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Dwi Anggorowati Rahayu
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Jurnal Riset Biologi dan Aplikasinya
Published by Universitas Negeri Surabaya
ISSN : -     EISSN : 26559927     DOI : 10.26740/jrba
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and biological education (Innovation of Biology Learning, Assessment and Evaluation in Biology, and Media of Biology)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 125 Documents
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Pemberian EM4 (Effective Microorganisme 4) Hasil Kultur dalam Media yang Berbeda pada Pakan untuk Budi daya Lele (Clarias sp.) Anis, Mohhamad Yunus; Hariani, Dyah
Jurnal Riset Biologi dan Aplikasinya Vol 1, No 1 (2019)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v1n1.p1-8

Abstract

Ikan lele (Clarias sp.) merupakan salah satu komoditas perikanan air tawar yang banyak diminati di Indonesia dan terjadi peningkatan produksinya setiap tahun. Untuk itu, terus dipacu peningkatan produksi ikan lele. Upaya yang dilakukan untuk meningkatkan produksi ikan lele yaitu melalui pemberian EM4 (Effective microorganism 4) pada pakan. EM4 yang digunakan berisi Lactobacillus casei dan Saccharomyces cerevisiae. EM4 dikultur dalam media yang dibuat dari molase, bekatul, susu sapi, buah nanas, kunyit putih, temulawak, jahe merah dan air kelapa. EM4 hasil kultur dalam media disebut sebagai fermentor. Penelitian ini bertujuan untuk menguji pengaruh pemberian EM4 hasil kultur dalam media yang berbeda pada pakan terhadap laju pertumbuhan spesifik/spesific growth rate (SGR), rasio konversi pakan/feed conversion ratio (FCR) dan tingkat kelangsungan hidup/survival rate (SR) benih ikan lele. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakuan terdiri atas A (pakan komersial), B (pakan+10% EM4), C (pakan+10% fermentor 1), D (pakan+10% fermentor 2) dan E (pakan+10% fermentor 3), setiap perlakuan diulang sebanyak empat kali. Pakan difermentasi selama 1-3 hari sebelum pakan diberikan kepada benih ikan lele ukuran panjang 7-9 cm. Data dianalisis menggunakan ANAVA. Hasil penelitian menunjukkan bahwa pemberian EM4 hasil kultur dalam media yang berbeda pada pakan berpengaruh secara signifikan terhadap SGR, FCR dan SR benih ikan lele (P<0,05). Perlakuan terbaik diperoleh pada perlakuan E (pakan+10% fermentor 3) dengan nilai SGR sebesar 5,91±0,04%, FCR sebesar 0,88±0,045 dan SR sebesar 73,50±1,91%.
Difference of Red Blood Cell Count (RBC) Levels in Diabetes Mellitus Type II with Ulcers and without Ulcers Mushlih, Miftahul
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 1 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n1.p6-10

Abstract

Type II diabetes mellitus (T2DM) is a metabolic disorder that has potential causing ulcer complications. Ulcers occur due to peripheral vascular abnormalities and trauma. The occurrence of ulcers allows differences in the hematology characteristics in sufferers. This study aimed to determine the comparison of the blood picture between T2DM with ulcers and T2DM without ulcers. This was analytical descriptive research using 29 samples (10 T2DM samples with ulcers and 19 T2DM samples without ulcers). A complete blood test was performed using Sysmex Kx-21N hematology analyzer. Data were analyzed using Independent T-test and Mann-Whitney U depends on the normality. Normality was done using Shapiro Wilk (Confident Level: 95%). The results of this study indicated that Red blood cell count (RBC) and Hemoglobin Count (HGB) differ significantly between T2DM with ulcers and T2DM without ulcers (p: 0.012 and 0.006). The identification of HGB levels was highly recommended to get proper treatment in T2DM.
Aklimatisasi Tanaman Hasil Eksplorasi Tahura R. Soerjo dan Pulau Yamdena di Kebun Raya Purwodadi Rahadiantoro, Apriyono; Indahsari, Nita Dwi
Jurnal Riset Biologi dan Aplikasinya Vol 1, No 2 (2019)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v1n2.p87-91

Abstract

Kebun Raya Purwodadi sebagai lembaga konservasi exsitu yang mengoleksi berbagai jenis tumbuhan terutama dataran rendah kering kembali mengirimkan tim eksplorasi di beberapa kawasan hutan di Indonesia. Tanaman hasil eksplorasi tersebut sebelum menjadi tanaman koleksi membutuhkan proses adaptasi terhadap kondisi lingkungan baru yang dikenal dengan istilah aklimatisasi tanaman. Proses tersebut dapat berlangsung lama dan menjadi salah satu tahapan yang kritis dalam konservasi tumbuhan dikarenakan adanya tingkat kelangsungan hidup tanaman. Tujuan penelitian ini adalah menentukan tingkat ketinggian hidup tanaman dari Tahura R. Soerjo dan Pulau Yamdena serta mempelajari faktor-faktor yang mempengaruhinya. Berdasarkan hasil penelitian diketahui bahwa tingkat kelangsungan hidup tanaman di kedua lokasi tersebut berbeda. Tanaman Tahura R. Soerjo memiliki tingkat kelangsungan hidup yang lebih tinggi dibandingkan Pulau Yamdena. Berdasarkan analisis, faktor-faktor yang mempengaruhi tingkat kelangsungan hidup tanaman pada kedua lokasi tersebut lebih dikarenakan oleh kondisi iklim mikro lokasi asal dibandingkan zona ketinggiannya. Sedangkan untuk jenis material tanaman masih dibutuhkan penelitian lebih lanjut.
Screening of Extracellular Enzymes on Serratia marcescens strain MBC1 Putri, Meishy Handerlin; Handayani, Kusuma; Setiawan, Wawan A; Damayanti, Berliana; Ratih, Cindy Lukyta; Arifiyanto, Achmad
Jurnal Riset Biologi dan Aplikasinya Vol 3, No 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p23-29

Abstract

Hydrolase enzymes are a class of enzymes used to break down the organic substrates into simpler molecules. Hydrolase enzymes play a role in biocontrol, industry, and public health. One of the hydrolase enzyme producers is bacteria. Serratia marcescens strain MBC1 is a collection in the Microbiology Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung which enzymatic activities haven’t been known yet. This research aimed to determine the enzymatic activity of S. marcesescens strain MBC1 that can be used as a candidate for biocontrol agents, biosurfactant producers, industry, or in the health sector. The screening was carried out using a qualitative method plate assay using selective solid media for each test, with three replications for each test. The data obtained were analyzed statistically using analysis non-parametric kruskall-wallis test. The lipolytic activity of the S. marcescens strain MBC1 showed high efficacy with an enzymatic index of 5.52 and amylase, cellulases, protease, mannanase, and chitinase with enzymatic indexes, respectively, 4.17, 1.50, 0.69, 0.35, and 0.27. This enzyme activity of this isolate can be used as a candidate for biological control agents,biosurfactant producers, industry, or the health sector.
Jenis-jenis Gulma Pada Kebun Tebu di Kecamatan Asembagus, Situbondo, Jawa Timur: Kelompok Eudikotiledon Hariri, Muhammad Rifqi; Irsyam, Arifin Surya Dwipa
Jurnal Riset Biologi dan Aplikasinya Vol 1, No 2 (2019)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v1n2.p47-53

Abstract

Agroekosistem perkebunan tebu tidak akan pernah lepas dari kehadiran gulma sebagaimana telah dipaparkan oleh Backer dalam Atlas of 220 weeds of sugarcane fields in Java pada tahun 1973. Hingga saat ini, informasi mengenai gulma-gulma di perkebunan tebu belum terhimpun secara utuh sedangkan kehadiran gulma-gulma terkini semakin banyak dilaporkan. Penelitian ini bertujuan untuk inventarisasi jenis-jenis gulma perkebunan tebu di lima desa yang terletak di Kecamatan Asembagus dilakukan menggunakan metode jelajah. Sebanyak 97 jenis gulma kebun tebu yang termasuk ke dalam 27 suku ditemukan di Kecamatan Asembagus. Sepuluh jenis di antaranya belum dicatat oleh Backer sebagai gulma pada kebun tebu.
The Phenetic Relationship of Ferns (Polypodiaceae) at the Ascent of Cemoro Kandang, Mount Lawu Sianturi, Advend Sri Rizky
Jurnal Riset Biologi dan Aplikasinya Vol 2, No 2 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v2n2.p64-69

Abstract

The fern is one of the highly diverse vascular plant species, which has a unique characteristic. The closely related at the family level because the genera within each family have some uniformity so that the relationship and groupings cannot be distinguished. One example of a diverse fern family is Polypodiaceae. Consequently, it is necessary to simplify the classification; one of them is through phenetic relationship analysis. This study aimed to analyze the phenetic relationship of the Polypodiaceae ferns in Climbing Cemoro, Mouth Lawu enclosure, Karangayar Regency, based on the similarity index on the dendogram. The data were collected using the exploration method and the sporophyte morphological characters were observed. The observations obtained 13 variations of characters. The relationship analysis was carried out with the hierarchical cluster program using SPSS 23. There were six species found from the Polypodiaceae family, there were including Belvisia mucronata, Goniophlebium sp, Lepisorus sp, Pyrrosia piloselloides, Crypsinus taeniatus and Drynaria sparsisora. The finding suggests that six species are divided into two clusters, the Group I consisting of Goniophlebium sp, Lepisorus sp, Lepisorus sp, and Pyrrosia pilloselloides with a similarity coefficient index of 53% and Group II consisting of Drynaria sparsisora, Goniophlebium sp, and Pyrrosia piloselloides with a similarity index of 46%.
Perbandingan Morfologi Adipose-Derived Stem Cells asal Donor Diabetes Melitus Tipe 2 dalam Medium Mengandung Platelet-Rich Plasma dan Fetal Bovine Serum Karina, Karina; Rosadi, Imam; Sobariah, Siti; Rosliana, Iis; Wahyuningsih, Komang A; Widyastuti, Tias; Afini, Irsyah
Jurnal Riset Biologi dan Aplikasinya Vol 1, No 2 (2019)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v1n2.p64-70

Abstract

Salah satu terapi luka pada diabetes melitus tipe 2 adalah terapi sel punca. Lingkungan mikro bagi sel termasuk sel punca, dapat rusak akibat komplikasi dari diabetes. Lingkungan mikro yang rusak tersebut dapat menyebabkan penuaan (senescent) dini pada sel punca. Studi ini bertujuan untuk menganalisis pengaruh morfologi adipose-derived stem cells (ADSCs) dari donor diabetes mellitus tipe 2 dalam medium yang mengandung platelet-rich plasma (PRP). Tahapan studi yang dilakukan yaitu menghitung variasi densitas awal ADSCs. Densitas awal untuk kultur ADSCs adalah 5,000; 10,000; dan 20,000 sel pada setiap kelompok. Hasilnya menunjukkan bahwa morfologi ADSCs dalam medium PRP umumnya lebih kecil dibandingkan morfologi ADSCs dalam medium FBS pada berbagai jumlah densitas awal ADSCs yang dikultur. Morfologi ADSCs kelompok PRP didapatkan semakin kecil luas morfologinya pada densitas kultur awal sel yang besar (20,000 = 0,014 mm2; 10,000 = 0,016 mm2; 5,000 = 0,018 mm2) begitu juga kelompok FBS (20,000 = 0,032 mm2; 10,000 = 0,032 mm2; 5,000 = 0,036 mm2). Luas ukuran ADSCs yang dikultur menggunakan FBS jauh lebih besar dibandingkan PRP dan berbeda bermakna pada densitas jumlah sel awal yang dikultur sebanyak 20,000 sel (p <0,05). Berdasarkan hasil tersebut menunjukkan bahwa morfologi ADSCs yang dikultur dengan kerapatan densitas 20,000 sel dalam medium PRP memiliki ukuran sel yang lebih kecil secara signifikan dibandingkan medium FBS.
Distribution Patterns of Exotic Plant Chromolaena odorata, in Rehabilitation Zone at Donglo Block, Resort of Wonoasri, Meru Betiri National Park Rizkiah, Dwi Wardatul; Sulistiyowati, Hari; Siddiq, Arif Mohammad
Jurnal Riset Biologi dan Aplikasinya Vol 3, No 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p1-6

Abstract

Exotic plants are plants that are introduced intentionally or unintentionally from their original habitat to a new habitat. One of the exotic plants that is potentially invasive is Chromolaena odorata. These plants are found a lot especially in the rehabilitation zone at Donglo Block Resort of Wonoasri, Meru Betiri National Park (MBNP). An initial effort to determine whether this exotic plant was potentially invasive was to use the distribution pattern of a plant population. This study aimed to determine the distribution pattern and area of cover of exotic plants C. odorata in rehabilitation zone at Donglo Block, Resort of Wonoasri MBNP. The sampling of C. odorata used the transect plot method systematically, which carried out in Donglo Block Resort of Wonoasri MBNP. Data analyzed using Morisita index. The map of distribution pattern of C. odorata was created using the Geographic Information System (GIS). Based on the results of the Morisita index, the value (Iδ) = 12.39, which means that C. odorata has a clumped distribution pattern. The distribution pattern of C. odorata can also be seen from the visualization of the spatial distribution map, which shows that the growth of C. odorata in plants or overlaps with each other, hence that it looks clustered. The coverage area of C. odorata in the study location was 596,5 m2 or 29.24% of the total area of the study (20,400 m2). Based on the results of the coverage area of C. odorata, it was indicated that this exotic species is not yet classified as an invasive species in the area.
Diversity of Lepidoptera: Rhopalocera at Selorejo Waterfall Area, Ponorogo District, East Java, Indonesia Zulaikha, Siti; Susanto, Muhamad Azmi Dwi
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p68-72

Abstract

Selorejo Waterfall is a natural tourist area that is directly adjacent to the Sigogor Mountain Nature Reserve and has a beautiful and natural environment. This makes the Selorejo Waterfall area has a good environment for the sustainability of various species of butterflies. This research aimed to find out the diversity and abundance of butterflies in the Selorejo Waterfall area. This research was conducted using the Visual Day Flaying method by following the trail in the Selorejo Waterfall area. This study found 26 species belongs 4 families with 109 individuals in total. The value of the species diversity index at the location of H'= 2.81 moderate category. The diversity of butterflies in the Selorejo Waterfall area was influenced by the availability of host plants and feed as well as abiotic factors.
S-D614G Mutation Reveals the Euro-America and East-Asia Origin SARS-CoV-2 Virus Spread in Indonesia Anggraini, Nina Bunga; Listyorini, Dwi
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p45-53

Abstract

COVID-19 is a pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. The first case was found in the city of Wuhan, Hubei province, China. The first case in Indonesia was reported in March 2020 and currently there are 0.5 million cases with a death rate of 3.1%. This rapid increase in cases is thought to due to presence of the mutant strain S-D614G, which causes a faster rate of infection and spread. The purpose of this study was to determine the presence of S-D614G mutations in Indonesian samples in order to find the origin of COVID-19 which was spread in Indonesia based on the Spike gene sequences and the RdRp genes from 25 countries, and one control sequence China/Wuhan-Hu-1 obtained from the NCBI and GISAID databases. Mutation analysis was carried out through multiple alignments using BioEdit software. Phylogenetic tree reconstruction using MEGA6 software with the Neighbor Joining method. This study found mutation of S-D614G in one Indonesian sample, namely the Indonesian/SBY9 sample along with 23 samples from Europe, America, and Africa. The phylogenetic tree reconstruction confirmed these findings; the mutated samples were closely related to samples from these continents, while the non-mutated Indonesian samples were closely related to sample from East Asia. These findings indicate that the origin of the SARS-CoV-2 virus in Indonesia possibly came from the East Asia cluster and the European-American cluster.

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