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All Journal Universa Medicina Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner Journal Of Widya Medika Junior Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Widya Medika
Sianty Dewi
Widya Mandala Surabaya Catholic University
Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Optimized RT-qPCR Detection of Hepatic Lipopolysaccharide-Binding Protein in Diet-Induced Obese Mice Tjahjono, Yudy; Wedharga, I Gede Putu Adhi; Novita, Bernadette Dian; Tahalele, Paul; Wijaya, Hendy; Soediono, Endang Isbandiati; Hendrata, Adi Pramono; Dewi, Sianty; Wijaya, Sumi; Ervina, Martha; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.308-317

Abstract

High-throughput RT-qPCR results on hepatic lipopolysaccharide-binding protein (LBP) expression in obese subjects are essential, as they reveal the endotoxin’s role in the development of obesity and non-communicable disease (NCD). This study aimed to optimize RT-qPCR detection of LBP in diet-induced obese mice. This study primarily focused on addressing high variability through reference gene normalization. A total of six male C57BL/6 mice aged 6 weeks were randomly allocated into two dietary treatments (n = 3), consisting of mice fed with the standard chow diet (SCD group) and mice fed with the high-fat and high sucrose diet (HFHS group) ad libitum for 8 weeks. Relative quantification strategies involving the standard 2-ΔΔCt method (calibrator as mean) and the modified 2-ΔΔCt method (calibrator as individual sample-matched biological replicates) were compared in terms of their variability. Obesity was successfully induced in the HFHS treatment group, as indicated by significantly higher body weight, calorie intake, and LBP relative expressions compared to the SCD group. In addition, a sample-specific calibrator approach using the modified 2-ΔΔCt method resulted in lower variability in relative gene expression levels. A modified 2-ΔΔCt method, which utilizes a sample-specific calibrator to counteract sample-specific variability, was successfully employed to address high variability in RT-qPCR results.
HYPOGLYCEMIC, ANTI-INFLAMMATORY EFFECT OF PORANG (AMORPHOPHALLUS ONCHOPYLLUS) ON ALLOXAN-INDUCED DIABETIC RATS Young, Andre; Novita, Bernadette Dian; Ricardo, Evan; Dewi, I.G.A.P.D; Amadeo, Jovan; Dewi, Sianty; Tahalele, Paul
JURNAL WIDYA MEDIKA Vol. 9 No. 1 (2023): April
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v9i1.4173

Abstract

Introduction: Iles-iles / Porang is a tuber-producing plant that is commonly found in Indonesia. One of the most sought after content from Porang is Glucomannan. One of Porang’s health benefit is related to the effect of lowering blood glucose levels because it can prevent glucose absorption. These benefit is interesting to study because there has been no research linking the use of Porang to reduce inflammatory process in hyperglycmic conditions. Purpose: This study aims to analyze the anti-inflammatory, anti-oxidant and hypoglycemis effects of Glucomannan from Porang (Amorphophallus onchophyllus) extract in experimental animals. Method: The research design was a true experimental post-test only control group with random sampling to determine 5 white rats into the normal group, positive control group, negative control group and treatment 1, 2 and 3. The positive control group received Acarbose therapy thile the negative control group received carboxy-methyl-cellulose (CMC) therapy. This study used Porang extract (Amorphophallus onchophyllus) with concentrations of 200, 400 and 800 mg/Kg in hyperglycemic white rats that had been induced by Aloxan. The study was conducte for 50 days and then blood and serum samples were taken to assess the effect of hypoglycemia, anti-inflammatory and anti-oxidant using blood glucose, Malondialdehyde and C-reactiveprotein (CRP) measurement. Result and Discussion: The results showed no significant difference between the groups of rats receiving Porang extract and the positive and negative control groups. However, the MDA levels after 50 days of intervention between the negative control group and the therapy with doses of 200, 400 and 800 mg/Kg showed significant differences. Similarly found for blood glucose levels after intervention between negative control group and the 200 and 400 mg/Kg therapy group. This results may be caused by the type of Porang used, the form of the Porang and the concentration level of the Porang extract. Conclusion: Porang with the type of Amorphophallus onchophyllus can’t be used directly, but requires further processing to obtain the active substance Glucomannan.
Co-Authors Agatha Christi Palupi Amadeo, Jovan Amalia, Nindya Bernadette Dian Novita Bernadette Dian Novita Dewi, I.G.A.P.D Dian Putri, Maria Patricia Dyana Sarvasti Evelyn Ongkodjojo Ferry, Ferry Ferry, Ferry Hendrata, Adi Pramono Hertanto, Maria Patricia Dian Icha, Icha Imelda Theodora Immanuel Michael Jong, FX Himawan Haryanto Khudrati, Wilson Christianto Kuncorojakti, Suryo Lucia Hendriati Malonda, Franklin Vincentius Maria Amelia Suhardi Martha Ervina Meredith, Jennifer Ni Putu Sudewi, Ni Putu Novita, Bernadette Dian Paul L Tahalele Putra, Brilliant Dwi Ricardo Gunadi Ricardo, Evan Sandhy Prayudhana Soediono, Endang Isbandiati Soewangsa, Queency Amelia Sumi WIjaya, Sumi Teguh Widodo Tjahjono, Yudy Toynbee, Eddy Vincentius Diamantino Supit Wedharga, I Gede Putu Adhi Wijaya, Hendy Young, Andre Yulia Widyasari