. 3 No. 6 (2025): Agustus
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v3i6.2811

Abstract

Perkembangan produk halal dewasa ini sangat meningkat seiring dengan semakin tingginya kepedulian Masyarakat, khususnya muslim terhadap pola hidup sehari-hari. Undang-undang tentang Jaminan Produk Halal (JPH) yang mendorong munculnya lembaga-lembaga independent atau yang ditunjuk pemerintah dalam memfasilitasi pengakuan kehalalan produk tersebut. Produk kesehatan halal adalah produk yang dalam pembuatan serta pengemasannya mengikuti proses halal sesuai prosedur yang ditetapkan pemerintah melalui Sistem Jaminan Produk Halal (SJPH). Pengenalan produk obat dan kosmetika halal bertujuan untuk mengedukasi masyarakat dalam menggunakan produk yang digunakan sehari-hari dan menjadi kebutuha utama setelah makanan yang berdampak pada kesehatan tubuh. Penting bagi masyarakat mengetahui kehalalan setiap produk yang mereka gunakan dan menghindari rasa khawatir dari terpaparnya barang syubhat dan najis/haram. Edukasi diberikan dengan cara tatap muka langsung dan menyampaikan informasi lewat brosur serta media sosial, serta dilakukan penilaian sebelum dan setelah peserta mendapatakan edukasi. Berdasarkan hasil yang diamati selama kegiatan berlangsung, terdapat peningkatan pemahaman peserta atau warga terkait informasi dan cara memilih obat dan kosmetika halal, sehingga meningkatkan keamanan dan rasa nyaman bagi warga.
ANTIOXIDANT ACTIVITY AND DETERMINATION OF TOTAL TANNIN CONTENT OF 70% ETHANOL EXTRACT OF PLETKAN LEAVES (Ruellia tuberosa L. Wirman, Adia Putra
Jurnal Kedokteran Mulawarman Vol 12, No 2 (2025): Jurnal Kedokteran Mulawarman
Publisher : Fakultas Kedokteran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v12i2.16830

Abstract

Pletekan leaves are found in many regions in Indonesia, traditionally used for the treatment of hypertension, diabetes and antioxidants. Pletekan plants contain tannin compounds with phenol groups which are known to have biological effects such as antioxidants through mechanisms as free radical traps and electron donors. The aim of the research was to determine antioxidant activity using the ABTS (2,2-azinobis 3- ethyl benzothiazoline-6-sulfonic acid) method and testing total tannin content using the spectrophotometric method and Folin-Ciocalteu reagent and tannic acid as standards. Based on the research results, the 70% ethanol extract of Pletekan leaves has antioxidant activity as a free radical reducer which is in the medium category with an IC50 value of 215.07 ± 11.65µg/ml while the total tannin content is 114.60 ± 4.1 mg TAE/g.
Co-Authors Aggasy, Savira Yustinah Alfian Anisa Amalia DIAN LESTARI Etin Diah Permanasari Euis Purbasari Faridlatul Hasanah Fatimah Nisma Fith Khaira Nursal Gantini, Sri Nevi Hadi Nasbey Hadi Sunaryo Halida Sophia Hariyanti Hariyanti Hurip Budi Riyanti Kori Yati Kristiana, Helda Ladeska, Vera Lucy Efrieni Meri Suzana Meri Suzana Nikmatullah, Nurul Azmah Nurjanah, Desi Nurul Azmah Nikmatullah Oktadio Erikardo Purbasari, Euis Rahmadini, Nuriza Rahmi Wirman Wijiastuti Wijiastuti Yeni Yeni Yunazar Manjang