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Biosel: Biologi Science and Education

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Published by Institut Agama Islam Negeri Ambon

ISSN : 2252858X EISSN : 25411225 DOI : -

Core Subject : Science, Education, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science

Journal Biosel receiving articles of the results of the study in biology field and education. This journal rising twice a year, namely edition of january-june and edition of july-december.

Arjuna Subject : -

Articles 304 Documents

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Analisis Struktur Komunitas Fitoplankton di Beberapa Ekosistem Air Terjun Kecamatan Jabung, Kabupaten Malang Salsabila, Dina; Catur Retnaningdyah; Yusuf, Muhammad
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.12791

Phytoplankton are primary biotic components of aquatic ecosystems that are highly sensitive to environmental changes and are therefore widely used as biological indicators of water quality. However, information on phytoplankton community structure in waterfall ecosystems remains limited, particularly in tropical regions. This study aimed to analyze the phytoplankton community structure in waterfall ecosystems in Jabung district as a basis for assessing water quality. Sampling was conducted at four waterfall sites, namely Siuk, Tarzan, Toris, and Jahe, with three replicates at each location. Phytoplankton samples were collected by filtering 3 liters of water using a plankton net with a mesh size of 40 µm, then preserved with 4% formalin (10 drops) and CuSO₄ (5 drops). Identification and enumeration of phytoplankton were performed using 1 mL of sample placed in a Sedgewick–Rafter Counting Chamber and observed under a binocular microscope (100× magnification) across 1,000 fields of view. The collected data were used to calculate abundance, taxonomic richness, community composition, the Shannon–Wiener diversity index (H’), evenness index (E), and dominance index (D). The results showed that phytoplankton taxa were present in all observed waterfall ecosystems, consisting of Bacillariophyceae (21 genera), Chlorophyceae (9 genera), Cyanophyceae (5 genera), Zygnematophyceae (9 genera), and Trebouxiophyceae (1 genus). The highest diversity index was recorded at Jahe Waterfall (H’ = 4.50), while the lowest was found at Siuk Waterfall (H’ = 4.17). The highest evenness index occurred at Siuk Waterfall (E = 0.8773), with a dominance index value of 0.075. Overall, all sites exhibited high diversity (H’ > 3) and very low dominance, indicating that phytoplankton communities were stable and well balanced across all investigated waterfall ecosystems. Keywords: Water Quality, Phytoplankton, Community, Waterfalls, Diversity

Species Diversity of Lichen as A Bioindicators of Air Quality at The State Polytechnic of Jember Agustin, Mili; Avika Aditya Febriana Putri; Ika Nuraini; Yohana Maharani Pitaloka; Putri Saniyyah Rahmalisa Az Zahra; Retno Wimbaningrum; Rendy Setiawan
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.12855

Lichens are symbiotic organisms that are sensitive to changes in air quality, so they are widely used as environmental bioindicators. The purpose of this study was to determine the diversity of lichens in the Jember State Polytechnic area and to assess air quality based on the Atmospheric Purity Index (API). The research was conducted at nine observation sites using a purposive sampling method with 10 × 10 cm plots on four sides of tree trunks at each point. Lichen samples were collected, identified based on morphological characteristics, and their coverage percentage was calculated to obtain frequency values and ecological indices used in the ACI calculation. The results showed that 25 types of lichens were found with a coverage area of 8.647%. The highest ACI value was found in the Department of Language, Communication, and Tourism with a value of 0.35, while the lowest values were found in the Department of Information Technology and the Department of Agribusiness Management with ACI values of 0.02, respectively. All locations on campus had very low IAP values with a total value of 0.86, indicating a very high level of air pollution. The location with the highest IAP value remained in the very high pollution category. The low IAP value is strongly suspected to be related to the high level of motor vehicle activity on campus and the suboptimal function of vegetation in reducing atmospheric pollutants. Keywords: Air Quality, Atmospheric Purity Index, Bioindicator, Lichen

The Influence of the Problem Based Learning (PBL) Model on Metacognitive and Learning Outcomes of Class VIII Students of SMP Negeri 37 Samarinda on Human Excretory System Material Jusma; Nasution, Ruqoyyah; Akhmad; Masitah
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.7491

Facing future challenges requires various skills to achieve success, one of which is metacognitive skills. Therefore, the use of science-based learning in the learning process not only aims to improve student learning outcomes, but also to empower their metacognitive skills. This research aims to determine the effect of using Problem Based Learning (PBL) on the metacognitive and learning outcomes of class VIII students at SMP Negeri 37 Samarinda. This type of experimental research uses quantitative methods in the form of a quasi experimental design type, the pretest posttest control group design. The population in this study were all class VIII students at SMP Negeri 37 Samarinda. Sampling used a purposive sampling technique using classes according to the researcher's needs with a sample size of 48 students. The instrument analysis technique uses construct validity and reliability tests, while the analysis of research results uses the N-gain test, normality test, homogeneity test, and Wilcoxon test with a significance level of 5%. The results of data analysis show that the average value of students' metacognitive indicators after being treated in the experimental class was 80.67 in the very good category, while the control class was 79.58 in the good category. The average posttest score for experimental class learning outcomes was 69.82, while the control class was 38.02. The N-gain metacognitive questionnaire test in the experimental class was 0.27 in the low category, while the control class was 0.08 in the low category. The experimental class had an N-gain value for the cognitive learning outcomes test of 0.46 in the medium category, while the control class was -0.01 in the low category. The Wilcoxon test results on the metacognitive questionnaire and learning outcomes show the Asymp value. Sig. (2 tailed) of 0.000, this value is smaller than 0.05 so it can be concluded that there is an influence of the use of Problem Based Learning (PBL) on metacognitive and cognitive learning outcomes of class VIII students at SMP Negeri 37 Samarinda on the human excretion system material. Keywords: Problem Based Learning, Metacognitive, Learning Outcomes

Mapping the Nexus of Etnobotany and Education: A Bibliometric Approach to Advancing 21st-Century Skills through Etnopedagogy Yani, Ahmad; Makkatenni; Rijal, Syamsu
Biosel Biology Science and Education Vol. 15 No. 1 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i1.11564

The integration of ethnobotany and education through ethnopedagogy is increasingly acknowledged as a promising strategy to foster the development of 21st-century skills, such as critical thinking, creativity, collaboration, and cultural literacy. Nevertheless, a comprehensive mapping of the research landscape connecting these fields remains scarce. This study seeks to analyze global research trends, intellectual structures, and thematic developments in studies that link ethnobotany, education, and ethnopedagogy. A bibliometric analysis was conducted using peer-reviewed articles indexed in Scopus and Web of Science that were published between 2010 and 2025. Data were analyzed using Bibliometrix R package and VOSviewer to examine publication growth, influential authors and journals, institutional contributions, and keyword co-occurrence networks. The findings indicate a consistent increase in the number of publications over the past decade, with major research clusters focusing on indigenous knowledge systems, plant-based science education, and community-oriented pedagogical practices. Thematic evolution analysis revealed a shift from descriptive documentation of ethnobotanical knowledge to integrative educational frameworks that embed local plant knowledge within formal and informal learning contexts. Collaboration networks demonstrate increasing transnational partnerships, particularly among scholars from biodiversity-rich regions in Asia, Africa, and Latin America. Despite this progress, empirical studies evaluating the impact of ethnobotanical learning on students’ 21st-century skills remain limited. This study provides a comprehensive knowledge map and highlights future research directions to strengthen the integration of ethnopedagogy in science education. Keywords: Ethnobotany, Ethnopedagogy, Biology Education, Bibliometric Analysis, 21st-Century Skills

Synergistic Anti-Biofilm Activity of Stingless Bee Honey and Binahong Leaves Extract Against Staphylococcus aureus Fieona; Tiara Halidah Ratnasari; Paula Mariana Kustiawan
Biosel Biology Science and Education Vol. 15 No. 2 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i2.13805

Biofilm formation by Staphylococcus aureus is a major virulence factor contributing to antibiotic resistance. This study investigated the synergistic anti-biofilm activity of stingless bee honey (Homotrigona apicalis) combined with binahong leaves extract (Anredera cordifolia) against S. aureus in vitro. Binahong leaves were macerated using 96% ethanol. Anti-biofilm activity was assessed using a microbroth dilution crystal violet assay in 96-well plates. Five combination ratios (100% Binahong [K1], 100% honey [K2], 75:25 [K3], 50:50 [K4], and 25:75 [K5]) were tested at six concentrations (0.25–8%) and incubated at 37.5°C for 24, 48, and 72 hours. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, tannins, and terpenoids. All combinations exhibited concentration-dependent anti-biofilm activity, with the 50:50 combination (K4) showed the most stable and effective inhibition profile across all concentrations and incubation times. The minimum biofilm inhibitory concentrations for 50% and 90% inhibition (MBIC₅₀ and MBIC₉₀) were 0.25% and 8%, respectively. These results demonstrate that stingless bee honey (Homotrigona apicalis) and Binahong leaves extract exhibit strong synergistic anti-biofilm activity, highlighting their potential as alternative agents against S. aureus biofilms. Keywords: Anti-biofilm, Synergistic, Honey, Binahong, Staphylococcus aureus

Microbiological Assessment of Street Foods: Detection of Staphylococcus aureus and Salmonella spp Using Selective Culture Methods Liss Dyah Dewi Arini; M. Ekahidayatullah; Annisa Rahmawati; Anggraini Sih Prabandari; Mahjanah Husein
Biosel Biology Science and Education Vol. 15 No. 2 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i2.13806

Food safety remains a major public health concern due to the risk of contamination by pathogenic microorganisms. Street food, which is widely consumed, has a high potential for microbiological contamination due to inadequate hygiene and sanitation practices. This study aimed to detect and estimate the presence of presumptive Staphylococcus aureus and presumptive Salmonella spp. in street food samples. A quantitative descriptive laboratory approach was employed using the spread plate method on selective media, namely Mannitol Salt Agar (MSA) and Sorbitol MacConkey Agar (SMAC). Ten types of street food samples were analyzed using serial dilutions (10⁻¹, 10⁻², and 10⁻³) with duplicate treatments. The results showed that most samples exhibited high levels of microbial contamination, with Total Plate Count (TPC) values ranging from 0 to 2.16 × 10⁶ CFU/g. Several samples exceeded the maximum microbial contamination limit of 10⁴ CFU/g established by the Food and Drug Supervisory Agency (BPOM). The presence of colonies morphologically identified as presumptive Staphylococcus aureus and presumptive Salmonella spp. indicates potential contamination by pathogenic bacteria. These findings suggest that street food may pose a risk to public health if hygiene and sanitation practices are not properly maintained. Therefore, improved food handling practices and monitoring are necessary to ensure food safety. Keywords: Food Safety, Street Food, Staphylococcus aureus, Salmonella spp., Total Plate Count, Microbial Contamination

Carbon Stock Mapping in the Area of West Oesapa Mangrove Forest Ecotourism using Sentinel-2a Imaginery Maria Edelin; Yofris Puay; Aah Ahmad Almulqu; Dimaz Danang Al-Reza; Khozanah Syifa; Dwi Wahyuni; Anasufi Banawi
Biosel Biology Science and Education Vol. 15 No. 2 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i2.13822

This research is motivated by the prevailing issue of global warming, characterized by the increase in the average temperature of the Earth's atmosphere, leading to climate change. In climate change mitigation efforts, mangrove forests play an important role by effectively storing and absorbing substantial quantities of carbon, subsequently sequestering it in the form of biomass. This study aims to estimate the potential of carbon stocks stored in the area of West Oesapa Mangrove Forest Ecotourism, estimate CO2 uptake, and determine the correlation between NDVI vegetation index and carbon stock/storage. This research was conducted for two months, started from November to December 2023 at the West Oesapa Mangrove Forest Ecotourism. The research stages consisted of pre-image processing, image processing, field survey, and data analysis. The species allometric formula was utilized to calculate mangrove biomass, which was then converted into carbon stock values in mangrove forests. Sentinel-2A images were transformed with Normalized Difference Vegetation Index (NDVI), then regression analysis was conducted between NDVI and carbon stock values to obtain a carbon stock estimation model using satellite imagery. There are five mangrove species found, namely Sonneratia alba, Avicenia alba, Rhizophora stylosa, Rhizhopora mucronate, and Burguiera. The results indicated that the estimated carbon stock stored in the West Oesapa Mangrove Forest Ecotourism was 3,852.36 tons/ha. Meanwhile, the estimated CO2 uptake in the West Oesapa Mangrove Forest Ecotourism is 14,125.32 tons/ha. The results of the person correlation analysis with a correlation coefficient of 0.608 means that the NDVI variable and carbon stocks are correlated or have a relationship. Keywords: Biomass, Mangrove Carbon, Allometric Calculation, Sentinel 2A Imagery, NDVI

Development and Validation of a Principal Performance Assessment Instrument to Support Technology-Enhanced Biology Learning and Environmental Literacy in Secondary Schools Ivatul Laily Kurniawati; Dhamas Mega Amarlita; Mursaid Dahlan
Biosel Biology Science and Education Vol. 15 No. 2 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i2.13823

This study aims to develop and validate a performance assessment instrument for school principals to evaluate their effectiveness in supporting technology-enhanced biology learning and environmental literacy at the secondary school level. The research employed a Research and Development (R&D) approach consisting of four major phases: needs analysis, construct formulation and indicator development, expert validation, and field testing. The instrument was constructed based on nine domains of instructional leadership relevant to 21st-century biology education: learning outcomes, curriculum management, content organization, learning processes, independent learning, technological support, supervised learning visits, adaptability in instructional implementation, and learning evaluation. Expert judgment indicated strong content validity across all domains, with minor revisions suggested for clarity. Field testing conducted in several secondary schools demonstrated that the instrument possesses high internal consistency, indicating reliable measurement. The findings reveal that the instrument is capable of capturing critical dimensions of leadership performance that influence the quality of biology learning, particularly in relation to technology integration and environmental literacy. The developed instrument is recommended for use as an evaluative tool and as a basis for designing professional development programs to enhance school principals’ capacity to lead adaptive, contextual, and sustainability-oriented biology education. Keywords: Principal performance, Instructional leadership, Biology education, Environmental literacy, Educational technology

Spatial Variation and Community Structure of Trichoptera Larvae as a Basis for Biomonitoring in the Bone River, Gorontalo Miftahul Khair Kadim; Nuralim Pasisingi; Abdul Hafidz Olii
Biosel Biology Science and Education Vol. 15 No. 2 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i2.13838

This study aimed to examine the community structure of Trichoptera larvae in the Bone River, Gorontalo (Indonesia), by evaluating family-level composition, dominance patterns, and spatial variation across five sampling stations (St.1-St.5). Trichoptera larvae were collected using standardized sampling methods, identified to the family level, and analyzed using multiple diversity metrics, including the Shannon, Simpson, Evenness, and Margalef indices. Differences in taxon richness among stations were assessed using rarefaction analysis, while community structure patterns were further explored using multivariate approaches based on Bray–Curtis dissimilarity, non-metric multidimensional scaling (NMDS), and hierarchical cluster analysis. A total of 754 individuals representing eight families were identified. Hydropsychidae strongly dominated the community, accounting for more than 70% of the total individuals. Diversity indices revealed spatial variation in community balance, with Station 5 exhibiting the highest diversity and evenness values, indicating a relatively more balanced community structure. Rarefaction analysis suggested that differences in richness were not solely driven by sampling effort. Multivariate analyses based on Bray-Curtis dissimilarity, hierarchical clustering, and NMDS demonstrated spatial differentiation in community composition along the river gradient, largely associated with variations in family dominance. Water quality parameters (temperature, pH, and dissolved oxygen) were within national quality standards, indicating conditions generally supportive of aquatic life. The observed variation in community structure therefore likely reflects local habitat dynamics rather than broad physicochemical stress. This study provides baseline data on Trichoptera communities in the Bone River and highlights the ecological importance of Hydropsychidae in shaping community structure, supporting the potential application of Trichoptera as biomonitoring indicators in riverine ecosystems. Keywords: Trichoptera, River, Community Structure, Spatial Variation, Biomonitoring

A Scoping Review of Generative AI Reenactment in Biology Education: Approaches, Practices, and Challenges Maulana Arsyadani Bintang Pratama Putra; Slamet Hariyadi
Biosel Biology Science and Education Vol. 15 No. 2 (2026): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v15i2.13841

The integration of Generative AI (GenAI) Reenactment in biology education offers potential for interactive simulations, yet details regarding its practical use and effectiveness outcomes have not been comprehensively documented. This study aims to map the technological approaches, pedagogical practices, and challenges of using GenAI Reenactment in biology learning through a scoping review method. The method utilizes the Arksey & O’Malley and PRISMA-ScR frameworks, enriched with the Reflexive Thematic Analysis approach by Braun and Clarke. A literature search (2023–2025) across reputable databases yielded 4 primary articles meeting the inclusion criteria. Data analysis identified three key themes: (1) Evolution of Technological Approaches, ranging from text-based Large Language Models for role-playing interactions to indications of integration with Virtual Reality (VR) and Augmented Reality (AR); (2) Transformation of Pedagogical Practices, where GenAI simulates roles as a self-directed learning tool and potentially as a Virtual Teaching Assistant (VTA) in laboratories; and (3) Multidimensional Challenges, highlighting risks of declining psychomotor skills and student communication originality due to reliance on instant answers. It can be concluded that GenAI Reenactment demonstrates potential as an interactive evolution of previous visualization technologies, necessitating a hybrid supervision model. Keywords: Generative AI Reenactment, Biology Education, Virtual Teaching Assistant, Reflexive Thematic Analysis.

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