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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 15 Documents
 1   2 
Search results for , issue "Vol 9 No 2 (2024): November" : 15 Documents clear
Mangifera spp. in the Ethnoclimatology Study of Tatangar Banjar Tradition in Kalimantan Hidayati, Eisya Hanina; Yuniati, Ratna; Lestari, Retno; Jumari, Jumari; Walujo, Eko Baroto
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30048

Abstract

The biodiversity of Mangifera spp. not only supports the local economy and preserve food security but also safeguards cultural heritage through their role in ethnoclimatology studies within the Tatangar tradition of the Banjar community in Kalimantan. The Banjar community demonstrates a unique connection with nature by utilizing specific Mangifera species as crucial indicators of seasonal transitions, deeply ingrained in their cultural identity. The aim of this study is to reveal the prevalence of various Mangifera species, including endangered ones, in diverse landscapes, promoting a sustainable agroforestry system and conservation efforts. Qualitative and quantitative research methods were employed to explore and analyze Tatangar knowledge within the Banjar community in Mandiangin Barat Village, particularly its connections to climate and weather. The Banjar community further traditionally classifies Mangifera species, or known as “asam-asaman”, based on morphological traits, aiding in their interpretation of Tatangar signs. These signs, rooted in the flowering time of Mangifera trees, serve as essential climate indicators, guiding agricultural practices and influencing decisions on planting and harvesting. This local knowledge enhances agricultural resilience, enriches their cultural heritage, and underscores the intrinsic relationship between culture, nature, and sustainability in Kalimantan, making it a valuable subject for ethnoclimatological exploration
Effectiveness of Fruit Peels Eco Enzyme in Reducing Total Chromium from Yogyakarta Tanning Industry Waste Putri, Caecilia Radella Rivika; Jati, Alphonsus Wibowo Nugroho; Yulianti, Lorensia Indah Murwani
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.31077

Abstract

. Leather tanning industry is essential in Indonesia's economic sector, specifically in the export of hides and leather goods. The high production of goods is often accompanied by a large amount of waste disposal, containing elevated levels of chromium which has a negative effect on the environment. To address this problem, eco enzyme containing high carboxyl groups is expected to be an effective solution. Therefore, this study aimed to determine the functional groups in fruit peels eco enzyme and its effectiveness in reducing total chromium content in leather tanning waste. The experimental process included the preparation of eco enzyme formulations, testing functional groups with FTIR, and determining total chromium content using AAS. Furthermore, the characteristics of waste were determined, including such as BOD, pH, temperature, ammonia, TDS, and TSS, followed by incubation of eco enzyme in waste, as well as data analysis with ANOVA and Duncan test. The results showed that eco enzyme formulations contained hydroxyl (O-H) and carboxyl (-COOH) functional groups, carbon-carbon single bonds (C-C), carbon-oxygen single bonds (C-O), carbon-carbon double bonds (C=C), and carbon-hydrogen bonds (C-H). All formulations effectively reduced chromium levels in leather tanning waste to value lower than quality standards. Based on the results, the best formulation was found to be eco enzyme A, which comprised one type of fruit, namely mango peels.
Phytochemical Constituent, Antibacterial and Antioxidant Leaves of Dracaena trifasciata (Prain) Mabb. Dewatisari, Whika Febria; To'bungan, Nelsiani
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33528

Abstract

Sansevieria, commonly known as Dracaena trifasciata. Apart from serving as an ornamental and air-purifying plant, it also exhibits medicinal properties, making it a potential candidate for the development of novel natural drugs. The demand for natural antibacterial agents has significantly increased due to the rising incidence of resistance among pathogenic bacteria, such as Staphylococcus aureus. This research conducted to determine the antibacterial and antioxidant activities of D. trifasciata leaf extracts. The D. trifasciata leaf material was sequentially extracted using sonication, beginning with chloroform and then followed by ethanol. The Kirby-Bauer method was employed to conduct the antibacterial test, wherein the inhibition zones generated by the chloroform and ethanolic extracts were measured. The test bacteria used in this study was Staphylococcus aureus. The assessment of the antioxidant activity was conducted using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Phytochemical compound identification in the extracts was conducted using GC-MS analysis. The ethanolic extract showed a higher yield percentage compared to the chloroform extract. Both extracts exhibited moderate antibacterial activity. The chloroform extract exhibited an inhibition zone of 73.33 mm2, while the ethanolic extract showed an inhibition zone of 110 mm2. The chloroform extract had a minimum inhibitory concentration (MIC) of 500 mg/mL and a minimum bactericidal concentration (MBC) of 1000 mg/mL. The ethanolic extract had an MIC of 31.25 mg/mL and an MBC of 62.50 mg/mL. In terms of antioxidant activity, the chloroform extract had an IC50 of 370.8±0.07 µg/mL, while the ethanolic extract had an IC50 of 647.4±0.12 µg/mL. GC-MS analysis revealed 47 compounds in the chloroform extract and 49 compounds in the ethanolic extract. Based on the results of yield, antibacterial and compound identification, the ethanolic extract of D. trifasciata leaves exhibited greater potential compared to the chloroform extract. Its antibacterial activity showed more promise than its antioxidant activity
Effects of Wedelia Ethanol Extract on Sperm Quality and Blood Cholesterol Levels in Obese Rats Iffatuzzahra', Yumna Nabilah; Wardani, Diah Kartika; Hidayat, Andika Gundawa; Makhabati, Ulinuha Farah; Setiawan, Haris
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.37673

Abstract

Obesity is a complex, multifactorial condition contributing to male infertility by increasing oxidative stress, which negatively affects reproductive hormones, sperm quality, and blood cholesterol levels. The Wedelia plant (Sphagneticola trilobata (L.) Pruski) is a natural herbaceous plant containing flavonoids and tannins, known for enhancing sperm quality and inhibiting cholesterol synthesis in the blood. This study aims to determine the potential of Wedelia in improving sperm quality and regulating blood cholesterol levels. The study used 24 male Wistar rats divided into four groups, consisting of C (no HFF, distilled water only), NC (HFF, distilled water), T1 (HFF and 100 mg/Kg BW Wedelia extract), and T2 (HFF and 200 mg/Kg BW Wedelia extract). HFF was administered from weeks 1 to 3, followed with extract treatments from weeks 3 to 5. On day 36, the rats were euthanized for blood collection using hematocrit into EDTA tubes. The rats were dissected for the epididymis, which was then incised in a 10% PBS to release sperm. Sperm motility and count were observed on glass slides, while sperm viability and morphology were analyzed with hematoxylin-eosin using an Optilab microscope (400x magnification). The observation parameters included sperm morphology, count, motility, viability, total cholesterol, LDL, and HDL levels. Data were analyzed using ANOVA with DMRT post hoc testing (p ≤ 0.05). The results showed that T2 exhibited an increase in sperm count, motility, viability, and morphology in HFF-induced obese rats. T1 and T2 also showed enhanced HDL cholesterol and reduced total and LDL cholesterol levels. In conclucions, a Wedelia leaf extract dose of 200 mg/kg BW optimally improves sperm quality and reduces total and LDL cholesterol levels.
Investigating Species Composition and Abundance of Marine Microalgae from Temajo Island Waters, West Kalimantan, Indonesia Safitri, Ikha; Kushadiwiojayanto, Arie Antasari; Sofiana, Mega Sari Juane; Nguyen, Duc-Hung; Adjout, Rebiha
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.38028

Abstract

Temajo Island is renowned for its marine tourism activities and is home to a range of organisms, including algae. Microalgae serve as indicator species, offering insights into the aquatic environment through their species composition and abundance. This study aims to provide preliminary data on the abundance and some ecological indices (diversity, evenness, and dominance) of microalgae on Temajo Island, which will be the basis for assessing water conditions and supporting conservation efforts and sustainable management of natural resources. The research was carried out at four stations with different characteristics. At each station, surface water samples were collected using a plankton net. The microalgae in these samples were subsequently examined under a microscope, and their quantities were measured. The microalgae abundance ranged from 2.84 to 7,697.14 ind/L, with an average of 193.34 ind/L. Chaetoceros was the most abundant genus, followed by Rhizosolenia, Guinardia, Thalassiosira, Pseudo-nitzschia, and Bacteriastrum. The diversity index (H’) ranged from 2.67 to 3.08, indicating moderate to high species richness, while the evenness index (E) ranged from 0.64 to 0.74, reflecting a high level of uniformity among microalgae populations. The dominance index (C) varied between 0.11 and 0.20, suggesting low dominance by any single genus. These indices collectively indicate a balanced and diverse microalgae community, underscoring the ecological health and stability of the Temajo Island waters. Preliminary information about the species composition and abundance of marine microalgae in this study provides valuable insights into the dynamics of microalgae populations and their ecological implications in the marine ecosystems of Indonesia.
Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli Inggrid, Annabelle Marie; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30436

Abstract

Sapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 ± 0.70 mg QE/g, 1.76 ± 0.16 g GAE/g, and 1.37 ± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli.
Characterization of Orexin Gene in Tilapia (Oreochromis niloticus):Regulator Feeding Appetite, and Correlation with Reproductive Factors Simanjuntak, Ricky Febrinaldy; Barlian, Anggraini; Wibowo, Indra; ., Rukisah
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.32063

Abstract

The mechanism of energy allocation related to increased appetite and feed intake in fish involves several neuropeptides encoded by specific genes. Orexin (OX) is a peptide secreted in the pars tuberalis of the hypophysis that acts as a stimulator in appetite increase (orexigenic factor). However, the expression of different orexigenic factors varies among vertebrate species, reflecting their unique types and lineages. Therefore, this study aimed to identify genes encoding appetite in Tilapia. RNA isolation, complementary Deoxyribose Nucleic Acid (cDNA) cloning, and DNA amplification were performed from brain samples of gonadally mature Tilapia. The PCR products were subsequently sent to Macrogen.Inc for sequencing. The amplification results of Orexin with β-Actin (positive control) observed using agarose gel electrophoresis showed that the size of the nucleotide base product of each gene was 196 bp and 197 bp. Confirmation of sequencing results carried out using the Basic Local Alignment Search Tool (BLAST) method - National Center for Biotechnology Information (NCBI) for Orexin and β-Actin were 97% and 100%. Based on these results, it can be concluded that each target gene isolated from the tilapia brain showed homology/similarity with the sequence available in the NCBI database
The Growth of Oyster Mushroom on Ramie Chip Waste-Derived Media was Accelerated by Rice-washed Water Budiono, Ruly; Washfanisa, Handina Alya; Mutaqin, Asep Zainal; Kusmoro, Joko; Nurzaman, Mohamad; Setiawati, Tia; Hasan, Rusdi
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33433

Abstract

Rice-washed water is wastewater resulted from the rice cleaning process that is rich in nutrients. The use of ramie chip waste can be considered a potential substrate due to its lignocellulosic nature, similar to other wastes studied. This study aimed to examine the effect of rice-washed water on the mycelium and pinhead growth of white oyster mushrooms grown in the ramie chip waste as the growing medium. The experiment was conducted for three months using a completely randomized design with four rice-washed water treatments and six replications. The rice-washed water concentration treatments were 0% (K0), 25% (K1), 50% (K2), and 100% (K3), which were added to the ramie chip waste as the growing medium for white oyster mushroom. The time of mycelium propagation velocity, the time the first pinhead appearance, the number of mushroom bodies, and the largest cap diameter were observed. The results showed that ramie chip waste-derived media was suitable to cultivate white oyster mushrooms. Adding 100% rice-washed water to the media significantly affected the speed of mycelium propagation (16.51 days) compared to control (30 days) and the appearance of the first pinhead (31.5 days) compared to control (38.5 days) as well as the number of fruiting bodies of mushroom (8.8) compared to control (6.2). However, the rice-washed water application did not significantly affect the diameter of the largest mushroom cap.
Analysis of Bacterial Diversity In Temple Bricks Using Phenetic Numeric Taxonomy Method Alifia, Luluk; Zulaika, Enny; Soeprijanto, Soeprijanto
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33476

Abstract

Temple bricks-based constructions are known to often face various challenges, including physical, chemical, and biological weathering. Previous studies identified various biological factors contributing to brick weathering including exudate produced by microorganisms, such as bacteria. In addition, bacteria often live synergistically and antagonistically with other species, exhibiting diverse morphological and physiological traits (bacterial diversity). Various methods have been developed to explore bacterial diversity, with phenetic numerical taxonomy being the most popular. Therefore, this study aims to determine bacterial diversity on the surfaces of temple bricks using phenetic numerical taxonomy method. Bacterial isolation was carried out aseptically, followed by labeling and transferring the isolate to the laboratory for further tests. The tests carried out included morphological characterization, biochemical assays, physiological reactions, and potential enzymatic activities. Subsequently, dendrogram was constructed using MVSP (Multi-Variate Statistical Package) software with isolated grouping based on the Unweighted Pair Group Method Averages (UPGMA) algorithm. The similarity between isolates was analyzed using the Simple Matching Coefficient (SSM) similarity value. The dendrogram analysis revealed the presence of 3 clusters namely A (4 isolates), B (1 isolate), and C (2 isolates), with a similarity index of 0,543 to 0,857. Clusters A, B, and C had a similarity index of ≤0.700, indicating the occurrence of distinct species in each cluster. Based on the profile-matching results of key characters, the 7 bacterial isolates were identified as belonging to the genera Bacillus, Corynebacterium, and Mycobacterium.
Analysis of the Molecular Structure of Lipase-Dependent Chaperone from Ralstonia pickettii Strain BK6 Azwar Syah, Muhamad; Ambardini, Sri; Jamili, Jamili; Muzuni, Muzuni; Trisandy, Darul
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33770

Abstract

Several biotechnology industries are exploring the characteristics of lipase-dependent chaperones due to their distinctive biochemical traits. This study aimed to employ bioinformatics to analyze the molecular structure of Ralstonia picketii BK6's lipase-dependent chaperon (LipRM). The sequence mapping and amino acid distribution were examined using BioEdit (version 7.0.9.1). SignalP 5.0 and Interpro are employed for signal peptide detection, whereas Swiss-Model and VMD 1.9.2 are used for molecular dynamics modelling. The results showed that the Shine-Dalgarno sequence was discovered in the LipRM promoter, seven nucleotides upstream of the initiation codon (AUG) with the 5'-AGGAGA-3', and has a terminator region that facilitates the formation of a secondary structure. The protein's 3D structure prediction results indicate differences in the alpha helix chains (residues 166-174 and 254-271) between LipRM and the reference lipase. LipRM's molecular structure comprises a detachable signal peptide, and with variations in helix alpha chain conformation and ligand geometry.

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