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Menara Perkebunan

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Published by Pusat Penelitian Kelapa Sawit

ISSN : 01259318 EISSN : 18583768 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Menara Perkebunan as a communication medium for research in estate crops published articles covering original research result on the pre- and post-harvest biotechnology of estate crops. The contents of the articles should be directed for solving the problems of production and/or processing of estate crops of smallholder, private plantations and state-owned estates, based on the three dedications of plantation. Analyses of innovative research methods and techniques in biotechnology, which are important for advancing agricultural research. Critical scientific reviews of research result in agricultural and estate biotechnology.

Arjuna Subject : -

Articles 541 Documents

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Identifikasi homolog TcAGL-15 untuk penanda embriogenesis tanaman kakao melalui pendekatan bioinformatika Identification of TcAGL-15 homolog in the embryogenic culture from the zygotic embryo explant Oktaviany Ferry TRIASTANTO; Muhammad JUSUF; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 74, No 2: Desember 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary One of the major problems encountered in micropropagation of cacao through tissue culture is very low frequency of embryo formation. Embryogenesis is believed to have key regulatory gene determining the process. Understanding such gene may help to solve problems in the regeneration process. One of the genes reported to involve in the embryogenesis is AGAMOUS-like 15 (AGL-15). This gene has an important role in the regulation of early embryogenesis in several plants. This experiment aimed to identify AGL-15 homolog in cacao through bioinformatics approach. The first step of this experiment is to identify the AGL-15 homolog using hetero-logous primers from DNA genomic isolated from leaves of cacao plants. The sequence of the AGL-15 fragment was used in designing specific primers for longer AGL-15 fragment. These primers were then used to identify AGL-15 gene using total RNA isolated from cultured zygotic embryos. Differential pattern of AGL-15 gene expression was observed in zygotic embryos cultured for five weeks. AGL-15 heterologous primers designed from several plants could be used to identify cacao AGL-15 homolog. The putative cacao AGL-15 gene could be identified from zygotic embryos. The differential pattern of the AGL-15 gene expression is a strong indication that AGL-15 can be used as an embryogenesis marker in cacao plants.Ringkasan Salah satu kendala perbanyakan kakao melalui kultur jaringan adalah sulitnya embriogenesis, yang diduga melibatkan satu atau lebih gen kunci yang menentukan proses tersebut. Keberhasilan mengidentifikasi gen-gen kunci akan membantu menyelesaikan masalah dalam regenerasi embrio kakao. Salah satu gen yang diduga terlibat dalam proses ini adalah AGAMOUS-like 15 (AGL-15). Gen ini berperan pada regulasi selama masa awal perkembangan embrio beberapa tanaman. Penelitian ini bertujuan untuk mengidentifikasi homolog AGL-15 pada kakao melalui pen-dekatan bioinformatika dan RT-PCR. Pene-litian diawali dengan identifikasi homolog AGL-15 dari DNA genomik daun kakao meng-gunakan primer heterologus. Sekuen fragmen homolog AGL-15 yang diperoleh, kemudian digunakan untuk merancang primer spesifik AGL-15 yang berukuran lebih panjang. Primer ini selanjutnya digunakan untuk meng-identifikasi gen AGL-15 dari RNA total embrio zigotik. Pengamatan pola pita gen AGL-15 dilakukan pada kultur in vitro embrio zigotik yang berumur lima minggu. Primer hetero-logus gen AGL-15 yang berasal dari berbagai tanaman, mampu mengidentifikasi keberadaan homolog gen tersebut pada tanaman kakao. Fragmen homolog AGL-15 putatif tanaman kakao teridentifikasi pada tingkat RNA embrio. Dengan adanya pola diferensial dari ekspresi gen AGL-15 hingga lima minggu pertama perkembangan embrio, ada indikasi kuat bahwa fragmen homolog AGL-15 dapat menjadi penanda embriogenesis pada tanaman kakao.

Optimasi pembuatan membran chitosam dalam penurunan COD dan BOD POME (Palm Oil Mill Effluent) [Optimization of the membrane production process to COD and BOD removal of POME (Palm Oil Mill Effluent)] Sri WAHYUNI; . SISWANTO; Alia DAMAYANTI
E-Journal Menara Perkebunan Vol 84, No 1: Oktober 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Palm oil is one of the main commodities that cultivated in Indonesia as the biggest palm oil producer. One of the main problems in palm oil industry is the difficulty to degradate the palm oil mill effluent (POME) due to the high quantity and content of COD and BOD. In physico-chemical, POME can be processed using membrane filtration technology. Chitosan is one of the most widely used material forproducing membrane filtration. Composite of Chitosan-PVA-PEG is a highly mixture absorbent, which possibly can be used as a membrane in filtration process of POME. The experiment was started with the production of composite membrane, and then filtration application using cross-flow reactor system. The variables of this experiment were chitosan and PVA ratio (3:7, 4:5, 1:1, 6:4 and 7:3 (v/v)), and stirring speed (100 rpm and 300 rpm). The reactor test was conducted for 50 minutes and permeate were taken every 10 minutes. Filtration output parameters that were analyzed flux, COD and BOD. The result showed that the highest flux values in the variation of the stirring speed of 100 rpm and 300 rpm were 40.20 L/m2.hr and 27.15 L/m2.hr, respectively. The highest rejection values of COD and BOD were obtained in membrane ratio variation of 1:1 (v/v) and stirring speed of 300 rpm, which are 97.24% and 97.60%, respectitively.

Peningkatan laju multiplikasi tunas dan keragaan planlet Stevia rebaudiana pada kultur in vitro Increasing shoot multiplication rate and plantlet vigor of Stevia rebaudiana in vitro culture . SUMARYONO; Masna Maya SINTA
E-Journal Menara Perkebunan Vol 79, No 2: Desember 2011
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractStevia (Stevia rebaudiana Bertoni) is a natural zero-calorie sweetener plant grown in a high population density.Tissue culture technique is useful for rapid mass propagationof plants to provide superior planting materials. Experimentswere conducted to increase growth and multiplication ofshoots and vigor of plantlets of stevia. Explants used wereapical and axillary buds from plantlets grown on MS mediumwithout plant growth regulators. Combinations of BA andIAA at different concentrations were used for shoot growthand multiplication, whereas plant growth retardants(ancymidol and paclobutrazol) and light intensity were usedfor plantlet vigor. The results showed that stevia explantscultured on MS medium without plant growth regulatorsproduced the highest shoots (4.5 cm) with two shoots perexplant. The best multiplication rate of shoots were found onMS medium added with 1.13 mg/L BA combined with0.35 mg/L IAA which produced on average 4.5 shoots and11.9 nodes per initial explant. Ancymidol and paclobutrazolconcentrations affected significantly growth and vigor ofstevia plantlets. Increasing the concentration of ancymidoland paclobutrazol decreased plantlet height and biomassfresh weight, but increased stem diameter. Paclobutrazol at0.1 mg/L was the best treatment to increase the vigor ofstevia plantlets. Light intensity at 20 µmol/m 2 /s gave betterplantlet vigor than other light intensities. It can be concludedthat multiplication of stevia shoots should be grown on MSmedium supplemented with 1.13 mg/L BA + 0.35 mg/L IAAand the vigor of the shoots can be increased by culturing onMS medium containing 0.1 mg/L paclobutrazol underfluorescence lamps with 20 µmol/m 2 /s light intensity.AbstrakStevia (Stevia rebaudiana Bertoni) adalah tanamanpemanis alami nir-kalori yang ditanam dengan kerapatanpopulasi yang sangat tinggi. Teknik kultur jaringan dapatdigunakan untuk perbanyakan tanaman secara massal dancepat untuk menyediakan bahan tanam unggul. Penelitiantelah dilakukan untuk meningkatkan pertumbuhan danmultiplikasi tunas dan keragaan planlet stevia. Eksplan yangdigunakan adalah tunas pucuk dan tunas samping dari planletyang ditumbuhkan pada medium MS tanpa zat pengaturtumbuh. Kombinasi BA dan IAA dengan konsentrasi yangberbeda digunakan untuk pertumbuhan dan multiplikasitunas, sedangkan zat penghambat tumbuh (ansimidol danpaklobutrazol) serta intensitas cahaya digunakan untukkeragaan planlet. Hasil penelitian menunjukkan bahwaeksplan stevia yang ditumbuhkan pada medium MS tanpa zatpengatur tumbuh menghasilkan tunas paling tinggi (4,5 cm)dengan dua tunas per eksplan. Multiplikasi tunas terbaikdiperoleh pada medium dengan BA 1,13 mg/L yangdikombinasikan dengan IAA 0,35 mg/L yang menghasilkan4,5 tunas dan 11,9 ruas per eksplan awal. Konsentrasiansimidol dan paklobutrazol berpengaruh nyata terhadappertumbuhan dan keragaan planlet stevia. Meningkatnyakonsentrasi ansimidol dan paklobutrazol menurunkan tinggiplanlet dan bobot basah biomassa, tetapi meningkatkandiameter batang. Paklobutrazol pada konsentrasi 0,1 mg/Lmerupakan perlakuan terbaik untuk meningkatkan keragaanplanlet stevia. Intensitas cahaya pada 20 µmol/m 2 /detikmemberikan keragaan planlet yang lebih baik dibandingkanintensitas cahaya yang lain. Dapat disimpulkan bahwamultiplikasi tunas stevia sebaiknya dilakukan pada mediumMS ditambah BA 1,13 mg/L + IAA 0,35 mg/L dan keragaanplanlet dapat ditingkatkan dengan menanam planlet padamedium MS ditambah paklobutrazol 0,1 mg/L di bawahlampu fluoresen dengan intensitas cahaya 20 µmol/m 2 /detik.

Emisi gas rumah kaca, cadangan karbon serta strategi adaptasi dan mitigasi pada perkebunan kopi rakyat di Nusa Tenggara Barat (Greenhouse gas emission, carbon stock, adaptation and mitigation strategies at smallholder coffee plantation in West Nusa Tenggara) Ali PRAMONO; . SADMAKA
E-Journal Menara Perkebunan Vol 86, No 2 (2018): Oktober 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Global warming and climate change are the world's major environmental, social and economic problems. The agricultural sector can act as an affected victim, greenhouse gas (GHG) contributor, and GHG absorber. Plantations have a very strategic role in the national action plan in GHG mitigation, because it has a great ability to absorb CO2. Therefore, it is necessary to determine the carbon stocks and GHG emissions from plantation management. The objectivesof the study wereto measure GHG emissions,to determine carbon stocks,and to define adaptation and mitigation strategies on climate change in existing coffee plantation systems. Gas samples were taken from 5 sampling points as replications by closedchamber method. Carbon stock estimation was done by destructive technique, including biomass of understorey and non-wood necromass. The results showed that the coffee plantations less than 10 years-oldat the study sites emitted 47 tons CO2-e/ha/year and stored carbon of 91.4 tons C/ha. Climate change adaptation strategies can be done by the application of good agricultural practices (GAP)andthe use of drought-tolerantclones, mulches,shade trees,multiple cropping systems, silt pitsand biophore techniques. The GHG mitigation can be done by the utilization of plantation waste as a source of organic fertilizer, biochar, animal feed, and bioenergy sources through the development of models of integration crop and livestock systems, as well as rejuvenation of plantation crops to increase carbon sinks and stocks. [Keywords: climate change, carbon sequestration, coffee plantations] Abstrak Pemanasan global dan perubahan iklim menjadi masalah utama lingkungan, sosial dan ekonomi dunia hingga saat ini. Sektor pertanian dapat berperan sebagai korban terdampak, penyumbang Gas Rumah Kaca (GRK), dan penyerapGRK. Tanaman perkebunan mempunyai posisi sangat strategis dalam rencana aksi nasional di sektor pertanian, karena memiliki kemampuan besar dalam menyerap CO2. Oleh karena itu, pengukuran cadangan karbon dan emisi GRK dari pengelolaan perkebunan perlu dilakukan. Tujuan penelitian adalah untuk mengukur emisi GRK, menentukan cadangan karbon dan menetapkan strategi adaptasi dan mitigasi terhadap perubahan iklim pada sistem perkebunan kopi rakyat eksisting di Propinsi Nusa Tenggara Barat. Pengambilan contoh gas dilakukan di limatitik sampling dengan metode sungkup tertutup. Penghitungan cadangan karbon di atas permukaan tanah dilakukan dengan cara destruktifyang mencakup juga penetapan cadangan karbon biomassatanaman bawah dan biomassaserasah (ne-kromas non kayu). Hasil penelitian menunjukkan bahwa perkebunan kopi rakyat yang berumur kurang dari 10 tahun di lokasi penelitianmeng-emisikan karbon sebesar 47 ton CO2-e/ha/tahundan menyimpan cadangankarbon sebesar 91,4ton C/ha. Strategi adaptasi terhadap perubahan iklim dapat dilakukan dengan penerapan praktik Pertanian yang baik/ Good Agricultural Practices(GAP), penggunaan klon tahan kekeringan, penggunaan mulsa organik, pemanfaatanpohon penaungdan sistem tumpang sari, pembuatan rorak dan biopori.Mitigasi GRK dapat dilakukan dengan pemanfaatan limbah tanaman perkebunan sebagai sumber pupukorganik, arang (biochar), pakan ternak, dan sumber bioenergimelalui pengem-bangan model sistem integrasi tanaman dan ternak,serta peremajaan tanaman perkebunan yang sudah menurun produktivitasnya untuk meningkatkan serapan dan cadangankarbon. [Kata kunci :perubahan iklim, sekuestrasi karbon, perkebunan kopi]

Overexpression of chitinase gene with a GC-rich synthetic enhancer in tobacco plant (Nicotiana tabacum L.) Overekspresi gen kitinase dengan enhancer sintetis kaya GC pada tanaman tembakau (Nicotiana tabacum L.) D SANTOSO; T CHAIDAMSARI; A BUDIANI; H MINARSIH; S DWI UTOMO; . SISWANTO
E-Journal Menara Perkebunan Vol 68, No 2: Desember 2000
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Ringkasan Perakitan tanaman perkebunan toleran terhadap serangan cendawan patogenik dilakukan dengan mengoverekspresikan gen penyandi kitinase. Untuk itu elemen DNA peningkat ekspresi (enhancer E52) yang berupa oligonukleotida 52 pb dan kaya kandungan basa purin (GC) disisipkan di ujung 5’ konstruk 35S-chi. Penyisipan E52 tersebut dilakukan secara lebih terarah pada situs ganda HindIII-SalI dari MCS pCAMBIA2301. Melalui situs HindIII yang terletak tepat di ujung 3’ E52, konstruk 35S-chi kemudian disambungkan dengan E52 pada pCAMBIA tersebut. Transformasi DNA rekombinan ke dalam sel tembakau dikerjakan melalui perantaraan Agrobacterium tumefaciens LBA4404. Sel tanaman transgenik diseleksi dan diregenrasi dalam media yang mengandung 3% sukrosa, 0,5 mg/L diregenerasikan pada media MS padat benzilaminopurin (BAP) dan 50 mg/L kanamisin. Pada media ini tunas transgenik tembakau mulai terbentuk setelah 5 minggu penanaman. Analisis tingkat aktivitas enzimatis menunjukkan bahwa aktivitas kitinase pada tembakau transgenik 40 hingga 80 kali lebih tinggi daripada non-transgenik. Pengujian hibridisasi protein menggunakan antibodi anti-kitinase, dot blot dan western blot, membuktikan bahwa enhancer tersebut dapat meningkatkan ekspresi transgen chi pada tanaman tembakau.Summary Development of estate crops tolerant to pathogenic fungi is conducted by overexpressing chi gene. For this purpose, a synthetic enhancer consisting of 52 base pairs and GC-rich was inserted at immediate 5’ end of a 35S-chi cassette. Insertion of the E52 was directed at HindIII-SalI restriction sites of the pCAMBIA2301 MCS. With HindIII restriction site located just after the 3’ end of the E52 sequence, the 35S-chi construct was then ligated with the E52 of the pCAMBIA. Transformation of the resulting recombinant DNA into tobacco cells was mediated by Agrobacterium tumefaciens LBA4404. The transgenic cells were selected and regenerated on a solid MS medium supplemented with 3% sucrose, 0.5 mg/L benzylamino purine (BAP) and 50 mg/L kanamycin. Tobacco shoots were initiated after 5 weeks inoculation on the selection media. Enzymatic analysis demonstrated that chitinase activity of transgenic tobacco was 40 to 80 folds higher than that of the control plant. Analysis of enzymatic activity using hybridization with anti-chitinase antibody indicated that the level of chitinase activity in the transgenic tobacco carrying the enhancer is higher than that without enhancer. These data suggest that the enhancer improved the expression of chi transgene in tobacco.

Identifikasi dan pencegahan kontaminasi pada kultur cair sistem perendaman sesaat Identification and prevention of contamination in liquid culture of temporary immersion system Masna Maya SINTA; Imron RIYADI; . SUMARYONO
E-Journal Menara Perkebunan Vol 82, No 2: Desember 2014
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractLiquid culture is commonly used to scale up in vitro culture production as well as to optimize the developmental phase of plant in vitro culture. One of the liquid cultures that has been used widely is temporary immersion system (TIS). The main problem of liquid culture is contamination. The use of antibiotics sometimes controls the contaminants less effectively and hinders the growth of plant culture. The purpose of this research was to determine sources of contaminant on whole sequence of TIS to identify and to prevent the emergence of the contaminants. Sampling method was applied to each section and stage of TIS culture and the contaminants found were identified. The results revealed that compartment of TIS was the main source of contaminant (100%). Furthermore, from all components of TIS compartment, washer (a small ring seal connecting screen disc and basket) was the main source of TIS contaminant (41.2%). Four contaminants found were identified as Bacillus macerans, Bacillus megaterium, Bacillus sphaericus and Bacillus firmus. Two times sterilization of washer in an autoclave at temperature of 121 oC and air pressure of 1 kg/cm2 for 20 minutes before and after being installed reduced the contamination level on TIS culture significantly.AbstrakKultur cair umumnya digunakan untuk meningkatkan skala produksi dan mengoptimalkan fase perkembangan kultur in vitro tanaman. Salah satu jenis kultur cair yang banyak digunakan adalah sistem perendaman sesaat (SPS). Masalah utama dalam kultur cair adalah kontaminasi. Penggunaan antibiotika terkadang kurang efektif dalam me-ngendalikan kontaminan dan menghambat pertumbuhan kultur tanaman. Tujuan dari penelitian ini adalah untuk mengetahui sumber kontaminan pada seluruh rangkaian kultur SPS serta mengidentifikasi dan mencegah munculnya kontaminan tersebut. Metode yang digunakan adalah pengambilan contoh pada tiap bagian dan fase kultur SPS, serta kontaminan yang ditemukan kemudian diidentifikasi. Hasil penelitian memperlihatkan bahwa kompartemen SPS merupakan sumber utama kontaminan (100%). Selanjutnya, dari seluruh komponen kompartemen SPS, washer (cincin penutup yang menghubungkan penyaring dan keranjang) di dalam rangkaian SPS merupakan sumber utama kontaminan (41,2%). Empat kontaminan yang ditemukan diidentifikasi sebagai Bacillus macerans, Bacillus megaterium, Bacillus sphaericus dan Bacillus firmus. Sterilisasi cincin penutup sebanyak dua kali dalam autoklaf pada suhu 121 oC dan tekanan udara 1 kg/cm2selama 20 menit sebelum dan sesudah dirangkai secara nyata menurunkan tingkat konta-minasi pada kultur SPS.

Arsitektur akar bibit kelapa sawit yang diinokulasi beberapa cendawan mikoriza arbuskula Root architecture of oil palm seedling inoculated with selected arbuscular mycorrhizal fungi Happy WIDIASTUTI; Edi GUHARDJA; Nampiah SUKARNO; Latifah KOSIM DARUSMAN; Didiek Hadjar GOENADI; Sally SMITH
E-Journal Menara Perkebunan Vol 71, No 1: Juni 2003
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary Oil palm is mostly cultivated in acid soil. The growth constraint of plant in acid soil is the limited availability of phosphorus (P) nutrient. Improvement of root system morphology and architecture have an important aspect since P is immobilized nutrient. Colonization of oil palm by rrbuscular mycorrhizal fungi increase the P uptake of plant. However, there is no information related to the effect of AM fungal colonization on oil palm root morphology and architecture. A research has been conducted to asses the effect of colonization of two species of AM fungi on root system morphology and architecture of oil palm seedling. The research was conducted using Cikopomayak acid soil as medium in simple glass chamber. The plant material was from Indonesian Oil Palm Research Institute, Medan while AM fungal inoculum was produced using pot culture. Six treatments assesed are combination of three levels of AM fungi inoculation (without inoculation with, Acaulospora tuberculata and Gigaspora margarita) and two levels of fertilization (without, and with fertilizer). The result showed that colonization of AM fungi could change the root system morphology, and root architecture. The root fresh weight, root dry weight, length, and volume were significantly higher with the AM fungi colonization especially A. tuberculata inoculation. However, specific root weight was not significantly different between inoculated and uninoculated. The enhancement was significantly observed 26 weeks after inoculation. Biside that, proportion of secondary root of oil palm inoculated with AM fungi was higher compared to primary root. Fertilization tend to reduced root growth. Fertilization reduced significantly root shoot ratio of inoculated as well as uninoculated seedlings. The rooting volume was higher in inoculated seedling compared to uninoculated. The highest enhancement of N, P, and K uptake was observed 26 weeks after inoculation. The better root morphology and architecture might be one mechanisms of AM fungi colonized oil palm seedlings in increasing P uptake. Ringkasan Umumnya tanaman kelapa sawit ditanam pada tanah masam. Hambatan pertumbuhan tanaman pada tanah masam adalah terbatasnya ketersediaan nutrisi P (fosforus). Oleh sebab itu perbaikan sistem morfologi dan arsitektur akar memiliki aspek yang penting disebabkan P merupakan nutrisi yang tidak mudah bergerak. Kolonisasi tanaman kelapa sawit dengan cendawan mikoriza arbuskula (CMA) akan meningkatkan penyerapan P oleh tanaman. Namun, hubungan antara simbiosis CMA dengan arsitektur perakaran kelapa sawit belum diketahui. Penelitian ini dilakukan untuk mempelajari pengaruh kolonisasi dua spesies CMA pada sistem morfologi dan arsitektur akar bibit tanaman kelapa sawit. Percobaan dilakukan menggunakan tanah masam Cikopomayak yang mengandung Al tinggi sebagai medium dalam kultur pot kaca yang sederhana. Kecambah kelapa sawit berasal dari Pusat Penelitian Kelapa Sawit (PPKS), Medan, sedangkan inokulum CMA diproduksi menggunakan kultur pot. Enam perlakuan yang diuji merupakan kombinasi tiga jenis inokulasi CMA ( tanpa inokulasi, inokulasi dengan Acaulospora tuberculata dan Gigaspora margarita) serta dua tingkat pemupukan (tanpa, dan dengan pemupukan). Hasil yang diperoleh menunjukkan bahwa inokulasi CMA merubah sistem morfologi dan arsitektur perakaran. Bobot basah, bobot kering, panjang dan volume akar nyata lebih tinggi pada tanaman yang dikolonisasi CMA khususnya A. tuberculata. Namun berat akar spesifik tidak beda nyata antara yang diinokulasi dan tanpa inokulasi. Peningkatan berat akar sangat nyata setelah 26 hari diinokulasi. Di samping itu proporsi akar sekunder lebih tinggi dibandingkan dengan akar primer pada tanaman kelapa sawit yang diinokulasi CMA. Pemupukan pada umumnya menurunkan pertumbuhan akar dan secara nyata menurunkan nisbah akar pucuk. Volume perakaran lebih besar pada bibit kelapa sawit yang diinokulasi dibandingkan dengan yang tidak diinokulasi. Peningkatan serapan N, P, dan K tertinggi teramati 26 minggu setelah inokulasi. Morfologi perakaran yang lebih baik demikian pula arsitektur perakaran mungkin merupakan mekanisme bibit kelapa sawit bermikoriza dalam meningkatkan serapan P.

Aplikasi biokaolin untuk perlindungan buah kakao dari serangan PBK, Helopeltis spp. dan Phytophthora palmivora Application of biokaolin in protecting cocoa pod from cocoa pod borer, Helopeltis spp. and Phytophthora palmivora infestation Irma KRESNAWATY; Asmini BUDIANI; Abdul WAHAB; T W DARMONO
E-Journal Menara Perkebunan Vol 78, No 1: Juni 2010
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractMain constraints of cacao cultivation are infestations of cocoa pod borer (Conopomorpha cramerella (Snellen), Helopeltis spp., and cocoa pod rot disease (palmivora). So far there is no technology that could efficiently controlthese important pests. This research was aimed to develop environmentally friendly new technology to protect pod surfaces of cacao. The experiment was performed in heavily infested cacao plantation in Konawe, South-East Sulawesi.The use of kaolin particle film enriched with entomopathogenic microbe was contrasted againts the use of currently recommended plastic sleeve. Cacao pods were sprayed at one week and two week intervals. The observed parameters were the number of pods infested with cocoa pod borer, pod rot and Helopeltis spp. at 4th - 14th weeks after first spray. From the observation, weekly biokaolin application showed the highest amount pods free from cocoa pod borer (33.97 %), followed by biweekly application (27.96 %), and plastic sleeving (19 %). Ten weeks after first spray, cocoa pod borer incidence was significantly reduced especially in weekly application. The percentage of pods free from pod rot were 81.92 %, 62.96 %, and 72.20 % for weekly spray, biweekly spray, and plastic sleeving, respectively. Pods being kept for 12 weeks in plastic sleeve endured the highest intensity of pod rot incidence. Biweekly biokaolin treatment was better in handling Helopeltis spp. attack. Besides reducing infestation of the main pests and diseases, biokaolin application also reduced the incidence of cherelle wilt to almost 40%. Those results gave the great expectation that biokaolin usage would significantly increase cacao yield, resulting in the increase of cacao farmer income. AbstrakKendala utama dalam upaya budidaya kakao adalah adanya serangan hama penggerek buah kakao (PBK), Conopomorpha cramerella (Snellen) dan hama kepik Helopeltis spp., serta serangan patogen penyebab busuk buah (Phythophtora palmivora). Sampai saat ini belum tersedia teknologi yang secara efisien mengendalikan ketiga-tiganya sekaligus. Peneltian ini bertujuan untuk mengembangkan teknologi yang ramah lingkungan untuk melindungi permukaan buah kakao. Percobaan dilakukan pada perkebunan kakao dengan tingkat serangan yang berat di Konawe, Sulawesi Tenggara. Dalam penelitian ini, penggunaan lapisan partikel kaolin yang diperkaya dengan mikroba entomopatogenik dibandingkan efektifitasnya dengan penyarungan menggunakan kantung plastik yang direkomendasikan selama ini. Buah kakao disemprot setiap interval satu minggu dan dua minggu sekali. Parameter yang diamati adalah jumlah buah terserang PBK, jumlah serangan busuk buah dan jumlah serangan Helopeltis spp. pada minggu keempat sampai dengan minggu ke-14 setelah penyemprotan pertama. Hasil pengamatan menunjukkan bahwa persentase tertinggi (33,97 %) buah kakao yang terbebas dari serangan PBK diperoleh pada plot dengan penyemprotan biokaolin setiap minggu, diikuti dengan penyemprotan setiap dua minggu (27,96 %), dan penyelubungan dengan kantung plastik (19,00 %). Pada minggu ke- 10 setelah penyemprotan pertama terjadi penurunan intensitas serangan PBK secara signifikan khususnya pada perlakuan setiap minggu. Persentase buah kakao yang terbebas dari penyakit busuk buah 81,92 %, 62,96 %, dan 72,20 %, secara berturutan untuk perlakuan penyemprotan setiap satu minggu,setiap dua minggu, dan penyarungan plastik. Pada minggu ke-12 buah kakao yang diberi perlakuan penyarungan mengalami peningkatan serangan busuk buah paling tinggi dibandingkan dengan perlakuan lainnya. Perlakuan penyemprotan setiap dua minggu memberikan perlindungan terbaik dari serangan Helopeltis spp. Hasil ini memberikan harapan besar bahwa aplikasi biokaolin sangat berpotensi meningkatan hasil panen petani kakao sehingga akan meningkatkan pendapatan petani.

Aktivitas ligninolitik Omphalina sp. hasil isolasi dari TKKS dan aplikasinya untuk dekolorisasi limbah kosmetik Ligninolytic activity of Omphalina sp. isolated from EFB and its application for decolorization of cosmetic waste . SUHARYANTO; Irma KRESNAWATY; Haryo Tejo PRAKOSO; Deden Dewantara ERIS
E-Journal Menara Perkebunan Vol 80, No 2: Desember 2012
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Abstract White-rot fungi (WRF) are belong to Basidiomycetes group that capable to degrade lignin, because they produce extracelullar ligninolytic enzymes such as lignin peroxsidase (Li-P), mangan peroxidase (Mn-P) and laccase. The ligninolytic activity can be used in bioprocess oxidation system such as biopulping, biobleaching and bioremediation. The purposes of this research were to determine the optimum conditions of growth and ligni-nolytic activity of Omphalina and to observe its potential to decolorize cosmetics wastewater. Omphalina sp. was grown on media of PDA-Remazol Brilliant Blue R (RBBR) and PDA-Guaiacol (GU) at various pH and temperature conditions. The decolorization of cosmetic effluent was conducted by applying Omphalina sp. at various dose of inoculum. Decolorization rate and change of COD were observed for eight days. The results showed that Ompha-lina sp. could grow and produce peroxidase enzyme both on RBBR and GU media at pH 4.5-8.5 and temperature 23-350C. Optimum dose of inoculum was as much as 5% w/v at which the fungus was able to decolorize cosmetic factory effluent up to 92.79% and to decrease COD value up to 48.57 % after eight days of incubation.Abstrak Jamur pelapuk putih (JPP) merupakan jamur kelompok Basidiomycetes yang mampu mendegradasi lignin karena memproduksi enzim-enzim ligninolitik ekstraseluler seperti lignin peroksidase (Li-P), mangan peroksidase (Mn-P) dan lakase. Kemampuan ligninolitik JPP dapat dimanfaatkan dalam sistem oksidasi bioproses seperti biopulping, biobleaching dan bioremediasi. Pene-litian bertujuan menetapkan kondisi optimum pertumbuhan Omphalina sp. dan aktivitas ligninolitik yang dihasilkan-nya serta mempelajari potensinya dalam mendekolorisasi limbah cair kosmetik. Omphalina sp. ditumbuhkan dalam media PDA-Remazol Brilliant Blue R (RBBR) dan PDA-Guaiakol (GU) pada berbagai variasi pH dan suhu. Percobaan dekolorisasi limbah cair kosmetik dilakukan dengan aplikasi inokulum dalam berbagai dosis. Laju dekolorisasi dan perubahan COD diamati selama delapan hari. Hasil penelitian menunjukkan Omphalina sp. tumbuh dan menghasilkan enzim peroksidase, baik pada media RBBR maupun GU pada pH 4,5-8,5 dan suhu 25-350C. Dosis optimum aplikasi Omphalina sp. adalah 5% (b/v) yang mampu mendekolorisasi limbah cair pabrik kosmetik hingga 92,79% dan menurunkan COD 48,57% setelah delapan hari.

Pengaruh periode perendaman air dan komposisi media tumbuh terhadap keberhasilan aklimatisasi planlet sagu (Effect of water immersion period and growing media composition on acclimatization success of sago palm plantlets ) . SUMARYONO; Imron RIYADI
E-Journal Menara Perkebunan Vol 85, No 2 (2017): Oktober 2017
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Sago palm (Metroxylon sagu Rottb.) is a carbohydrate-producing crop, commonly propagated by suckers. The availability of planting materials in a large quantity hinders the development of commercial sago plantations. Sago propagation by tissue culture via somatic embryogenesis has been developed to provide superior planting materials of sago palm. One of the major problems faced in tissue culture of sago palm is the low survival rate of plantlets during acclimatization period. The objective of this research was to increase the acclimatization success of sago plantlets in term of survival rate and growth at ex vitro conditions. The experiments were conducted using a randomized block design with two factors i.e. water immersion period and growing media composition. The water immersion periods used were without immersion, immersion for 2 days with 1 day intermittent period, immersion for 1 day with 1 day intermittent period, immersion for 1 day with 2 days intermittent period, and continuous immersion. The growing media used were consisted of top soil, sand, dung manure, and cocopeat at different compositions. Sago plantlets were planted on small plastic pots and placed inside a closed plastic tunnel for 12 weeks. Research results showed that continuous water immersion and mixed composition of soil, sand, and cocopeat (1:1:2 v/v) was the best conditions for acclimatization of sago plantlets with the survival rate of 70% after 12 weeks. The survived plants had good leaves and roots, ready to be transferred to big plastic bags in the main nursery. [Keywords: Metroxylon sagu, sago palm, acclimatization, immersion, media composition] AbstrakTanaman palma sagu (Metroxylon sagu Rottb.) termasuk tanaman penghasil karbohidrat yang umumnya diperbanyak dengan anakan (suckers). Ketersediaan bahan tanam dalam jumlah besar merupakan hambatan pengembangan perkebunan sagu komersial. Kultur jaringan tanaman sagu telah dikembangkan melalui teknik embriogenesis somatik untuk memenuhi kebutuhan bahan tanam unggul sagu. Salah satu masalah utama dalam kultur jaringan sagu adalah rendahnya daya hidup planlet pada tahap aklimatisasi. Penelitian ini bertujuan meningkatkan keberhasilan aklimatisasi planlet sagu yang meliputi daya hidup dan pertumbuhan bibit pada lingkungan ex vitro. Percobaan dilakukan menggunakan rancangan acak kelompok dengan dua faktor yaitu perendaman air dan komposisi media. Perlakuan perendaman air adalah tanpa perendaman, perendaman 2 hari diselang tanpa perendaman 1 hari, perendaman 1 hari diselang tanpa perendaman 1 hari, perendaman 1 hari diselang tanpa perendaman 2 hari, dan perendaman terus menerus. Komposisi media tumbuh yang digunakan berupa perbandingan volume penyusun yaitu tanah, pasir, pupuk kandang dan cocopeat. Planlet ditanam di pot kecil dan diletakkan di dalam sungkup plastik tertutup selama 12 minggu. Hasil penelitian menunjukkan bahwa perlakuan perendaman terus menerus dan media tumbuh campuran tanah, pasir, cocopeat (1:1:2 v/v) merupakan kondisi terbaik pada aklimatisasi planlet sagu dengan daya hidup mencapai 70% setelah 12 minggu. Bibit yang dihasilkan memiliki daun dan perakaran yang baik, siap untuk dipindahkan ke pot plastik besar di persemaian utama.[Kata kunci: Metroxylon sagu, sagu, aklimatisasi, perendaman, komposisi media]

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Hayati Minarsih

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Home Page

OAI Link

Editorial Team

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Reviewer

Google Scholar

Contact Name Hayati Minarsih

Contact Email menaraperkebunanppbbi@gmail.org

Phone -

Journal Mail Official menaraperkebunan@iribb.org

Editorial Address Jalan Taman Kencana No.1 Bogor 16128, Jawa Barat

Location Kab. bogor, Jawa barat INDONESIA

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Contact Name
Hayati Minarsih

Contact Email
menaraperkebunanppbbi@gmail.org

Phone
-

Journal Mail Official
menaraperkebunan@iribb.org

Editorial Address
Jalan Taman Kencana No.1 Bogor 16128, Jawa Barat

Location
Kab. bogor,

Jawa barat

INDONESIA