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All Journal Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Tropical and Infectious Disease Pro-Life JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education Indonesia Journal of Halal Journal of Health Science and Prevention Cakrawala: Jurnal Litbang Kebijakan Indonesian Journal of Halal Research JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Journal of halal product and research (JHPR) BIO-EDU: Jurnal Pendidikan Biologi Jurnal Ilmiah Membangun Desa dan Pertanian BIOTROPIC
Saiku Rokhim
Universitas Islam Negeri Sunan Ampel Surabaya
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Journal : BIOTROPIC

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Keanekaragaman dan Kemerataan Jenis Collembola Gua di Kawasan Karst Malang Selatan widya pertiwi; Saiful Bahri; Saiku Rokhim; Nirmala Fitria Firdhausi
Biotropic : The Journal of Tropical Biology Vol. 4 No. 2 (2020): Biotropic, Volume 4 Nomor 2, 2020
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2020.4.2.134-139

Abstract

Karst adalah bentang alam yang unik yang terbentuk akibat dari pelarutan batuan karbonat atau batuan gamping oleh air hujan. Di dalam kawasan karst terbentuk beberapa gua yang menjadi ci khas dari kawasan karst yang terbentuk. Penelitian ini bertujuan untuk mengetahui keanekaragaman Collembola gua yang berada di Kawasan Karst Malang Selatan. Sampel yang diambil adalah berasal dari tanah yang berada di lantai gua dengan menggunakan teknik Purposiv Sampling. Penelitian dilakukan pada empat gua yaitu Gua Harta, Gua Krompyang, Gua Prapatan JLS, dan Gua Lowo. Setiap pengambilan sampel dilakukan tiga kali pengulangan pada setiap zona, yaitu zona terang, zona remang, dan zona gelap. Hasil analisis data menghasilkan nilai Indeks Keanekaragaman H’=0,46121 dan Indeks Kemerataan E=0,28656.
Optimization of RNA Extraction from Aedes aegypti and Aedes albopictus Saiku Rokhim; Ninik Fadhillah; Radinal Kautsar; Humayra Qurrata Aini; Yuanita Rachmawati
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1781

Abstract

RNA extraction is the critical initial stage in analyzing certain gene expressions, further analysis using Real Time PCR technology, and performing virus detection. However, the process of extracting RNA is often hampered by the risk of contamination, resulting in low concentrations of RNA and low purity of RNA. This is often an obstacle in extracting mosquito RNA especially detecting Dengue Virus (Den-V). Dengue virus (Den-V) can cause dangerous diseases in humans such as Dengue Fever (DHF) which is transmitted through the bites of Aedes aegypti and Aedes albopictus mosquitoes. This study aims to find out the effective steps for extracting RNA from Aedes aegypti and Aedes albopictus mosquitoes. The method being compared is a commercial RNA extraction kit with modification (addition of β-mercaptoethanol) and without modification. The results showed that the best DNA concentration and purity were obtained in mosquito samples from modified process. The purity ratio of RNA extracted without modification was 1.971 (0.021 ± 0.800) while with modification it was 2.003 (0.011 ± 0.112). Aedes aegypti had a better average concentration of 7.146 µg/ml for unmodified RNA and 7.613 µg/ml for modified RNA. This research is expected to be a reference for further studies on viruses in Aedes aegypti and Aedes albopictus.
Co-Authors Adzakiyyi, Miftahul Lathif AGUSTINA, EVA DAMAYANTI, MAMIK Dian Novitasari Erna Zahro’in Esti Tyastirin Faizah, Hanik Firmansyah, M. Aliffiyan Hakim, Ahmad Khairul Handayani, Ade Tiyan Hartono, Hermalya Putri Hawwa' Cahya Maulida Hermanto, M. Humayra Qurrata Aini Inggrit Tyautari Inggrit Tyautari Ita Ainun Jariyah Kautsar, Radinal Latifatoel Chilmi M. Aliffiyan Firmansyah Mauludin, Muchammad Firman Miftahul Lathif Adzakiyyi Misbakhul Munir Ninik Fadhillah Nirmala Fitria Firdhausi Nur Qomariyah Pratiwi Radinal Kautsar Ramadhani, Aisyah Hadi RIA SAFITRI Saiful Bahri Sari, Novita Mulya Siti Zulaikha Teguh Hari Sucipto, Teguh Hari Tri Susilowati Tri Susilowati Tri Susilowati Tyautari, Inggrit widya pertiwi Yosi Setia Prasiska Yuanita Rachmawati