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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Indonesian Journal of Biotechnology The Indonesian Biomedical Journal 3BIO: Journal of Biological Science, Technology and Management Narra J Makara Journal of Science
Meitha, Karlia
School Of Life Sciences And Technology, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Survey on Ethnobotanic Value of Banana (Musa spp; Musaceae) in Bali Province, Indonesia Topik Hidayat; Himalaya Wana Kelana; Dhiyassalam Imam Anshori Ismanto; Karlia Meitha
HAYATI Journal of Biosciences Vol. 25 No. 1 (2018): January 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.945 KB) | DOI: 10.4308/hjb.25.1.31

Abstract

Bali, one of Indonesia island, is a region inhabited by a large number and varied banana (Musa spp; Musaceae). Many varieties of banana have been utilized by local peoples since long time ago as traditional medicine, edible material, used in traditional ceremony and others. However, information regarding the knowledge on ethnobotany of banana in Bali remains scattered and is not documented well. The purpose of this study was to evaluate and document the ethnobotanic values of bananas in Bali. Ethnobotanic data was collected through focus group discussion (FGD), surveys and interviews from 9 study sites (1 city and 8 regencies) with one or two villages represented each site. Ethnobotanical value of banana was determined by Local User’s Value Index (LUVI) with Pebble Distribution Method (PDM). Subsequently, data obtained was analysed using simple statistic description. Results showed that as many as 44 varieties of banana in Bali were documented. Local peoples have been utilizing banana in their daily life for ritual as indicated by higher LUVI (0.4867), followed by food (0.3), medicine (0.1533), and other (0.06). On the basis of testimony of respondents, indigenous knowledge of peoples in Bali about banana is vertically transmitted from parents to their children (98%). This study provided a valuable information of how the local peoples manage and conserve the banana and its nature.
Biodiversity of Bali Banana (Musaceae) and its Usefulness I Nyoman Rai; Fenny M. Dwivany; Agus Sutanto; Karlia Meitha; I Made Sukewijaya; I Nyoman Gede Ustriyana
HAYATI Journal of Biosciences Vol. 25 No. 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.098 KB) | DOI: 10.4308/hjb.25.2.47

Abstract

Banana (Musa spp.) is one the most important agriculture commodities in Indonesia. Archeological and molecular evidences suggest that speciation of this herb occurred in Indonesia, leading to the high diversity in the archipelago. In Bali Island, banana is not only sought for food but as well as for their symbolic role in religious and cultural ceremonies. However, the high demand for bananas in Bali is not yet supported by the adequate production of local farms. This presented study aimed to investigate the diversity of banana cultivars or sub-species in Bali and its usefulness to determine preferable cultivars to cultivate. We recorded and characterized 43 banana cultivars in 10 villages that represent the 8 regencies and 1 city of Bali province. Out of the 43 cultivars, 7 were highly used and at least one cultivar was discovered in each of the studied village.  The presence of these cultivars in the study areas were confirmed by site visit and characterization of the fruits. Among the highly ranked cultivars or species, only biu kayu is unique to Bali as it was not found in the closest provinces of East Java and Madura. Hence, the results suggested that to improve the cultivation and production of these 7 highly used cultivars could be an appropriate solution to meet Bali demand of bananas. Furthermore, cultivating biu kayu would also help conservation effort since this cultivar is also currently listed as a rare genetic resource.
Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region Karlia Meitha; Intan Fatmawati; Fenny Martha Dwivany; Agus Sutanto; Sigit Nur Pratama; Husna Nugrahapraja; Ketut Wikantika
Indonesian Journal of Biotechnology Vol 25, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.49506

Abstract

Pisang Kepok (Musa spp. [ABB ’Saba’ subgroup]) has several unique characteristics, such as tolerance to drought and Fusarium Foc (TR4) disease. Currently, the genetic diversity of Pisang Kepok in Indonesia is not well identified, although it is widely cultivated. Information on genetic diversity is essential for developing breeding strategies to achieve efficient cultivar improvement in the future. Aims of this research were to analyze the genetic variation of Pisang Kepok from some islands in Indonesia and to determine the genetic relationship between Pisang Kepok and other accessions banana cultivars based on ITS2 region, as a basis for future research in improving banana quality through molecular breeding. We have conducted the multiple sequence alignment and built the phylogenetic tree analysis using the Bayesian Inference Phylogeny method of one million generations (ngen = 1,000,000). The ITS2 region showed two clade ingroups: first clade consists of banana with B genome (balbisiana), while the second clade consists of banana with only A genome (acuminata). In general, all accessions of Pisang Kepok cultivars were clustered in the B genome of bananas cultivars. In addition, the ITS2 sequences and secondary structures among Pisang Kepok from various regions are identical, suggesting that there was no genetic variation in the ITS2 region of Pisang Kepok from multiple areas in Indonesia.
Chitosan suppresses the expression level of WRKY17 on red chili (Capsicum annuum) plant under drought stress Muhammad Abdul Aziz; Rizkita Rachmi Esyanti; Karlia Meitha; Fenny Martha Dwivany; Hany Husnul Chotimah
Indonesian Journal of Biotechnology Vol 25, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.55016

Abstract

Chili pepper plays a significant role in the global market. However, the production is often impeded by drought stress involving WRKY genes as the defense regulator. Chitosan is considered as a promising alternative fertilizer and defense elicitor. Hence, this study aimed to determine the role of chitosan in improving plant growth and survival of red chili pepper against drought stress. At the onset of the generative phase, chili plants were subjected to 1 mg mL‐1 chitosan, 50 percent drought, or chitosan‐drought treatment. Observations were made on several growth parameters, opened stomata, and WRKY gene expression. The results showed that chitosan‐drought treatment decreased plant growth and yielded significantly. The percentage of opened stomata was recorded at 0.56‐fold lower than control. It was followed by the decrease of the relative expression of WRKY17 and WRKY53 genes up to 0.56 and 0.72‐fold lower than control, respectively. Therefore, we suggested that the double treatment of chitosan‐drought might decrease plant growth performance but increase the defense system by suppressing the expression level of the WRKY17 gene. Interestingly, the drought treatment significantly increased WRKY17 expression level up to 7‐fold higher than control. Hence, it was suggested that WRKY17 has a specific role in response to drought stress.
In silico characterization and comparison of the fruit ripening related beta‐ amylase (BAM) gene family in banana genome A and B Erdianty Setiabudi; Karlia Meitha; Fenny Martha Dwivany
Indonesian Journal of Biotechnology Vol 26, No 4 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.65142

Abstract

Banana is one of the most important commodities for maintaining global food security. Primary metabolic processes during the ripening of banana greatly affect post‐harvest quality, particularly in starch metabolism. The beta‐ amylase (BAM) gene family is known as a group of genes that plays an important role in starch metabolism regulation. In this study, we focused on the characterization and comparative analysis of the BAM gene family in DH Pahang and Pisang Klutuk Wulung (PKW) varieties, these being the AA and BB genomes, respectively. The sequences of BAM gene family were retrieved from the database of Musa acuminata ’DH Pahang’ and Musa balbisiana ’PKW’ genome, then structural and functional characterization was performed, followed by identification of cis‐acting elements in the BAM promoter regions. The results showed that the BAM gene family structure was relatively conserved in both genomes, and a putative BAM11 gene was found, the function of which has not been studied in other plants. Cis‐acting element analysis showed that they were distinct in the copy number and types of elements that were responsive to various phytohormones. This study suggested that the BAM genes involved in ripening are spatiotemporally regulated. However, further functional genomic analysis is required to describe the specific role and regulation of BAM genes during ripening in banana.
Wheatgrass microgreen with high antioxidants content in an urban indoor farming system Myrea Chalil; Karlia Meitha; Ramadhani Eka Putra; Fathia Aulia Rahmah; Ridho R Sinatra; Anindha Ajeng Putri Winanta
3BIO: Journal of Biological Science, Technology and Management Vol. 4 No. 1 (2022)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2022.4.1.4

Abstract

Urban lifestyle is identical to stressful life and sedentary habit, leading to the increase of chronic conditions such as diabetes and cardiovascular-related diseases. Antioxidants are renowned for maintaining cellular function by quenching radicals produced in stressful conditions or infection. Fresh fruits and vegetables are the primary sources of antioxidants, but the long postharvest and transport system may reduce the benefits for the urban population. Hence, we designed a cultivation method to produce wheatgrass microgreens with high antioxidants in an urban indoor farming system. Generally, plants require light at the wavelength of 663 and 642 nm (red) and 430 nm and 453 nm (blue) to allow photosynthesis and production of secondary metabolites, such as antioxidants. We applied the LED lights with an RGB ratio of 91R/9B, 83R/17B, 47R/53B, 35R/65B, and white florescent as the control. Our results showed that 91R/9B reduced fresh mass and chlorophyll content, which might be due to the suppression of photosynthesis capacity. Interestingly, we found a significant (p<0.05) increase in carotenoids and flavonoid contents due to light combinations of 35R/65B and 83R/17B, respectively. However, the total antioxidants capacity was similar among all treatments. Carotenoids and flavonoids are among the antioxidants with a significant role in decreasing the risks of chronic diseases and their potential as antiviral agents. This cultivation system of wheat microgreen could be a promising solution to routinely supply carotenoids and flavonoids to the urban population. Further, it is also considered more environmentally friendly as it could be performed in a limited amount of land (vertically) and potentially use less energy for distribution.
In Silico Characterization of Lycopene Beta Cyclase (LCYB) and Lycopene Epsilon Cyclase (LCYE) Genes from DH-Pahang (Musa acuminata, A Genome) and DH-PKW (Musa balbisiana, B Genome): In Silico Characterization of LCYB and LCYE Genes I Wiprayoga; Karlia Meitha; Fenny Dwivany
Journal of Tropical Life Science Vol. 13 No. 1 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.01.09

Abstract

Alpha (α) and beta (β)-carotene are among the nutritious contents of bananas, with the unique feature of a high ratio of α-/β-carotene. Research on the gene and protein of the key enzymes determining the ratio of α-/β-carotene in bananas, namely lycopene beta cyclase (LCYB) and lycopene epsilon cyclase (LCYE), is currently not well defined. Hence, this study aimed to compare the characters of the LCYB and LCYE genes and their putative proteins from Musa acuminata 'DH-Pahang' and Musa balbisiana 'DH-PKW'. The corresponding nucleotide sequences from both species were aligned to detect similarities in the gene structure. Their protein products were characterized at the primary and tertiary levels. The phylogenetic tree was constructed based on nucleotide and protein sequences. The result showed that the gene structure between these two species is similar in LCYB in chromosome 9 but different in LCYB in chromosome 7 and LCYE. The presence of cis-acting regulatory elements in response to light dominated the 2000 nucleotide region of the 5'UTR of LCYB and LCYE genes in both species. Based on protein alignment and domain analysis, the NADB_Rossmann superfamily domain was detected in both LCYB and LCYE. Alignment of the three-dimensional protein structure showed a significant difference between MaLCYB.c07 and MbLCYB.c07 only. The phylogenetic tree based on protein sequences indicated the distant relationship of MaLCYB.c07 and MbLCYB.c07 with other LCYB ingroup OTUs. The results of this study could provide a molecular basis related to the exploration of bananas as a promising functional food to meet the needs of provitamin A.
sgRNA design and in vitro nucleolytic analysis of the Cas9‐RNP complex for transgene‐free genome editing of the eIF4E1 gene from Capsicum an‐ nuum L. Josefanny Tham; Alfred Patisenah; Tommy Octavianus Soetrisno Tjia; Santiago Signorelli; Intan Taufik; Karlia Meitha
Indonesian Journal of Biotechnology Vol 28, No 4 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.86778

Abstract

Chili (Capsicum annuum L.) is a highly valued vegetable, renowned for its unique taste and aroma. However, chili production faces challenges in meeting the high demand due to infections caused by pathogens such as ChiVMV (potyvirus). Previous studies have suggested that chili eIF4E1 plays a crucial role in potyvirus gene transcription. Therefore, this study explores the potential of CRISPR‐Cas9‐based genome editing to enhance chili resistance by introducing premature stop codons or truncated proteins. Two sgRNAs were designed, targeting the first and second intron of the eIF4E1 gene. The production of Cas9 protein was assessed with varying IPTG concentrations in Escherichia coli BL21(DE3), carrying 4xNLS‐pMJ915v2‐sfGFP plasmid with a TEV protease cut‐site at the N terminal. The findings indicate that the optimal IPTG concentration is 500 µM. Purification using an IMAC column confirmed the presence of Cas9 in the initial 2 mL of the eluted fractions, as indicated by numerous background proteins. Nevertheless, successful formation of Cas9‐RNP complexes was achieved for both sgRNAs. The nucleolytic activity of Tag‐Cas9 (carrying the MBP‐tag) and Cas9 was confirmed through in vitro endonuclease activity assays. The next step involved transfecting chili protoplasts with these RNP complexes to edit the chili eIF4E1 gene.
The In Silico Characterization of Lycopene Forming Phytoene Desaturase (CrtI) Protein from Wheat Leaf Rust Fungi (Puccinia triticina) Yehezkiel Vieri Polandos; Fenny Martha Dwivany; Karlia Meitha
3BIO: Journal of Biological Science, Technology and Management Vol. 5 No. 2 (2023)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2023.5.2.1

Abstract

Carotenoid is a highly economical compound with a variety of bioactivities. However, 98% of total carotenoid used is still being manufactured by chemical-based synthesis, reducing bioactivities and is not environmentally friendly, hence the use of biofortification approach is sought.Lycopene forming phytoene desaturase (CrtI) is one of the key enzymes with the potential to develop as bioparts in recombinant carotenoid biosynthesis. CrtI from Puccinia triticina and Blakeslea trispora are considered as promising candidates due to the high amount of carotenoid inthe fungi. This research aims to characterize CrtI enzyme from P. triticina and B. trispora and the interaction with substrate, i.e.,15 cis-phytoene. The results showed that CrtI from P. triticina protein has 2 unique motifs, determining the three-dimensional CrtI protein structure. According to docking analysis, CrtI enzyme from P. triticina is predicted to bind to the substrate more spontaneously as indicated by the lower energy of affinity (-8.3 kcal mol-1) and more residues interaction compared to CrtI from Blakeslea trispora. In conclusion, the CrtI protein from P. triticina is suggested as the candidate for further exploration to design expression in a recombinant system.
Development of an inactivated viral transport medium for diagnostic testing in low-resource countries Rahmani, Silmi; Meitha, Karlia; Septiani, Popi; Priharto, Neil; Kamarisima, Kamarisima; Ningrum, Ratih A.; Angelina, Marissa; Agustiyanti, Dian F.; Wisnuwardhani, Popi H.; Nugroho, Herjuno A.; Tan, Marselina I.
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2068

Abstract

Viral transport medium (VTM) is crucial for retaining clinical specimens, such as the virus or its genetic material from the mucus of respiratory tract of coronavirus disease 2019 (COVID-19) suspected patients. However, the locally produced VTM in Indonesia lacks the ability to inactivate the virus, risking the safety of diagnostic personnel. The aim of this study was to formulate inactive VTM (iVTM) incorporating chaotropic agents like guanidine salt, along with anionic detergents, chelators, buffers, and surfactants, to inactivate the virus while maintaining RNA integrity. Viral RNA stability in iVTM (pH 4 and pH 6) was evaluated for 30 days at 4°C and 25–28°C. In vitro inactivation test was performed on SARS-CoV-2 isolate (variant B1). The stability test revealed that storing the clinical specimens in iVTM at pH 6 maintained severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) detectability by qPCR for up to 30 days at cold and room temperatures. Stability assessments conducted over a 4-month period (at 25–28°C) on iVTM with a pH of 6 revealed clear appearance, consistent pH stability, no alteration in the solution color, and no indications of bacterial or fungal contamination. Results from an in vitro inactivation assay demonstrated that iVTM pH 6 eliminated SARS-CoV-2 infectivity within just five minutes of contact. These findings suggest that iVTM pH 6 offers a safer and cost-effective alternative for handling and transportation of clinical specimens.
Co-Authors Agus Sutanto Agus Sutanto Agustiyanti, Dian F. Alfred Patisenah Anindha Ajeng Putri Winanta Anjar Tri Wibowo Awik Puji Dyah Nurhayati BUDI SETIADI DARYONO Christianto, Antonius Dhiyassalam Imam Anshori Ismanto Dian F. Agustiyanti Dirgahyu, Anugrah Prima Erdianty Setiabudi Fathia Aulia Rahmah Fenny Dwivany Fenny M. Dwivany FENNY MARTHA DWIVANY Gunsi, Via Susana Hany Husnul Chotimah Herjuno A. Nugroho Husna Nugrahapraja I Made Sukewijaya I Nyoman Gede Ustriyana I NYOMAN RAI I Wiprayoga Intan Fatmawati Josefanny Tham Kamarisima Kamarisima Kamarisima, Kamarisima Kelana, Himalaya Wana Ketut Wikantika Latifah, Vida Rahma Marissa Angelina Marissa Angelina Marselina I. Tan Miftahudin . Muhammad Abdul Aziz Myrea Chalil Neil Priharto Neil Priharto Ningrum, Ratih A. Nugroho, Giri Nugroho, Herjuno A. Popi H. Wisnuwardhani Popi Septiani Putro, Karso Suryo RAHMANI, SILMI Ramadhani Eka Putra Ratih A. Ningrum Ridho R Sinatra Rizkita Rachmi Esyanti Santiago Signorelli Sartika, Dian Septiani, Popi Sigit Nur Pratama Silmi Rahmani Tan, Marselina I. Tan, Marselina Irasonia TATIK CHIKMAWATI Taufik, Intan Tommy Octavianus Soetrisno Tjia Topik Hidayat Wisnuwardhani, Popi H. Yehezkiel Vieri Polandos Yusuf, Adib Fakhruddin