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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 401 Documents
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FITOREMEDIASI MENGGUNAKAN KAYU APU (Pistia stratiotes) UNTUK MENURUNKAN KADAR COD LIMBAH CAIR TEKSTIL Utami Sari Dewi; Slamet Santoso; Elly Proklamasiningsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4375

Abstract

Waste water treatment is needed to avoid the impact of pollution to the aquatic environment. Techniques for treating waste water, among others, are through phytoremediation using the help of water lettuce (Pistia stratiotes).This research aims to determine the effect of the percent covering area of water lettuce, the length of exposure time, and interaction of both in reducing levels COD, and to determine the percent of covering area, the length of exposure time, and interaction of both that is the most efficient in reducing The result of this research showed that the percent covering area, and the length of exposure time had a very significant effect of decreasing levels of COD. The most efficient percent covering area in reducing COD was in treatment of 75%. The most efficient length of exposure time in reducing COD was in treatment 9 days. The most efficient interaction of both in reducing COD levels was at treatment of 100% coverage area with exposure time of 9 days.
PENAMBAHAN IAA DAN BAP TERHADAP PERTUMBUHAN KALUS EKSPLAN DAUN ANGGREK Coelogyne pandurata Lindl. Johanes De Britto Bintan Cahyo Adi; Kamsinah Kamsinah; Lucky Prayoga
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4255

Abstract

Anggrek Coelogyne pandurata Lindl atau anggrek hitam merupakan anggrek endemik dari Kalimantan. Anggrek ini mempunyai keunikan yakni mempunyai sepal berwarna hijau serta labelum yang berwarna hitam. Perbanyakan secara alami tingkat keberhasilannya sangat kecil maka perlu di lakukan kultur in vitro. Salah satu cara kultur in vitro adalah kultur menggunakan eksplan daun untuk memacu pertumbuhan kalus. Pertumbuhan kalus dapat dipacu dengan auksin (IAA) dan sitokinin (BAP). Perpaduan antara konsentrasi auksin dan sitokinin yang seimbang diharapkan bisa memacu pertumbuhan kalus. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan IAA dan BAP terhadap pertumbuhan kalus anggrek C. pandurata Lindl dan mengetahui interaksi IAA dan BAP yang terbaik terhadap pertumbuhan kalus anggrek C. pandurata Lindl. Metode penelitian dilakukan secara eksperimental dengan rancangan acak lengkap menggunakan pola faktorial , faktor 1 kosentrasi IAA dengan 4 taraf : 0, 1, 2, 3 mg/L. Dan faktor ke 2 konsentrasi BAP dengan 4 taraf perlakuan : 0, 1 , 2, 3 mg/L, sehingga ada 16 perlakuan , dan masing-masing perlakuan diulang 3 kali sehingga ada 48 unit percobaan. Parameter yang diamati adalah : waktu munculnya kalus, tebal kalus, jenis kalus, berat kalus dan presentasi terbentuknya kalus. Data yang diperoleh dianalisis menggunakan ANOVA dan dilakukan uji lanjut DMRT dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan pemberian IAA dan BAP dapat mempengaruhi pertumbuhan kalus eksplan daun anggrek Coelogyne pandurata Lindl. Pengaruh secara interaksi terdapat pada wantu munculnya kalus, tebal kalus dan Prosentase hidup kalus. Serta mampu memacu perkembangan kalus ke arah proliferatif. Sedangkan pemberian IAA secara mandiri dapat memacu penebalan kalus. Interaksi yang terbaik dalam pertumbuhan kalus eksplan daun anggrek Coelogyne pandurata Lindl. adalah A2B2 (IAA 2 mg/L dan BAP 2 mg/L) dan A2B3 (IAA 2 mg/L dan BAP 3 mg/L).
BIOMASS AND CHLOROPHYLL ANALYSIS OF Ceriops tagal IN DIFFERENT NURSERY MEDIA Herna Maghfira Raudhatul Jannah; Erwin Riyanto Ardli; Elly Proklamasiningsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4545

Abstract

Ceriops tagal is a major mangrove species that plays an important role in the mangrove ecosystem. Ceriops tagal is one type of mangrove that has benefits for various marine organisms such as fish, shrimp, nematodes, etc also can maintain the stability of the mangrove ecosystem. In rehabilitation, it is necessary to have a nursery. The addition of husk and husk charcoal to the nursery media can increase the absorption space for water, air, and plant nutrients. The research aims to (1) determine the effect of differences in nursery media with a mixture of husk and husk charcoal on mud media; (2) determine which nursery media has the best effect on biomass and chlorophyll content of C. tagal mangroves. This research started from planting the propagules of C. tagal in different composition growing media with husk and husk charcoal then calculated with ANOVA test 5% and 1% of the biomass in root, stem, leaves, and hypocotyl also chlorophyll content in leaves of C. tagal organ. Then proceed with the honest significant difference test 5% for the wet biomass of root, dry biomass of stem, root, and wet also dry biomass hypocotyl of C. tagal. Research obtained that modification of nursery media with husk and husk charcoal can increase dry biomass of root and stem also wet and dry biomass of hypocotyl in C. tagal organs. The media best media for increase the biomass of the C. tagal in each organ was media 5 (composed of mud + husk charcoal in ratio 2:1) and media 6 (composed of mud + husk + husk charcoal in ratio 1:1:1). The increasing dry biomass of root and stem also wet and dry biomass of hypocotyl are also affected by environmental factors such as pH content in the medium.
Keanekaragaman Serangga pada Tanaman Cabai Merah Di Desa Kubangwungu Kecamatan Ketanggungan Kabupaten Brebes Dwi Yuliani; Devi Ayu Susilowati; Anton Maulana Ibrahim
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4278

Abstract

The increasing number of population and the growing food industry that requires raw material for red chilies have led to an increase in demand for red chilies. The research was conducted using a survey method which was carried out from February 2021 to April 2021. Sampling of insect diversity was carried out on 1,000 m2 of red chili plantations. The land for the red chili plants was determined by 10 points randomly. Each sampling point consisted of 2 pitfall traps, 1 light trap, and 1 insect net with 4 replications. Insect sampling was carried out when the chili plants began to enter the generative period (44-50 days after planting) for 6 consecutive days, in this phase the insect pests began to be seen in many chili plants. The results of the sampling were then identified using the J. Borror (1992) determination key book. Analysis of insect diversity data was carried out by calculating the diversity index (diversity) based on Magurran (1988) using the Shannon-Evenness Index and the Shannon-Evenness Index. The results showed that 28 species consisted of the order hemiptera, coleopteran, prthoptera, diptera, hymenoptera, odonata, mantodea, and Lepidoptera. The results showed that the diversity index of insect pollinators on red chili agricultural land using the pit fall method (H´ = 2.874) was lower than the light (H '= 3.029) and netting methods (H' = 3.0159), while the evenness index on land The pit fall method of red chili farming (E = 0.8627) was lower than the light method (E = 0.909) and netting method (E = 0.9481). Keywords: diversity, insects, red chilies
Kajian Anatomi Daun dan Morfologi Nepenthes spp. Koleksi Kebun Raya Baturaden Kabupaten Banyumas Camelina Simbolon; Siti Samiyarsih; Wiwik Herawati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4560

Abstract

Pitcher plant (Nepenthes spp.) is one of the carnivorous plants which is classified as unique, because of the pitch on the Nepenthes spp. is a modified leaf tip that changes its shape and function to become a trap for insects or other small animals. The Baturraden Botanical Garden is the areas used for the conservation of various plant species both ex-situ and in-situ including Nepenthes spp. This aim of this study is to 1) determine the leaf anatomy and morphology characters of Nepenthes spp. which grows in the Baturraden Botanical Garden area, 2) determine the different characters of each species of Nepenthes spp. which grows in the Baturraden Botanical Garden area based on leaf anatomy and morphology. The research was conducted by direct observation, purposive sampling method, taking leaf samples of Nepenthes spp. in the greenhouse of the Baturaden Botanical Garden. Observation of leaf anatomical characters by embedding method with 1% safranin staining. The results of the measurement of anatomical and morphological characters were analyzed using ANOVA at the 95% and 99% confidence level, then continued with tests of Least Significant Difference (LSD) and the result observation from the morphological observations were analyzed descriptively. The results showed that the eight Nepenthes species had the same anatomical structure consisting of cuticle, epidermis, and mesophyll tissue with varying sizes between species. The morphological characters of eight Nepenthes species varied among species with different sizes. The petiole is only owned by N. ampullaria, N. mirabilis, N. rafflesiana, the pitch that has a waist shape is only owned by N. mirabilis, N, gracilis, and N. tobaica, the most striking pitch color is N. rafflesiana. The differences in the leaf anatomy characters of the eight Nepenthes species were found in the stomata width, cuticle thickness, epidermal thickness, mesophyll thickness, leaf length, leaf width, leaf stalk, tendril length, pitch diameter, pitch shape, pitch color
Struktur Komunitas Fitoplanton di Waduk Cacaban Kabupaten Tegal Dian Setyowati; Diana Retna Utarini Suci Rahayu; Agatha Sih Piranti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4247

Abstract

Phytoplankton has an important role in an aquatic ecosystem because it is autotrophic and also a source of natural food for fish. Phytoplankton will interact with other biotic factors to form a community structure that is useful for the sustainability of the ecosystem Therefore, this research is needed to determine the phytoplankton community structure in Cacaban Reservoir because the community structure can shows the water conditions. The purpose of this reserch was to determine the composition, abundance, and diversity of phytoplankton at each station and the most dominant type of phytoplankton in Cacaban Reservoir. The result showed that there are 18 species of phytoplankton in Cacaban Reservoir. The four phytoplankton divisions found were Chlorophyta with 8 species, Bacillariophyta with 5 species, Cyanophyta with 3 species, and Charophyta with 2 species. The abundance is in the medium category, with the abundance of Chlorella sp. the highest at stations I, III and IV; and Microcystis sp. at stations II and V. The diversity index ranges between 1,227-1,766 and was in the low category which indicates low community stability. The dominance index range between 0,648-0,791 and was in the high category. The dominant phytoplankton in the Cacaban Reservoir is from the Chlorophyta division, which is also a type favored by fish.
KUALITAS BIJI DAN KARAKTER AGRONOMI PADI HITAM (Oryza sativa L.) LOKAL PEKALONGAN DENGAN PENAMBAHAN PAKLOBUTRAZOL DAN GIBERELIN Endang Triyani Prihantari; Triani Hardiyati; Siti Samiyarsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4240

Abstract

Black rice (Oryza sativa L.) is a rice variety that has many additional functions and is used as a functional food. However, black rice has a longer harvest period and has a higher apparent stem height than other types of rice so that black rice plants are easy to break. This will reduce the quality of rice plants. One of the methods that can be done to overcome this problem is treating by paclobutrazol and gibberellin. The combination of paclobutrazol and gibberellin is expected to provide better rice growth and improve the quality of rice seeds. The purpose of this study was to determine the effect of the addition of paclobutrazol and gibberellin on seed quality, palea-lemma size and agronomic character of local Pekalongan black rice (Oryza sativa L.). The research was conducted experimentally using a completely randomized design (CRD) of factorial treatment pattern. The first factor was paclobutrazol with 4 levels of concentration (0 ppm, 100 ppm, 200 ppm and 300 ppm) applied once at 4 weeks of plant age and the second factor was gibberellin with 4 levels of concentration (0 ppm, 100 ppm, 200 ppm and 300 ppm ) applied once at the age of 6 weeks of plants, so that are 48 combinations with each treatment was replicated 3 times. The parameters observed included plant height, leaf width, flowering age, harvest age, palea-lemma size, weight of 100 seeds, protein and amylose content. The data obtained were analyzed using the ANOVA test at the 95% confidence level, and continued with the Honestly Significant Difference (HSD) test at an error rate of 5%.The results showed that the interaction between paclobutrazol and gibberellin was not significant, but treatment of 300 ppm paklobutrazol showed the best results in plant height inhibition, while the treatment of paclobutrazol 200 ppm was the best concentration to stimulate flowering and harvesting age. Treatment of gibberellin 100 ppm was found the best concentration to increase leaf width while a concentration of 300 ppm proved to give the best results for palea-lemma size, amylose content, protein content and weight of 100 seeds per plant.
POTENSI EKSTRAK ETIL ASETAT Coprinus comatus TERHADAP KADAR UREUM DAN KREATININ PADA TIKUS PUTIH MODEL DIABETES Anik Laeli Perdanawati; Nuniek Ina Ratnaningtyas; Hernayanti Hernayanti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4239

Abstract

Coprinus comatus or chicken drumstick mushrooms has potential as antioxidant and antidiabetic. Hyperglycemia in people with diabetes mellitus causes an increase in Reactive Oxygen Species (ROS). Pancreatic β cells have less antioxidants than other organs. This causes oxidative stress which triggers a chain reaction of lipid peroxidation which damages the kidneys and disturbs the glomerular filtration rate. Impaired glomerular filtration rate is characterized by increased levels of urea and creatinine. Flavonoids in C. comatus are able to donate H + and stop lipid peroxidation reactions in the kidneys, so that urea and creatinine levels decrease.This study aims to determine the effect of C. comatus ethyl acetate extract on blood urea and creatinine levels in diabetic rats and to determine the effective dose of C. comatus ethyl acetate extract on blood urea and creatinine levels in diabetic rats. This study used an experimental method based on a completely randomized design (CRD). The data obtained from the measurement of urea and creatinine levels were analyzed using the one way Anova statistical test at the 95% confidence level and followed by Duncan's test at an error rate of 5%. The results of this study indicated that the ethyl acetate extract of C. comatus affected the blood urea and creatinine levels of diabetic rats. C. comatus extract at a dose of 500 mg/kgBW is an effective dose that has an effect on reducing levels of blood urea and creatinine in the amount of 16,66 ± 0,00 mg/dL dan 0,40 ± 0,12 mg/dL.
Karakteristik Morfologi Post Larva Udang Penaeus dan Metapenaeus dari Kawasan Timur Segara Anakan Cilacap Harditya Firdhaus; Dian Bhagawati; Kusbiyanto Kusbiyanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4554

Abstract

Information about the presence of post larvae in nature could indicate the specific presence of the sites used for growth and development of larvae and post larvae. This could be an alternative option to determine the diversity of shrimp in an area with the presence of post larvae. Research about species richness of post larvae phase of shrimp based on morphology characters, especially in the eastern region of Segara Anakan Cilacap is still rarely done, so it is necessary to collected basic data on the morphological characters of post larvae phase of shrimp. The purpose of this research is to knew species richness and morphological description post larvae phase of shrimp in the eastern region of Segara Anakan, Cilacap Regency, Central Java. The method used survey with a random sampling technique from the collection of the Laboratory of Animal Taxonomy, Faculty of Biology, Jenderal Soedirman University. The variables in this research were morphological performance, standar morphometric, and meristic of post larvae shrimps. The parameters observed were characters of morphological description on each post larvae phase of shrimp, ratio standard morphometric, and meristic calculation. Species richness, morphological performance, standard morphometric, and meristic data were analyzed descriptively. The results obtained were three species of post larvae of Family Penaeidae namely Penaeus indicus, P. merguiensis, and Metapenaeus ensis.
Detection of Psychotropic Compound in Coprophilous Fungi in District of Baturraden Banyumas Regency Safira Amanda; Aris Mumpuni; Nuraeni Ekowati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.4245

Abstract

Coprophilous fungi or dung loving fungi are a group of fungi adapted to life on dung and fecal pellets of herbivorous animals. Coprophilous fungi contain psychotropic compounds. Psychotropics compounds are substances or drugs, both natural and synthetic, not narcotics, which have psychoactive properties through a selective influence on the central nervous system which causes distinctive changes in mental activity and behavior. Chemical Spot Test still remain an important tool for the preliminary identification of illicit drugs and other psychotropic compound in spite of developments in instrumental technology and the increased portability of this technology which enables its use in the field. Banyumas Regency is a very potential area as a habitat for coprophilous fungi, specifically in Baturraden District because there are many cattle farms where the dung is where the fungi grow, also the climate is suitable for fungal growth. make an inventory and identify the coprophilous fungi found in District of Baturraden Banyumas Regency and to detect the presence of psychotropic compound in the fungi. The research will be conducted using purposive random sampling and Color Test or Chemical Spot Test analysis. The obtained data is analyzed descriptively by comparing with Atlas of The Munsell Color System. This research obtained seven genera of coprophilous fungi i.e: Coprinellus sp., Coprinopsis sp., Entoloma sp., Gymnopus sp., Lepiota sp., Parasola sp. and Stropharia sp. that discovered in two cattle farms in Baturraden District.

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