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Dwi Anggorowati Rahayu
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Jurnal Riset Biologi dan Aplikasinya
Published by Universitas Negeri Surabaya
ISSN : -     EISSN : 26559927     DOI : 10.26740/jrba
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and biological education (Innovation of Biology Learning, Assessment and Evaluation in Biology, and Media of Biology)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 125 Documents
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Prevalensi Kejadian Aglutinasi Spermatozoa Rattus norvegicus Strain Wistar Albino yang Diberi Paparan Antibiotik Kanamycin Abeiasa, Muhammad Saka
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 1 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n1.p1-5

Abstract

Kelompok antibiotik dari golongan aminoglikosida seperti kanamycin dan gentamycin menyebabkan kesalahan penerjemahan dan efek inhibisi pada proses translokasi t-RNA dan m-RNA bakteri. Dilaporkan bahwa toksisitas kanamycin dapat meningkat seiring dengan peningkatan dosis yang diterima. Hal ini dapat berakibat penurunan jumlah dan kecacatan spermatozoa. Penelitian ini bertujuan untuk menguji pengaruh antibiotik kanamycin terhadap prevalensi kejadian aglutinasi spermatozoa. Jumlah aglutinasi spermatozoa dihitung menggunakan agglutination grade WHO. Penelitian ini merupakan penelitian eksperimental dengan model post-test-only control group design dengan variabel independen yaitu kanamycin dosis bertingkat dan variabel dependen yaitu jumlah aglutinasi spermatozoa. Data dianalisis menggunakan ANAVA dan  dilanjutkan dengan  uji lanjut Bonferroni. Hasil penelitian menunjukkan bahwa pemberian kanamycin dosis bertingkat berpengaruh terhadap prevalensi kejadian aglutinasi spermatozoa. Peningkatan jumlah aglutinasi seiring dengan peningkatan dosis yang diterima.
Diversity and Community Structure of Dragonfly and Damselfly (Odonata) at the Selorejo Waterfall Area, Ponorogo Regency, East Java Indonesia Susanto, Muhamad Azmi Dwi; Zulaikha, Siti
Jurnal Riset Biologi dan Aplikasinya Vol 3, No 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p30-37

Abstract

Selorejo Waterfall is a natural tourism area that is quite far from downtown Ponorogo and directly adjacent to the Gunung Sigogor Nature Reserve. Hence, this area has the potential as a natural habitat for dragonfly and damselfly. The presence of dragonfly and damselfly species is determined by the type of habitat, canopy conditions, vegetation diversity, and the microclimate that exists in a location. This study aimed to determine the diversity of dragonfly and damselfly and to determine the community structure of dragonflies in Selorejo Waterfall. The sampling method was Visual Day Flying. The results of research conducted in two streams showed that there were 12 species from 6 families with a total of 230 individuals. The value of species diversity at this location is H '= 2.05. In the community structure at the Selorejo Waterfall dragonfly, there are differences in the number of species and individuals in the two streams in Selorejo Waterfall Area. The large stream, eight species from four families, 151 individuals in total. Meanwhile, in small stream, there were nine species from six families, 79 individuals in total. The differences in the number of species and individual dragonflies in the two streams at Selorejo Waterfall can be used to describe the diversity and structure of the Odonata community in the area.
Keanekaragaman Burung di Kawasan Hutan Mangrove Banyuurip Kecamatan Ujungpangkah Kabupaten Gresik Mubarrok, Muhammad Musthofa; Ambarwati, Reni
Jurnal Riset Biologi dan Aplikasinya Vol 1, No 2 (2019)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v1n2.p54-63

Abstract

Kawasan hutan Mangrove Banyuurip Ujungpangkah Gresik memiliki potensi dalam mendukung keanekaragaman burung, namun terjadi penurunan dan alih fungsi lahan. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis burung, menganalisis keanekaragaman dan kemelimpahan burung serta mendeskripsikan daya dukung lingkungan terhadap keanekaragaman burung di kawasan hutan mangrove Banyuurip. Sampling menggunakan pendekatan birdwatching dengan metode jelajah dengan berjalan sesuai jalur transek yang telah ditentukan sepanjang ± 150 meter yang terbagi menjadi tiga titik pengamatan. Keanekaragaman dianalisis menggunakan indeks keanekaragaman Shannon-Wiener, kemelimpahan dianalisis berdasarkan kemelimpahan relatif, dan daya dukung lingkungan dianalisis berdasarkan vegetasi dan faktor fisik lingkungan yang meliputi suhu dan kelembapan. Hasil penelitian menunjukkan bahwa di kawasan Hutan Mangrove Banyuurip terdapat 35 jenis burung yang termasuk 20 famili dan delapan ordo, yaitu Anseriformes, Apodiformes, Charadriiformes, Ciconiiformes, Columbiformes, Coraciiformes, Passeriformes, dan Piciformes dengan nilai indeks keanekaragaman sebesar 2,3 yang termasuk dalam kategori sedang. Jenis burung yang paling melimpah adalah kuntul kecil (Egretta garzetta) sebesar 39,25%, blekok sawah (Ardeola speciosa) sebesar 14%, dan walet linci (Collocalia linchi) sebesar 7,8%. Selain itu, diketahui tujuh jenis vegetasi yang dominan dimanfaatkan dan mendukung keberadaan burung, yaitu Avicennia marina, Azadirachata indica, Rhizophora apiculata, R. mucronata, Calotropis gigantea, Morinda citrifolia, dan Pluchea indica. Kawasan hutan mangrove Banyuurip dapat mendukung kehidupan burung, bahkan di kawasan ini ditemukan enam jenis burung dalam status dilindungi.
Analysis Condition of Coral Reef Covering in Pramuka Island Waters, Seribu Islands using Line Intercept Transect (LIT) Method Permana, Rega; Akbarsyah, Nora; Putra, Pringgo KDNY; Andhikawati, Aulia
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 2 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n2.p77-81

Abstract

The coral reef ecosystem is one of the typical tropical ecosystems with high biodiversity which has an important role both biologically, ecologically, physically as well as socially and economically. Several coral reef areas in Indonesia were reported to have suffered damage, not only due to climate change which has an impact on rising sea surface temperatures and ocean acidification but also due to anthropogenic factors and irresponsible management of marine tourism. This study aimed to analyze the condition of coral reefs based on covering in Pramuka Island, which is one of the famous tourist destinations in the Seribu Islands. The study was conducted using Line Intercept Transect (LIT) method at predetermined coordinate points. The results showed that the dominant coral reef life form was Acropora Submassive (ACS), namely 18.9%, and Acropora Branching (ACB) as much as 12.48%. Besides, the types of life forms found were coral foliase (9.42%), Miliepora Coral (9.2%), Coral Massive (4.8%), Acropora Encrustring (4.24%), and so on. Based on the results of this study, it can be concluded that the condition of coral reefs in Pramuka Island is still relatively good with a total coral cover percentage of 72.38%. Efforts to protect the coral reef ecosystem in this area need to be considered so that its condition can be maintained. 
Keanekaragaman dan Kelimpahan Jenis Burung di Kawasan Cagar Alam Besowo Gadungan dan sekitarnya Kabupaten Kediri Jawa Timur Wulandari, Eka Yosida; Kuntjoro, Sunu
Jurnal Riset Biologi dan Aplikasinya Vol 1, No 1 (2019)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v1n1.p18-25

Abstract

Cagar Alam Besowo Gadungan yang terletak di Kabupaten Kediri merupakan salah satu dari delapan belas wilayah cagar alam yang ada di provinsi Jawa Timur. Cagar alam ini termasuk dalam kawasan konservasi dengan tipe ekosistem hutan hujan tropis daratan rendah. Tujuan penelitian ini adalah mengevaluasi tingkat keanekaragaman, menganalisis kelimpahan jenis burung dan mengevaluasi daya dukung lingkungan di kawasan Cagar Alam Besowo Gadungan dan sekitarnya. Metode yang digunakan adalah jalur transek dengan menentukan lima stasiun pengamatan. Pengambilan data dilakukan enam kali pada bulan Maret 2018 serta dua kali pengamatan yaitu pagi dan sore pukul 07.00-10.00 WIB dan 14.00-17.00 WIB. Burung yang dijumpai diidenitifikasi berdasarkan ciri morfologi dan jumlah. Berdasarkan ciri dan jumlah akan dihitung menggunakan indeks keanekragaman Shannon-Wienner dan kelimpahan relatif. Hasil penelitian menunjukkan kawasan Cagar Alam Besowo Gadungan terdapat 38 jenis burung dengan indeks keanekeragaman 2,64 kategori sedang. Jenis yang paling melimpah adalah Collocalia linchi (38,20%), Pycnonotus aurigaster (10,11%) dan Pericrocotus cinnamomeus (5,62%). Vegetasi yang mendukung keberadaan burung pada kawasan ini adalah Albizia chinensis, Aleurites moluccanus, Alstonia scholaris, Altingia excelsa, Coffea arabica, Hopea odorata, Ficus annulata, Ficus benjamina dan Pinus merkusii. Faktor fisik yang mendukung kehadiran burung pada pagi hari hingga sore hari suhu rata-rata 25,9-28,9ºC; kecerahan sebesar 1833,77-2279,68 Lux dan kelembaban udara 56,23-60,97%.
Addition of Carbon Sources to Pineapple Waste Media in the Production of Single Cell Protein Biomass Saccharomyces cerevisiae Nurmalasari, Anggi; Maharani, Shinta
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 2 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n2.p70-76

Abstract

Single-cell protein (SCP) is the term used for crude or pure protein derived from simple single or multi-celled microorganisms. Pineapple peel contains monosaccharides as much as 10.8% so that it can be used as a fermentation medium in single-cell protein production. The purpose of this study was to determine the effect of adding carbon sources of fructose and sucrose on pH, cell dry weight, and protein content in the manufacture of single-cell proteins. This study used a completely randomized design (CRD) with two factors, namely the addition of carbon (fructose, sucrose, and control) and fermentation time (24.48, and 72 hours). The data analysis used the variance test and the Duncan Multiple Range Test (DMRT) continued to test with a confidence level of 95%. The results showed that the addition of carbon to the media had a very significant effect on media pH, cell dry weight, and protein content. In the medium with the addition of fructose it has a pH of 3.81; dry weight 0.4203 grams; and protein content 69.08/L. Whereas in the media with the addition of sucrose, the pH was 4.33, the dry weight of the cells was 0.3385 grams, and the cells had a protein content of 85.55 mg/L. The addition of a fructose carbon source gave the cell dry weight more than the addition of carbon sucrose
Optimasi Waktu Induksi dalam Mengekspresikan Gen Proinsulin secara Intraseluler Menggunakan Inang Pichia pastoris Martius, Efrida; Laoditta, Febraska; Sofia, Dewi Yustika; Mahsunah, Anis Herliyanti
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 1 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n1.p26-35

Abstract

Gen proinsulin telah berhasil diinsersi pada galur Pichia pastoris X33, GS115 dan KM71H pada penelitian sebelumnya, namun masih memerlukan optimasi. Penelitian ini dilakukan untuk mengetahui ekspresi galur P. pastoris dan waktu induksi yang optimal dalam mengekspresikan proinsulin. Ekspresi proinsulin dilakukan pada suhu 20 °C selama 120 jam untuk pemilihan galur terbaik. Variasi lama induksi yang digunakan adalah 0, 24, 48, 72, 96, 120 dan 144 jam untuk mengetahui waktu induksi optimal. Kultur kemudian dilisis, dielektroforesis menggunakan Tricine SDS PAGE dan divisualisasikan dengan pewarnaan perak. Berdasarkan ketebalan pita yang terbentuk pada gel elektroforesis, galur X33-X2 menghasilkan proinsulin terbanyak dibandingkan GS115-G11 dan KM71H-K4.. Ekspresi proinsulin optimal pada 120 jam dan mengalami penurunan proinsulin pada waktu induksi 144 jam akibat akumulasi metanol. Galur X33-X2 merupakan inang terbaik dan waktu induksi 120 jam merupakan waktu induksi optimal dalam mengekspresikan proinsulin secara intraseluler. Hasil ini diharapkan dapat menjadi referensi dalam mengembangkan produksi insulin di Indonesia.
The Comparison of SARS-CoV-2, SARS-CoV, and MERS-CoV Genome and Spike Protein Variations Fikriani, Choirun Nita; Ardana, I Kade Karisma Gita; Listyorini, Dwi
Jurnal Riset Biologi dan Aplikasinya Vol 3, No 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p38-44

Abstract

SARS-CoV-2 is a virus that has caused COVID-19 pandemic. This virus is a new variant of the SARS-CoV virus and also closely related to MERS-CoV, which caused similar acute respiratory infections. All these viruses recognize target cells by binding to the Receptor Binding Domain (RBD) on Spike protein with receptors. This study aimed to determine the SARS-CoV-2, MERS-CoV, and SARS-CoV genome structure, Spike protein sequence differences, and variations of RBD’s Receptor Binding Motif (RBM). This research was using data mining approach. Genome sequences were downloaded from NCBI, except for Indonesian samples were downloaded from GISAID. Genomic structures, Spike sequence, and RBD structure were analyzed using Bioedit, followed by protein modelling using SwissModel and PyMol applications. The result showed that the SARS-CoV-2, MERS-CoV, and SARS-CoV genome shared the same genes yet in different numbers and length. SARS-CoV-2 Spike protein sequence was quite similar to SARS-CoV Spike protein, but very different to the Spike protein of MERS-CoV. There were variations of RBD’s RBM structure due to the mutations occurred among these viruses. It is suggested that these differences may increase the affinity between SARS-CoV-2 Spike protein to its hACE2 receptor which caused SARS-CoV-2 becomes more infective and spread wider than SARS-CoV and MERS-CoV, in turn. This result expected to be basic information for the development of SARS-CoV-2 introduction inhibition agent and spreading prevention.
Training Students’ Attitudes in Environmental Science Course Through Lesson Study Rahmadiarti, Fida; Kuntjoro, Sunu; Budijastuti, Widowati
Jurnal Riset Biologi dan Aplikasinya Vol 1, No 1 (2019)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v1n1.p40-46

Abstract

The core competences of Environmental Sciences are to communicate and to understand the concepts of natural resources and environment, to solve related problems, and to have an environmental awareness. An effective effort is needed to train the environmental awareness. The purposes of this research were to evaluate how to train the attitudes of biology education study program students in the second semester who were taking the environment knowledge lecture. This research was quantitative and qualitative interpretation research by observing the learning process of Biology education students through lesson study. The research stages consisted of plan, do, and see in two cycles. The collected data included the results of observation on the learning process by the observer, the lecturers’ assessment related to the skills and attitudes during the learning process, and the students’ self-assessment on their attitudes. The data were analyzed descriptively. The research revealed that the students learned actively, they cooperated within groups, the class cleanliness was an indicator of attitude to environmental awareness because the lecturers always reminded the students related to the indicators of keeping the environment clean and treating garbage, the learning resources used challenged the students to work and discuss. The students’ attitudes towards environmental awareness are categorized as good-very good, the results of the lecturers’ assessment of the attitudes are categorized as good, and results of the students’ presentation skills are categorized as good-very good.
Defining the Rearing Cage for Agriocnemis femina Damselfly (Odonata, Zygoptera, Coenagrionidae) Janra, Muhammad Nazri; Herwina, Henny; Rahmayani, Hafizhah; Rahmawati, Lily; Sehati, Dika Putri; Fandesti, Shania Refka
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 2 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n2.p42-48

Abstract

Rearing insects such as dragonflies and damselflies aim to gain uniform progeny that used for scientific purposes. In Indonesia, unfortunately, this rearing type is not yet common which suggests the time for its initiation. This study has objective to define the type of rearing cage for Agriocnemis femina damselfly (Odonata, Zygoptera, Coenagrionidae). It was conducted descriptively by using two smalls (9 x 13 x 23 cm), four medium (14 x 15 x 22 cm) and two larges (20 x 23 x 33 cm) size boxes as cage setups, with or without ornamental plants in it. The feeding was with limited (10-15 Drosophila flies provided per day) and unlimited provision. Data was analyzed descriptively. The results showed that A. femina lived normally, including eating and mating, within the large cage setup equipped with ornamental plants and unlimited feeding.

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