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Biosel: Biologi Science and Education

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Published by Institut Agama Islam Negeri Ambon

ISSN : 2252858X EISSN : 25411225 DOI : -

Core Subject : Science, Education, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science

Journal Biosel receiving articles of the results of the study in biology field and education. This journal rising twice a year, namely edition of january-june and edition of july-december.

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Articles 292 Documents

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Evaluasi Kondisi Ekosistem Lamun Thalassia hemprichii Melalui Transplantasi Metode Peat Pot Substrate Modification di Perairan Pantai Desa Suli Kabupaten Maluku Tengah Rosmawati, Rosmawati; Assagaf, Gamar
Biosel Biology Science and Education Vol. 14 No. 2 (2025): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v14i2.9326

Thalassia hemprichii is a type of seagrass that grows in the coastal waters of Suli Village, Central Maluku Regency and its distribution is quite wide, but its population is starting to decrease considering the damage to the seagrass, including due to the influence of waves and currents which cause the seagrass to be detached from its substrate or damage originating from the activities of the surrounding community such as coastal reclamation, waste pollution and "bameti" activities. Seagrass transplantation is one way to repair damaged seagrass beds. This study aims to analyze the survival rate, growth rate, biomass and physical and chemical factors of the waters that affect the growth of T. hemprichii seagrass. If so far the peat pot seagrass transplantation method uses polybags containing substrate from the donor area, this study tries to use substrate from the transplant area. The results showed that T. hemprichii had a survival rate of 100%. The average leaf growth rate in the 1st observation period was 0.145 cm/day, while in the 2nd observation period it was 0.172 cm/day. Seagrass leaf biomass ranges from 0.002 gbk/m2 –0.014 gbk/m2 with a total biomass value of 0.270 gbk/m2. Decree of the Minister of Environment Number 51 of 2004 concerning Sea Water Quality Standards, the value of the physical and chemical parameters of waters is still within the range of environmental parameters of marine waters that support the growth of T. hemprichii. Keywords: Transplantation, Peat Pot, Substrate, Modification Method

Inventarisasi Tanaman Obat di Desa Bontotangnga Kecamatan Bontolempangan Kabupaten Gowa Sulawesi Selatan T, Riska; Arifuddin, Widiastini; Liana, Alin
Biosel Biology Science and Education Vol. 14 No. 2 (2025): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v14i2.9336

Medicinal plants are an effective source of traditional medicine that has been used for generations to prevent and treat diseases. Inventory of medicinal plants needs to be done to collect and compile data on the types and potential of medicinal plants so that it can be used as guidelines in their utilization. This study aims to inventory the medicinal plants used in Bontotangnga Village, Bontolempangan District, Gowa Regency, South Sulawesi. This research is a descriptive survey study using qualitative approach. The data collection process was conducted through observation, and interviews. The technique of determining informants used purposive sampling. In the inventory process, data collection was carried out by collecting medicinal plant data including common names, scientific names, families, plant parts used, processing methods, and diseases treated. The results of the study showed that the types of medicinal plants used in Bontotangnga Village were 44 species from 22 families used to treat 35 types of diseases. Among the 22 families found, the Zingiberaceae family is the dominant medicinal plant used by the people of Bontotangnga Village to treat diseases. The most commonly used part of the plant is the leaves, which are usually prepared by boiling. Keywords: Inventory, Medicinal Plants, Bontotangnga Village

The English Halifah Pagarra; Rachmawaty; Musawira; Handayani, Besti; Syaiful Haq, Muhammad Naufal
Biosel Biology Science and Education Vol. 14 No. 2 (2025): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v14i2.9376

Matoa (Pometia pinnata) is a plant commonly used by the Indonesian people for traditional medicine due to its rich content of metabolites. This study aimed to analyze the phytochemical composition and antibacterial activity of matoa leaves The leaves were extracted using a 70% ethanol solvent with a ratio of 1:4 using the maceration method, and phytochemical screening was performed on the leaf extract. The antibacterial activity was tested against Staphylococcus aureus and Escherichia coli using the disc diffusion method. The results indicated that the matoa leaf extract contains various metabolites, including triterpenoids, steroids, flavonoids, phenolics, saponins, tannins, and alkaloids. Secondary metabolite compounds in matoa leaves are thought to be able to inhibit microbial growth so that inhibition zones can be formed. Furthermore, the antibacterial activity demonstrated that the matoa leaf extract effectively inhibited the growth of S. aureus and E. coli. the largest inhibition zone is of 18.22 mm at 10% extract concentration, and E. coli, with an inhibition zone largest is of 10.87 mm at 10% extract concentration. While the smallest inhibition zone in S. aureus is 15.29 mm at 7.5% concentration, and in E. coli is 5% at 5% extract concentration.. This study suggests that matoa leaves have the potential to serve as natural bioactive compounds and antimicrobial agents in the clinical industry. Keywords: Matoa, Phytochemical, Antibacterial activity, Staphylococcus aureus, Escherichia coli

IDENTIFIKASI TELUR CACING ENDOPARASIT PADA FESES AYAM KAMPUNG (Gallus gallus domesticus) RUMPUT GANAS MINI FARM, LOA JANAN ILIR, SAMARINDA Hasibuan, Febry Rahmadhani; Syahfitri, Widya; Br Hasibuan, Fitri Elizabeth Br Hasibuan; Ramadhani, Widiyanto; Ismail, Ahmad Yasin; Tsany, Alisha; Musdalifah, Yuni
Biosel Biology Science and Education Vol. 14 No. 2 (2025): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v14i2.9384

Parasites are divided into two based on their habitat, namely endoparasites and ectoparasites. Endoparasites are a group of parasites that live in the host's body to obtain food from the host. The spread of endoparasites in livestock can occur through contaminated feed, water, and livestock equipment. The purpose of this study was to identify the species of worm eggs in the feces of native chickens (Gallus gallus domesticus) at the Rumput Ganas Mini Farm, Loa Janan Ilir, Samarinda. 2g of chicken feces were taken and then 10 ml of NaCl solution was added. Homogenized and filtered using gauze. Poured into a centrifuge tube, then centrifuged for five minutes at a speed of 1500 rpm. The results of the centrifuge solution that settled on the surface were taken using a dropper pipette and then placed on an object glass. Covered using a cover glass, then observed under a microscope starting from the smallest magnification. The results of the study showed that two species of endoparasites were found in the feces of local chickens found in the mini farm loa janan ilir's vicious grass, namely Diphyllobothrium latum and Ascaridia galli. Keywords: Ascaridia galli, Dyphyllopbothrium latum, Endoparasite, Gallus gallus domesticus, Poultry Farming

Keanekaragaman Jenis Pohon dan Pola Penyebarannya pada Beberapa Wilayah Kawasan CA dan TWA Pananjung Pangandaran Azuhra, Fatimah; Fatina, Ikfina Nawal; Hasna, Chumairo Nurul; Hanifiyah, Nur; Azizy, Maulana Khalil; Muliyah, Evi
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.11189

The Pananjung Pangandaran Nature Reserve (CA) and Recreational Park (TWA) are in-situ conservation areas that play an important role in preserving biodiversity, particularly lowland forest flora along the southern coast of West Java. However, these areas are increasingly subjected to anthropogenic pressures resulting from tourism activities and population growth, which may affect ecosystem stability. This study aimed to analyze tree species diversity, identify tree distribution patterns, and assess abiotic components within the CA and TWA of Pananjung Pangandaran. The research employed a quantitative descriptive method with an exploratory approach using the line transect technique at three observation stations, namely coastal forest and lowland forest areas within the CA, as well as lowland forest areas within the TWA. A total of nine observation plots measuring 20 × 20 m were established. The results showed that tree species diversity in the western coastal forest of the CA, the lowland forest of Ciborok Block in the TWA, and the lowland forest of Cikamal Block in the CA was categorized as moderate, with Shannon-Wiener diversity index (H’) values ranging from 1.029 to 1.742 and a uniform distribution pattern. Overall, each observation station exhibited a distinctive vegetation composition, resulting from varying interactions between biotic and abiotic components. Keywords: Tree Species Diversity, Tree Distribution Pattern, Shannon-Wiener Index, Nature Reserve, Recreational Park

Pengaruh Penambahan Bayam Terhadap Uji Organoleptik Bakso Ikan Sako (Tylosurus sp) Kaliky, Nurainy; Mahelatu, Armina
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar

Sako fish (Tylosurus sp) is a type of pelagic fish with abundant availability in the waters of Maluku; however, its utilization as a processed food product is still limited. Considering its perishable nature, sako fish needs to be processed into products with a longer shelf life and appealing to consumers, one of which is fish meatballs. This study aims to determine the effect of adding spinach on the organoleptic characteristics of sako fish meatballs. The research method used is an experimental method with treatments involving the addition of spinach in sako fish meatballs and a control without spinach. Data analysis was performed using a paired sample t-test. The results showed that the organoleptic characteristics of sako fish meatballs had higher probability values for color, taste, and odor parameters compared to the original fish meatballs, but the texture was lower. This indicates that spinach can be used as a functional ingredient in making sako fish meatballs, thereby providing added value and product diversification. Keywords: Meatballs, Sako Fish, Spinach, Texture

Pengaruh Pemberian Variasi Media Pakan terhadap Produksi Berat Segar Maggot (Hermetia illucens) Faiza, Nurul; Ma'ruf, Muhammad Muizzul; Saiku Rokhim
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.11749

Hermetia illucens (Black Soldier Fly/BSF) is an insect capable of reducing organic waste through its larvae that consume various waste and produce biomass rich in protein and fat. Therefore, the quality of the feed media especially its protein content significantly influences the growth of these larvae. This study aims to identify the effect of variations in feed media on the fresh weight of BSF maggots to increase production efficiency as an alternative protein source. The study design used a Completely Randomized Design (CRD) with 27 experimental units replicated nine times. Six-day-old BSF larvae were reared for 14 days under controlled environmental conditions. Three types of feed media were used: tofu dregs, corn dregs, and vegetable waste. The results showed that the feed media significantly influenced the fresh weight of larvae, where the P3 (tofu dregs) treatment produced the highest weight of 23.5 grams, much higher than P1 (11.3 grams) and P2 (10.6 grams). The superiority of P3 is thought to originate from the nutritional content of tofu dregs, especially its higher crude protein, thus better supporting larval growth. Thus, selecting the right feed media is an important factor in increasing the efficiency of BSF maggot production as an alternative protein source. Keywords: Black Soldier Fly, Feed Media, Maggot Fresh Weight, Larvae Growth

A Adat, dan Harmoni: Studi Kasus Peran Kearifan Lokal “Lubuk Larangan” bagi Kelestarian Biodiversitas Ekosistem Sungai Masyarakat Mandailing Natal Rizki Khairani Nasution
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.11951

This study aims to examine the role of the local wisdom known as Lubuk Larangan in preserving the biodiversity of river ecosystems in Mandailing Natal, North Sumatra. Lubuk Larangan is a traditional practice that prohibits the catching of fish and other aquatic life in specific river areas for a period determined by the indigenous community. This tradition serves not only as a form of natural resource management based on local wisdom but also reflects the values of togetherness, sustainability, and respect for nature that have been passed down through generations. The research methodology uses a qualitative approach with data collection techniques that include in-depth interviews with customary leaders, community figures, and local fishermen, as well as participatory observation at the Lubuk Larangan sites. The data obtained are analyzed descriptively to understand how the system of values, norms, and customary sanctions is applied in this conservation mechanism. The results of the study show that the Lubuk Larangan tradition is proven effective in maintaining the sustainability of river ecosystems. This tradition not only limits the exploitation of aquatic resources through clear customary rules but also serves as an environmental education medium for the younger generation, strengthens social bonds within the community, and maintains ecological balance. The active participation of the community, adherence to customary norms, and the enforcement of strict social sanctions are the main factors supporting the success of this practice. Thus, this research confirms that Lubuk Larangan is not just a local cultural heritage but also a traditional conservation mechanism that is relevant to the concept of sustainable development. The integration of this local wisdom with modern natural resource management policies can be an effective model for preserving biodiversity and the harmony between humans and nature. Keywords: Tradition, Lubuk Larangan, Local Wisdom, Conservation, Biodiversity, Mandailing Natal

DNA Barcoding sebagai Inovasi Bioteknologi untuk Identifikasi dan Konservasi Tanaman Lokal: Suatu Tinjauan Sistematis Syahriani; Hala, Yusminah
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.12116

Limitations in accurate and efficient species identification techniques at various stages of development and sample conditions are major problems in the conservation of local plants. Conventional morphology-based identification approaches often encounter obstacles due to phenotypic variation and diagnostic character limitations. This study aims to examine the potential of DNA barcoding as a biotechnology innovation in supporting the identification and conservation of local plants. The research method used was a systematic literature study of national and international scientific articles published between 2020 and 2025. Data sources were obtained from the PubMed, Scopus, ScienceDirect, and Google Scholar databases, then analyzed thematically by grouping findings based on the type of genetic marker, identification procedure, and relevance to plant conservation. The results of the study show that DNA barcoding is a species identification technique carried out through the analysis of deoxyribonucleic acid sequences from specific standard marker genes. In plant taxonomy, chloroplast genes commonly used as genetic markers include ribulose bisphosphate carboxylase large chain, maturase K, and internal transcribed spacer. The genetic sequences obtained are then compared with a global database to accurately determine species suitability. This technique has been proven to provide fast and reliable species identification under various sample conditions. With these advantages, DNA barcoding has great potential as a key supporting tool in local plant conservation efforts. Keywords: DNA Barcode, Biotechnology, Plant Conservation

Variasi Lingkungan Perairan dan Respons Komunitas Lamun di Pulau Pahawang Lestari, Tridyah; Aryawati, Riris; Aryawati, Wike Ayu Eka; Melki; Purwiyanto, Anna Ida Sunaryo
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.12453

Seagrass ecosystems play a crucial role in maintaining coastal environmental stability, yet their presence in Pahawang Island shows a declining trend due to human activity pressures and changes in aquatic conditions. This study aims to describe the structure of the seagrass community and analyze its correlation with environmental parameters at four stations with different characteristics. Seagrass data collection was conducted using the transect-quadrant method to measure coverage, density, and species composition, while environmental parameters observed included temperature, salinity, pH, dissolved oxygen (DO), current, and water clarity. The results showed the presence of three seagrass species, namely Enhalus acoroides, Thalassia hemprichii, and Halodule uninervis, with varying coverage and density across locations; the best condition was found at the natural station and the lowest at the pier area. The diversity index was classified as low, while uniformity and dominance varied according to human activity levels around the water. PCA analysis indicated that salinity, current, clarity, DO, pH, and temperature contribute differently at each station and collectively influence the variation in the seagrass community. These findings emphasize that differences in environmental quality and the intensity of anthropogenic disturbances are key factors determining the condition of the seagrass beds in Pahawang Island. Keywords: Seagrass, Water Quality, Community Structure, PCA, Pahawang Island

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