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Arif Rahman Hikam
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Fakultas Biologi Universitas Jenderal Soedirman Jl. dr. Soeparno No. 63 Purwokerto, Kabupaten Banyumas Kode Pos 53122
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 401 Documents
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POTENSI EKSTRAK ETIL ASETAT Coprinus comatus TERHADAP KADAR SGOT DAN SGPT PADA TIKUS PUTIH MODEL DIABETES Feryawan Feryawan; Nuniek Ina Ratnaningtyas; Nuraeni Ekowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4238

Abstract

Coprinus comatus or shaggy in cap mushroom contain bioactive compounds including flavonoids, alkaloids, terpenoids, tocopherols, comatin, and ergotionin which function as antioxidants and antidiabetic. Diabetes mellitus is a chronic disease associated with impaired insulin secretion or decreased insulin sensitivity which causes blood glucose levels to rise above normal (hyperglycemia). Hyperglycemia conditions can causes oxidative stress and increase Reactive Oxygen Species (ROS), to trigger lipid peroxidation which damages the pancreas, kidneys and liver. One way to determine liver damage is by measuring the increase in liver enzyme levels of Serum Glutamate Oxaloacetate Transaminase (SGOT) and Serum Glutamate Pyruvate Transaminase (SGPT) in the bloods. The aims in this study is determine the effect of administration and determine the effective doses of ethyl acetate C. comatus extract with different doses on the levels of SGOT and SGPT in the bloods of diabetic rats. The results showed that C. comatus ethyl acetate extract could reduce SGOT and SGPT levels as well as streptozotocin-induced rat bloods glucose levels. Ethyl acetate C. comatus extract with doses 500 mg / kg BW is the most effective doses in reducing SGOT and SGPT levels in the bloods of streptozotocin-induced rats with a mean SGOT level of 57,96 ± 1,58 U/L and SGPT levels of 29,67 ± 3,56 U/L, and the percentage reduction in SGOT and SGPT levels compared to negative controls respectively was 43,15% and 68,70%, and blood glucose was 19,62%.
Prevalensi Infestasi Cacing Gastrointestinal Pada Kambing (Capra sp.) Yang Dipelihara Secara Intensif Dan Semi Intensif Aulia Khirqah; Rokhmani Rokhmani; Edy Riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4224

Abstract

The main purpose of this study are to compare gastrointestinal worm species founds in goats, to compare prevalence and intensity in goats under intensive and semi intensive rearing system and to compare sex-wise and age related prevalence and intensity of gastrointestinal worms in goat. This research was conducted in intensive and semi intensive goat farms in Desa Klareyan, Kecamatan Petarukan, Pemalang using survey method with purposive random sampling technique in November 2020. Gastrointestinal worms species found in goat feces under intensive rearing system are Strongyloides sp., Haemonchus sp., Oesophagostomum sp., Trichostrongylus sp., and Ascaris sp. while species founded in goats under semi intensive rearing system are Strongyloides sp., Haemonchus sp., Oesophagostomum sp., Trichostrongylus sp., Ascaris sp., Moniezia expansa, Moniezia benedeni, Fasciola sp., And Schistosoma sp. The intensity of gastrointestinal worm eggs in goat under intensive rearing system was 1184.44±1743,28, its higher than the intensity in goats under semi intensive rearing system which has 1109.50±1573.73 (P> 0.05). The prevalence of gastrointestinal worm infestations in goats under intensive rearing system is 90%, lower than the goats under semi intensive rearing system which has 100% prevalence (Sign. 0.147). Gastrointestinal worms prevalence and intensity in male goats is lower than female goats (Sign. 0,189; P>0,05), while Gastrointestinal worms prevalence and intensity in lamb goat is higher than young goat and adult goat (Sign. 0,241; F<0,05).
KAJIAN ETNOBOTANI TANAMAN Curcuma longa L. DAN Curcuma zanthorrhiza ROXB. OLEH MASYARAKAT BOGOR JAWA Malia Ulfah; Wardah Wardah; Rusmadi Rusmadi; Muhammad A'tourrohman
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4287

Abstract

The community of Bogor, West Java are a group of people who still apply the traditional pattern of life. The use of turmeric plants (Curcuma longa L.) and temu lawak (Curcuma zanthorrhiza Roxb.) by local people is still ongoing today. This research was conducted to determine the economic value of turmeric and temulawak for the people of Bogor, the use and use of turmeric and temu lawak plants, and the role of ethnobotany for the development of science. The method used in this study is data collection techniques in the form of literature studies, observations, interviews, and documentation. The results show that turmeric and temu lawak have economic potential for the people of Bogor, West Java. Its uses include medicinal plants, food additives and as vegetables. How to use it is to be mashed, cut and consumed directly. Revitalizing community knowledge about the use of plants around is very important for the development of science.
Keanekaragaman KEANEKARAGAMAN SPESIES BURUNG DIURNAL DI CAGAR ALAM NUSAKAMBANGAN TIMUR Iftah Sadjad Ahmadi; Suhestri Suryaningsih; Erie Kolya Nasution
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4233

Abstract

Birds are members of a group of vertebrate animals belonging to the aves class. Birds play an important role in ecosystem components to support an organism's life cycle. The high diversity of bird species is supported by the high diversity of habitats that function as places for finding food, drinking, resting and breeding. Nusakambangan is an island with an area of ​​240 km2 with lowland natural forest, coastal forest and mangrove forest. Research in 2003 and 2004 western part of Nusakambangan has 93 bird species and in 2006, bird species in the type of habitat for sandy coastal forest, pamah forest, limestone forest, grasslands, young shrubs, and old shrubs contained 121 species of birds. This study aims to determine the diversity of bird species in the Nusakambangan Timur Nature Reserve. This research was conducted using a survey method with a point count technique. The data were analyzed by simple descriptive and then displayed in tabular form. There are 46 bird species from 25 families with a diversity index of 2.5456 and a dominance index of 0.1027.
Prevalensi dan Intensitas Tungau Parasit pada Kucing Peliharaan yang Diperiksakan di beberapa Klinik Hewan Purwokerto Amelia Nurma Hidayah; Bambang Heru Budianto; Hery Pratiknyo
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4564

Abstract

This study aimed to determine the prevalence and intensity of parasitic mites in domestic cats at several veterinary clinics in Purwokerto. This research was conducted by cross sectional method using random sampling technique at three veterinary clinics in Purwokerto City. The identification of parasitic mites was analyzed descriptively, the data on the mites found were then analyzed using the prevalence and intensity formulas. The prevalence and intensity figures obtained were then compared with the prevalence and intensity category tables. The results of the study reported that the total prevalence of parasitic mites was 85%, which was included in the category of prevalence usually with moderate infestation, with a total intensity of parasitic mites of 196.7 which included in the category of very severe intensity. Species of parasitic mites found include Otodectes cynotis, Notoedres cati and Lynxacarus radovskyi.
Keragaman Tumbuhan yang Dimanfaatkan Sebagai Pewarna Alami Batik Di Kabupaten Banyumas Makdalena Viviliani Sir; Wiwik Herawati; Sukarsa Sukarsa
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4592

Abstract

Natural dyes are compounds that can be produced from various types of natural sources. Plants that produce natural dyes can be obtained from their parts such as leaves, bark, fruit skin, seeds, roots and flowers, which have gone through several processes, namely boiling, burning, crushing, pounding and immediately used. This study aims to determine the types of plants used as natural dyes for batik, and to determine which parts of plant organs are used as natural dyes for batik. The method used in this research is a survey method. Sampling was done by purposive sampling. Data were collected by observing and interviewing the batik home industry in Banyumas Regency. The parameters used are the types of plants and plant organs that are used as natural dyes for batik. Data were tabulated and identified for analysis. Analysis of the research data was carried out descriptively. Plant used as natural batik dyes in several Batik Home Industries in Banyumas Regency consists of 12 plant species the parts of plants were roots, stems, leaves, tubers, wood, bark, fruit skin, and coir. Natural dyes of batik are obtained by boiling and pounding. Then Used by Soaking, Dipping, and to draw motifs.
The Effect Of Date Palm Extract (Phoenix dactylifera L.) On Orchid Plantlets (Vanda tricolor Lind) Growth using In Vitro Culture Resita Nursechan; kamsinah kamsinah; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4227

Abstract

Vanda tricolor Lindl. is widespread in Java, Bali, and Sulawesi, V. tricolor on natural habitat became scarce due to forest destruction and natural disasters and human activities. To prevent the extinction it can be done by the propagation of orchids using in vitro culture. The addition of dates extract (Phoenix dactylifera L.) containing carbohydrates to increase growth and differentiation of cells in orchids. This study aimed to determine the effect of date palm extract on the growth of V. tricolor L. plants and determine the best dose of date palm extract on the growth of V. tricolor plantlets. This research was conducted experimentally with a Completely Randomized Design (CRD). The addition of dates on Vaccint & Went / VW media consisting of 6 treatments, that are : 0 g/L, 50 g/L, 100 g/L, 150 g/L, 200 g/L, 250 g/L, each treatment repeated 3 times so there are 18 trial units. The source of the explants used was orchid plantlet (V. tricolor L.). The parameters include the number of roots, root length, number of leaves, plantlet height, and leaf length. The research data has been analyzed using Analysis of variance (ANOVA) with a confidence level of 95% and 99%. The result of this research showed that the addition of date palm extract give a result not significant for the number of leaves, the number of roots, the longest leaf length, the longest root length, the plantlet height of the vanda tricolor orchid in vitro.
Karakterisasi dan Optimasi Aktivitas Bakteriosin Isolat Bakteri Asam Laktat LG-90 Asal Sedimen Mangrove Pantai Logending Fiqita Mayliani; Dyah Fitri Kusharyati; Dini Ryandini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4575

Abstract

Lactic acid bacteria (LAB) are a group of Gram-positive bacteria that produce various active metabolites, including lactic acid, ethanol, hydroperoxides, and bacteriocins. Bacteriocin is a peptide that has a broad spectrum to inhibit the growth of pathogenic microbes. The ability of bacteriocins to inhibit microbial growth is influenced by various factors, including the concentration of antimicrobial substances, temperature, storage time, pH, and microbial properties. LG-90 isolated from mangrove sediments at Logending Beach located in Ayah Village, Ayah District, Kebumen Regency, is known to be capable of producing bacteriocins. This research aimed to determine the characteristics and identity of LG-90, the optimum time of bacteriocin production, and the optimum pH and temperature for bacteriocin activity of LG-90 as antimicrobial agents. This research used a survey method. The independent variable in this research was LAB LG-90 isolates and the dependent variable were the ability to produce bacteriocins and their antibacterial power. The main parameter observed was diameter of the inhibition zone and the supporting parameters were morphological, physiological, and biochemical properties of bacteria. Descriptive data analysis and characterization of bacterial isolates refers to Bergey’s Manual of Systematic Bacteriology. The results showed that LG-90 from the mangrove sediments of Logending Beach had the similar phenetic characters as the genus Lactobacillus. Optimum bacteriocin production of LG-90 at an incubation time of 16 hours. Optimum antimicrobial activity of LG-90 bacteriocin at pH 6 and heating temperature of 40oC.
Aktivitas Amilolitik Bakteri Sedimen Mangrove Pantai Logending, Ayah, Kebumen pada Suhu dan pH Berbeda Alfiani Rahmawati; Oedjijono .; Dini Ryandini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4574

Abstract

The main purpose of this study wereto determine the ability of mangrove sediment bacterial isolates to produce amylase, to determine the optimum pH and temperature on the amylase activity, and to identify theamylase-producing bacteria. The research was conducted by survey method. The research stages included, screening of amylolytic bacteria, growth curvespreparation, amylase production, optimizing amylolytic activity at varies oftemperature (35ºC; 36ºC; 37ºC; 38ºC) and pH (4.5; 5; 5.5; 6) and characterization of amylolytic isolates. Parameters measured were amylolytic index, amylase enzyme activity unit, total bacterial unit, and identity of amylolytic bacteria from mangrove sediments. Data analysis was done descriptively. The results showed that the highest amylolytic index were shown by isolate LG113 with an amylolytic index value of 9,86 mm from 10 amylolytic isolates from mangrove sediments. The optimum temperature of amylase activity was 37ºC(2,13 U/mL) and the optimum pH was 6(2,14 U/mL). The total number of amylolytic bacterial cells at the end of production time was 1,94x1013 CFU/mL. The identity of isolates of amylolytic bacteria from mangrove sediments at the Logending beachwas belong toBacillus genus.
Identifikasi dan UJi Potensi Amilolitik Isolat Jamur Pendegradasi Sampah Organik Insaaniy Mahdiyatul Haqq; Ratna Stia Dewi; Aris Mumpuni; Arif Rahman Hikam; Dwiana Muflihah Yulianti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4748

Abstract

Sampah organik merupakan sampah yang tersusun dari senyawa organik. Penumpukan sampah organik cukup menjadi masalah serius. Jamur memiliki peran penting untuk mendegradasi sampah organik dalam proses pengomposan. Biodegradasi sampah organik berkaitan erat dengan kemampuan jamur dalam menghidrolisis senyawa amilum. Tujuan dari penelitian ini yaitu mengetahui isolat jamur pendegradasi amilum yang diperoleh dari sampah organik dan mengetahui potensi amilolitik dari isolat jamur tersebut. Penelitian ini dilakukan dengan metode survei dan eksperimental . Sampel sampah dapur yang terdiri atas sisa makanan dan bahan organik lainnya diambil dari rumah penduduk yang terletak di Kelurahan Bancarkembar, Bobosan, Grendeng, Karangwangkal, Pabuaran, Purwanegara, dan Sumampir. Tes screening menggunakan medium Starch Agar untuk mengetahui potensi amilolitik dari isolat jamur. Hasil menunjukkan terdapat delapan isolat jamur yang berpotensi dalam mendegradasi amilum. Sebanyak enam isolat jamur yang memiliki indeks amilolitik terbaik dengan nilai IE ≥ 1 teridentifikasi sebagai isolat Fusarium sp., Aspergilus sp., dan Penicillium sp. Selanjutnya untuk mengetahui aktivitas amilolitik secara kuantitatif dilakukan dengan metode DNS melalui pengukuran kadar glukosa. Isolat jamur Fusarium sp. memiliki aktivitas degradasi amilum yang paling tinggi dengan rata-rata kadar glukosa dari medium Fusarium sp. sebanyak 3.568,63 ppm

Page 13 of 41 | Total Record : 401

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