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Jurnal Bios Logos
Published by Universitas Sam Ratulangi
ISSN : 20889569     EISSN : 26563282     DOI : https://doi.org/10.35799/jbl.11.2.2020.28153
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
JURNAL BIOS LOGOS is the journal published by Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The aims of the journal are to publish original research papers and article review in biology science i.e. botany, zoology, molecular biology, microbiology, ecology, diversity and conservation, taxonomy and biogeography. BIOS LOGOS is published two times per year (February and August)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 273 Documents
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Identifikasi Trichoderma sp. dari Rizosfer Tanaman Kanonang (Chordia dichotoma. G. Forst) dan Karakteristik Pemacu Pertumbuhan serta Daya Antagonismenya Terhadap Fusarium oxysporum Billy David Rawis; Dylan R. Pahlevi; Parluhutan Siahaan; Marina Singkoh; Agustina Tangapo
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.60630

Abstract

The use of biological control agents to inhibit the growth of pathogens in this case Fusarium in agricultural plants is very important in order to reduce the impact of environmental damage. Trichoderma sp. is a fungus that can be obtained from the rhizosphere of plants that can increase soil fertility and inhibit pathogenic fungi that cause damage to agricultural plants. The use of local isolates of antagonistic fungi in this case from the rhizosphere of the Kanonang plant gave results where the discovery of Trichoderma species that are able to control plant pathogens in this case Fusarium oxysporum through antagonism mechanisms such as inhibition, antibiosis and microparasitism. To find out the species of Trichoderma sp, it was continued with a molecular test using the ITS marker gene and the results of the test confirmed that the Trichoderma species in the rhizosphere of the Kanonang plant was the Trichoderma asperellum type. This type of Trichoderma also after being tested for its characteristics in stimulating growth gave positive results for both phosphate solvent and auxin producer (IAA) tests
Effectiveness of Agarwood Formation Using a Fungal Consortium on Three Stem Diameter Sizes of Aquilaria malaccensis Lamk. in Banyuwangi Apra Humaera; Sentot Adi Sasmuko; Aida Muspiah; I Made Nanda Pradita; Tri Mulyaningsih
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.61086

Abstract

Agarwood is a wood containing fragrant resin produced as a response of tree defense against disease. One of the artificial techniques that can be used for agarwood induction is inoculation, which is a technique of inserting pathogens (inoculants) into the stem such as fungi as a disease to accelerate the stimulation of agarwood formation. This study aims to analyze the size of the stem diameter against the length of infection propagation, the thickness of aromatic resin propagation, resin color and anatomical characteristics of agarwood to see the distribution of resin in the tissue, after being induced by a fungal consortium. Induction in this study was carried out on 3 sizes of tree trunk diameters (10 cm, 15 cm, and 20 cm) with 3 repetitions. Data were analyzed using analysis of variance (ANOVA) and qualitative descriptive for resin anatomy and color. The longest propagation of aromatic resin was obtained on a stem with a diameter of 15 cm. The aromatic resin that produced the darkest color was obtained from a stem with a diameter of 10 cm. The longest infection propagation was obtained on a stem with a diameter of 20 cm.The distribution of aromatic resin in the tissue accumulates in the pith rays, interxilary phloem, trachea, and tracheids.
Correlation of Body Mass Index (BMI) with Forced Vital Capacity (FVC) and Forced Expiratory Volume 1 Second (FEV1s) in Adolescents Hedison Polii; Joice. N.A Engka; Youla A. Assa
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.62054

Abstract

The Respiratory System plays an important role for humans. The main function of the respiratory system is to obtain oxygen (O2) required by the body’s cells and to expel carbon dioxide produced by the cells. Body Mass Index (BMI) influences lung volume. Forced Expiratory Volume in 1 second (FEV1) is the lung capacity that includes the volume of air that can be forcefully exhaled in one second during a pulmonary function test. FEV1 is often used as an important parameter in evaluating lung function. This study aims to investigated the relationship between Body Mass Index (BMI) and Forced Vital Capacity (FVC), as well as Forced Expiratory Volume in 1 Second (FEV1), in adolescents. This research is an analytical observational study using a cross-sectional research design. Sampling was performed using purposive sampling techniques. Data collection involved measuring body weight and height to calculate BMI and measuring Forced Vital Capacity (FVC) using a spirometer. Data analysis was conducted using normality tests and Spearman’s correlation test. The study results revealed correlation between Forced Vital Capacity (FVC) and Body Mass Index in adolescents. Other results showed that there was no statistically significant relationship between Body Mass Index and FEV1s in adolescents.
Induksi Kalus Batang Kumis Kucing (Orthosiphon stamineus Benth.) dengan Penambahan Pikloram dan BAP Zidna Sahla Mazida; Noor Aini Habibah
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.62287

Abstract

Tanaman kumis kucing (Orthosiphon stamineus) mempunyai beragam kandungan metabolit sekunder. Metabolit sekunder dapat diproduksi melalui teknik kultur kalus dengan penambahan zat pengatur tumbuh (ZPT). Tujuan dari penelitian ini adalah untuk menganalisis pengaruh kombinasi pikloram dan BAP terhadap induksi kalus pada eksplan batang tanaman kumis kucing. Rancangan penelitian yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) satu faktor yaitu berupa jenis/konsentrasi ZPT. Data kuantitatif meliputi waktu muncul kalus, persentase eksplan berkalus, dan pertumbuhan kalus (berat basah dan berat kering) dianalisis menggunakan uji Kruskall-Wallis dan dilanjut uji Dunn. Data kualitatif meliputi morfologi kalus (tekstur dan warna) dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa kombinasi pikloram dan BAP berpengaruh terhadap waktu muncul kalus, persentase berkalus, dan pertumbuhan kalus. Rerata waktu muncul kalus tercepat yaitu 6.25 HST pada 1 ppm pikloram + 0 ppm BAP. Perlakuan dengan kombinasi pikloram + 2 ppm BAP merupakan yang paling optimal dalam menginduksi kalus batang kumis kucing dengan persentase 100 %. Rerata berat basah kalus tertinggi yaitu 0.195 g (3 ppm pikloram + 2 ppm BAP), sedangkan rerata berat kering kalus tertinggi yaitu 0.051 g (3 ppm pikloram + 3 ppm BAP). Sebagian besar kalus yang dihasilkan berwarna hijau kekuningan dengan tekstur remah.
Novel Innovations in Herbal Wine and Pharmacological Potential Dwi Rahayu Pujiastuti; Mario Nikolaus Dalengkade
JURNAL BIOS LOGOS Vol. 15 No. 1 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i1.62572

Abstract

In recent years, the wine industry has undertaken various innovations to support the development of functional foods, as evidenced by numerous wine products formulated with herbal plants. These combinations have resulted in a diversity of secondary metabolites, which are of significant interest to the pharmaceutical industry. Nevertheless, further breakthroughs are needed to introduce novelty in herbal wine products. One such innovation involves the utilization of Cymbopogon citratus, Zingiber officinale, Citrus hystrix, Elettaria cardamomum, Cinnamomum verum, Syzygium aromaticum, and Caesalpinia sappan L. This study identified 85 compounds, demonstrating distinct differences in the chemical of bioactive compounds between the raw herbal plants and the fermented product. These differences are attributed to substitution reactions occurring in the carbon framework, resulting in the formation of derivative compounds in the herbal wine. Additionally, enzymatic reactions mediated by Saccharomyces cerevisiae during fermentation led to the formation of biologically important precursors, such as N-Acetylmannosamine, which plays a role in the regulation of GNE gene mutations. Therefore, the herbal wine produced in this study presents potential as a functional food with promising pharmacological applications.
Antagonist Test of Gliocladium sp. against Fusarium sp. Cause of Wilt Disease in Shallot Plants In Vitro Dea Sarayar; Stella Deiby Umboh; Parluhutan Siahaan
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.62836

Abstract

This study aims to analyze the morphological characteristics of the fungus Gliocladium sp., measure its inhibition against the growth of the fungus Fusarium sp. causing wilt disease in shallot plants (Allium ascalonicum L), and examine its antagonistic mechanism in vitro. This research method includes exploration and sampling, sterilization of tools and materials, making PDA media, isolation, purification, and identification of morphological characteristics and measurement of antagonistic fungal inhibition. The antagonist test uses the dual culture method, and data analysis with a completely randomized design (CRD) and ANOVA test followed by the Honestly Significant Difference (HSD) Test at the 5% significance level. The results showed that Gliocladium sp. was able to inhibit the growth of Fusarium sp. with a percentage inhibition of 25.63% and spore viability of 66.52%. Antagonistic mechanisms observed include competition for space and nutrients, antibiosis, lysis, and mycoparasitism.
Antagonist Activity of Gliocladium sp. Against Fusarium Wilt Fungi in Chili Plant (Capsicum frutescens L.) Cheryl Pijoh; Feky Recky Mantiri; Song Ai Nio
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.62910

Abstract

Fusarium sp. is a pathogen that causes wilt disease in chili plant (Capsicum frutescens L.), which has a significant impact on crop production. One of the environmentally friendly control strategy is the use of biological agents, such as Gliocladium sp. This study aimed to evaluate the antagonistic ability of Gliocladium sp. to inhibit the growth of Fusarium sp. in vitro. The experiment was conducted using the dual culture method on Potato Dextrose Agar (PDA) medium. Fusarium sp. isolates were obtained from the culture collection of BPPMTPH Kalasey. Observations were carried out of seven days to assess colony growth and calculate the percentage of inhibition. Gliocladium sp. demonstrated the ability to inhibit Fusarium sp. growth, with a maximum inhibition percentage of 51.52% on the seventh day. Gliocladium sp. exhibited rapid growth and dominated the medium, covering the pathogen colony entirely, although no clear inhibition zone was formed. The antagonistic mechanism is presumed to involve competition for space and nutrients, and parasitism. The mechanism of competition was demonstrated by the ability of Gliocladium sp. to grow more rapidly and dominate the PDA medium compared to Fusarium sp. in the dual culture treatment. Parasitism was identified through the coiling of Fusarium sp. hyphae by Gliocladium sp. hyphae, accompanied by morphological abnormalities observed in Fusarium sp. These findings indicate that Gliocladium sp. has potential to be developed as a biological control agent against Fusarium wilt desease in chili plants.
Uji Aktivitas Hepatoprotektor pada Tikus Model Kanker Payudara yang Diinduksi Benzo(A)Piren Setelah Pemberian Ekstrak Metanol Daun Biwa (Eriobotrya japonica Lindl) Fitri Elizabrth Hasibuan; Widya Syahfitri; Salomo Hutahaean; Syafruddin Ilyas; Febry Rahmadani Hasibuan
JURNAL BIOS LOGOS Vol. 15 No. 1 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i1.63461

Abstract

This study aimed to evaluate the hepatoprotective activity of methanolic extract of loquat (Eriobotrya japonica Lindl) leaves in breast cancer model rats induced by benzo(α)pyrene. This study used a completely randomized design with five groups: Group I as the negative control, Group II as the positive control (administered BaP 50mg/KgBW), and three treatment groups receiving methanolic extract of loquat leaves at doses of 200 mg/kg BW, 300 mg/kg BW, and 400 mg/kg BW for 30 days. Blood serum samples from the breast cancer model rats were analyzed to determine SGPT and SGOT levels using the IFCC method. Data were statistically analyzed using Analysis of Variance (ANOVA) followed by Tukey’s HSD post-hoc test. The results showed no significant differences between the treatment and control groups for SGOT (F(4,20) = 0.7123, P = 0.593) and SGPT (F(4,20) = 0.4460, P = 0.774) levels. Based on these findings, it can be concluded that administration of methanolic extract of loquat leaves did not cause liver function impairment in the breast cancer model rats. These results support the potential of loquat leaf extract as a hepatoprotective agent, particularly in maintaining stable SGPT and SGOT levels in breast cancer conditions.
Anatomical Study of Pigmented Rice Leaves (Oryza sativa L.) Resistant to Blast (Pyricularia grisea Sacc.) Disease Pingkan Krista Mantiri; Fione Yukita Yalindua
JURNAL BIOS LOGOS Vol. 15 No. 1 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i1.63500

Abstract

Pigmented Rice (Oryza sativa L.) is one of Indonesia’s local crops with potential to be developed as a source of healthy food. A major constraint in pigmented rice cultivation is the emergence of the fungus Pyricularia grisea (Sacc.), which causes blast disease. This pathogen attacks the leaves, leading to growth damage and even plant death. All plants inherently possess defense mechanisms against external threats, whether biotic (insects, fungi, bacteria, viruses) or abiotic (environmental stress). These mechanisms involve anatomical response. Previous research identified a pigmented rice variety, Cempo Ireng, that exhibits resistance to blast disease caused by P. grisea. Pigmented rice is also known to contain high levels of phenolic compounds and flavonoids, which contribute to plant defense and coloration. This study aims to determine the total phenolic and flavonoid content in the leaves of blast-resistant and susceptible pigmented rice varieties infected by P. grisea (Sacc.). Two pigmented rice varieties were tested: Cempo Ireng (resistant) and Indmira Hitam (moderately resistant). Control Varieties: Asahan (blast-resistant) and Kencana Bali (susceptible). Based on the results of this study, Cempo Ireng and Asahan showed strong resistance to blast disease, Indmira Hitam exhibited moderate resistance and Kencana Bali was highly susceptible to P. grisea infection. Anatomical observations of leaves using leaf surface section showed differences in structural modification of epidermal thickness, mesophyll thickness and the diameter of vascular bundle, where Cempo Ireng varieties were able to form good epidermal thickness as the initial barrier of defense. The anatomical observations of leaves using SEM showed anatomical differences in trichome density in Cempo Ireng and Asahan leaves which had the highest density compared to other varieties in response to the attack of fungal pathogens.
Isolation, Screening, and Optimization of Amylase-Producing Endophytic Bacteria from Rhizophora apiculata Agustina Monalisa Tangapo; Susan Marlein Mambu; Pience Veralyn Maabuat; Dwina Kristianti; Williando Ering; Lalu Wahyudi
JURNAL BIOS LOGOS Vol. 15 No. 1 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i1.63548

Abstract

High amylase enzyme production requires optimal supporting factors for enzyme production. The purpose of this study was to determine the optimum conditions for endophytic bacterial fermentation, including substrate concentration, pH, and fermentation temperature, for the production of amylase from endophytic bacteria of mangrove plants Rhizophora apiculata. Analysis of amylase enzyme activity was carried out using the 3,5-dinitrosalicylic acid (DNS) assay. The results obtained from this research were from a total of 18 endophytic bacteria R. apiculata that were successfully isolated, 6 bacterial isolates were obtained which showed the ability to produce extracellular amylase. The potential of bacteria for amylase production grown on 0.5% starch media was determined based on the ratio between the diameter of the halo zone and the diameter of the bacterial colony. Three isolates were selected, EB.5, EB.10, and EB.12 for further optimization of the fermentation conditions for amylase production. Based on the results obtained, it was shown that the fermentation conditions for amylase enzyme from the three isolates of endophytic bacteria were optimum in media with a concentration of 1.5% starch, in media with a pH of 6, and optimum in the temperature range of 30oC.

Page 26 of 28 | Total Record : 273

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