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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD Saputro, Triono Bagus; Finariyah, Fathin; Dianawati, Siti; Sholihah, Nur Fadlilatus; Ermavitalini, Dini
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6934

Abstract

Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the needs were not meet to the demands since conversion of agricultural area increase lately. Indonesia has 99.093 km of shoreline that recognize as marginal land where the salinity is high as well. This research try to obtain tolerant variant from two local cultivars that planted in Madura Island. Manding and Talango varieties were used as an explant for callus induction stage in MS supplemented with 2.4 D. The result show that 4 ppm of 2.4 D were the best concentration to induce the callus in both varieties. The induced callus were exposed to medium MS that contain NaCl (0, 2500, 5000, and 7500 ppm). In 7500 ppm of NaCl, Manding variety has 100% of surviving callus, while Talango variety only 66.7%. Furthermore, Manding variety shows a better performance in callus weight improvement with 170 mg, while Talango shows no improvement of callus weight. The result of RAPD analysis indicate that the genome characteristic were different between initial callus and surviving callus. Out of ten primers, only five primers shows polymorphism i.e OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19. Manding can be further developed as high tolerance variety in Salinity stress, thus it proposed to be developed furthermore.How to CiteSaputro, T. B., Finariyah, F., Dianawati, S., Sholihah, N. F., & Ermavitalini, D. (2016). In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD. Biosaintifika: Journal of Biology & Biology Education, 8(3), 344-352.
Colour and Morphometric Variation of Donacid Bivalves from Nepa Beach, Madura Island, Indonesia Ambarwati, Reni; Faizah, Ulfi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11267

Abstract

Donacid bivalves vary in colour, pattern, and the shape of the shells. A population of donacid bivalves had been found in the northern shore of Madura Island, Indonesia. This study aimed to identify the donacids found in northern shore of Madura Island as well as to describe their variation on morphometric and morphological characters. Colour and pattern of 215 shells were observed and carefully photographed. Morphometric of the shells including the shell height, shell length, shell width, dorsal length, and umbo-margin length were measured and then their patterns were analyzed using regression analysis. The results revealed that there were two species of donacid bivalves in Nepa Beach, namely Donax cuneata and Donax faba with different morphological characteristics, including twelve different patterns and colours of the shells. These variations were described completely in this paper. This study contributes to the research on bivalve taxonomy, and is useful for the identification of donacid bivalves based on morphological characteristics.
An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.) Roslim, Dewi Indriyani; Nisa, Fitriyatun; Herman, Herman
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5365

Abstract

Sweet and bitter taste on cassava tuber is affected by starch metabolisms. Meisa1 gene is a gene in cassava (Manihot esculenta Crantz.) encoding isoamylase1 enzyme involved in starch metabolisms. This study aimed to analyze partial DNA sequences of Meisa1 gene on sweet and bitter cassavas collected by Genetics Laboratory, Department Biology, Faculty of Mathematics and Natural Sciences, Riau University, Indonesia. Methods included total DNA extraction from fresh young leaves of cassava using CTAB buffer, polymerase chain reaction (PCR), electrophoresis, and sequencing. The obtained data were analyzed using MEGA software version 5. The results showed that there were nucleotide variations in the intron region, not in the exon region. The variations were caused by the transition substitution mutation (35.39%) and transversion substitution mutation (64.61%). The genetic distance range between seven cassava genotypes was approximately 0% to 11%. Partial DNA sequence variations of Meisa1 gene located in intron region were unable to cluster seven cassava genotypes separately into two groups based on tuber taste.How to CiteRoslim, D., Nisa, F., & Herman, H. (2016). An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.). Biosaintifika: Journal of Biology & Biology Education, 8(1) 103-110.
Identifikasi dan Kelimpahan Lalat Buah Bactrocera pada Berbagai Buah Terserang Rini Indriyanti, Dyah; Nur Isnaini, Yanuarti; Priyono, Bambang
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2933

Abstract

Penelitian bertujuan mengidentifikasi spesies dan kelimpahan Bactrocera yang menyerang berbagai buah di Kecamtan Demak dan Dempet Kabupaten Demak. Penelitian menggunakan metode purposive sampling. Penelitian dilakukan dengan mengambil 5 macam buah yang terserang (jambu air, belimbing, jambu biji, melinjo dan mangga), pengambilan data faktor klimatik dilakukan pada saat pengambilan sampel. Buah terserang kemudian dilakukan rearing, Bactrocera spp yang didapat dilakukan identifikasi. Bactrocera yang menyerang jambu air yaitu Bactrocera ablistrigta, Bactrocera carambolae menyerang belimbing dan jambu biji, Bactrocera mcgregori menyerang melinjo dan Bactrocera papayae menyerang mangga. Kelimpahan Bactrocera albistrigata sebesar 112 individu/kg jambu air, Bactrocera carambolae pada belimbing sebesar 368 individu/kg belimbing, Bactrocera carambolae pada jambu biji sebesar 86 individu/kg jambu biji, Bactrocera papayae sebesar 48/kg mangga dan Bactrocera mcgregogi sebesar 668 individu/kg melinjo. Kelimpahan tertinggi terdapat pada Bactrocera mcgregogi yaitu 668 individu/kg melinjo. Kelimpahan terendah pada Bactrocera papayae yaitu 48 individu/kg mangga.This study was conduted in Demak and Dempet districts Demak regency. Demak is one of the producer of horticultural crops such as fruits. The objective of this research is identifying the species and abundance of Bactrocera which attacked various fruits in Demak regency. The research used was purposive sampling method. The steps of this research include 5 various infected fruits (water aple (, starfruit, guava, gnetum gnemon and mango climatic factors data collection is done at the time of sampling, developing fruit then carried rearing, Bactrocera spp obtained to identify. Bactrocera that infected the water aple (Bactrocera ablistrigta), Bactrocera carambolae attack starfruit and guava, Bactrocera mcgregori attack melinjo (Gnetum gnemon) and Bactrocera papayae attack mango. Bactrocera albistrigata abundance of 112 individuals/kg water aple, starfruit Bactrocera carambolae on by 368 individual/kg starfruit, guava Bactrocera carambolae on by 172 individual/kg guava, Bactrocera papayae by 48/kg mango and Bactrocera mcgregori of 668 individual/kg Gnetum gnemon.The highest abundance showed by Bactrocera mcgregori that is 668 individual/kg melinjo(Gnetum gnemon). The lowest abundance was (Bactrocera papayae 48 individual/ 0,5 kg mango).
Application of rps16 Intron and trnL-trnF Intergenic Spacer Sequences to Identify Rengas Clone Riau Roslim, Dewi Indriyani; Herman, Herman
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9108

Abstract

Rengas clone Riau has been identified using morphological characters and molecular technique with a psbA-trnH intergenic spacer, however, this method can only determine its taxonomic status at genus level, namely Gluta sp. This study reports application two DNA barcodes, i.e. rps16 intron and trnL-trnF intergenic spacer, to identify Rengas clone Riau. The methods included collection of the leaves from Kajuik Lake, total DNA isolation, electrophoresis, PCR (polymerase chain reaction), gel purification and sequencing. The rps16 intron size was 659 bp and the trnL-trnF intergenic spacer was 527 bp. The BLASTn analysis showed that sequences of the rps16 intron and the trnL-trnF intergenic spacer of Gluta sp clone Riau had 100% similarity to those of G. renghas deposited in GenBank. These results were supported by high max score, high total score, query cover = 100%, and E-value = 0. The dendrograms also showed the closest relationship of Gluta sp clone Riau with G. renghas deposited in GenBank compared to other species of Gluta. In conclusion, this study succeeded in identifying Rengas clone Riau as Gluta renghas by using sequences of the rps16 intron and the trnL-trnF intergenic spacer. A combination of DNA barcodes could be applied to identify various plants as long as the database for the DNA barcodes is available in public database such as GenBank.
Isolation of Housekeeping Genes on Durik-durik (Syzygium sp) Roslim, Dewi Indriyani; Yusfi, Liza Aulia; Hutagalung, Desriani Ritawati; Faizah, Niswah; Andriani, Lestari; Azrial, Azrial; Herman, Herman; Lestari, Wahyu
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14234

Abstract

Housekeeping gene is a gene expressed with a fixed level and in abundant amounts under various conditions. After validation, the housekeeping gene can be used as an internal control to normalize gene expression data. This study reports the isolation of several housekeeping genes in Durik-durik plant (Syzygium sp). This plant material in form of fresh leaves from Durik-durik plants are taken from Kajuik Lake, Riau Province. The next stage is total DNA isolation, polymerase chain reaction, electrophoresis, sequencing and data analysis using bioinformatic tools. The isolated housekeeping genes included 18S rRNA, actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Beta-tubulin and Ubiquitin with sizes of 400 bp, 679 bp, 1134 bp, 836 bp, 1167 bp and 2155 bp, respectively. In addition to 18S rRNA, the five housekeeping genes are the first reported from the genus Syzygium and referable to isolate housekeeping genes in other species in this genus. The six housekeeping genes can be used as internal controls on Durik-durik plants after validation.
REGULASI KORTISOL PADA KONDISI STRES DAN ADDICTION -, Lisdiana
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2264

Abstract

Stres adalah suatu kondisi dimanan tuntutan yang harus dipenuhi melebihi kemampuan yang dimilikinya, penyebab stres dinamakan stresor. Stres dapat terjadi akibat ketidakmampuan seseorang dalam merespon suatu stresor, sehingga dapat mengakibatkan gangguan badan atau jiwa. Addiction adalah suatu dorongan yang kuat, seperti dipaksakan untuk mengulangi suatu perbuatan tertentu meskipun tahu akan berakibat merugikan. Stress dan adicction akibat penyalahgunaan narkotika akan direspon oleh Hipotlamus-Pituitary-Adrenalin (HPA-axis), sehingga menye-babkan kadar hormon kortisol akan meningkat. Desain penelitian adalah Quasi-Eksperimental dengan Randomized Control Pretest-Postest Design Dengan subyek penelitian 22 Addict recovery yang memenuhi kriteria inklusi dan eksklusi di Balai Kasih Sayang Pamardisiwi BNN Jakata.Variabel yang diukur adalah hormone yang disekresikan oleh HPA-axis, yakni hormon kortisol. Pemeriksaan kadar kortisol dengan menggunakan Radioimmunoassay (RIA). Hasil penelitian menunjukkan kadar kortisol pada Addict recovery yang menjalani rehabilitasi sebesar 9,2 – 13,97 µg/dl dan 16,5-16,9 µg/dl pada Addict recovery yang tidak menjalani rehabilitasi. Hal ini dapat disimpulkan bahwa terjadi perubahan hormone yang disekresikan HPA-axis pada kondisi stress dan addiction. Stress is a condition where the demands to be met is beyond the capabilities of a person, and something that causes a stress is called stressor. Stress can occur as a result of the inability of a person in responding a stressor, and the stress can cause physical or mental disorders. Addiction is a strong drive, forced to repeat a particular action even it is known that it will harm the body. Stress and adicction to drug abuse will be responded by the hypothalamus-pituitary-adrenalin (HPA-axis), causing the levels of the hormone cortisol to rise. The study design was a randomized Quasi-Experimental Control Pretest-posttest design with 22 addict recovery study subjects who meet the inclusion and exclusion criteria in the National Narcotics Agency Jakarta.The variabel to be measured was cortisol secreted by the HPA-axis. The examination of the cortisol levels was using a radioimmunoassay (RIA). The results showed that the cortisol levels in the addict recovery subjects with and without rehabilitation were 9.2 to 13.97 mg / dl and 16.5 to 16.9 mg / dl, respectively. It was concluded that the levels of cortisol, secreted by HPA-axis in conditions of stress and addiction, have lowered.
Callus Induction of Gendarussa (Justicia gendarussa) by Various Concentration of 2,4-D, IBA, and BAP Wahyuni, Dwi Kusuma; Andriani, Putri; Ansori, Arif Nur Muhammad; Utami, Edy Setiti Wida
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11347

Abstract

Justicia gendarussa Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (? = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. Moreover, the optimum treatment will be used to produce secondary metabolite and seed synthetic by cell suspension culture.
Salinity Tolerance of Mungbean Genotypes at Seedling Stage Hapsari, Ratri Tri; Trustinah, Trustinah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13999

Abstract

Salinity is a major abiotic stress limiting mungbean production worldwide including Indonesia. Since mungbean plant is very sensitive to salt condition, selection of salinity tolerant genotypes becomes important for mungbean improvement. The objective of this study was to evaluate the tolerance of eight mungbean genotypes to salinity at seedling stage under different levels. The experiment was arranged in a randomized complete block design with two factors (mungbean genotypes and salinity levels) and triplicates. Observation variables were germination percentage, vigor index, germination rate, hypocotyls length, epicotyls length, root length, number of root, seedling fresh weight, and seedling dry weight. The result showed that increasing level of salinity concentration inhibited the speed of germination, germination percentage, vigor index, normal seedling fresh weight, and number of lateral roots. Murai and Vima 1 were identified as tolerant genotypes, while Vima-2 and MLGV 0180 were identified as salinity sensitive genotypes at seedling stage. Currently, mungbean varieties with special characters, such as saline-tolerant is not yet available. The availability of saline-tolerant variety of mungbean is a cheaper and easier technology for farmers to anticipate the expansion of the saline area. The tolerant genotypes may be further tested at the later stage to obtain promising genotype tolerant to salinity that effectively assist mungbean breeding program.
Knowledge Understanding and Utilization of Medicinal Plants by Local Community Tompu District of Kaili, Sigi Biromaru, Central Sulawesi Ifandi, Slamet; Jumari, Jumari; Suedy, Sri Widodo Agung
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4529

Abstract

Kaili is one of the ethnic region in Central Sulawesi which saves a lot of cultural values and traditions. As a traditional community, their life is very dependent upon natural resources contained in the environment. They still have knowledge, traditional culture, treatment and utilization system against various types of plants. The purpose of the study was to examine the knowledge understanding and utilization of medicinal plants by local community Tompu District of Kaili. Data knowledge and utilization were collected through interview, literature study, exploratory survey methods, PEA (participatory ethnobotanical appraisal), questionnaire and from interviews with the informants. The results from interviews showed that of public knowledge is still based on the traditional concept. Based on the results identifications obtained by (90 species). As many as six species medicinal plants to often used the Tompu community are Euphorbia hirta L. Phyllanthus niruri L. Ageratum L. Blumea conyzoides balsaminifera L. (DC). Kleinhovia hospita L and Tabernaemontana pandacaqui. The benefits of this research to the development of science is expected to be complete scientific data regarding the utilization of medicinal plants natural resources on Tribal society Kaili in Tompu.How to CiteIfandi, S., Jumari, J., & Suedy, S. W. A. (2016). Knowledge Understanding and Utilization of Medicinal Plants by Local Community Tompu District of Kaili, Sigi Biromaru, Central Sulawesi. Biosaintifika: Journal of Biology & Biology Education, 8(1), 1-11.

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