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INDONESIA
BIOEDUSCIENCE
Published by Universitas Muhammadiyah Prof. Dr. Hamka
ISSN : 2614154X     EISSN : 26141558     DOI : -
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education
BIOEDUSCIENCE is an open access journal that publishes research in the field of Biology and Biosain Education such as: Applied and implemented in education and learning, Botany, Zoology, Microbiology, Ecology, Biotechnology, Molecular Biology, Genetics, Bioinformatics, Cell and Developmental Biology, Biodiversity and Bioconservation. BIOEDUSCIENCE is published by Biology Education Study Program, Universitas Muhammadiyah Prof. Dr. Hamka, Indonesia regularly in June and December. ISSN : 2614-1558; P-ISSN : 2614-154X.
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Articles 544 Documents
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Modeling The Habitat Suitability Of Javan Banteng (Bos javanicus javanicus) Using Geographic Information System In Ujung Kulon National Park Handayani , Handayani; Solihin, Dedy Duryadi; Alikodra, Hadi Sukadi; Jakaria, Jakaria; Rianti, Puji; Mahmud, Rois
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11547

Abstract

Background: The Banteng population in Ujung Kulon National Park (TNUK) is less than 500 individuals. The habitat of Java Banteng in conservation areas has largely decreased. One approach to assessing the current status of biodiversity at all levels, especially in endangered species, is to use geospatial technology such as remote sensing and geographic information systems combined with spatial data science. This study aims to create a spatial model of the suitability of the Javan Banteng habitat in the TNUK area and identify the use of the Java Banteng habitat and environmental variables that affect the presence of Javan Banteng. Methods: This research data was collected through coordinate data for stool sampling and data from BTNUK using a method called maximum entropy (maximum). The analysis used the Relative Use Index, Maximum Entropy modeling, and Relative Abundance Index. Results: Based on research, the use of habitat by Java Banteng with the value of making a spatial distribution model can be analyzed by analyzing the contribution of environmental variables based on the level of contribution in percent and the results of the jackknife test, namely the percentage of contribution of environmental variables in this study showed that environmental parameters, slope (37.6%) were the highest parameters, followed elevation (25.8%), land cover (25.3%), and NDVI (6%),  rivers (5.3%). The analysis of five environmental variables used in making the Javan Banteng distribution model showed that at an altitude of 45 meters above sea level, Java Banteng preferred to show 95%. The graph decreased at an altitude above 45 mdpl, and Java Banteng at 200 meters above sea level looked at 0%. Conclusions: Javan Banteng do not like or do not choose places with altitudes ranging from 200 - 625 meters above sea level.
Growth of Red Spinach (Amaranthus tricolor L.) Hydroponically Using POC Tomatoes and Moringa Leaves Pranata, Sepbrie Mulia Bingah; Suparti, Suparti
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11688

Abstract

Background: Red spinach (Amaranthus tricolor L), one of the plants contains several important elements with high nutritional value, is liked by the community, easy to cultivate with hydroponics. Efforts to determine the growth of red spinach by administering POC as a nutrient needed by plants. The purpose of this study was to find out how red spinach plants grow in POC of a mixture of tomato extract and moringa leaf extract with the addition of coconut water hydroponically and at what concentration the most effective POC. This research was conducted at the Biology Green House FKIP UMS. Methods: this study used an experimental method with a completely randomized design (CRD) with 2 factors, namely factor 1 type of POC extract mixture and factor 2 the difference in the volume of POC so that from both factors 6 treatments were obtained with three replications for each treatment. Results: Data analysis using Two Way Anova showed that the results of the C2V2 treatment had a plant height of 2.13 cm, root length of 1.96 cm, and the highest number of leaves with 4 strands. The C1V3 treatment produced the lowest plant height, namely 1.07 cm and the C2V3 treatment produced the lowest root length, namely 0.73 cm and the C1V1, C1V3, C2V1, C2V3 treatments showed the lowest number of leaves, only 2 leaves. Conclusion: The POC of tomatoes and Moringa leaves which were given the same treatment showed that the POC of Moringa leaves were effectively used as red spinach growth nutrients.
The Effect of Banana Stem Powder on the Productivity of the Brown Oyster Mushroom (Pleurotus cystidiosus) Nurhayati, Rizky; Suparti, Suparti
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11699

Abstract

Background: Brown Oyster Mushroom (Pleurotus cystidiosus) is a type of mushroom that has different advantages from other oyster mushrooms. Generally, farmers make planting media for brown oyster mushrooms made from sawdust of sengon wood. The availability of raw materials for sawdust of sengon wood is increasingly difficult to obtain. So we need other alternative materials as a source of nutrition, one of which is banana stem waste. This study aims to determine the effect of banana stem flour on the diameter of the cap, the number of fruiting bodies, and the wet weight of the fungus. Methods: This study used a completely randomized design (CRD) which consisted of one factor. This study used 4 treatments and 3 replications. Data was tested by quantitative descriptive analysis using the Analysis of Variance (ANOVA) test. Results: Banana stem flour has a significant effect on all parameters. The JM2 treatment resulted in a cap diameter of 10.3 cm, mushroom fruiting bodies of 10.6 and wet mushroom weight of 103.8 g. Conclusion: The addition of banana stem powder can increase productivity in the parameters of mushroom cap diameter, number of mushroom fruiting bodies, and wet weight of mushrooms, which can be used by readers or researchers. Utilization of waste such as sawdust of sengon wood and waste of banana fronds for the cultivation of brown oyster mushrooms is one way of handling plantation waste.
Bacteria Isolation from Public Cemeteries Soil and Test for Resistance to Antibiotics Laspartriana, Anggita Juniar; Rahayu, Triastuti; Tyastuti , Erma Musbita; Sidiq , Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11740

Abstract

Background: A public burial place (TPU) is used to bury the dead. There is an active decomposition of corpses in the soil, which produces soil nutrients and minerals that can support the growth of microorganisms in the ground, including pathogenic bacteria. Pathogenic bacteria have a more severe impact if they are resistant to antibiotics. Methods: Soil samples were taken in the Bonoloyo TPU area, Surakarta, Central Java, at 3 points each of 2 depths of 20 and 50 cm. Soil samples were inoculated on NA (Nutrient agar) media using the spread plate method. After 48 hours, colony counting and morphology observations were carried out, followed by gram staining. Isolated bacterial isolates were tested for resistance to 3 types of antibiotics. Results: The average population of soil bacteria in blocks 12, 17, and 21 at a depth of 20 cm is 4 x 10⁶ CFU/g; 8 x 10⁶ CFU/g; and 1 x 10⁶ CFU/g, while at a depth of 50 cm, it is 2.3 x 10⁶ CFU/g; 6 x 10⁶ CFU/g; and 4 x 10⁶ CFU/g. The morphology of bacterial colonies is irregular (26 isolates), flat elevation (19 isolates), and the color of isolates is predominantly white. The Gram staining results obtained 23 isolates are Gram-negative, and 22 are Gram-positive with a dominant cell form in cocci. Antibiotic resistance tests showed that the bacterial isolates were resistant to ampicillin (28.9%), Bacitracin (64.4%), and Cefepime (57.8%). Conclusions: Burial soil bacterial populations at Bonoloyo TPU at different depths did not differ markedly, with numbers ranging from 1 - 8 x 10⁶ CFU/g.
Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil Syarifah, Siti Nur; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11745

Abstract

Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
Test of Hypersensitivity and Antagonistic Reaction of Endophytic Bacteria from Klutuk Banana (Musa balbisiana) Dewianty, Ratih; Rahayu, Triastuti; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11795

Abstract

Background: Plants’ response and antagonistic reaction against disease-causing organisms are two crucial characteristics of Plant Growth Promoting Bacteria (PGPB). Seventeen endophytic bacteria have been isolated from Musa balbisiana. However, the plants’ reaction against these isolates and their antagonistic activities against disease-causing organisms remained unknown. This study aims to (1) determine the sensitivity effect of endophytic bacteria isolates to tobacco and (2) test the antagonism of endophytic bacteria isolates against the blast disease actor, namely Pyricularia oryzae. Method: Sensitivity tests were performed by inoculating bacterial isolates into tobacco leaves with infiltration. Bacterial isolates were prepared with a minimum OD 600: 0.5. Then, a milliliter volume of each isolate was infiltrated into tobacco leaves from the abaxial side using a 3 mL syringe. The lesion on the tobacco leaves was observed seven days after inoculation (DAI). The antagonism test was carried out by growing Pyricularia oryzae on a PDA plate for seven days, subsequently transferred to an NA medium for three days then inoculated with endophytic bacteria. The assessed parameter of the antagonistic test was the formation of an inhibitory zone between endophytic bacteria and Pyricularia oryzae at 4 DAI. Results: No hypersensitive reaction of tobacco leaves against K117, K324, K38, K86, K18, K28, K102 isolates inoculation at 7 DAI. Furthermore, the antagonistic test indicated that all isolates inhibited the growth of Pyricularia oryzae, with the range of inhibition from 32.36 to 40.46%. Implication: Thus, these results revealed the PGPB characteristics in the newly isolated endophytic bacteria from the banana.
Development of Electronic Module with Project based Learning Fadhilah, Nurul; Thahir, Rahmatia
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11817

Abstract

Background: Educators in the 21st century are faced with the demand to integrate technology into the learning process. One example is by using an electronic module (e-module). The electronic module is an innovative learning media presented digitally, including text, images, and video. This study aims to develop a valid and practical e-module based on project-based learning. Methods: The research and development (R&D) method was used in this study by adopting the Alessi & Trollip development model. Alessi & Trollip's model has three stages: planning, design, and development. Results: The results of e-module validation based on content experts show a value of 4.39 with a very valid category, and the percentage of validator assessment is 87.71%, which means that e-module is in the very feasible category. The media expert validator's assessment was 4.48, which means it is in the very valid category. The presentation of the validator's assessment was 89.58%, meaning that the e-module is suitable for use in the learning process. Student response to e-module based on project-based learning was 4.35 and categorized as very good. The percentage of student interest in the e-module is 86.90%. This value indicates that the e-module based on project-based learning meets the criteria of practicality and is feasible for students to use in the learning process. Conclusions: The e-module based on project-based learning developed in this study shows valid validity and practicality, So the e-module is appropriate for use in the learning process. The use of e-modules based on project-based learning also has the potential to increase students' critical thinking skills.
Pengaruh Proyek Zero waste Terintegrasi ESD Terhadap Kompetensi Kolaborasi Dan Self-awareness pada konsep Perubahan Iklim Damayanti, Teti; Suhendar, Suhendar; Juhanda, Aa
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11882

Abstract

Background: Low collaboration competence and Self-awareness provided to students to face sustainable development because collaboration competence and Self-awareness have not been developed in learning activities, especially to overcome and face global issues in the 21st century. This study aims to apply learning with Project Based Learning to determine the effect on collaboration competence and self-awareness. This model is very rarely used because it requires careful preparation, Methods: The type of research used in this study is quasi-experimental research. The research design in this study used a Non-equivalent Control Group Design. In this design, the determination of the experimental class and control class is not randomly selected. Results: In collaboration and self-awareness competence, the test results obtained a sig value. 2-tailed 0.000 which indicates that there is a significant difference. A correlation test was carried out on Self-awareness competence where the test results between the two obtained a value of 0.596 which was in the medium category. This figure shows that collaboration competencies with Self-awareness competencies have a correlation and show a fairly strong relationship. Conclusion: The implementation of learning by applying an integrated project learning model ESD to the concept of climate change, states that project learning affects collaboration competence and self-awareness with a significance value from the results of the static independent sample T-test 0.000. In addition to these two competencies can be improved through project learning. Collaboration competence and self-awareness competence are positively correlated. With a correlation value of 0.596 belongs to the medium category.
The Effect of Lamtoro Leaf POC and Tofu Liquid Waste on the Growth of Cayenne Chili (Capsicum frutescens) Andriyani, Siska; Suparti, Suparti
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11885

Abstract

Background: Liquid organic fertilizer is a fermented solution of organic plant residues, animal and human feces containing nutrients. Lamtoro and tofu liquid waste contain N, P, K, Mg, and Ca, which are suspected to have the potential for liquid organic fertilizer to increase the growth of cayenne pepper (C. frutescens). This study aims to determine the effect of giving POC from lamtoro leaves and tofu liquid waste on the growth of cayenne pepper (C. frutescens). Methods: This study used a completely randomized design (CRD) with two replications. POC volume 1:1 consisted of two-liter lamtoro leaf extract and 2-liter tofu liquid waste. The first factor is the volume concentration of POC with the respective concentrations of V0: 0%, V1: 10%, V2: 20%, and V3: 30%. The second factor is the time of spraying POC T1: 3 days interval and T2: 6 days interval. Parameters measured included plant height (cm), number of leaves (strands), and plant fresh weight (g). Results: 20% volume (V2) with three-day intervals (T1) gave the best results for plant height (20.10 cm), number of leaves (7.00 leaves), and plant fresh weight (11.50 g). 0% volume (V0) with an interval of 6 days (T2) gave the lowest yield on plant height (6.35 cm), number of leaves (3.00 leaves), and fresh weight of plants (3.00 leaves). Conclusions: Application POC from lamtoro leaves and tofu liquid waste can potentially increase the growth of cayenne pepper (C. frutescens).
Formulasi Gel Ekstrak Arang Tempurung Kelapa dan Uji Sensitivitas Terhadap Bakteri Escherichia coli Yogaswari, Dinya; Kurniawan, Kurniawan; Mujahid, Ikhsan; Ritma Dhanti, Kurnia
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11906

Abstract

Background: Escherichia coli bacteria are normal flora bacteria in the human digestive tract that can turn into opportunistic pathogens and cause diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). The existence of this bacterial infection is generally treated with the use of antibiotics, which have been shown to cause resistance to the E. coli bacteria, so it is necessary to treat them using other methods, namely by using drugs derived from natural ingredients (herbal medicines) that contain antibacterial compounds such as charcoal extract. coconut shell. The purpose of this study was to obtain the most effective coconut shell charcoal extract gel formulation for inhibiting the growth of E. coli bacteria in vitro. Methods: The method used in this study was a true experimental post-test only control group with treatment at different gel concentrations of 3%, 6%, and 9% with repetition. Results: The results of the one-way ANOVA analysis test showed that the treatment given to several concentrations of coconut shell charcoal extract had a mean difference in the area of the inhibition zone for E. coli bacteria with a known significance value of 0.002 (p <0.05), so it was continued with the post-host LSD test, and the results of several groups show a significant value (p <0.05) marked with the notation (*). Conclusion: Based on the results of the study, it can be concluded that the gel formulation of coconut shell charcoal extract with a concentration of 3% is most effective in inhibiting the growth of E. coli bacteria in vitro.

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