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INDONESIA
BIOEDUSCIENCE
Published by Universitas Muhammadiyah Prof. Dr. Hamka
ISSN : 2614154X     EISSN : 26141558     DOI : -
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education
BIOEDUSCIENCE is an open access journal that publishes research in the field of Biology and Biosain Education such as: Applied and implemented in education and learning, Botany, Zoology, Microbiology, Ecology, Biotechnology, Molecular Biology, Genetics, Bioinformatics, Cell and Developmental Biology, Biodiversity and Bioconservation. BIOEDUSCIENCE is published by Biology Education Study Program, Universitas Muhammadiyah Prof. Dr. Hamka, Indonesia regularly in June and December. ISSN : 2614-1558; P-ISSN : 2614-154X.
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Articles 544 Documents
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The Emergence and Impact of Artificial Intelligence in Biotechnology Ali, Jahanzaib
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12578

Abstract

Introduction: Artificial intelligence (AI) has transformed many fields, including biotechnology. In recent years, artificial intelligence (AI) has played a critical role in altering research, drug development, genetic analysis, customized treatment, and other fields. The purpose of this review article is to investigate the numerous uses of AI in biotechnology, focusing on its impact on accelerating scientific advancement, improving data analysis, and driving innovation in the field. Method: Various AI approaches and methodologies used in biotechnology will be explored, including machine learning, deep learning, natural language processing, and picture identification. Results: This essay will also address important problems, future potential and ethical concerns regarding the use of AI in biotechnology. Conclusion: The integration of AI in biotechnology has redefined research paradigms, data analysis, and decision-making processes.
Metabolit Sekunder Bacillus sp. sebagai Antijamur Jamur Patogen Tular Benih pada Benih Jagung dengan Metode Uji Blotter Setyowati, Laras; Purnawati, Arika; Mujoko , Tri; Mukaromah, Fathul
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12588

Abstract

Background: Seed is a planting material that must have high viability and quality, including being free from pathogens. One of the obstacles in the supply of quality maize seeds is seed-borne fungal pathogen which can affect the quality of maize seeds and cause plant disease. Endophytic bacteria Bacillus sp. has been widely studied to be able to produce secondary metabolites as antifungals. This study aimed to determine the exact concentration of secondary metabolites of endophytic bacteria Bacillus sp. to decrease the infections of seed-borne pathogens fungal on maize seeds using the blotter test method. Methods: This study used a completely randomized design with four concentration levels (10%, 15%, 20%, 25%) and two strain codes of endophytic bacteria Bacillus sp. (Bth-31a and Bth-22). Results: The result of identifying seed-borne fungal pathogen on maize seed included Fusarium sp., Aspergillus flavus, Aspergillus niger, and Rhizopus sp. The Bth-22 treatment with a concentration of 25% could decrease the infection of seed-borne fungal pathogen and had the highest percentage until 50% compared to the control. While the lowest percentage to decrease the infections of seed-borne fungal pathogen occurred in the Bth-31a treatment with a concentration of 10%, which was 32.1% compared to the control. Conclusions: All seed treatments that used secondary metabolites of Bacillus sp. able to decrease the infections of seed-borne fungal pathogen on maize seed compared to the control. In addition, the treatment of secondary metabolites Bacillus sp. is able to increase the germination of maize seeds.
The Characteristics of Solid Soap with Varied Additions of Kefir Cheese Whey Solikah, Solikah; Kurniawan, Kurniawan; Husna, Ashiqotul; Nuha Pitaloka, Isyana
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12681

Abstract

Background: Kefir cheese whey is a product of fermented cheese whey wastewaste. Kefir cheese whey has been known to contain Lacofferin. Lactoferrin functions as an antimicrobial. Methods: This study focuses on producing solid soap from kefir cheese whey. The saponification reaction of saponification reaction of coconut and palm oil, with Natrium Hidroxide with kefir cheese whey, initiated the solid soap production. Results: show that all formulae of solid soap from kefir cheese whey showed good physicochemical characteristics, pH 10.09–10.98 (ASTM requirement 9-11), foam content 10.98-12.89. Soap Kefir cheese whey can be concluded that all formulas meet the requirements that all formulas meet the requirements established by SNI 3532:2016 and ASTM D 2022. Conclusions: The Best formula for solid soap variant kefir is 100 % whey. Thus, the giving of kefir whey is recommended for manufacturing kefir soap. It suggests that soap kefir cheese whey is recommended for use as an antibacterial agent in the manufacture of solid soap.
Skrining Bakteri Lipolitik dari Tanah Pemakaman Mandiri, Listy Hasti; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12686

Abstract

Background: The cemetery is an area for the burial of corpses so that active decomposition of bodies occurs. One of the decomposition bacteria that might be found is lipolytic bacteria because the human body comprises 21,33-32,51 % lipids. Explorations of lipolytic bacteria from cemeteries in Indonesia have not been found, so this research needs to be done. This non-experimental study aimed to select and identify lipolytic bacterial isolates from the Pracimaloyo cemetery, Surakarta. Method: A total of 36 bacterial isolates were selected for their lipolytic activity using tributyrin media. The lipolytic index determines lipolytic activity. Identification of lipolytic bacteria based on colonial morphology and Gram staining. Result: The study showed that 22.2% (8 isolates) showed a lipolytic positive, with the largest lipolytic index (LI) value of 2.5 (isolate P36). Bacterial colonies are circular-shaped, have entire edges, flat elevation, and are yellow or white. Gram staining results showed that the isolated is a group of Gram-negative bacteria in the form of coccus. Conclusion: The conclusion of this study suggests that bacterial isolates from Pracimaloyo cemetery that have potential lipolytic activity, which is likely to be from the genera Pseudomonas and Klebsiella, were obtained.
CRISPR-Cas9 System In Vivo Delivery to Combat HBV Ali, Jahanzaib; Aslam, Husnain; Yousuf , Abida
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12693

Abstract

Background: Hepatitis B Virus (HBV) infection remains a major global health issue despite the availability of HBV vaccine. The novel CRISPR-Cas9 gene editing technology efficiently helps to cure HBV by disruption or cleavage of HBV DNA. Aims: Several in vitro and in vivo studies have demonstrated the effectiveness of HBV-specific clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (CRISPR/Cas9) systems in cleaving HBV DNA. Methods: In vivo, delivery of the CRISPR/Cas9 system at target sites remains a major challenge that needs to be resolved before its clinical application in gene therapy for HBV. Results: In this review article, we comprehensively evaluate the progress, challenges, and therapeutic potential of CRISPR-Cas9 gene therapy for HBV using adeno-associated virus (AAV) vectors as delivery vehicles. Conclusion: The CRISPR-CAS9, HBV, AAV, delivery methods of CRISPR-CAS9 component in vivo, challenges, and future perspectives in harnessing this innovative technology to combat HBV infection.
Eksplorasi dan Implementasi Mikroba Indigenous Asal Limbah Batubara Sebagai agen Bioakumulator Terhadap Logam Berat Cd dan Pb Elawati, Nunung Eni; Lestari, Catur Retno; Resputri, Fibra
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12709

Abstract

Background: Heavy metals in the environment are a very important environmental pollution problem. Degrading and removing heavy metals does not degrade organic waste because heavy metals are non-biodegradable. Bioremediation is one technique that can be chosen to degrade metal waste. The purpose of this study was to obtain and identify native bacteria from coal waste that have the potential to reduce heavy metals or bioaccumulation. Method: samples were taken from coal waste, then isolated using the dilution method and incubated at 37˚C for 24 hours. Testing for heavy metal content by applying a microbial consortium suspension on coal waste with a mixture of planting media, then analyzed with an atomic absorption spectrophotometer. Results: 10 isolates with different color characteristics were obtained from microbial isolation. The analysis of heavy metal content showed a decrease in the heavy metal Pb in the L1 and L2 treatments but did not affect the heavy metal Cd. Conclusion: The microbial consortium isolated from PLTU coal waste shows a decrease in the heavy metal Pb in treatments L1 and L2.
Aktivitas Keluar Sarang Heterotrigona itama Cockerell. (Hymenoptera: Apidae: Meliponinae) di Taman Hutan Raya Pocut Merah Intan, Kabupaten Aceh Besar, Aceh, Indonesia Suwarno, Suwarno; Akbar, Novrizal; Rizki, Alia; Siregar, Zuriana
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12739

Abstract

Background: The study conducted in the Pocut Meurah Intan Forest Park (TAHURA PMI) area aimed to analyze the outgoing activity of Heterotrigona itama worker beehives and their relationship with environmental and physical factors such as temperature, humidity, and light intensity. Methods: H. itama activity is observed from 06.00 to 18.00 WIB. Bees exiting the hive are counted using manual counting to determine the number of individuals that exit the hive for 10 minutes every hour. Observation of activities outside the nest consists of foraging, throwing garbage, and drones guarding the nest. Results showed that the peak of activity out of H. itama nests occurred at 09.00 - 10.00 WIB and 13.30 - 14.30 WIB. Results: Foraging activity was the highest nest exit activity. The relationship between light intensity factor and activity outside the foraging nest is moderate (R2 = 0.651), while temperature and humidity are weak (R2 = 0.052 and 0.091). The activity of exiting the nest to throw garbage has a moderate relationship with light intensity (R2 = 0.439) compared to temperature (R2 = 0.253) and humidity (R2 = 0.296). Furthermore, physical factors of light intensity (R2 = 0.6217) had a greater relationship with drone activity out of the nest than factors of temperature (R2 = 0.4385) and humidity (R2 = 0.4063). Activity outside the nest is positively correlated with temperature and light intensity and negatively correlated with humidity. Conclusions: Thus, it can be concluded that light intensity has the greatest influence on the activity out of the nest.
Isolasi Dan Uji Aktivitas Bakteri Proteolitik Yang Berasosiasi Dengan Spons Stylotella Sp. Dan Potensinya Sebagai Antibakteri Seuk, Mariana Septiani; Pardosi, Lukas; Fallo, Gergonius; Manalu, Adelya Irawan
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12980

Abstract

Background: The province of East Nusa Tenggara (NTT), Indonesia, boasts a significant expanse of marine territory, making its waters a potential treasure trove of aquatic resources. One such marine resource is the Stylotella SP sponge, which is found in this region. Sponges like Stylotella SP often have intricate relationships with microorganisms, including bacteria. Method: This research was to characterize and identify proteolytic bacteria and test bioactive compounds from isolates of proteolytic bacteria from the sponge Stylotella sp. Proteolytic bacterial strains were rejuvenated first in 2% TSA + Nacl medium and then characterized microscopically and biochemically using catalase and oxidase tests. The diffusion method used the antibacterial activity test against E.coli and S. aureus. Result: The results showed that proteolytic bacterial isolates had irregular and rounded colony shapes, wavy and intact colony edges, hilly and convex colony elevation, and white color, while biochemically, it was declared positive for the catalase and oxidase tests. Antibacterial activity tests on proteolytic bacterial strains showed the ability to inhibit the growth of Staphylococcus aureus with an inhibitory capacity of 9.5 mm and Escherichia coli with an inhibitory capacity of 7 mm.
Process Oriented Guided Inquiry Learning (POGIL): Its Effectiveness in Increasing Students' Scientific Literacy Skills in Plant Physiology Course Yennita; Astriawati, Fitri; Jumiarni , Dewi
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/13008

Abstract

Background: Today, the main goal of science learning is to create a scientifically literate society by understanding mathematics, physics, chemistry, biology, and technology. Therefore, this research aims to determine the effectiveness of the process-oriented guided inquiry learning (POGIL) model in improving students' scientific literacy skills in a Plant Physiology course. Methods: This study was a quasi-experiment using a control group pre-test and post-test design with 32 students as research subjects. Data collection techniques used tests and research data were analyzed using a one-way ANCOVA statistical test. Results: The results showed a significant difference in scientific literacy skills between the control and experimental classes by controlling for students' initial scientific literacy [F(1,61) = 1977.228, p < 0.001], effect size np2 = 0.97. Conclusions: Therefore, the POGIL model is highly effective in increasing students' scientific literacy skills in the Plant Physiology Course.
Probiotic Contents and Antioxidant Activity in Probiotic Drinks Patikala Fruit Juice (Etlingera Elatior (Jack) R.M.Smith) from Kolaka Azizah, Nur; Muktafia, Muktafia; Anjarsari, Lisa; Putri, Sartika Gunawan
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/13009

Abstract

Background: Patikala fruit (Etlingera elatior) can be used as food, medicine, and to boost the immune system. Patikala is a fruit with a high nutritional value that can be used to make a probiotic drink. The aims of this study were to see if patikala fruit juice could be used as a probiotic and antioxidant drink as an immune booster after the COVID-19 pandemic.. Methods: The patikala fruit used is typical of the Kolaka area. This research used a randomized block design using different fermentation time factors. Each treatment was carried out 3 times repetitions. Making a probiotic drink from patikala (Etlingera elatior) is by taking patikala fruit juice with a mixture of powdered skim milk and Lactobacillus casei bacteria then carrying out fermentation treatment for 20 hours, 24 hours and 28 hours. And a dilution process is carried out to test probiotic levels, and antioxidant activity tests are carried out. Results: The results obtained from the analysis of probiotic contents and antioxidant activity show that the potential of patikala fruit juice to be the best probiotic and antioxidant drink is at a time variation of 28 hours. Because during this time variation, the probiotic contents had reached a maximum total of 9.6 x 1014 cfu/ml with the best ability to ward off free radicals, 9.900% of inhibition. Conclusions: Patikala fruit juice drink (Etlingera elatior) which contains probiotics and potential antioxidant activity as a drink to improve the immune system after the COVID-19 pandemic.

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