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Jurnal Analis Medika Biosains (JAMBS)
Published by Poltekkes Kemenkes Mataram
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
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Articles 313 Documents
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Analisis Motivasi Pendonor Darah di PMI Kabupaten Sleman Yogyakarta Hartalina Mufidah; Handriani Kristanti; Eva Runi Khristiani
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.272

Abstract

Blood donation is an essential element in blood transfusion. The Indonesian Red Cross of Sleman Regency (PMI) is one of the Blood Donor Units in the Province of D.I. Yogyakarta, but only 1% of the population in Sleman became blood donors. Blood donation motivation is closely related with knowledge, attitude, and behavior. This study aims to determine the motivational factors for blood donation at PMI Sleman Regency. This research used a cross-sectional study design which was carried out at PMI Sleman in January-December 2021. The sample of this study was 100 respondents who were determined using the accidental sampling technique. Analysis of research data was carried out univariate and bivariate with Fisher's exact test. The results of this study indicate that age (p-value 0.901>0.05), gender (p-value 0.597>0.05), education (p-value 0.914>0.05), occupation (p-value 0.797>0.05), knowledge (p-value 0.097>0.05), and attitudes (p-value 0.393>0.05) didn’t have relationship with blood donor motivation, but there was relationship between behavior and motivation about blood donation with p-value 0.009<0 ,05.  Based on the results of this study, it can be concluded that knowledge, attitudes, and behavior are motivational factors for blood donors, but behavior is a significant factor in blood donor motivation in PMI, Sleman Regency, Yogyakarta. Increasing routine blood donation activities and educating the public about the value of blood donation could create good behavior for voluntary blood donors
Effect of Soaking Time’s Variation of Red Onion (Allium Cepa L) Extracts on Formalin Content in Mackerel Tuna (Euthynnus Affinis) Indah Denissa Lestyaninrum; Kurnia Ritma Dhanti; Retno Sulistiyowati; Kurniawan Kurniawan
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.289

Abstract

Mackerel tuna (Euthynnus affinis) is one of the most popular foods in the community since it is affordable and has complete nutritional content. It is a frozen fish food product so the storage period of which is relatively short. In addition, it is possible to add formalin on which. Formalin is a substance that is prohibited from being used due to its dangerous effects. The content of saponin compounds found in red onions (Allium cepa L) can be used to reduce formalin levels. This study aimed to determine the effect of variations in the soaking time of red onion extract on the formalin level in mackerel tuna and the optimal soaking time for reducing formalin levels. This study used an experimental method with a posttest control group design. The treatment was given by soaking red onion extract at a concentration of 10% in mackerel tuna containing formalin with variations in soaking time of 1 hour, 2 hours, and 3 hours which were tested quantitatively using a UV-Vis spectrophotometer. Then, the data were analyzed using a one-way ANOVA statistical parametric test and a post hoc test using Duncan's test (DMRT). The decrease of the formalin levels by soaking the onion extract for 1 hour, 2 hours, and 3 hours was 10.61%, 41%, and 73%, respectively. The result showed that there was an effect of the soaking time of red onion extract on formalin levels (p-value 0.05), and the optimal time of soaking onion extract to reduce the formalin content of the samples was 3 hours.
The Antibacterial Activity of Dayak Onion Ethanol Extract (Eleutherine Palmifolia (L.) Merr) and Red Ginger (Zingiber Officinale Rosc Var. Rubrum) on Growth Gi Tract Pathogen Bacteria Tiara Dini Harlita; Ganea Qorry Aina
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.299

Abstract

Antibiotic resistance in gastrointestinal bacterial infections can be overcome by alternatives using medicinal plants such as Dayak bulbs (Eleutherine palmifolia (L.) Merr) and red ginger rhizome (Zingiber officinale Rosc. Var. Rubrum). This study aims to examine the antibacterial activity of a combination of ethanol extracts of Dayak bulbs with red ginger rhizomes and the effectiveness of the extract combinations in inhibiting the growth of Pseudomonas sp. and Bacillus sp. This research is an experimental study with a completely randomized design (CRD) of one factor, namely a combination of ethanol extract of Dayak onion tubers and red ginger rhizomes with 4 variations of concentration 25, 50, 75, and 100 mg/mL at 2 ratios of the combination of 2:1 and 3:1 for 48 experimental units. Tests were carried out on bacteria Pseudomonas sp. and Bacillus sp. in vitro with agar diffusion technique (Kirby Bauer test), while the positive control was Chloramphenicol 30 μg / mL and the negative controls were sterile aquadest. Data analysis in this study used the ANOVA test. The results showed ethanol extract of dayak onion bulbs, red ginger rhizomes, and a combination of the two extracts have antibacterial activity so which can be inhibiting the growth of Pseudomonas sp. and Bacillus sp. The combination of ethanol extract from dayak onion bulbs and has the greatest antibacterial activity at a 3:1 combination of 56.95% against the growth of Pseudomonas sp. and 109.72% against the growth of Bacillus sp.
Comparison of Carboxyhemoglobin (COHb) Levels in the Blood of Active and Passive Smokers in Bulupitu Bus Terminal Workers, Banyumas Regency Noviani Nur Hayati; Kurnia Ritma Dhanti; Retno Sulistiyowati; Tantri Analisawati Sudarsono
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.290

Abstract

Carbon monoxide (CO) is a colorless, tasteless, and odorless gas, but in high concentrations in the blood, it can cause death. One of the CO2 can come from cigarette smoke. When cigarette smoke is inhaled, the affinity of CO with the blood is stronger than for oxygen, and this will increase the carboxyhemoglobin (HbCO) in the blood. Passive smokers who are often near active smokers will inhale cigarette smoke. This study aims to compare HbCO levels in the blood between active and passive smokers of Bulupitu terminal workers, Banyumas Regency. This was an analytical observational study with six active and six passive smokers who are terminal workers. All active and passive smokers samples had HbCO concentrations above the normal HbCO threshold of 3.5%. The measurement results showed the average HbCO level of smokers was 5.87%. Determining differences in HbCO levels in the blood of active and passive smokers was conducted using the Independent Test (p-value = 0.370). This value shows no significant difference in blood HbCO levels in active and passive smokers. 
Description of Uric Acid Levels and Blood Pressure In Pulmonary Tuberculosis Patients Consuming Anti Tuberculosis Drug (ATD) in the Working Area of Sakra Public Health Center Nizar Wahyudi; I Wayan Getas; Ida Bagus Wiadnya; Lalu Srigede
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.302

Abstract

Background: Anti-tuberculosis (ATD) used is the standard blend of INH, PAS and streptomycin for one to two years. The amino salylic (pas) acids are then replaced with pirazinacids. Pirazinbacteria is a drug that can cause increased levels of uric acid in the body (hyacinth). Hiperurisemia is the state of concentration of the veins in the plasma or the serum has exceeded the limit of the monosodium ligament of 7.0 milligrams /dl. Hyperuricemia is also cited as an important risk factor for hypertension and other cardiovascular diseases. Research purposes: For details of uric acid and blood pressure on lung tuberculosis patients who use antituberculosis (oats) in the region of the sakra hospital. Research methods: The kind of research used I s a descriptive observation study with a sectional design, a study in which dependent and independent variables are done and measured simultaneously. The sample retrieval technique on this study USES a total sample using the criteria the researchers have chosen in selecting a sample. Research: Average uric acid and blood pressure in lung tuberculosis patients who consume single-month intensive drugs (ATD) are 7,8 mg/dl and 121/75 mmHg. Average levels of uric acid and blood pressure in lung tuberculosis patients who use 2 months of intensive phase (ATD) are 9,1 mg/dl and 129/78 mmHg. Conclusion: There are high levels of uric acid and blood pressure in those with lung TB who take anti-tuberculosis drugs (ATD).
Differences in Results of Examination of Urine Protein Levels Dipstik Method with 6% Acetic Acid Method in Patients with Diabetes Mellitus in Area Puskesmas Baturraden II Feni Putri Rahayu; Retno , Sulistiyowati; Tantri Analisawati Sudarsono; Minto Rahaju
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.291

Abstract

Diabetes Mellitus (DM) is a non-communicable disease and a major public health issue in Indonesia and around the world. Proteinuria caused by diabetes in the blood vessels can cause kidney damage. Diabetes can also cause nerve damage in the body. This can make urinating difficult. The pressure caused by a full bladder can harm the kidneys. If urine remains in the bladder for a longer duration, it can cause an infection due to the rapid growth of bacteria in urine with a high sugar level, even if the presence of protein in the urine has been tested in a laboratory. Objective: The study aimed to determine the difference in the results of urine protein examination with the dipstick method with the 6% acetic acid method in DM patients in the working area of Puskesmas (Public Health Center) Baturraden II. Methods: The research design used was analytic observational with a cross-sectional study. Thirty urine samples were used with purposive sampling as the sampling technique. Chi-Square Test was employed for Statistical Analysis. The results of the tests produced a significant value of 0.000 (P 0,0 5), indicating that the p-value was lower in relation to the degree of error. It could be concluded that the results of urine protein examination using the dipstick method and the 6% acetic acid method differed in DM patients.
Morphological Analysis and Biochemical Properties of Staphylococcus aureus Cultures Grown on Alternative Media Peanut Flour (Arachis Hypogaea L.) Yudha Anggit Jiwantoro; Salmania Putri
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.305

Abstract

Sources of nutrition especially sufficient protein, is needed for the growth of Staphylococcus aureus bacteria. Synthetic media is the most common medium for growing Staphylococcus aureus. Peanut is a biological source in nature with a higher protein content than synthetic materials which can be used as an alternative. Objective to determine the morphology and biochemical properties of Staphylococcus aureus bacteria grown on alternative media of peanut flour (Arachis hypogaea L.) as a medium for bacterial growth. This is a True-Experiment study with a completely randomized design. The experimental unit consists of five variations in the concentration of peanut alternative media. The sampling technique was done by simple random sampling. Data analysis used Descriptive. Staphylococcus aureus bacteria can grow at all concentrations of alternative media of peanut flour. The colony size at concentration of 2% was very small with weakly fermented mannitol. Meanwhile a concentration of 3% to 5% produces moderately fermented mannitol. The concentration of 6% is the concentration with the most widely fermented mannitol. The biochemical tests of all concentrations yielded positive catalase and coagulase, and the sugar test yielded positive reactions. An alternative medium of peanut flour can be used as a medium for the growth of Staphylococcus aureus bacteria with the most effective concentration of 6%.
Quantity Evaluation of Laactic Acid Bacteria Using the Fncc 0027 and C410li Gene on Different Storage of Acid Bamboo Putri Widelia Welkriana; Halimatussa&#039;diah Halimatussa&#039;diah
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.296

Abstract

The fiber content in bamboo shoots is higher than other tropical vegetables. The water content in saw bamboo shoots is quite high, causing the bamboo shoots to have a shelf life of only 2 days. The fermentation process is characterized by the conversion of carbohydrates into lactic acid by bacteria. This study aimed to calculate LAB with FNCC 0027 and C410LI genes in tamarind shoots with different storage periods. The method used in this study is a descriptive method to describe the total plate number of microbes, LAB examination and molecular identification of bacteria found in tamarind shoots with different storage times. The results showed that all samples showed the presence of microbial TPC, examination. As for molecular identification, it was found that samples 1, 2, and 3 were identified as LAB with the C4101LI gene, while the FNCC0027 gene was not detected. Concluded that the total plate number values with different storage times of tamarind shoots, namely 0, 24, and 48 hours were 2.5x105 CFU/g, 5.4x105 CFU/g, and 7.3x105 CFU/g. In samples 1, 2, and 3, there were lactic acid bacteria of the type Bacillus plantarum C401L1.
Effects of Carbohydrate Diet Programs on Urine Ketones Positiveness with Long Time on a Diet 1 Year, 2 Years and 3 Years Dyah Astining Maya; Iswari Pauzi; Siti Zaetun; Yudha Anggit Jiwantoro
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.303

Abstract

Carbohydrate diet is a diet pattern that reduces carbohydrate consumption. Therefore, the body will use fat as an alternative energy source. The continuous use of fat causes the accumulation of fatty acids so that it often causes ketone positivity in the urine. Objective : Find out the effect of a carbohydrate diet program on the positivity of urine ketones. Method : This research is an analytic observational study with a cross sectional approach. The sample used was urine from respondents who had been on a carbohydrate diet for 1 year, 2 years and 3 years, then urine ketones were examined using the dipstick method. Data analysis was carried out using the Chi-Square test with a confidence value (α = 0.05). Result : The sample in this study amounted to 45 samples, with a total of 23 samples of positive ketones and 22 samples of negative ketones. The results of the Chi Square test obtained an Asymp. sig value of 0.009 <0.05 (α) which indicates that the carbohydrate diet program has a significant effect on the positivity of urine ketones.
Lemongrass Powder in Bio-Bs Effervescent Formulation of Lombok Island Local Isolate on Viability and Amount of Bacillus Sphaericus Toxin Crystal Protein for Control of Anopheles sp. Larvae Yunan Jiwintarum; Zainal Fikri; Maruni Wiwin Diarti; Rabi&#039;unnisa Sulaimah
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.266

Abstract

Bio Formula – BS effervescent (Bio- Bacillus sphaericus) is an Effervescent powder-shaped formula that is easy to use by the public. Lombok's local effervescent bio-BS isolated formula has a weakness in terms of smell. The results from the literature search that fragrant lemongrass has a distinctive smell and can kill Aedes aeygpti, but scientific data has not been obtained about the ability of fragrant lemongrass to kill Anopheles Sp in the form of larvae and mosquitoes. The study aimed to find out the effect of the combination formulation of Bio-BS Effervescent local isolate Lombok Island with the addition of lemongrass powder for viability and the amount of toxin crystal protein Bacillus sphaericus. This research method is exploratory and experimental in the laboratory with the design of Post Test Only Control Group Design. The study used six treatment formulations. Samples of Anopheles sp larvae research in Batu Layar lagoon, West Lombok regency and pelur lagoon in Peringgesela, East Lombok regency and from the results of colonization of larvae. The independent Variable is a combination formulation of Bio-BS Effervescent isolated locally from Lombok Island with the addition of fragrant lemongrass powder. The dependent variable is the mortality of Larvae Anophels Sp, Viability of B. Sphaericus, and Amount of Toxin Protein Production of B. Sphaericus. The larvae death rate, the concentration of cells/endospores, and the number of repeats in each container are then tabulated and analyzed using Probit Analysis with the help of MINITAB 16 software. B. sphaericus viability data and the amount of endospore toxin protein crystal production were descriptively analyzed.

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