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Klinikal Sains (Jurnal Analis Kesehatan)
Published by Universitas Abdurrab
ISSN : 23384921     EISSN : 26141515     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology
Jurnal klinikal sains program studi analis kesehatan adalah jurnal ilmiah yang membahas tentang kompetensi analis kesehatan yang meliputi kompetensi Mikrobiologi, Hematologi, Parasitologi, Imunoserologi, Toxikologi, dan Kimia Klinik. Jurnal klinikal sains akan terbit setiap enam bulan, dalam satu periode setiap tahun ajaran akan terbit dua kali yakni pada bulan Juni dan Desember.
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Articles 20 Documents
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Search results for , issue "vol. 12 no. 2 (2024): desember" : 20 Documents clear
HUBUNGAN PERILAKU HIDUP BERSIH DAN SEHAT (PHBS) DENGAN INFEKSI SOIL TRANSMITTED HELMINTHS (STHs) PADA ANAK SD DI TIWOHO KECAMATAN WORI Indra Elisabet Lalangpuling; Mouren Irene Yusuf; Desinta Samala Majampoh; Kevin Giovani Pascoal; Michael V.L. Tumbol; Suwarja Suwarja; Rosdiana Naibey
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4916

Abstract

One of the factors that causes Soil Transmitted Helminths infection (intestinal nematodes that are transmitted through soil) is Clean and Healthy Living Behavior (PHBS). STHs usually breed in tropical areas with high temperatures and humidity. The aim of this research was to find out whether STHs worm infection which was transmitted through soil in elementary school children in Tiwoho Village, Wori District was related to clean and healthy living behavior (PHBS). This type of research is descriptive with a sampling method, namely purposive sampling of 65 stool samples that meet the inclusion criteria. Stool samples were examined microscopically using the native method, flotation method and Baerman Test method. The Square test was used in data analysis to assess whether there was a relationship between PHBS and STHs worm infection. The results of this study show that there is a relationship between Clean and Healthy Living Behavior (PHBS) and STHs worm infection, especially the habit of washing hands with soap before eating and the habit of biting nails with a p value <0.005.
UJI AKTIVITAS ANTIOKSIDAN EKSTRAK AIR TANAMAN SEMANGGI (Marsilea crenata C.Presl) METODE FRAP (Ferric Reducing Antioxidant Power) raisya adila adila; I.A.K. Pramushinta; A.S. Sinulingga
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4952

Abstract

The clover plant (Marsilea crenata C.Presl) is a plant that is widely used for food and has the potential to be developed as a herbal medicine. Clover plant extract using methanol, chloroform, and ethyl acetate was found to have weak antioxidant activity. The aim of this research was to test the antioxidant activity in air solvent extracts of clover plants using the FRAP (Ferric Reducing Antioxidant Power) method and analyze the effect of variations in extract concentration on the antioxidant activity test. The method in this research is clover plant extraction by maceration using an air solvent. Test antioxidant activity using the FRAP method using a UV-Vis spectrophotometer with a maximum wavelength of 596 nm. The extract yield of 42% showed that it positively contained flavonoids, saponins, polyphenols and a water content of 9.94% ± 0.1276. The results showed that clover plants had weak antioxidant activity (IC50 351.04 ppm) compared to vitamin C as a positive control which showed very strong antioxidant activity (IC50 22.60 ppm). From the results of this study, it can be said that increasing the extract concentration can increase the percentage of inhibition of free radical formation, but the increase is not linear, as indicated by the R2 value not approaching 0.99.
PERBANDINGAN PERTUMBUHAN Aspergillus niger PADA MEDIA POTATO DEXTROSE AGAR (PDA) DAN MEDIA ALTERNATIF DARI SINGKONG, UBI JALAR, DAN BEKATUL Indah Lestari; Akhmad Mubarok; Imam Agus Faizal
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4955

Abstract

Aspergillus niger is one of the fungi that can produce citric acid and is often used in some studies because of its resistance to very low acidic conditions. Potato Dextrose Agar medium is a common medium for culturing fungi in the laboratory. Alternative media are media that use materials from nature whose content is not yet known in detail. Rubber cassava has a carbohydrate content of 98.4674%. The carbohydrate content of white sweet potato is 28.78%. Rice bran has a carbohydrate content of 84.36%. The purpose of this study was to determine the comparison of the growth diameter of Aspergillus niger fungi on Potato Dextrose Agar (PDA) media and alternative media made from cassava, sweet potato, and rice bran. The research used a pure experimental method. Aspergillus niger isolates were observed microscopically, after which they were planted on PDA media and alternative media, incubated at 37°C for 72 hours, then the diameter of fungal growth was measured. The results showed that the alternative media that had the largest Aspergillus niger fungal growth diameter compared to PDA media as a control was alternative media from rice bran with the smallest diameter of 40 mm and the largest diameter of 80 mm.
PERBANDINGAN KADAR HEMOGLOBIN, GLUKOSA DARAH, DAN LEUKOSIT SEBELUM DAN SESUDAH HEMODIALISA PADA PASIEN GAGAL GINJAL KRONIS KOMORBID DAN NON KOMORBID DIABETES MELLITUS DI RSPC Risma Adzkia Putri; Ira Pangesti; Meka Faizal Farabi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4956

Abstract

Chronic Kidney Disease (CKD) is an impairment of the glomerular organ function of the kidneys. The process of blood purification by collecting waste is called hemodialysis. The prevalence of CKD increased from 1990 to 2017 globally by 21.3% and the mortality rate increased by 41.5%. The purpose of this study was to compare hemoglobin, leukocyte, and blood glucose levels before and after hemodialysis in patients with and without comorbid Diabetes Mellitus (DM) at Pertamina Cilacap Hospital (RSPC). The method used was cross sectional using univariate and bivariate analysis with JASP program. The results showed that in CKD patients without DM, hemoglobin levels were significantly different (p<0.05) after hemodialysis. While leukocyte and glucose levels had no significant difference (p>0.05). In CKD patients with DM, there is a significant difference in hemoglobin levels (p<0.05) after hemodialysis. In addition, there is a significant difference in glucose levels (p<0.05) after hemodialysis. Meanwhile, there was no significant difference in leukocyte levels (p>0.05). Comparison of pre and post hemodialysis between CKD without DM and CKD with DM there is a significant difference (p<0.05) in hemoglobin and blood glucose levels but there is no significant difference (p>0.05) in leukocyte levels.
UJI AKTIVITAS ANTIOKSIDAN INFUSA SEMANGGI (Marsilea crenata C. Presl) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-PicrylHydrazyl) Tiara Juliar Subakti; IAK Pramushinta; AS Sinulingga; SA Devi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4966

Abstract

Changes in lifestyle and environment now increase the risk of exposure to free radicals that cause degenerative diseases. Compounds needed to neutralize free radicals are antioxidants. One source of antioxidants is clover (Marsilea crenata C. Presl), which has previously been studied and has shown no antioxidant activity in various extraction solvents, but the clover infusion method with water solvent has not been studied, so the purpose of this study was to determine the antioxidant activity of clover infusion (Marsilea crenata C. Presl). This research method uses the DPPH method, then the antioxidant activity test of clover infusion (10% b/v) with a concentration of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, and vitamin C as a positive control measured by a UV-Vis spectrophotometer. The absorbance value obtained at the maximum DPPH wavelength (516 nm) was used to calculate the linear regression equation of concentration and% inhibition. The results of the study obtained the IC50 value of clover infusion of 804.0 ppm and vitamin C as a positive control of 29.1 ppm. The conclusion of the study is that clover infusion shows no antioxidant activity (IC50 > 500 ppm), while vitamin C shows very strong antioxidant activity (IC50 > 50 ppm). Further research suggestions are related to the development of clover plant extraction methods and solvents (Marsilea crenata C. Presl) and antioxidant activity test methods.
ANALISIS PERBANDINGAN KADAR FLAVONOID TOTAL TEH HIJAU DAN TEH HITAM DENGAN MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS Antonia Vani Kurniawati; .A.K Pramushinta; A.S. Sinulingga; Novamei Indriani
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4986

Abstract

Tea leaves (Camellia sinensis) are a functional plant that is useful for preventing cancer, maintaining immunity and preventing premature aging. Flavonoids are one of the largest groups of polyphenols which are active compounds from tea leaves (Camellia sinensis). This study aims to determine the total flavonoid content of green tea leaf extract and black tea leaf extract using 2 extraction methods, namely maceration with 96% ethanol solvent and infusion using water solvent. The method in this research uses qualitative tests (Wilstarter cyanidin and NaOH 10%) and quantitative tests (UV-Vis Spectrophometry) and conclusions will be drawn using the SPSS statistical test T (independent)-test. The results of the qualitative test using the starter cyanidin and 10% NaOH were positive for containing flavonoids due to a reddish color change. In the quantitative test, the assay was carried out using UV-Vis spectrophotometry with a wavelength of 434 nm. In this study, the total flavonoid content obtained in the green tea maceration method was 15.728% w/w ± 0.209, black tea maceration 13.357% w/w ± 0.737, green tea infusion 4.153% w/w ± 0.124 and black tea infusion 3.320% w /b ± 0.352. There is a significant difference between green tea and black tea using both the infusion and maceration methods as shown by a sig value ≤ 0.05.
PENGARUH JENIS PELARUT EKSTRAKSI TERHADAP KADAR ALKALOID DALAM EKSTRAK TERIPANG (Paracaudina australis) MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS Aulya Sagita Rahma Sujana; Prisma Trida Hardani; Amanda Safithri Sinulingga
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.4993

Abstract

Sea cucumbers are a type of marine organism that move slowly and live in shallow waters such as sand, mud, algae, coral reefs where sea cucumbers are a source of animal protein consumed by the public. Research on various types of sea cucumbers has shown that there are several bioactive compounds, one of which is alkaloids. Alkaloids are one of the secondary metabolite compounds that are often found in nature, such as in plant and animal tissues. Previous research results show that there are alkaloids in sea cucumber extract. The aim of this research is to determine how this limited type of solvent impacts the amount of bioactive compounds contained in sea cucumbers (Paracaudina australis). Qualitative and quantitative tests were carried out to test alkaloid compounds. Qualitative tests use color reactions with Dragendroff, Wagner and Mayer reagents. Quantitative tests were carried out using Uv-Vis spectrophotometry with a maximum wavelength of 273 nm. The standard solution used is caffeine. Quantitative analysis showed that all extracts with various solvents were positive for containing alkaloids. The highest alkaloid content is ethyl acetate extract with levels of 71.0116 ± 0.2872 ppm, then followed by acetone extract which is semipolar with levels of 22.6531 ± 0.2277 ppm and the lowest is chloroform extract with levels of 13.9622 ± 0.0283 ppm. Keywords: Sea cucumber; Alkaloids; Solvent Type; Uv-Vis Spectophotometry
ANALISA PEMANTAPAN MUTU INTERNAL TAHAP PRA-ANALITIK PEMERIKSAAN HEMATOLOGI Bastian Bastian; Aziyo Kurniawan; Aristoteles Aristoteles
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5013

Abstract

Stages pre-analytic are stages beginning in an inspection laboratory. Errors on stage pre-analytic account for 70% of frequent errors in the laboratory. To anticipate errors, the so-done effort stabilizes quality. To increase quality service and repair quality results. The Objectives: Is to stabilize internal quality during the pre-analytic stage of hematology in the laboratory at House Sick Type C, Palembang. Study this method is descriptive-quantitatvie with a purposive sampling technique in the officer laboratories “X” and “Y”, respectively total 15 respondents. Results: In the “X” hospital laboratory has an average result of 96% and is said to be good, while in the “Y” hospital laboratory, it has an average 97% and is said to be good. That there is no significant difference between the second House Sick. Conclusion: Stabilization of internal quality stage pre-analytical inspection hematology on officers in the laboratories of Hospital “X” and Hospital “Y” Palembang can be stated as good.
PROFIL LIPID PADA LANJUT USIA SEBAGAI FAKTOR RESIKO PENYAKIT METABOLIK DI KENDARI SULAWESI TENGGARA Ani Umar; Firdayanti Firdayanti; Angriani Fusvita
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5089

Abstract

Metabolic diseases are a global health problem that is increasing especially in developed countries (Silva et al., 2020). The incidence is higher in the elderly population due to physiological and metabolic changes associated with aging (Silva et al., 2020). Lipid profile is an important indicator in assessing the risk of metabolic diseases. Blood lipids include total cholesterol, LDL (Low Density Lipoprotein), HDL (high Density Lipoprotein), and triglycerides, playing a major role in the development of cardiovascular disease. (Emmanuel et al., 2022) The purpose of this study was to determine the lipid profile in the elderly as one of the metabolic diseases. This study used a cross-sectional design with a target population of 50 elderly individuals aged 60 years and over. data were collected through direct measurement of lipid profiles including total cholesterol, triglycerides, HDL, and LDL using the CHOD-PAP method (Chlorimetric Derivate- Para Amino Phenazone) using a spectrophotometer. The results of the study showed that from 50 elderly patients, the lipid profile levels obtained were total cholesterol levels in the normal category with an average cost of 1.66, where 66% of respondents obtained normal cholesterol levels, while 34% showed high cholesterol levels. in the triglyceride variable, the results obtained were 72% of respondents had normal triglyceride levels, while 28% experienced an increase. HDL levels showed more positive results, with 82% of respondents having good HDL levels, and only 18% having low HDL levels, and, analysis of LDL levels revealed that only 8% of respondents had high LDL levels, while 92% had normal LDL levels in men and women, TG/HDL limits are <150 and> 40 mg/dl, and cholesterol limits <200 and LDL <150.
PERBANDINGAN HASIL PEMERIKSAAN KOLESTEROL TOTAL MENGGUNAKAN SAMPEL SERUM DAN PLASMA EDTA SEGERA DAN DISIMPAN SELAMA 4 JAM Luvia Isma Hanifah; Dini Puspodewi; Ira Pangesti
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5111

Abstract

Cholesterol is a lipid substance (fat component) being one among other known components, fat being a nutrient that is fulfilled by the body. The purpose of the study was to determine the comparative results of total cholesterol examination using serum and plasma EDTA immediately and stored for 4 hours. This study is analytic using cross sectional research design. The results showed in statistical tests using the Paired Sample T-Test test on serum samples immediately examined and stored for 4 hours found (p=0.015) so that the results (p>0.05) concluded there was no difference in serum samples. EDTA plasma samples immediately examined and stored for 4 hours found the results (p<0.001), comparison of total cholesterol levels using serum and EDTA plasma samples immediately examined and stored for 4 hours found (p<0.001) so that the results (p<0.05) are concluded there is a difference between total cholesterol levels in EDTA plasma immediately examined and stored for 4 hours and serum and EDTA plasma immediately examined and stored for 4 hours. Key Words : Cholesterol, Serum, EDTA Plasma, 4 Hour Delay

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