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Contact Name
Dessy Ariyanti
Contact Email
dessy.ariyanti@che.undip.ac.id
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+62247460058
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j.reaktor@che.undip.ac.id
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Department of Chemical Engineering, Diponegoro University Jl. Prof. Soedarto SH Tembalang Semarang 50275
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Jawa tengah
INDONESIA
Reaktor
Published by Universitas Diponegoro
Reaktor invites contributions of original and novel fundamental research. Reaktor publishes scientific study/ research papers, industrial problem solving related to Chemical Engineering field as well as review papers. The journal presents paper dealing with the topic related to Chemical Engineering including: Transport Phenomena and Chemical Engineering Operating Unit Chemical Reaction Technique, Chemical Kinetics, and Catalysis Designing, Modeling, and Process Optimization Energy and Conversion Technology Thermodynamics Process System Engineering and products Particulate and emulsion technologies Membrane Technology Material Development Food Technology and Bioprocess Waste Treatment Technology
Articles 530 Documents
EKSTRAKSI KONTINYU DENGAN SIMULASI BATCH TIGA TAHAP ALIRAN LAWAN ARAH: PENGAMBILAN MINYAK BIJI ALPUKAT MENGGUNAKAN PELARUT N-HEXANE DAN ISO PROPIL ALKOHOL Bambang Pramudono; Septian Ardi Widioko; Wawan Rustyawan
Reaktor Volume 12, Nomor 1, Juni 2008
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.091 KB) | DOI: 10.14710/reaktor.12.1.37-41

Abstract

Tujuan penelitian ini adalah mengkaji rendemen dan selektivitas dari ekstraksi biji alpukat dengan pelarut n-hexane dan isopropyl alcohol (IPA). Ekstraksi dilakukan dengan sistem kontinyu simulasi batch bertahap tiga aliran lawan arah pada temperatur 82 oC (untuk pelarut n- hexane) dan 69 oC (untuk pelarut IPA), selama 2 jam dengan perbandingan bahan dan pelarut sebesar 20 gr/250 gr. Rendemen yang diperoleh pada penggunaan n-hexane dan IPA sebesar 18,69 % dan 17,87 %. Kedua minyak yang dihasilkan baik dari penggunaan n-hexane maupun IPA memenuhi spesifikasi minyak biji alpukat. Dari besarnya rendemen yang diperoleh menunjukkan bahwa secara kuantitatif  pelarut n-hexane lebih baik daripada IPA, tetapi dari segi kualitatif (kandungan impuritas) pelarut IPA lebih baik daripada n-hexane.
Recovery of Cu (II) and Cr (VI) Metals from Electroplating Liquid Waste Using Polymer Inclusion Membrane (PIM) muhammad cholid djunaidi; Nor Basid Adiwibawa Prasetya; Nur Asih Setyowati; Khabibi Khabibi; Khoirun Nita Ulfia
Reaktor Volume 18 No. 2 June 2018
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (643.189 KB) | DOI: 10.14710/reaktor.18.2.102-109

Abstract

Research on recovery of Cu (II) and Cr (VI) from electroplating liquid waste has been carried out using a Polymer Inclusion Membrane (PIM). PIM has a high stability to overcome the weakness of liquid membrane instability. The PIM membrane was placed between two phases namely the feed phase as a metal source of Cu (II) from electroplating waste with pH 3 and the receiving phase which is HNO3 solution with pH 1 and pH 4.4 in the feed phase and pH 6.3 in the receiving phase with NaCl 2 N for metal Cr (VI). Recovery efficiency is known by determining the concentration of Cu (II) and Cr (VI) in the feed and receiving phases using AAS, while the membrane characterization were carried out using FTIR, SEM and UV-vis spectroscopy. The resulting PIM membrane has properties of thin, clear and flexible. AAS data showed that the percentage of PIM transport with stirring for 24 hours performed the highest percentage of Cu (II) metal transport from feed phase of 83.41% and transport in the receiving phase of 72.56%. While the percentage of optimum Cr (VI) transport was 97.8% in the feed phase and 76.5% in the receiving phase. The results of membrane characterization using FTIR and UV spectroscopy showed that the PIM membrane was quite stable. While the results of SEM show that there were no pores in the membrane so that the transport occurs by diffusion through the intermediate of the carrier compound. Keywords: electroplating waste; liquid membrane; PIM
Biofiksasi CO2 Oleh Mikroalga Chlamydomonas sp dalam Photobioreaktor Tubular Hadiyanto Hadiyanto; W Widayat
Reaktor Volume 15, No.1, APRIL 2014
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.288 KB) | DOI: 10.14710/reaktor.15.1.37-42

Abstract

Mikroalga memiliki potensi dalam membiofiksasi CO2 dan dapat dimanfaatkan untuk mengurangi kadar CO2 dalam gas pencemar. Pertumbuhan mikroalga sangat dipengaruhi oleh konsentrasi gas CO2 di dalam gas pencemar. Tujuan penelitian ini adalah untuk mengeetahui kemampuan mikroalga Chlamydomonas sp yang dikultivasi dalam photobioreaktor tubular dalam penyerapan gas CO2 serta untuk mengetahui konsentrasi maksimum gas CO2 dalam umpan untuk memproduksi biomasa mikroalga yang optimal. Percobaan dilakukan dnegan memvariasi laju alir dari 0.03 -0.071 L/menit dan konsentrasi CO2 dalam umpan 10-30%. Hasil penelitian menunjukkan bahwa biomasa mikroalga dapat diproduksi dengan maksimal dengan konsentrasi gas CO2 20% dengan laju alir 0.07 L/min. Semakin tinggi laju alir maka produksi biomasa alga semakin besar. Kecepatan pertumbuhan alga maksimum terjadi pada 0.31 /hari. Pada konsentrasi gas CO2 30%, terjadi substrate inhibition yang disebabkan carbon dalam bentuk ion bicarbonate tidak dapat dikonsumsi lagi di dalam kultur alga. Kata kunci : Mikroalga, chlamydomonas sp, biofiksasi CO2, biogas Abstract Microalgae have a potential for CO2 biofixation and therefore can be used to reduce the CO2 concentration in the gas pollutants. Moreover, microalgae growth is strongly affected by the concentration of CO2 in the exhaust gas pollutants. The objective of this research was to investigate the ability of microalgae Chlamydomonas sp which was cultivated in a tubular photobioreactor for CO2 absorption as well as to determine the maximum concentration of CO2 in the feed gas to obtain optimum microalgae biomass. The experiments were performed by varying the gas flow rate of 0.03 -0.071 L / min and the concentration of CO2 in the feed of 10-30%. The results showed that the maximum biomass of microalgae can be produced with CO2 concentration of 20% vol with a flow rate of 0.07 L / min. The result also showed that increasing the gas flow rate, the greater of the production of algal biomass and the maximum algae growth rate occurred at 0.31 / day. At a concentration of 30% CO2 gas, it occurs a substrate inhibition due to inefficient of bicarbonate use by algae culture.
Two Dimentional Numerical Models Of Hollow Fiber Membrane Contactor N. Aryanti; Y. Bindar; I. G. Wenten
Reaktor Volume 6 No. 2 Desember 2002
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6292.075 KB) | DOI: 10.14710/reaktor.6.2.77-84

Abstract

Membrane contactor is separation processing unit using membrane as a contacting device. The major advantage of membrane contactor relies on its high contact area compared to conventional scrubber. One of the important applications of membrane contactor is to reduce emission of acid gases. In this work, modeling of membrane contactor is conductedto describe concentration distribution along fiber length used to predict effective fiber length by solving mass conservation equation. Solving of mass conservation equation required information of fluid flow  distribution obtained by solving continuity and momentum equation simultaneously. The finite volume method is used to obtain the solution. Modeling of fluid flow was carried out by adding Darcy`s and Brinkman-Darcy flow models into Navier-Stokes equation. The momentum and continuity equation  are solved for two-dimentional cylindrical coordinate. The result of velocity profile at axial direction were validated with Pangrle et.al. (1992) experimental data. The comparison shows that consideration using Brinkman-Darcy flow model give agood agreement with experimental data in which maximal axial velocity achieved is 0,047 m/s for this model and 0,05 m/s for experimental data.the concentration profile at radial direction using Darcy and Brickman-Darcy flow models have also been investigated. Furthermore, concentration profile at axial direction using the both two flow models indicate a decrease of concentration along fiber length. The comparison between models and experimental data by Subhakti and Azmier (1997) agree very closely to the Brinkman- Darcy flow model. The prediction of effective  fiber length was conducted based on minimum economical flux oe\f membrane contactor. The calculation gives the effective fiber length obtained is 0.19 m at gas concentration, gas flow rate, and sorbent concentration of 0.02 mol/L, 0.8 m/s and 0.256 M respectively.Keywords : modeling, membrane contactor, Darcy, Brinkman-Darcy
MEMBRANE BIOREACTOR FOR TREATMENT OF RECALCITRANT WASTEWATERS Suprihatin Suprihatin; Muhammad Romli
Reaktor Volume 13, Nomor 1, Juni 2010
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (439.235 KB) | DOI: 10.14710/reaktor.13.1.1-9

Abstract

The low biodegradable wastewaters remain a challenge in wastewater treatment technology. The performance of membrane bioreactor systems with submerged hollow fiber micro- and ultrafiltration membrane modules were examined for purifying recalcitrant wastewaters of leachate of a municipal solid waste open dumping site and effluent of pulp and paper mill. The use of MF and UF membrane bioreactor systems showed an efficient treatment for both types wastewaters with COD reduction of 80-90%. The membrane process achieved the desirable effects of maintaining reasonably high biomass concentration and long sludge retention time, while producing a colloid or particle free effluent. For pulp and paper mill effluent a specific sludge production of 0.11 kg MLSS/kg COD removed was achieved. A permeate flux of about 5 L/m²h could be achieved with the submerged microfiltration membrane. Experiments using ultrafiltration membrane produced relatively low permeate fluxes of 2 L/m²h. By applying periodical backwash, the flux could be improved significantly. It was indicated that the particle or colloid deposition on membrane surface was suppressed by backwash, but reformation of deposit was not effectively be prevented by shear-rate effect of aeration. Particle and colloid started to accumulate soon after backwash. Construction of membrane module and operation mode played a critical role in achieving the effectiveness of aeration in minimizing deposit formation on the membrane surface.
The Impact of Combination of Ozonation and Ultrasonication Process on Morphological and Chemical Properties of κ-Carrageenan Nurfiningsih Nurfiningsih; Ratnawati Ratnawati; Aji Prasetyaningrum
Reaktor Volume 19 No. 2 June 2019
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.533 KB) | DOI: 10.14710/reaktor.19.2.49-53

Abstract

Carrageenan application in the biomedical field is influenced by the size of molecular weight. Depolymerization process is needed for changing the high molecular weight (HMW) fraction of κ- carrageenan into low molecular weight fraction (LMW) of κ- carrageenan. Ozone is a powerful oxidant and considered for depolymerization of κ- carrageenan. This research was conducted as an investigation on the influence of the combination of ozonation and ultrasonication (OZ/US) on the chemical and morphological characteristics of κ-carrageenan. FTIR and Scanning electron microscopy (SEM) was used to determine the change of chemical and morphological characteristics of κ-carrageenan. The result of this study showed that there was no change of chemical properties of κ-carrageenan during OZ/US treatment. The sulfate content was obtained after OZ/US treatment tends to be stable.  Nevertheless, the result of SEM analysis showed that there were changes on morphological characteristics of κ-carrageenan after OZ/US treatment.  Keywords: ultrasonication, κ-carrageenan, ozonation, morphological, chemical
Karakterisasi Pengemas Kertas Aktif dengan Penambahan Oleoresin dari Ampas Pengepresan Rimpang Temulawak (Curcuma xanthorrhiza Roxb) Windi Atmaka; Godras Jati Manuhara; Noreka Destiana; K Kawiji; Lia Umi Khasanah; Rohula Utami
Reaktor Volume 16 No.1 Maret 2016
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.644 KB) | DOI: 10.14710/reaktor.16.1.32-40

Abstract

CHARACTERIZATION OF ACTIVE PAPER PACKAGING INCOORPORATED WITH OLEORESIN EXTRACTED FROMSOLID WASTE OF PRESSED Curcuma xanthorrhiza Roxb ROOT. Curcuma xanthorrhiza Roxb is the medicinal plant that is widely extracted for instant drinks by herbal industry. In producing this instant Curcuma xanthorrhiza Roxb, solid waste is also produced and then thrown away. The waste actually still contain oleoresin as an active material. In this study, active paper packaging were incorporated with oleoresin extraxted from the waste at 0, 2, 4, and 6% (w/w) level. The aims of this study was to determine the effect of oleoresin concentration on physical, chemical, and antimicrobial activity characteristics of active paper packaging. Based on this research, the incorporation of the oleoresin decreased moisture content, tensile strength, folding endurance and but increased antimicrobial activity of Aspergillus niger.   Keywords: active paper packaging; curcuma xanthorrhiza Roxb.; oleoresin; residue Abstrak Temulawak (Curcuma xanthorrhiza Roxb) adalah tanaman herbal yang lazim diolah industri herbal menjadi minuman instan. Produksi minuman instan temulawak menghasilkan limbah padat berupa ampas yang tidak dimanfaatkan dan biasanya hanya dibuang begitu saja. Padahal ampas temulawak masih mengandung oleoresin yang berperan sebagai senyawa aktif. Penelitian kertas aktif dilakukan dengan menambahkan oleoresin ampas temulawak dengan konsentrasi 0, 2, 4, dan 6% (b/b). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan oleoresin ampas temulawak terhadap karakter fisikokimia, aktivitas antimikroba dan sensoris pengemas kertas aktif. Dari penelitian ini menunjukkan bahwa penambahan oleoresin ampas temulawak menurunkan kadar air, nilai ketahanan tarik, ketahanan lipat, dan karakter sensori (warna, tekstur dan overall), namun meningkatkan aktivitas antimikroba terhadap Aspergillus niger. Kata kunci: pengemas kertas aktif; temulawak; oleoresin; ampas  
Pemetaan Pengaruh Proses Pengolahan pada Kualitas Biji Kakao Menggunakan Metode Metabolik Profiling dengan GC/MS Made Tri Ari Penia Kresnowati; Hana Nur Fitriana; Ronny Purwadi
Reaktor Volume 17 No. 3 September 2017
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.458 KB) | DOI: 10.14710/reaktor.17.3.132-139

Abstract

Abstract MAPPING THE EFFECTS OF COCOA POST HARVEST PROCESSING ON COCOA BEAN QUALITY BY METABOLIC PROFILING METHOD USING GC/MS. The quality of cocoa is strongly effected by the implemented post harvest processes. The fermented cocoa beans is presumed to have higher quality than the unfermented ones, whereas roasting strongly affects the organoleptic quality of the cocoa products. However, what exactly differentiates those cocoa beans are not known yet. This research was foccused on the mapping of post harvest processing method on the quality of cocoa beans, using metabolic profiling method. Different types of cocoa bean samples, that were fermented, roasted, fermented and roasted, as well as unfermented and unroasted (as control) were analysed by GC-MS. The obtained metabolic profiles were further mapped using Principal Component Analysis (PCA). The results showed that there were significant different on samples processed through different processing methods. The fermentation and roasting of cocoa beans direct the process such that more homogenous metabolic composition were obtained. Kata kunci: cocoa beans; fermentation; metabolomic; PCA; processing; roasting  Abstract Proses pengolahan biji kakao sangat menentukan kualitas kakao yang dihasilkan. Biji kakao terfermentasi diyakini memiliki kualitas yang lebih tinggi dibandingkan biji kakao tanpa fermentasi. Proses penyangraian juga diyakini sangat menentukan kualitas organoleptik produk olahan kakao. Akan tetapi, para pelaku bisnis kakao masih belum mengetahui secara pasti apa yang membedakan hal tersebut. Penelitian ini berfokus pada pemetaan pengaruh proses pengolahan pada kualitas biji kakao, menggunakan metode metabolik profiling. Sampel biji kakao dengan berbagai proses pengolahan: difermentasi, disangrai, difermentasi dan disangrai, serta kontrol (tidak difermentasi dan tidak disangrai) dianalisa dengan GC/MS untuk memetakan komposisi metabolitnya. Spektrum metabolit yang diperoleh kemudian dipetakan menggunakan metode Principal Component Analysis (PCA). Hasil yang diperoleh menunjukkan terdapat perbedaan yang signifikan antara sampel dengan berbagai proses pengolahan tersebut, pengolahan biji kakao yang terdiri dari fermentasi dan penyangraian mengakibatkan komposisi biji kakao yang lebih seragam. Keywords: biji kakao; fermentasi; metabolomik; PCA; pemrosesan; penyangraian
Ekstraksi Minyak Nilam Dengan Pelarut N-Heksana B. Jos
Reaktor Volume 08 No.2 Desember 2004
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4384.329 KB) | DOI: 10.14710/reaktor.8.2.94-99

Abstract

Minyak nilam atau “patchouli oil” merupakan komoditas ekspor yang memberikan sumbangan devisa paling besar  diantara minyak atsiri yang lain di Indonesia. Minyak nilam digunakan sebagai bahan pewangi dan penahan (bersifat fiksatif) aroma wangi dalam pembuatan parfum, kosmetika, sabun, minyak rambut dan saus tembakau. Pengambilan minyak nilam ini umumnya melalui proses penyulingan dengan air maupun dengan distilasi uap. Pada proses ini rendeman yang dihasilkan hanya sekitar 2- 2,5% dengan konsentrasi patchouli alcohol (PA) sebesar 31%. Oleh karena itu upaya untuk meningkatkan mutu dan rendemen minyak nilam perlu dilakukan. Salah satu metode yang digunakan adalah dengan cara ekstraksi-destilasi. Proses ekstraksi dilakukan dengan menggunakan pelarut n-heksana dan variabel yang digunakan yaitu  waktu ekstraksi (30, 60, 90, 120, 150, 180, 210 menit), volume pelarut (2000, 2500, 3000, 3500, 4000, 4500, 5000 ml), serta suhu ekstraksi (30, 40, 50, 60 0C) dengan berat daun nilam yang digunakan sebesar 200 gram, kemudian produk keluaran ekstraksi diteruskan dengan proses distilasi (tahap pemurnian). Hasil penelitian menunjukkan bahwa kondisi operasi optimumdiperoleh pada waktu ekstraksi 120 menit, volume pelarut 3000 ml, dan suhu ekstraksi 30 0C (suhu kamar). Rendemen yang diperoleh sebesar 4,51% dengan konsentrasi patchouli alcohol (PA) sebesar 37%. Ekstraksi-destilasi dengan pelarut n-heksana ini, menghasilkan mutu dan rendemen minyak nilam yang lebih baik daripada proses penyulingan dengan air maupun destilasi uap biasa.Kata kunci : Patchouli alcohol, Rendemen, minyak nilam
PERFORMA OKSIDASI METAN PADA REAKTOR KONTINYU DENGAN PENINGKATAN KETEBALAN LAPISAN BIOCOVER LANDFILL Opy Kurniasari; Tri Padmi; Edwan Kardena; Enri Damanhuri
Reaktor Volume 14, No. 3, APRIL 2013
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.05 KB) | DOI: 10.14710/reaktor.14.3.179-186

Abstract

PERFORMANCE OF METHANE OXIDATION IN CONTINUOUS REACTOR BY BIOCOVER LANDFILL FILM THICKNESS IMPROVEMENT. Municipal solid waste (MSW) handling in Indonesia is currently highly dependent on landfilling at the final disposal facility (TPA), which generally operated in layer-by-layer basis, allowing the anaerobic (absent of oxygen) process. This condition will certainly generate biogas in the form of methane (CH4) and CO2. Methane is a greenhouse gas with a global warming potential greater than CO2, and can absorb infrared radiation 23 times more efficient than CO2 in the period of over 100 years. One way that can be done to reduce methane gas from landfills that escape into nature is to oxidize methane by utilizing landfill cover material (biocover) as methane-oxidizing microorganism media. Application of compost as landfill cover material is a low-cost approach to reduce emissions so are suitable for developing countries. The compost used in this study was compost landfill mining, which is degraded naturally in landfill. The purpose of this study was to evaluate the ability of biocover to oxidize the methane on a certain layer thickness with a continuous flow conditions. Three column reactors were used, which were made of flexy glass measuring 70 cm in high and 15 cm in diameter. The methane flowed from the bottom of the reactor continuously at a flow rate of 5 ml/minute. The columns were filled with biocover compost landfill mining with layer thickness of 5, 25, 35 and 60 cm. The results showed that the thicker layer of biocover, the higher the efficiency of methane oxidation. The oxidation efficiency obtained in each layer thickness of 15, 25, 35 and 60 cm was 56.43%, 63.69%, 74.58% and 80, 03% respectively, with the rate of oxidation of 0.29 mol m-2 d-1 and the fraction of oxidation of 99%. The oxidation result was supported by the identification of bacteria isolated in this experiment, namely metanotrophic bacteria that have the ability to oxidize methane through the form of methanol metabolite. ABSTRAKPenanganan sampah kota di Indonesia pada umumnya dilakukan pada tempat pemrosesan akhir sampah (TPA), yang sebagian besar dilakukan dengan cara pengurugan (landfilling) yang cenderung bersifat anaerob (tidak ada oksigen). Cara pengurugan ini biasanya dioperasikan lapis perlapis sehingga memungkinkan terjadinya proses anaerob. Pada kondisi ini dipastikan biogas, yaitu gas metana (CH4) dan CO2, akan muncul. Metana adalah gas rumah kaca dengan potensi pemanasan global lebih besar dari CO2, dan dapat mengabsorpsi radiasi infra merah 23 kali lebih efisien dari CO2 pada periode lebih dari 100 tahun. Salah satu cara yang dapat dilakukan untuk mengurangi gas metana dari landfill yang lepas ke alam adalah dengan mengoksidasinya dengan memanfaatkan material penutup landfill (biocover) sebagai media mikroorganisma pengoksidasi metana. Aplikasi kompos sebagai material penutup landfill merupakan pendekatan dengan biaya rendah untuk mereduksi emisi gas dari landfill sehingga cocok untuk negara berkembang. Biocover yang digunakan pada penelitian ini adalah kompos landfill mining, yaitu kompos yang terdegradasi secara alami di landfill. Tujuan penelitian ini adalah mengevaluasi kemampuan biocover kompos landfill mining dalam mengoksidasi metana pada ketebalan lapisan tertentu dengan kondisi aliran kontinyu. Tiga buah reaktor kolom yang digunakan terbuat dari flexy glass berukuran tinggi 70 cm dan diameter 15 cm. Gas metana dialirkan dari bawah reaktor secara kontinyu dengan laju alir 5 ml/menit. Kolom diisi dengan biocover kompos landfill mining dengan ketebalan lapisan 5, 25, 35 dan 60 cm. Hasil percobaan menunjukkan bahwa semakin tebal lapisan biocover, semakin tinggi efisiensi oksidasi metana. Efisiensi oksidasi yang diperoleh pada setiap ketebalan lapisan 15, 25, 35 dan 60 cm adalah masing-masing 56,43%, 63,69%, 74,58% dan 80,03%, dengan laju oksidasi 0,287 mol m-2 d-1 dan fraksi oksidasi 97%. Hasil oksidasi yang diperoleh tersebut diperkuat  dengan identifikasi bakteri yang berhasil diisolasi, yaitu bakteri metanotrofik yang memiliki kemampuan dalam mengoksidasi metana melalui metabolit antara berupa metanol.  

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